Microbial Genomics
... How do we measure the levels of all the different mRNA transcripts ? When the genome sequence is known a set of DNA molecules which correspond to each gene can be synthesized. These a spotted on a glass slide and this is called a DNA micro-array because the spots are very small and can only be seen ...
... How do we measure the levels of all the different mRNA transcripts ? When the genome sequence is known a set of DNA molecules which correspond to each gene can be synthesized. These a spotted on a glass slide and this is called a DNA micro-array because the spots are very small and can only be seen ...
20 - Biotechnology
... the biotechnology tools that make cloning possible. The key ideas that make PCR possible. How gel electrophoresis can be used to separate ...
... the biotechnology tools that make cloning possible. The key ideas that make PCR possible. How gel electrophoresis can be used to separate ...
Biotechnology
... the biotechnology tools that make cloning possible. The key ideas that make PCR possible. How gel electrophoresis can be used to separate ...
... the biotechnology tools that make cloning possible. The key ideas that make PCR possible. How gel electrophoresis can be used to separate ...
Reference - Human Microbiome Journal Club
... Adaptors contain amplification- and sequencing primer binding sites; platform- and chemistry-specific Optional: sample-specific barcodes/indexes/MIDs/tags allow ...
... Adaptors contain amplification- and sequencing primer binding sites; platform- and chemistry-specific Optional: sample-specific barcodes/indexes/MIDs/tags allow ...
Genetic Engineering Poster
... The DNA of pigs has been modified using recombinant DNA technology so their cells develop without certain genes which trigger the human immune response. The hope is that these genetically ...
... The DNA of pigs has been modified using recombinant DNA technology so their cells develop without certain genes which trigger the human immune response. The hope is that these genetically ...
Unit 2 Review
... Use the following as a TOOL only. Survey to see what you don’t know, focus on these terms in your notes. This will not be covered in class other than process questions you and several others are concerned about. Questions that are straight from the notes will be re-directed to the notes—find them th ...
... Use the following as a TOOL only. Survey to see what you don’t know, focus on these terms in your notes. This will not be covered in class other than process questions you and several others are concerned about. Questions that are straight from the notes will be re-directed to the notes—find them th ...
Program of Agricultural Microbiology (pdf version)
... and rhizobes), and with animals (rumen, intestinal tract). Criteria and methods in bacterial taxonomy Laboratory course outline Basic aseptic techniques. Streak-plate technique, spread and pour plating, plate counts, isolation to obtain pure cultures. Optical observations of wet mount preparations. ...
... and rhizobes), and with animals (rumen, intestinal tract). Criteria and methods in bacterial taxonomy Laboratory course outline Basic aseptic techniques. Streak-plate technique, spread and pour plating, plate counts, isolation to obtain pure cultures. Optical observations of wet mount preparations. ...
Document
... to explore the roles, relationships, and actions of the various types of molecules that make up the cells of an organism. technologies include: Genomics, “the study of genes and their function” (Human Genome Project (HGP), 2003) Proteomics, the study of proteins. ...
... to explore the roles, relationships, and actions of the various types of molecules that make up the cells of an organism. technologies include: Genomics, “the study of genes and their function” (Human Genome Project (HGP), 2003) Proteomics, the study of proteins. ...
Supplementary Data 1 (doc 909K)
... (CL) were EGFR-F: 5’-AAAAGGCAGTGGCTGAATTG-3’, EGFR-R: 5’AGGCGGTGGTTACGAGTATG-3’, ECOP-F: 5’AAGACAGACATGTAGACCAATGGA-3’, ECOP-R: 5’GCTGAGAGATGGAAGCAACC, CL-F: 5’-TCCATCCTTTATGTTTGGGTTC, CL-R: 5’GGGACACGTGTAACAAAATCAG. The control region was selected by examining the SNP array data for a copy number ...
... (CL) were EGFR-F: 5’-AAAAGGCAGTGGCTGAATTG-3’, EGFR-R: 5’AGGCGGTGGTTACGAGTATG-3’, ECOP-F: 5’AAGACAGACATGTAGACCAATGGA-3’, ECOP-R: 5’GCTGAGAGATGGAAGCAACC, CL-F: 5’-TCCATCCTTTATGTTTGGGTTC, CL-R: 5’GGGACACGTGTAACAAAATCAG. The control region was selected by examining the SNP array data for a copy number ...
summary slides
... Additional tests 1. Differential staining 2. Biochemical tests- determine presence of enzymes - Numerical identification 4. Genetic homology (similarity of DNA) - Base composition - DNA and RNA sequencing (16s rRNA gene) - DNA hybridization 5. Protein and amino acid homology (similarity of proteins) ...
... Additional tests 1. Differential staining 2. Biochemical tests- determine presence of enzymes - Numerical identification 4. Genetic homology (similarity of DNA) - Base composition - DNA and RNA sequencing (16s rRNA gene) - DNA hybridization 5. Protein and amino acid homology (similarity of proteins) ...
(PCR) and Gel Electrophoresis Powerpoint
... • A sample which contains fragments of DNA is forced by an electrical current through a firm gel which is really a sieve with small holes of a fixed size – Phosphate group in DNA is negatively charged so it is moved towards a positive electrode by the current – Longer fragments have more nucleotides ...
... • A sample which contains fragments of DNA is forced by an electrical current through a firm gel which is really a sieve with small holes of a fixed size – Phosphate group in DNA is negatively charged so it is moved towards a positive electrode by the current – Longer fragments have more nucleotides ...
Jeff Newman - Davidson College
... • Connie Wilson – “figure out relationships between different species - two species in same environment both adapted to the conditions but in different ways.” • Jen Leader – “They can be compared to eukaryotes which will aid in structural and functional identification of proteins/genes.” • Justin Ja ...
... • Connie Wilson – “figure out relationships between different species - two species in same environment both adapted to the conditions but in different ways.” • Jen Leader – “They can be compared to eukaryotes which will aid in structural and functional identification of proteins/genes.” • Justin Ja ...
Document
... result in the breakage [hydrolysis] of the sugarphosphate bond between certain specific nucleotide bases [recognition sites]. This causes the double strand of DNA to break along the recognition site and the DNA molecule becomes fractured into two pieces. These molecular scissors or “cutting” enzymes ...
... result in the breakage [hydrolysis] of the sugarphosphate bond between certain specific nucleotide bases [recognition sites]. This causes the double strand of DNA to break along the recognition site and the DNA molecule becomes fractured into two pieces. These molecular scissors or “cutting” enzymes ...
Food Safety and Beyond
... match it with complementary nucleotides very quickly. The result is two new helixes in place of the first, each composed of one of the original strands plus its newly assembled complementary strand. ...
... match it with complementary nucleotides very quickly. The result is two new helixes in place of the first, each composed of one of the original strands plus its newly assembled complementary strand. ...
BioRad #166-0007EDU: Forensic DNA Fingerprinting Checklist PREP
... Technicians working in forensic labs are often asked to do DNA profiling (fingerprinting) to analyze evidence in law enforcement cases and other applications. Restriction Fragment Length Polymorphism (RFLP) has been the workhorse of forensic DNA profiling for many years. First described by English g ...
... Technicians working in forensic labs are often asked to do DNA profiling (fingerprinting) to analyze evidence in law enforcement cases and other applications. Restriction Fragment Length Polymorphism (RFLP) has been the workhorse of forensic DNA profiling for many years. First described by English g ...
Zoo/Bot 3333
... blot analysis. The probe used in this instance hybridizes to a DNA fragment linked to the disease gene, which shows polymorphism for this restriction enzyme. The autoradiogram of this blot is shown above, aligned with the family pedigree. 5. In the above example, which of the following are likely t ...
... blot analysis. The probe used in this instance hybridizes to a DNA fragment linked to the disease gene, which shows polymorphism for this restriction enzyme. The autoradiogram of this blot is shown above, aligned with the family pedigree. 5. In the above example, which of the following are likely t ...
DNA Technology
... restriction enzyme is used to cut the circular DNA molecule (plasmid) found in bacteria (pink). The same enzyme is then used to cut the DNA that is required (blue). The sticky ends of each will then join up (with the help of another enzyme), inserting the required gene into the plasmid. This techniq ...
... restriction enzyme is used to cut the circular DNA molecule (plasmid) found in bacteria (pink). The same enzyme is then used to cut the DNA that is required (blue). The sticky ends of each will then join up (with the help of another enzyme), inserting the required gene into the plasmid. This techniq ...
Biotechnology - Cobb Learning
... Transfer (SCNT) or *An early stage embryo is split into cells before those cells have differentiated, the cells are then grown separately, and develop into identical embryos and can be implanted into surrogate ...
... Transfer (SCNT) or *An early stage embryo is split into cells before those cells have differentiated, the cells are then grown separately, and develop into identical embryos and can be implanted into surrogate ...
Biotechnology
... • To check the recombinant plasmid, researchers might cut the products again using the same restriction enzyme • To separate and visualize the fragments produced, gel electrophoresis would be carried out • This technique uses a gel made of a polymer to separate a mixture of nucleic acids or proteins ...
... • To check the recombinant plasmid, researchers might cut the products again using the same restriction enzyme • To separate and visualize the fragments produced, gel electrophoresis would be carried out • This technique uses a gel made of a polymer to separate a mixture of nucleic acids or proteins ...