PCR Amplification, Sequencing of 16S rRNA Genes with Universal
... RP73, Complete genome were genetically very close to our strain of Pseudomonas aeruginosa. Primer pairs database search tool revealed that 10 new sets of primer pairs. These sets were very helpful for further sequencing of 16S rRNA species specific gene region. ...
... RP73, Complete genome were genetically very close to our strain of Pseudomonas aeruginosa. Primer pairs database search tool revealed that 10 new sets of primer pairs. These sets were very helpful for further sequencing of 16S rRNA species specific gene region. ...
Agarose Gel Electrophoresis
... film (ASA 3000) offers ideal sensitivity, allowing as little as several nanograms of DNA to be detected on film after making adjustments of exposure time. Gel photography systems can be set up by purchasing and assembling the individual components (e.g., camera, film holder, filters, and UV transill ...
... film (ASA 3000) offers ideal sensitivity, allowing as little as several nanograms of DNA to be detected on film after making adjustments of exposure time. Gel photography systems can be set up by purchasing and assembling the individual components (e.g., camera, film holder, filters, and UV transill ...
Regulation of DNA Polymerase Exonucleolytic Proofreading Activity
... Canada. E-mail: [email protected] Genetics 148: 1551–1557 (April, 1998) ...
... Canada. E-mail: [email protected] Genetics 148: 1551–1557 (April, 1998) ...
Biosynthesis of the Antibiotic Nonribosomal Peptide Penicillin in
... biosynthesis of medical molecules and the potential for the discovery of novel therapeutics2, 3. The baker’s yeast Saccharomyces cerevisiae is the most well-characterised unicellular fungi and is also one of the main organisms used industrially for engineered biosynthesis4. Since the advent of synth ...
... biosynthesis of medical molecules and the potential for the discovery of novel therapeutics2, 3. The baker’s yeast Saccharomyces cerevisiae is the most well-characterised unicellular fungi and is also one of the main organisms used industrially for engineered biosynthesis4. Since the advent of synth ...
HotStarTaq® Plus DNA Polymerase and Master Mix and
... PCR enzymes from the indicated suppliers. Parallel reactions were performed following the suppliers’ recommendations, using 50 ng human genomic DNA. A 1.5 kb fragment of the human CFTR gene was amplified in 35 PCR cycles. Only QIAGEN hot-start enzymes provided highly specific amplification. M: marke ...
... PCR enzymes from the indicated suppliers. Parallel reactions were performed following the suppliers’ recommendations, using 50 ng human genomic DNA. A 1.5 kb fragment of the human CFTR gene was amplified in 35 PCR cycles. Only QIAGEN hot-start enzymes provided highly specific amplification. M: marke ...
GelRed™ Product Information Sheet
... acid dye designed to replace the highly toxic ethidium bromide (EtBr) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. GelRed and EtBr have virtually the same spectra (Figure 1), so you can directly replace EtBr with GelRed without changing your existing imaging system. In ...
... acid dye designed to replace the highly toxic ethidium bromide (EtBr) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. GelRed and EtBr have virtually the same spectra (Figure 1), so you can directly replace EtBr with GelRed without changing your existing imaging system. In ...
Effects of Salt Concentrations and Bending Energy on the Extent of
... DNA to condense spontaneously into a toroid (1–3). Monoand most divalent ions do not condense DNA; in fact, they are observed to raise the threshold concentration of polyvalent cations at which condensation occurs (4). Although such effects of counterions on DNA have been the subject of many experim ...
... DNA to condense spontaneously into a toroid (1–3). Monoand most divalent ions do not condense DNA; in fact, they are observed to raise the threshold concentration of polyvalent cations at which condensation occurs (4). Although such effects of counterions on DNA have been the subject of many experim ...
Dynamic DNA nanotechnology using strand displacement reactions
... enzyme-based molecular automaton that could perform a computation [56] where the outcome (the release of an antisense drug mimic) was dependent on the ab- ...
... enzyme-based molecular automaton that could perform a computation [56] where the outcome (the release of an antisense drug mimic) was dependent on the ab- ...
Directions for Use Taq DNA Polymerase, 500U
... Will UNG remove uracil from both ss- and dsDNA with the same efficiency? No, UNG removes uracil from ss-DNA at a rate of 200% compared to dsDNA. Will UNG remove uracil from RNA? Will it work with short oligos (e.g. 25-mer)? UNG does not act on uracil on the ribose sugar backbone. It will work on ura ...
... Will UNG remove uracil from both ss- and dsDNA with the same efficiency? No, UNG removes uracil from ss-DNA at a rate of 200% compared to dsDNA. Will UNG remove uracil from RNA? Will it work with short oligos (e.g. 25-mer)? UNG does not act on uracil on the ribose sugar backbone. It will work on ura ...
Agarose Gel Electrophoresis
... distance judged sufficient for separation of the DNA fragments. If ethidium bromide has been incorporated into the gel, the DNA can be visualized by placing the gel on a UV light source and can be photographed directly (see support protocol). Gels run in the absence of ethidium bromide can be staine ...
... distance judged sufficient for separation of the DNA fragments. If ethidium bromide has been incorporated into the gel, the DNA can be visualized by placing the gel on a UV light source and can be photographed directly (see support protocol). Gels run in the absence of ethidium bromide can be staine ...
Synthesis and isolation of a single-strand
... Classical in vitro selection of an aptamer starts with the preparation of an oligonucleotide library. LNA phosphoramidites are readily available [16, 17] and the de novo synthesis of an LNA-containing library is therefore reasonably straightforward. After partitioning of the library into ligand-bind ...
... Classical in vitro selection of an aptamer starts with the preparation of an oligonucleotide library. LNA phosphoramidites are readily available [16, 17] and the de novo synthesis of an LNA-containing library is therefore reasonably straightforward. After partitioning of the library into ligand-bind ...
PTC Polymorphism Lab Manual
... genes predict adverse responses to anti-depression drugs, including PROZAC® and Paxil®. In this experiment, a sample of human cells is obtained by saline mouthwash. DNA is extracted by boiling with Chelex resin, which binds contaminating metal ions. Polymerase chain reaction (PCR) is then used to am ...
... genes predict adverse responses to anti-depression drugs, including PROZAC® and Paxil®. In this experiment, a sample of human cells is obtained by saline mouthwash. DNA is extracted by boiling with Chelex resin, which binds contaminating metal ions. Polymerase chain reaction (PCR) is then used to am ...
Resolution of the type material of the Asian elephant, Elephas
... Ishida et al., 2013) and 73 E. maximus (unpubl. data). Three short regions that contained genus-diagnostic nucleotide sites were identified, and primers were designed with conserved sequences at 3′ ends, using the software PRIMER3 (Supporting Information Table S3.6; Rozen & Skaletsky, 2000). In anti ...
... Ishida et al., 2013) and 73 E. maximus (unpubl. data). Three short regions that contained genus-diagnostic nucleotide sites were identified, and primers were designed with conserved sequences at 3′ ends, using the software PRIMER3 (Supporting Information Table S3.6; Rozen & Skaletsky, 2000). In anti ...
Effect of non-histone proteins on thermal transition of chromatin and
... which the following main conclusions should be considered : 1) At tenperature higher than that of free DNA melting several transitions can be observed indicating a stabilizing effect. 2) A small but appreciable fraction of chromatin DNA melts at tenperatures well below that of infinite DNA helices o ...
... which the following main conclusions should be considered : 1) At tenperature higher than that of free DNA melting several transitions can be observed indicating a stabilizing effect. 2) A small but appreciable fraction of chromatin DNA melts at tenperatures well below that of infinite DNA helices o ...
Resolution of the type material of the Asian elephant, Elephas
... Ishida et al., 2013) and 73 E. maximus (unpubl. data). Three short regions that contained genus-diagnostic nucleotide sites were identified, and primers were designed with conserved sequences at 3′ ends, using the software PRIMER3 (Supporting Information Table S3.6; Rozen & Skaletsky, 2000). In anti ...
... Ishida et al., 2013) and 73 E. maximus (unpubl. data). Three short regions that contained genus-diagnostic nucleotide sites were identified, and primers were designed with conserved sequences at 3′ ends, using the software PRIMER3 (Supporting Information Table S3.6; Rozen & Skaletsky, 2000). In anti ...
Pfx50™ DNA Polymerase - Thermo Fisher Scientific
... Pfx50™ DNA Polymerase is a fusion enzyme consisting of recombinant DNA polymerase from the archaean Thermococcus zilligii fused to an accessory protein. The highly thermostable polymerase possesses a proofreading 3’→ 5’ exonuclease activity, while the accessory protein stabilizes primer-template com ...
... Pfx50™ DNA Polymerase is a fusion enzyme consisting of recombinant DNA polymerase from the archaean Thermococcus zilligii fused to an accessory protein. The highly thermostable polymerase possesses a proofreading 3’→ 5’ exonuclease activity, while the accessory protein stabilizes primer-template com ...
Detection of DNA molecules by SERS spectroscopy with silvered
... the classical spectra of the DNA molecules can be found by the SERS substrate mapping. Moreover, the prospects for the DNA detection by the SERS spectroscopy with lasers of 473, 633, and 785 nm wavelengths are very encouraging. The most promising result is in the detection of the DNA molecules at ve ...
... the classical spectra of the DNA molecules can be found by the SERS substrate mapping. Moreover, the prospects for the DNA detection by the SERS spectroscopy with lasers of 473, 633, and 785 nm wavelengths are very encouraging. The most promising result is in the detection of the DNA molecules at ve ...
16 System and a 10X Primer Pair Mix Stored in TE
... this change should not affect amplification results. This change is not necessary for the PowerPlex® Y System because TE-4 buffer is already used to prepare the PowerPlex® Y 10X Primer Pair Mix. We will continue to generate more real time stability data, and if the product shelf life is extended, th ...
... this change should not affect amplification results. This change is not necessary for the PowerPlex® Y System because TE-4 buffer is already used to prepare the PowerPlex® Y 10X Primer Pair Mix. We will continue to generate more real time stability data, and if the product shelf life is extended, th ...
Candidatus Liberibacter asiaticus - Southwest Florida Research and
... citrus, including Bacillus spp., Microbacterium spp., Sphingomonas spp., Hymenobacter spp., Rhodococcus spp., and Pantoea agglomerans. None of the bacteria reacted with the primers. For all the samples tested positive with HLB-65/HLB-66, the samples also were positive with OI1/OI2c, and vise versa ( ...
... citrus, including Bacillus spp., Microbacterium spp., Sphingomonas spp., Hymenobacter spp., Rhodococcus spp., and Pantoea agglomerans. None of the bacteria reacted with the primers. For all the samples tested positive with HLB-65/HLB-66, the samples also were positive with OI1/OI2c, and vise versa ( ...
Human Pif1 helicase is a G-quadruplex DNA
... syndrome RecQ family helicases, BLM (Bloom’s syndrome helicase) and WRN (Werner’s syndrome helicase), unwind DNA in the 3 → 5 direction and function in several aspects of DNA metabolism including transcription, replication and repair (reviewed in [22]). Both helicases also unwind G4 DNA in vitro a ...
... syndrome RecQ family helicases, BLM (Bloom’s syndrome helicase) and WRN (Werner’s syndrome helicase), unwind DNA in the 3 → 5 direction and function in several aspects of DNA metabolism including transcription, replication and repair (reviewed in [22]). Both helicases also unwind G4 DNA in vitro a ...
Infectivity analysis of two variable DNA B components of Mungbean
... (pGA1⋅9B22) (Jacob et al 2003) and KA27 (pGA1⋅5B27) DNA B components of MYMV-Vig, each containing two origins of replication as direct repeats, were constructed in a modified version of the binary vector pGA472 (An et al 1985). The partial dimer clone pGA1⋅5B27 was constructed in this study by cloni ...
... (pGA1⋅9B22) (Jacob et al 2003) and KA27 (pGA1⋅5B27) DNA B components of MYMV-Vig, each containing two origins of replication as direct repeats, were constructed in a modified version of the binary vector pGA472 (An et al 1985). The partial dimer clone pGA1⋅5B27 was constructed in this study by cloni ...
genotyping arabidopsis - STLCC.edu :: Users` Server
... For PCR, the only enzyme required is DNA polymerase. Magnesium chloride is added to the buffer because magnesium acts as a co-enzyme, fitting into the three dimensional structure of the protein to activate it. Too much magnesium can reduce the ability of the polymerase to stay attached to the templa ...
... For PCR, the only enzyme required is DNA polymerase. Magnesium chloride is added to the buffer because magnesium acts as a co-enzyme, fitting into the three dimensional structure of the protein to activate it. Too much magnesium can reduce the ability of the polymerase to stay attached to the templa ...
DNA sequencing
DNA sequencing is the process of determining the precise order of nucleotides within a DNA molecule. It includes any method or technology that is used to determine the order of the four bases—adenine, guanine, cytosine, and thymine—in a strand of DNA. The advent of rapid DNA sequencing methods has greatly accelerated biological and medical research and discovery.Knowledge of DNA sequences has become indispensable for basic biological research, and in numerous applied fields such as medical diagnosis, biotechnology, forensic biology, virology and biological systematics. The rapid speed of sequencing attained with modern DNA sequencing technology has been instrumental in the sequencing of complete DNA sequences, or genomes of numerous types and species of life, including the human genome and other complete DNA sequences of many animal, plant, and microbial species.The first DNA sequences were obtained in the early 1970s by academic researchers using laborious methods based on two-dimensional chromatography. Following the development of fluorescence-based sequencing methods with a DNA sequencer, DNA sequencing has become easier and orders of magnitude faster.