Eukaryotic Gene Expression
... • DNA methylation; the addition of methyl groups to DNA • essential for inactivation of the DNA – Inactive genes in a cell are methylated – Epigenetic memory due to methylating enzymes that methylate the new daughter strand the same as the parent strand. • Can be passed on in repro ...
... • DNA methylation; the addition of methyl groups to DNA • essential for inactivation of the DNA – Inactive genes in a cell are methylated – Epigenetic memory due to methylating enzymes that methylate the new daughter strand the same as the parent strand. • Can be passed on in repro ...
DNA Fingerprinting
... in very specific places in a DNA sequence. Therefore if cut with the same restriction enzyme, the DNA of two different individuals will have different DNA fragment lengths ...
... in very specific places in a DNA sequence. Therefore if cut with the same restriction enzyme, the DNA of two different individuals will have different DNA fragment lengths ...
Schematic courtesy of B. Crump Quantitative (Real Time) PCR
... • Sample diversity deeply and quickly • Find “rare” or low abundance organisms • Limited to short reads (<250bp) ...
... • Sample diversity deeply and quickly • Find “rare” or low abundance organisms • Limited to short reads (<250bp) ...
Curriculum Vitae - Francesca Mapelli Photo
... The scientific and research activity of Dr. Francesca Mapelli covers several areas under the field of microbial ecology and biotechnology, with a focus on extreme ecosystems. The research activity is performed in collaboration with national and international partners. Major research lines are: mic ...
... The scientific and research activity of Dr. Francesca Mapelli covers several areas under the field of microbial ecology and biotechnology, with a focus on extreme ecosystems. The research activity is performed in collaboration with national and international partners. Major research lines are: mic ...
Taxonomic probes
... for the genus Nitrobacter (red stain cluster) correlated to oxygen and nitrate gradients measured by microelectrodes. ...
... for the genus Nitrobacter (red stain cluster) correlated to oxygen and nitrate gradients measured by microelectrodes. ...
BiotechnologySimple
... • introduction of foreign DNA into a bacterial cell • plasmid is used as a vector, a vehicle by which DNA can be introduced into host cell ...
... • introduction of foreign DNA into a bacterial cell • plasmid is used as a vector, a vehicle by which DNA can be introduced into host cell ...
AP Biology DNA Technology: The manipulation of organisms or their
... Restriction fragments can be separated through Gel Electrophoresis in which the fragments are run through a gel containing an electrical field. o DNA runs towards the positive pole because it is negatively charged. o Smaller segments migrate further than large ones. o Every individual has a unique s ...
... Restriction fragments can be separated through Gel Electrophoresis in which the fragments are run through a gel containing an electrical field. o DNA runs towards the positive pole because it is negatively charged. o Smaller segments migrate further than large ones. o Every individual has a unique s ...
Appendix F - WordPress.com
... DNA is negatively charged and is repelled by the negative electrode (cathode) and attracted by the positive electrode (anode) when an electric current is applied across the gel. It separates because different lengths of DNA move through the gel matrix at different rates. Longer fragments move more s ...
... DNA is negatively charged and is repelled by the negative electrode (cathode) and attracted by the positive electrode (anode) when an electric current is applied across the gel. It separates because different lengths of DNA move through the gel matrix at different rates. Longer fragments move more s ...
Microbial diversity
... Sequencing of nucleic acid only way to provide direct comparison of genes/genomes Sequence of 16 S rRNA gene often used to compare organisms 16 S rRNA gene amplified by PCR PCR product sequenced and sequence compared with that of known organism New development: comparative genomics ...
... Sequencing of nucleic acid only way to provide direct comparison of genes/genomes Sequence of 16 S rRNA gene often used to compare organisms 16 S rRNA gene amplified by PCR PCR product sequenced and sequence compared with that of known organism New development: comparative genomics ...
D5 Phylogeny and systematics – summary of mark
... phylogeny / evolutionary history of a species / relationship between species can be determined; proteins analysed for amino acid sequences; more similar the amino acid sequence, the more closely related the species are; eg cytochrome C / hemoglobin / chlorophyll / other valid example; DNA / mitochon ...
... phylogeny / evolutionary history of a species / relationship between species can be determined; proteins analysed for amino acid sequences; more similar the amino acid sequence, the more closely related the species are; eg cytochrome C / hemoglobin / chlorophyll / other valid example; DNA / mitochon ...
Genetic Engineering
... Electrophoresis 1. “Cut” DNA sample with restriction enzymes. 2. Run the DNA fragments through a gel. ...
... Electrophoresis 1. “Cut” DNA sample with restriction enzymes. 2. Run the DNA fragments through a gel. ...
Document
... Chain-termination strand synthesis by a DNA polymerase is illustrated for the G reaction in the figure at left below. To prevent all chains from terminating at the first G position, ddGTP is added at ~1/100th the amount of dGTP. To achieve termination at each type of base, four separate reactions ar ...
... Chain-termination strand synthesis by a DNA polymerase is illustrated for the G reaction in the figure at left below. To prevent all chains from terminating at the first G position, ddGTP is added at ~1/100th the amount of dGTP. To achieve termination at each type of base, four separate reactions ar ...
Southern Blotting
... • Such variations include: ABO blood type, Rhesus factor, and major histocompatibility complex (MHC). • RFLP are differences in homologous DNA sequences that can be detected by the presence of fragments of different lengths after digestion of the DNA. • Moreover, RFLP is a sequence of DNA that has a ...
... • Such variations include: ABO blood type, Rhesus factor, and major histocompatibility complex (MHC). • RFLP are differences in homologous DNA sequences that can be detected by the presence of fragments of different lengths after digestion of the DNA. • Moreover, RFLP is a sequence of DNA that has a ...
The Nature of Things: The Immortal Thread
... Sulston chose this species because : 1. It has specialized cells and organs similar to more complex organisms (such as ourselves). 2. The adult form consists of approximately 1,000 cells. This makes the study of individual cells and the changes they go through, from embryo to adult possible. What ha ...
... Sulston chose this species because : 1. It has specialized cells and organs similar to more complex organisms (such as ourselves). 2. The adult form consists of approximately 1,000 cells. This makes the study of individual cells and the changes they go through, from embryo to adult possible. What ha ...
doc - Genome: The Secret of How Life Works
... 1. Introduce the lesson with a discussion about the unique identity contained in an individual’s genes. 2. Discuss the fact that DNA is like a fingerprint, in that it is unique to each individual and can be used to identify them, even though it is not visible to the naked eye. 3. Have students make ...
... 1. Introduce the lesson with a discussion about the unique identity contained in an individual’s genes. 2. Discuss the fact that DNA is like a fingerprint, in that it is unique to each individual and can be used to identify them, even though it is not visible to the naked eye. 3. Have students make ...
Gene Cloning - Fort Bend ISD
... RFLP Markers: Adjacent to genes are satellite sequences that do not code for anything but vary between individuals. • Because the restriction sites are in different places within the RFLP sequences each person has a DNA “fingerprint”. ...
... RFLP Markers: Adjacent to genes are satellite sequences that do not code for anything but vary between individuals. • Because the restriction sites are in different places within the RFLP sequences each person has a DNA “fingerprint”. ...
explaining the forensic use of dna to the average american
... What parts of the DNA are unique for individuals and easy to measure? It is impractical to every gene in our DNA to the genes of others. Instead what is measured are the “non-sense” genes (codes) that are between each gene. These are called restriction fragment length polymorphism or RFLP ...
... What parts of the DNA are unique for individuals and easy to measure? It is impractical to every gene in our DNA to the genes of others. Instead what is measured are the “non-sense” genes (codes) that are between each gene. These are called restriction fragment length polymorphism or RFLP ...
Bioinformatics
... External, context for your molecule • In other species (homologs, phylog trees) • In which cell • In which cellular location (GO) • Molecular complex (dimers) • Which pathway (KEGG) • Where in genome (neighbors, synteny) ...
... External, context for your molecule • In other species (homologs, phylog trees) • In which cell • In which cellular location (GO) • Molecular complex (dimers) • Which pathway (KEGG) • Where in genome (neighbors, synteny) ...