A short ClC-2 mRNA transcript is produced by exon
... Adult rat lung has a decreased long/short form ratio of ClC-2 transcripts Initial agarose gel electrophoresis of the PCR products using HRC5′ and HRC3′ suggested that the brain had the highest ratio of long/short form transcript compared with other tissues, especially the lung and the L2 cells. To q ...
... Adult rat lung has a decreased long/short form ratio of ClC-2 transcripts Initial agarose gel electrophoresis of the PCR products using HRC5′ and HRC3′ suggested that the brain had the highest ratio of long/short form transcript compared with other tissues, especially the lung and the L2 cells. To q ...
マニュアル Megaprime DNA Labelling System
... subsequently removed as monophosphates. Very small amount of input DNA can be labelled, enabling very high specific activity DNA probes to be produced with relatively small quantities of added nucleotides. These radioactive labelled fragments can then be used as sensitive hybridization probes for a ...
... subsequently removed as monophosphates. Very small amount of input DNA can be labelled, enabling very high specific activity DNA probes to be produced with relatively small quantities of added nucleotides. These radioactive labelled fragments can then be used as sensitive hybridization probes for a ...
Molecular Systematics of the Genus Zoogloea and Emendation of
... Proteobucteria, closely related to Agrobacterium tumefmiens. Genomic DNA hybridization studies also showed that there is genetic diversity among the strains currently designated 2. rarnigera, but typical Zoogloeu strains, characterized by their production of rhodoquinones, are highly related to each ...
... Proteobucteria, closely related to Agrobacterium tumefmiens. Genomic DNA hybridization studies also showed that there is genetic diversity among the strains currently designated 2. rarnigera, but typical Zoogloeu strains, characterized by their production of rhodoquinones, are highly related to each ...
Extreme Radiation Resistance by Interdependent DNA
... least two genome copies) due to a DNA repair process accomplishing an efficient and precise fragment assembly2,3,8. This molecular transaction is formally akin to the computer-assisted contig assembly of shotgun sequenced random genomic fragments2. Because D. radiodurans recA and polA are its most r ...
... least two genome copies) due to a DNA repair process accomplishing an efficient and precise fragment assembly2,3,8. This molecular transaction is formally akin to the computer-assisted contig assembly of shotgun sequenced random genomic fragments2. Because D. radiodurans recA and polA are its most r ...
Quantitative real-time PCR assay for rapid detection of
... amplified with RAPD and URP primers failed to produce distinct banding pattern (data not shown). The fingerprinting pattern by URP-9F produced bands 200 bp to 1.5 kb (FIG. 1). One monomorphic band of , 1 kb was observed from all the isolates of M. phaseolina (FIG. 1). This unique and prominent , 1 k ...
... amplified with RAPD and URP primers failed to produce distinct banding pattern (data not shown). The fingerprinting pattern by URP-9F produced bands 200 bp to 1.5 kb (FIG. 1). One monomorphic band of , 1 kb was observed from all the isolates of M. phaseolina (FIG. 1). This unique and prominent , 1 k ...
The effect of human serum DNAases on the ability to detect
... phosphate-buffered saline (PBS). DAPI (10 ìl of a 3 mM suspension in water) was added to 1-ml samples of the bacterial suspension to yield a ®nal DAPI concentration of 30 ìM. The resulting suspension was mixed and incubated at room temperature for 60 min in the dark. Bacteria were centrifuged eight ...
... phosphate-buffered saline (PBS). DAPI (10 ìl of a 3 mM suspension in water) was added to 1-ml samples of the bacterial suspension to yield a ®nal DAPI concentration of 30 ìM. The resulting suspension was mixed and incubated at room temperature for 60 min in the dark. Bacteria were centrifuged eight ...
DpnII - Inv. PCR of miniMos for distribution
... Protocol 2 - Inverse PCR individual inserts There is a very nice and comprehensive protocol that covers how to map Mos1 insertions by Boulin & Bessereau (2007) in Nature Protocols. This protocol is meant as a complement to their protocol because we changed and optimized several parameters which in o ...
... Protocol 2 - Inverse PCR individual inserts There is a very nice and comprehensive protocol that covers how to map Mos1 insertions by Boulin & Bessereau (2007) in Nature Protocols. This protocol is meant as a complement to their protocol because we changed and optimized several parameters which in o ...
Assessing the Homogeneity of Plasmid DNA: An Important
... used to assess the homogeneity of plasmid DNA, but this approach has some major disadvantages. The AGE method is manual, only semi-quantitative, and the assignment of bands to plasmid structures is difficult since the electrophoretic mobility of plasmids of different shapes changes with the electrop ...
... used to assess the homogeneity of plasmid DNA, but this approach has some major disadvantages. The AGE method is manual, only semi-quantitative, and the assignment of bands to plasmid structures is difficult since the electrophoretic mobility of plasmids of different shapes changes with the electrop ...
An improved technique for isolating codominant compound
... genomic sequence has already been registered in the database (Hayden et al. 2004), however. No technique has yet ...
... genomic sequence has already been registered in the database (Hayden et al. 2004), however. No technique has yet ...
video slide - Biology at Mott
... did not result in a uniform width Instead, pairing a purine with a pyrimidine resulted in a uniform width consistent with the X-ray ...
... did not result in a uniform width Instead, pairing a purine with a pyrimidine resulted in a uniform width consistent with the X-ray ...
ISOLATE II PCR and Gel Kit
... PCR primers from reactions are eliminated while small DNA fragments are still bound and purified with high recovery. The cut-off for small DNA fragments can be shifted from <50bp to several hundred base pairs by diluting Binding Buffer CB to remove primer-dimers from target PCR products. A yellow pH ...
... PCR primers from reactions are eliminated while small DNA fragments are still bound and purified with high recovery. The cut-off for small DNA fragments can be shifted from <50bp to several hundred base pairs by diluting Binding Buffer CB to remove primer-dimers from target PCR products. A yellow pH ...
DNA Hybridization: A Decade of Molecular Discourse in Hominoid
... However, they only used one comparison between the tracer DNAs and the driver DNAs, and as a result, they had to conclude that their data could not indicate with any certainty whether the chimpanzee and gorilla are closer to each other than to humans, or if either of them might be closer to humans t ...
... However, they only used one comparison between the tracer DNAs and the driver DNAs, and as a result, they had to conclude that their data could not indicate with any certainty whether the chimpanzee and gorilla are closer to each other than to humans, or if either of them might be closer to humans t ...
Physical and Topological Properties of Circular DNA
... Duplex rings appear to exist in a twisted configuration in neutral salt solutions at room temperature. Examples of such molecules are the DNA's from the papova group of tumor viruses and certain intracellular forms of OX and X-DNA. These DNA's have several common properties which derive from the top ...
... Duplex rings appear to exist in a twisted configuration in neutral salt solutions at room temperature. Examples of such molecules are the DNA's from the papova group of tumor viruses and certain intracellular forms of OX and X-DNA. These DNA's have several common properties which derive from the top ...
A Complete Mitochondrial Genome Sequence
... Summary of Illumina Genome Analyzer Sequencing and mtDNA Sequence Assembly A total of 49,125,583 Illumina Genome Analyzer (GA) single sequence reads, each 36 nucleotides in length, were produced from three independently prepared CPC98 Illumina GA libraries and sequenced across 14 lanes of three flow ...
... Summary of Illumina Genome Analyzer Sequencing and mtDNA Sequence Assembly A total of 49,125,583 Illumina Genome Analyzer (GA) single sequence reads, each 36 nucleotides in length, were produced from three independently prepared CPC98 Illumina GA libraries and sequenced across 14 lanes of three flow ...
The polymerase chain reaction
... temperatures; a degenerate primer is used to amplify in the other direction from the unknown sequence. • Touchdown PCR (Step-down PCR): a variant of PCR that aims to reduce nonspecific background by gradually lowering the annealing temperature as PCR cycling progresses. The annealing temperature at ...
... temperatures; a degenerate primer is used to amplify in the other direction from the unknown sequence. • Touchdown PCR (Step-down PCR): a variant of PCR that aims to reduce nonspecific background by gradually lowering the annealing temperature as PCR cycling progresses. The annealing temperature at ...
Compiling DNA strand displacement reactions using a functional
... for diseases at the cellular level [4]. As the scale and complexity of DNA-based computing devices continues to grow, tool support will become ever more important. A key goal is to formalize the structures and interactions of DNA molecules, so that their behaviour may be analyzed [5]. To this end we ...
... for diseases at the cellular level [4]. As the scale and complexity of DNA-based computing devices continues to grow, tool support will become ever more important. A key goal is to formalize the structures and interactions of DNA molecules, so that their behaviour may be analyzed [5]. To this end we ...
Catabolic Alanine Racemase from Salmonella typhimurium: DNA Sequence, Enzyme Purification, and Characterization.
... DNA sequencing of the dadB gene in plasmid pSWl2 (Wasserman et al., 1983), this plasmid was further subcloned by using DNA manipulations as described by Davis et al. (1980) and by Maniatis et al. (1982), as illustrated in Figure 2 (top). The first step was to delete the 2.7-kb NdeI-NdeI fragment cov ...
... DNA sequencing of the dadB gene in plasmid pSWl2 (Wasserman et al., 1983), this plasmid was further subcloned by using DNA manipulations as described by Davis et al. (1980) and by Maniatis et al. (1982), as illustrated in Figure 2 (top). The first step was to delete the 2.7-kb NdeI-NdeI fragment cov ...
Molecular evidence for the existence of additional members of the
... diagnostic procedures a PCR assay able to detect all known Chlamydiaceae sequences in one reaction was developed. For this purpose, primers were selected to amplify a fragment of the 165 rRNA gene. Characterization of the amplified fragments was done by hybridization with specific probes and by sequ ...
... diagnostic procedures a PCR assay able to detect all known Chlamydiaceae sequences in one reaction was developed. For this purpose, primers were selected to amplify a fragment of the 165 rRNA gene. Characterization of the amplified fragments was done by hybridization with specific probes and by sequ ...
Automated Constraint-Based Nucleotide Sequence Selection for
... To simplify the design of our computational systems, we have developed a tool for constraint-based selection of nucleotide sequences. This tool incorporates domain knowledge that has proven to be important in our experimental process. However, we have also formulated a framework for systematically s ...
... To simplify the design of our computational systems, we have developed a tool for constraint-based selection of nucleotide sequences. This tool incorporates domain knowledge that has proven to be important in our experimental process. However, we have also formulated a framework for systematically s ...
DNA Testing - Who Murdered Robert Wone
... included here primarily for historic reasons (more current formats see below). RFLP DNA testing has four basic steps: 1. The DNA from crime-scene evidence or from a reference sample is cut with something called a restriction enzyme. The restriction enzyme recognizes a particular short sequence such ...
... included here primarily for historic reasons (more current formats see below). RFLP DNA testing has four basic steps: 1. The DNA from crime-scene evidence or from a reference sample is cut with something called a restriction enzyme. The restriction enzyme recognizes a particular short sequence such ...
Replication and Recombinantion
... template for two new strands which then join together, giving two old strands together and two new Dispersive - In strands together which sections of the old strands are dispersed in the new strands ...
... template for two new strands which then join together, giving two old strands together and two new Dispersive - In strands together which sections of the old strands are dispersed in the new strands ...
calibration of tethered particle motion experiments
... sets with relative standard deviation u < 0.2 are accepted. For example, the bead shown in Fig. 2(c) meets the motion and symmetry criterions; however, it displays a non-specific binding event at 30s. In short, our third criterion removes tethers with temporal inconsistency in their Brownian motion. ...
... sets with relative standard deviation u < 0.2 are accepted. For example, the bead shown in Fig. 2(c) meets the motion and symmetry criterions; however, it displays a non-specific binding event at 30s. In short, our third criterion removes tethers with temporal inconsistency in their Brownian motion. ...
Contig annotation tool CAT robustly classifies assembled
... questions in metagenomics (Handelsman, 2004) i.e. "Who is there?" and "What are they doing?". Read mapping considers each read as an independent observation, whose taxonomic origin and functional class can be estimated by identifying the closest match in a reference database, and tallying these ann ...
... questions in metagenomics (Handelsman, 2004) i.e. "Who is there?" and "What are they doing?". Read mapping considers each read as an independent observation, whose taxonomic origin and functional class can be estimated by identifying the closest match in a reference database, and tallying these ann ...
Nonenzymatic Sequence-Specific Cleavage of Single
... DNA to nucleotide resolution using chemically activated oligodeoxynucleotide methyl thioether hybridization probes (DNAMT). 5-[3-[ [3-(Methylthio)propionyl]amino]-trans-1propenylldeoxyuridine S’-triphosphate (MT-dUTP) was enzymatically incorporated into an oligonucleotide duplex by using the Klenow ...
... DNA to nucleotide resolution using chemically activated oligodeoxynucleotide methyl thioether hybridization probes (DNAMT). 5-[3-[ [3-(Methylthio)propionyl]amino]-trans-1propenylldeoxyuridine S’-triphosphate (MT-dUTP) was enzymatically incorporated into an oligonucleotide duplex by using the Klenow ...
DNA sequencing
DNA sequencing is the process of determining the precise order of nucleotides within a DNA molecule. It includes any method or technology that is used to determine the order of the four bases—adenine, guanine, cytosine, and thymine—in a strand of DNA. The advent of rapid DNA sequencing methods has greatly accelerated biological and medical research and discovery.Knowledge of DNA sequences has become indispensable for basic biological research, and in numerous applied fields such as medical diagnosis, biotechnology, forensic biology, virology and biological systematics. The rapid speed of sequencing attained with modern DNA sequencing technology has been instrumental in the sequencing of complete DNA sequences, or genomes of numerous types and species of life, including the human genome and other complete DNA sequences of many animal, plant, and microbial species.The first DNA sequences were obtained in the early 1970s by academic researchers using laborious methods based on two-dimensional chromatography. Following the development of fluorescence-based sequencing methods with a DNA sequencer, DNA sequencing has become easier and orders of magnitude faster.