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Molecular Technologies and Diagnostics
Molecular Technologies and Diagnostics

... •DNA is passed from parent to child, so you can inherit SNPs •Number of matching SNPs can determine family relations ...
Sequence 1 - Human DNA
Sequence 1 - Human DNA

... Write the RNA directly below the DNA strand (remember to substitute U's for T's in RNA) 2. Use the Genetic Code circle OR Genetic Code Table to determine what amino acids are assembled to make the insulin protein in both the cow and the human. Write your amino acid chain directly below the RNA seque ...
2_16S_TREE_RECONSTRUCTION
2_16S_TREE_RECONSTRUCTION

... There are many alignment programs, all look to common features that may indicate homologous sites:  Clustal X  MAFFT  PileUp ...
Evolution & Phylogeny ppt
Evolution & Phylogeny ppt

... – subjective element added. Result: many disputes over proposed phylogenies – not easily resolved (e.g. Brusca’s discussion) ...
Arrays
Arrays

... Many microarray experiments have been performed with Yeast. • Genome is completely sequenced and well annotated. • Select a PCR primer pair that amplifies each ORF. • My arrays- all yeast genes represented (70mers). ...
Characterization and Molecular Identification of Unknown Bacteria
Characterization and Molecular Identification of Unknown Bacteria

... 3 Results and Discussion The traditional identification of bacteria on the basis of phenotypic characteristics is generally not as accurate as identification based on genotypic methods. Comparison of the bacterial 16S rRNA gene sequence has emerged as a preferred genetic technique. The sequence of t ...
DNA Technology Worksheet
DNA Technology Worksheet

... 1. Name two situations when DNA fingerprints are useful. _________________________________ and _______________________________ 2. How does the DNA migrate from one end of the gel to the other? 3. What cuts up the DNA into tiny fragments? _____________________________________ Use the gels below to an ...
DNA Technology Worksheet
DNA Technology Worksheet

... 1. Name two situations when DNA fingerprints are useful. _________________________________ and _______________________________ 2. How does the DNA migrate from one end of the gel to the other? 3. What cuts up the DNA into tiny fragments? _____________________________________ Use the gels below to an ...
042310_recombinant_DNA2
042310_recombinant_DNA2

... copies could be generated) • A recognition sequence for a restriction enzyme (so that we can introduce our DNA of interest) • Reporter genes (to confirm we have successfully introduced the vector into the host cell) • Small size in comparison with host’s chromosomes (for easy manipulation) ...
PLTW Biomedical Competency Profile
PLTW Biomedical Competency Profile

... Isolates DNA from cells Separates DNA fragments by gel electrophoresis Analyzes gel electrophoresis results Uses aseptic technique to sample and transfer bacterial cells Uses proper Gram staining techniques to stain and observe bacteria Performs and analyzes antibiotic efficiency testing Analyzes bo ...
Taxonomic distribution of Large DNA viruses in the sea
Taxonomic distribution of Large DNA viruses in the sea

...  The GOS data comprise a large environmental shotgun sequence collection, with 7.7 million sequencing reads assembled into 4.9 billion bp contigs  At least 3% of the predicted proteins contained within the GOS data are of viral origin  Most DNA samples were extracted from the 0.1-0.8 μsized fract ...
Answers to Biotech Jeopardy
Answers to Biotech Jeopardy

...  Small, circular piece of bacterial DNA is called a ____.  Give two examples of vectors:  The entire collection of genes within human cells is called the _______________.  Difference between technology and biotechnology?  Function of restriction enzymes?  HGP stands for? How many base pairs in ...
Using a Single Nucleotide Polymorphism (SNP)
Using a Single Nucleotide Polymorphism (SNP)

... SNPs • Single Nucleotide Polymorphisms • http://www.youtube.com/watch?v=tJjXpiWKMyA • For a variation to be considered a SNP, it must occur in at least 1% of the population. • SNPs, which make up about 90% of all human genetic variation, occur every 100 to 300 bases along the 3-billion-base human g ...
Om evolution og sekvenser
Om evolution og sekvenser

... Natural Selection: Darwin’s four postulates ...
protocol: restriction endonuclease digestion/analysis of
protocol: restriction endonuclease digestion/analysis of

Ch 10
Ch 10

... • Now that the complete sequence of the human genome is available we know what makes up most of the 98.5% that does not code for proteins, rRNAs, or tRNAs Exons (regions of genes coding for protein, rRNA, tRNA) (1.5%) ...
Promoter Analysis
Promoter Analysis

... occupancy and sets of genes – Estimate TF activity in each condition by ...
Intro + Evolution
Intro + Evolution

... Natural Selection: Darwin’s four postulates ...
Supplementary Information (doc 56K)
Supplementary Information (doc 56K)

... depth significantly explained the variation of pigments, nutrients, extra-cellular enzymes as well as cell abundances (93%, 74%, 80% and 79% of the variation explained, respectively, using multiple regression analyses, own data not shown), which overall confirms previous observations (5). Thus, in t ...
Genetically Modified Organisms and Food All modern agricultural
Genetically Modified Organisms and Food All modern agricultural

... modification, and introduction of DNA into a target organism; when the target organism is a crop plant  or domesticated animal used for food, the purpose is usually to impart to the target organism a desired  trait that is unknown or very difficult to obtain by traditional methods (those in use befo ...
Lecture 16-LC710 Posted
Lecture 16-LC710 Posted

... eGFP (eucaryotic vs for bacterial expression) ...
Name
Name

... eGFP (eucaryotic vs for bacterial expression) ...
Ch 8 Genetic Technology and Diagnostics
Ch 8 Genetic Technology and Diagnostics

... •Hybridization - used to identify bacterial species by analyzing the sequences of nitrogenous bases in DNA ...
ppt slides
ppt slides

... perfect fidelity from separated strands • Strands can be separated (denatured) by heating ...
Project Information
Project Information

...  Sustainable management of biodiversity in forested ecosystems with a focus on management for ecosystem function, resilience and conversation. Projects specialising in invertebrate diversity or plants are particularly welcomed.  Managing saproxylic insects in commercial forest plantations. Prof Pa ...
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