Human polyomavirus JC control region variants in persistently
... isolated, thus giving a representative view on the JCV population present. A total of 91 clones was established from CNS tissue, and 227 clones were achieved from kidney tissue. Restriction cleavage of virus-specific inserts with the cloning enzymes BamHI}EcoRI revealed the presence of heterogeneous ...
... isolated, thus giving a representative view on the JCV population present. A total of 91 clones was established from CNS tissue, and 227 clones were achieved from kidney tissue. Restriction cleavage of virus-specific inserts with the cloning enzymes BamHI}EcoRI revealed the presence of heterogeneous ...
White biotechnology report RIVM
... available technique at the moment. It is mainly used for eukaryotic cells (fungi, yeasts), but prokaryotic organisms (bacteria, archaea) will probably follow. Still, the interviewed experts from industry were hesitant about the full‐fledged use of the technique in industrial microbi‐ ology, also b ...
... available technique at the moment. It is mainly used for eukaryotic cells (fungi, yeasts), but prokaryotic organisms (bacteria, archaea) will probably follow. Still, the interviewed experts from industry were hesitant about the full‐fledged use of the technique in industrial microbi‐ ology, also b ...
DNA Profile 911 - Bertino Forensics
... The different number of repeats are variations of the STRs or alleles of that STR. When trying to identify someone from their DNA, their physical appearance is not being examined. Instead, personal identification is based on their genes or alleles for different STRs. The more STRs being examined, t ...
... The different number of repeats are variations of the STRs or alleles of that STR. When trying to identify someone from their DNA, their physical appearance is not being examined. Instead, personal identification is based on their genes or alleles for different STRs. The more STRs being examined, t ...
SAY IT WITH DNA: PROTEIN SYNTHESIS WORKSHEET: Practice
... STEP 1: "Build" the mRNA molecule, matching the RNA nucleotides to the DNA nucleotides properly, letter by letter. (For purposes of simplicity, it will be assumed that this mRNA is bacterial; there are no introns to cut out!) STEP 2: Figure out the tRNA triplets (codons) that would fit the mRNA trip ...
... STEP 1: "Build" the mRNA molecule, matching the RNA nucleotides to the DNA nucleotides properly, letter by letter. (For purposes of simplicity, it will be assumed that this mRNA is bacterial; there are no introns to cut out!) STEP 2: Figure out the tRNA triplets (codons) that would fit the mRNA trip ...
A-2015A: Amplified Fragment Length
... has become a popular research tool for such diverse purposes as population genetics, molecular evolution, and breeding. Since the first protocol published by Keygene[1] to generate AFLP markers, many researchers have modified the protocol to suit their own detection systems and biological materials. ...
... has become a popular research tool for such diverse purposes as population genetics, molecular evolution, and breeding. Since the first protocol published by Keygene[1] to generate AFLP markers, many researchers have modified the protocol to suit their own detection systems and biological materials. ...
Tutorial - QIAGEN Bioinformatics
... Figure 19: Complete workflow for bisulfite sequencing in the graphical editor view. Notice that the workflow contains twice the tool for calling differential methylation to conduct the reciprocal analysis of methylation, so that both hyper- and hypo-methylated regions can be identified in one workfl ...
... Figure 19: Complete workflow for bisulfite sequencing in the graphical editor view. Notice that the workflow contains twice the tool for calling differential methylation to conduct the reciprocal analysis of methylation, so that both hyper- and hypo-methylated regions can be identified in one workfl ...
NucleoSpin 96 Flash Plasmid and Large-Construct DNA
... Use the 96-well Square-well Block for growing bacteria. Add 1.2–1.5 mL of selected medium (with appropriate antibiotic; for example 100 μg/mL ampicillin) to each well of the Square-well Block. To avoid cross-contamination due to spillage during incubation, do not exceed a total culture volume of 1.5 ...
... Use the 96-well Square-well Block for growing bacteria. Add 1.2–1.5 mL of selected medium (with appropriate antibiotic; for example 100 μg/mL ampicillin) to each well of the Square-well Block. To avoid cross-contamination due to spillage during incubation, do not exceed a total culture volume of 1.5 ...
Characterization of a novel DNA polymerase activity assay enabling
... tool with far-reaching applications such as, but not limited to, screening candidate-polymerase inhibitors in vitro, or detecting the presence any microbe (harboring active DNA polymerases) within a diverse range of sample types. If intended for these purposes, routine use of traditional polymerase ...
... tool with far-reaching applications such as, but not limited to, screening candidate-polymerase inhibitors in vitro, or detecting the presence any microbe (harboring active DNA polymerases) within a diverse range of sample types. If intended for these purposes, routine use of traditional polymerase ...
PPP Master Mix without MgCl2 - Top-Bio
... The Mix contains fluorescent DNA dye SYBR Green I, which after binding to double stranded (ds)DNA becomes strongly fluorescent with maximal excitation at 497 nm (blue light) and emission at 520 nm (green light). Because fluorescence of unbound SYBR Green I is very low, enhanced fluorescence during q ...
... The Mix contains fluorescent DNA dye SYBR Green I, which after binding to double stranded (ds)DNA becomes strongly fluorescent with maximal excitation at 497 nm (blue light) and emission at 520 nm (green light). Because fluorescence of unbound SYBR Green I is very low, enhanced fluorescence during q ...
DNA for Defence Lawyers
... These numbers can be computer coded and placed on the DNA database. When the same series of numbers comes up on another part of the database e.g. with a crime scene, suspect or convicted offender, a “match” is called and the two results further interpreted to see if the provisional match is justifie ...
... These numbers can be computer coded and placed on the DNA database. When the same series of numbers comes up on another part of the database e.g. with a crime scene, suspect or convicted offender, a “match” is called and the two results further interpreted to see if the provisional match is justifie ...
Structural basis for the inhibition of human alkyladenine Please share
... His-82, and Met-83 prior to residue 84. We refer to this truncated protein construct as ⌬79AAG, although the actual AAG protein sequence starts at Thr-84. It should be noted that the AAG protein from previous structural studies was also referred to as ⌬79AAG (9, 10). However, in those former studies ...
... His-82, and Met-83 prior to residue 84. We refer to this truncated protein construct as ⌬79AAG, although the actual AAG protein sequence starts at Thr-84. It should be noted that the AAG protein from previous structural studies was also referred to as ⌬79AAG (9, 10). However, in those former studies ...
Highly efficient nuclear DNA typing of the World War II skeletal
... DNA typing of bone and tooth samples has been successfully used in anthropological studies and forensic identification analysis (1,2). Nuclear DNA is the preferred genome of amplification for forensic purposes as it is individually specific and provides bi-parental kinship information (3). The succe ...
... DNA typing of bone and tooth samples has been successfully used in anthropological studies and forensic identification analysis (1,2). Nuclear DNA is the preferred genome of amplification for forensic purposes as it is individually specific and provides bi-parental kinship information (3). The succe ...
Using a Single-Nucleotide Polymorphism to Predict
... genes predict adverse responses to anti-depression drugs, including PROZAC® and Paxil®. In this experiment, a sample of human cells is obtained by saline mouthwash. DNA is extracted by boiling with Chelex resin, which binds contaminating metal ions. Polymerase chain reaction (PCR) is then used to am ...
... genes predict adverse responses to anti-depression drugs, including PROZAC® and Paxil®. In this experiment, a sample of human cells is obtained by saline mouthwash. DNA is extracted by boiling with Chelex resin, which binds contaminating metal ions. Polymerase chain reaction (PCR) is then used to am ...
The Role of DNA Structure and Dynamics in the
... BII conformation (CpG and GpT on the two strands). The protein E2/E2-BS system is of particular interest because the recognition clearly involves both direct and indirect readouts of DNA. The selection of the specific target by the protein may proceed along several non-exclusive strategies: (i) inte ...
... BII conformation (CpG and GpT on the two strands). The protein E2/E2-BS system is of particular interest because the recognition clearly involves both direct and indirect readouts of DNA. The selection of the specific target by the protein may proceed along several non-exclusive strategies: (i) inte ...
Molecular Computing Viability for Solving Computational Problems
... International Journal of Computer and Communication Engineering, Vol. 1, No. 3, September 2012 ...
... International Journal of Computer and Communication Engineering, Vol. 1, No. 3, September 2012 ...
STRUCTURE AND DIAGNOSTIC APPLICATIONS OF DNA
... • Nucleoside = Nitrogenous base + Sugar; • In DNA, sugar is Deoxyribose, • DNA contains Deoxy-Nucleosides: • Nitrogenous base + Deoxy-ribose; • Four types of Deoxy-Nucleosides in DNA: ...
... • Nucleoside = Nitrogenous base + Sugar; • In DNA, sugar is Deoxyribose, • DNA contains Deoxy-Nucleosides: • Nitrogenous base + Deoxy-ribose; • Four types of Deoxy-Nucleosides in DNA: ...
HLA-B27 real-time PCR using TaqMan
... Ser respectively instead of Cys (6,23,24). This mutation in transgenic rats leads to gut disease but markedly less susceptibility to arthritis (25). HLA-B*27:16, HLA-B*27:18, HLAB*27:23 and HLA-B*27:29 as well as HLA-B*27:12 all share Thr/Asn/Thr at residues 69/70/71 instead of Ala/Lys/Ala shared by ...
... Ser respectively instead of Cys (6,23,24). This mutation in transgenic rats leads to gut disease but markedly less susceptibility to arthritis (25). HLA-B*27:16, HLA-B*27:18, HLAB*27:23 and HLA-B*27:29 as well as HLA-B*27:12 all share Thr/Asn/Thr at residues 69/70/71 instead of Ala/Lys/Ala shared by ...
DNA Sequencing by Targeting 16S rRNA Gene for Novel Strain
... diversity of the genus has been well documented, and nearly every imaginable phenotype is represented in one species or another1. Bacillus sp. is widely distributed in the natural environments. They have unique ability to inhabit a variety of extreme and contaminated environments. Bacteria belonging ...
... diversity of the genus has been well documented, and nearly every imaginable phenotype is represented in one species or another1. Bacillus sp. is widely distributed in the natural environments. They have unique ability to inhabit a variety of extreme and contaminated environments. Bacteria belonging ...
Chapter 16: THE MOLECULAR BASIS OF INHERITANCE (DNA
... • Early in the twentieth century, scientists knew that the genes are on the chromosomes, but they did not know the composition of genes. The identification of the molecules of inheritance was a major challenge to biologists. • DNA and proteins were the candidates for the genetic material, but protei ...
... • Early in the twentieth century, scientists knew that the genes are on the chromosomes, but they did not know the composition of genes. The identification of the molecules of inheritance was a major challenge to biologists. • DNA and proteins were the candidates for the genetic material, but protei ...
Soliton-like base pair opening in a helicoidal DNA: An analogy with
... The B-form DNA double helix molecule is usually modeled by two parallel chains of nucleotides known as strands with linkage interms of dipole-dipole interaction along the strands and the two strands are coupled to each other through hydrogen bonds between the complementary bases [1]. Molecular exci ...
... The B-form DNA double helix molecule is usually modeled by two parallel chains of nucleotides known as strands with linkage interms of dipole-dipole interaction along the strands and the two strands are coupled to each other through hydrogen bonds between the complementary bases [1]. Molecular exci ...
Conformation and Rigidity of DNA Microcircles Containing waf1
... fraction of the linear ligamers formed circularize thermally in the absence of p53. Thus, the microcircles obtained are devoid of any structural alterations induced by p53 and the conformational properties observed must therefore be intrinsic to the p53 response-element DNA itself. We then employ Ma ...
... fraction of the linear ligamers formed circularize thermally in the absence of p53. Thus, the microcircles obtained are devoid of any structural alterations induced by p53 and the conformational properties observed must therefore be intrinsic to the p53 response-element DNA itself. We then employ Ma ...
pdf, 1.3 MB - DNA and Natural Algorithms Group
... the construction of synthetic replicators. Most of the DNA-based nanomachines that have been constructed (Yurke et al. 2000; Simmel and Yurke 2001; Li and Tan 2002; Simmel and Yurke 2002; Yan et al. 2002; Alberti and Mergny 2003; Feng et al. 2003) to date use the energy of hybridization to activate ...
... the construction of synthetic replicators. Most of the DNA-based nanomachines that have been constructed (Yurke et al. 2000; Simmel and Yurke 2001; Li and Tan 2002; Simmel and Yurke 2002; Yan et al. 2002; Alberti and Mergny 2003; Feng et al. 2003) to date use the energy of hybridization to activate ...
The Structure of Nucleic acids (DNA, RNA,Viral nucleic acids
... amounts of nuclear material that he obtained were insufficient for a thorough chemical analysis, but he did establish that it contained a novel substance that turned out not to be carbohydrate, lipid, or protein. This material, which consisted of DNA and protein, was slightly acidic and high in phos ...
... amounts of nuclear material that he obtained were insufficient for a thorough chemical analysis, but he did establish that it contained a novel substance that turned out not to be carbohydrate, lipid, or protein. This material, which consisted of DNA and protein, was slightly acidic and high in phos ...
DNA sequencing
DNA sequencing is the process of determining the precise order of nucleotides within a DNA molecule. It includes any method or technology that is used to determine the order of the four bases—adenine, guanine, cytosine, and thymine—in a strand of DNA. The advent of rapid DNA sequencing methods has greatly accelerated biological and medical research and discovery.Knowledge of DNA sequences has become indispensable for basic biological research, and in numerous applied fields such as medical diagnosis, biotechnology, forensic biology, virology and biological systematics. The rapid speed of sequencing attained with modern DNA sequencing technology has been instrumental in the sequencing of complete DNA sequences, or genomes of numerous types and species of life, including the human genome and other complete DNA sequences of many animal, plant, and microbial species.The first DNA sequences were obtained in the early 1970s by academic researchers using laborious methods based on two-dimensional chromatography. Following the development of fluorescence-based sequencing methods with a DNA sequencer, DNA sequencing has become easier and orders of magnitude faster.