A Highly Efficient Method for the Construction of a Plasmid
... library. The new method was based on the addition of complementary single stranded oligomers to cDNA and vector as 5' overhangs and annealing of the 5' overhangs before ligation to increase ligation efficiency. To generate the long, complementary 5' overhangs to cDNA and vector DNA a common un phosp ...
... library. The new method was based on the addition of complementary single stranded oligomers to cDNA and vector as 5' overhangs and annealing of the 5' overhangs before ligation to increase ligation efficiency. To generate the long, complementary 5' overhangs to cDNA and vector DNA a common un phosp ...
RecA-mediated strand exchange traverses
... maintain the same reading frame during translation and, in the case of a single substitution, result in a change of just one codon while the rest of the codons remain unchanged. Except for rare cases of accidental creation of stop codons, the base substitutions are usually much less harmful than eve ...
... maintain the same reading frame during translation and, in the case of a single substitution, result in a change of just one codon while the rest of the codons remain unchanged. Except for rare cases of accidental creation of stop codons, the base substitutions are usually much less harmful than eve ...
voltammetric methods for determination of some anticancer drugs
... DNA is a structurally polymorphic macromolecule which, depending on the nucleotide sequence and environmental conditions, can adopt a variety of conformations. The double helical structre of DNA consists of two strands, each of them on the outside of the double helice and formed by altemating phosph ...
... DNA is a structurally polymorphic macromolecule which, depending on the nucleotide sequence and environmental conditions, can adopt a variety of conformations. The double helical structre of DNA consists of two strands, each of them on the outside of the double helice and formed by altemating phosph ...
Generalized Transduction by Phage P22 in Salmonella typhimurium. II. Mechanisms of Integration of Transducing DNA.
... in this experiment the density of the his+ particles can be calculated from the known densities of the phage (p = 1.506 g/cm3) and the pur+ transducing particles (p = l-511 g/om3). The average density of the his+ transducing particles in this gradient is calculated to be approximately l-512 g/cm3 (a ...
... in this experiment the density of the his+ particles can be calculated from the known densities of the phage (p = 1.506 g/cm3) and the pur+ transducing particles (p = l-511 g/om3). The average density of the his+ transducing particles in this gradient is calculated to be approximately l-512 g/cm3 (a ...
Detection of Agrobacterium vitis by polymerase chain reaction in
... has rapidly become a basic diagnostic and identification protocol in plant pathology as well (HENSON and FRENCH 1993, LOUWS et al. 1999). Early studies to identify Agrobacterium with PCR used pure bacterial cultures to determine the suitability of primers which were usually designed on ...
... has rapidly become a basic diagnostic and identification protocol in plant pathology as well (HENSON and FRENCH 1993, LOUWS et al. 1999). Early studies to identify Agrobacterium with PCR used pure bacterial cultures to determine the suitability of primers which were usually designed on ...
Identification and removal of colanic acid from plasmid DNA
... solvents such as ethanol (EtOH) and by polyethylene glycol. Because polysaccharides are anionic, they co-purify with DNA by anion-exchange chromatography. The commercially available laboratory scale purification methods, including Qiagen, use anionexchange chromatography for at least one major step ...
... solvents such as ethanol (EtOH) and by polyethylene glycol. Because polysaccharides are anionic, they co-purify with DNA by anion-exchange chromatography. The commercially available laboratory scale purification methods, including Qiagen, use anionexchange chromatography for at least one major step ...
The Preservation and Persistence of Human DNA in Soil during
... assistance in human identification efforts. Forensic taphonomy can be differentiated from Efremov’s formulation by the specificity of its goals, which include determination of postmortem interval (PMI; time between death and discovery) and assignment of cause/manner of death (Carter and Tibbet, 2008 ...
... assistance in human identification efforts. Forensic taphonomy can be differentiated from Efremov’s formulation by the specificity of its goals, which include determination of postmortem interval (PMI; time between death and discovery) and assignment of cause/manner of death (Carter and Tibbet, 2008 ...
Developmental Validation of the Quantifiler Real-Time
... DNA quantification methods are poorly suited to high-throughput or automated sample flow. We report the results of developmental validation of two new commercial kits that use “real-time” quantitative PCR to detect and quantify human nuclear DNA, designed for use with forensic samples. The Quantifil ...
... DNA quantification methods are poorly suited to high-throughput or automated sample flow. We report the results of developmental validation of two new commercial kits that use “real-time” quantitative PCR to detect and quantify human nuclear DNA, designed for use with forensic samples. The Quantifil ...
Array-based sequencing of filaggrin gene for
... The filaggrin gene (FLG) is essential for skin differentiation and epidermal barrier formation with links to skin diseases, however it has a highly repetitive nucleotide sequence containing very limited stretches of unique nucleotides for precise mapping to reference genomes. Sequencing strategies u ...
... The filaggrin gene (FLG) is essential for skin differentiation and epidermal barrier formation with links to skin diseases, however it has a highly repetitive nucleotide sequence containing very limited stretches of unique nucleotides for precise mapping to reference genomes. Sequencing strategies u ...
Deconstructing the Genome: DNA at High Resolution
... process of DNA replication, which occurs within growing colonies of cells. Developed during a technological revolution that began in the mid-1970s, recombinant DNA technology makes it possible to characterize DNA molecules directly, rather than indirectly through the phenotypes they produce. Genetic ...
... process of DNA replication, which occurs within growing colonies of cells. Developed during a technological revolution that began in the mid-1970s, recombinant DNA technology makes it possible to characterize DNA molecules directly, rather than indirectly through the phenotypes they produce. Genetic ...
Solving a Crime Using DNA Analysis and Chemistry
... This protocol will require running a polyacrylamide gel. You will need glass plates and the accompanying gel electrophoresis apparatus. You can purchase precast 6% polyacrylamide gels from GE Life Sciences and Invitrogen, but you will also need the appropriate gel electrophoresis apparatus for the p ...
... This protocol will require running a polyacrylamide gel. You will need glass plates and the accompanying gel electrophoresis apparatus. You can purchase precast 6% polyacrylamide gels from GE Life Sciences and Invitrogen, but you will also need the appropriate gel electrophoresis apparatus for the p ...
13 The Klenow fragment of DNA polymerase I Pol I actually appears
... Structural contributions to catalysis (nucleotidyl transfer) and fidelity- a stepwise mechanism of DNA synthesis The active site residues lie in palm domain. The primer terminus is believed to interact with tyrosine 776 (Y776). In addition, two essential aspartate residues, D705 and D882, also lie a ...
... Structural contributions to catalysis (nucleotidyl transfer) and fidelity- a stepwise mechanism of DNA synthesis The active site residues lie in palm domain. The primer terminus is believed to interact with tyrosine 776 (Y776). In addition, two essential aspartate residues, D705 and D882, also lie a ...
draft dna profiling bill 2007
... The Deoxyribose Nucleic Acid (DNA) analysis of body substances is a powerful technology that makes it possible to determine whether the source of origin of one body substance is identical to that of another, and further to establish the biological relationship, if any, between two individuals, livin ...
... The Deoxyribose Nucleic Acid (DNA) analysis of body substances is a powerful technology that makes it possible to determine whether the source of origin of one body substance is identical to that of another, and further to establish the biological relationship, if any, between two individuals, livin ...
Diversity of DNA methyltransferases that recognize asymmetric
... such an extensive dimer interface strengthens the possibility that M.TTHA0409 functions as a dimer. Gel filtration analysis yielded an apparent molecular weight of 82 kDa, suggesting that M.TTHA0409 exists in a dimeric form in the absence of DNA. Although its structure has been determined, the recog ...
... such an extensive dimer interface strengthens the possibility that M.TTHA0409 functions as a dimer. Gel filtration analysis yielded an apparent molecular weight of 82 kDa, suggesting that M.TTHA0409 exists in a dimeric form in the absence of DNA. Although its structure has been determined, the recog ...
Incorporation of reporter molecule
... analysis (5) and DNA sequencing (6,7). In addition, the catalytic repertoire of nucleic acids can potentially be expanded through incorporation of various functional groups linked to nucleotides followed by selection using the systematic evolution of ligands by exponential enrichment (SELEX) techniq ...
... analysis (5) and DNA sequencing (6,7). In addition, the catalytic repertoire of nucleic acids can potentially be expanded through incorporation of various functional groups linked to nucleotides followed by selection using the systematic evolution of ligands by exponential enrichment (SELEX) techniq ...
CSE8393 Introduction to Bioinformatics Lecture 16: DNA
... unbalanced vertices (each occurrence of an l − 1 fragment is shared by two l length fragments, except possibly for the first and last one). By adding an edge between two unbalanced vertices we can make the graph balanced. Then we can find an Euler cycle in the graph in linear time (since the graph i ...
... unbalanced vertices (each occurrence of an l − 1 fragment is shared by two l length fragments, except possibly for the first and last one). By adding an edge between two unbalanced vertices we can make the graph balanced. Then we can find an Euler cycle in the graph in linear time (since the graph i ...
The Polymerase Chain Reaction
... – The spots of DNA fragments can represent variations in gene sequences, mRNA complementary sequences, viral sequences, bacterial sequences, etc. ...
... – The spots of DNA fragments can represent variations in gene sequences, mRNA complementary sequences, viral sequences, bacterial sequences, etc. ...
A molecular method for assessing meiofauna diversity in marine
... Sediment samples were collected from East Beach at Fort DeSoto Park, St. Petersburg, FL, in 1.5mL microcentrifuge tubes and stored at -80°C. DNA was extracted from the sediment using an SDS-based extraction buffer and series of phenol, phenolchloroform, and chloroform extractions, and ethanol-precip ...
... Sediment samples were collected from East Beach at Fort DeSoto Park, St. Petersburg, FL, in 1.5mL microcentrifuge tubes and stored at -80°C. DNA was extracted from the sediment using an SDS-based extraction buffer and series of phenol, phenolchloroform, and chloroform extractions, and ethanol-precip ...
Complete Laboratory PDF
... another on a chromosome, the greater the chance that they will be inherited together as a unit (linked). Conversely, locations farther apart on the chromosome are more likely to be separated by chromosome recombination during meiosis. Thus, the frequency of recombination with previously mapped genes ...
... another on a chromosome, the greater the chance that they will be inherited together as a unit (linked). Conversely, locations farther apart on the chromosome are more likely to be separated by chromosome recombination during meiosis. Thus, the frequency of recombination with previously mapped genes ...
Reconstruction of the original mycoflora in pelleted feed by PCR
... after pelleting (subsamples in total n = 24) by cultural and molecular biological methods. A fungal-specific primer pair ITS1/ITS5.8R was used to amplify fungal DNA; PCR products were processed for the PCR-SSCP method. In the resulting acrylamide gel, more than 85% of DNA bands of ground feeds were ...
... after pelleting (subsamples in total n = 24) by cultural and molecular biological methods. A fungal-specific primer pair ITS1/ITS5.8R was used to amplify fungal DNA; PCR products were processed for the PCR-SSCP method. In the resulting acrylamide gel, more than 85% of DNA bands of ground feeds were ...
Novel Research Starts with GAPDH - Bio-Rad
... to amplify the region which contains one of the GAPDH gene sequences (second set of primers are nested inside the initial PCR product sequence) Biotechnology Explorer PCR primers are ...
... to amplify the region which contains one of the GAPDH gene sequences (second set of primers are nested inside the initial PCR product sequence) Biotechnology Explorer PCR primers are ...
Specific oligonucleotide primers for detection of endoglucanase
... EN1R, were chosen that were predicted to specifically amplify a 1,311 bpDNA fragment of the B. Subtilis, G. stearothermophilus and P. campinasensis. The Genbank database (NCBI) search for complimentary sequences revealed 100 % homology between the primers and the gene encodes endo-b-1,4-glucanase of ...
... EN1R, were chosen that were predicted to specifically amplify a 1,311 bpDNA fragment of the B. Subtilis, G. stearothermophilus and P. campinasensis. The Genbank database (NCBI) search for complimentary sequences revealed 100 % homology between the primers and the gene encodes endo-b-1,4-glucanase of ...
DNA sequencing
DNA sequencing is the process of determining the precise order of nucleotides within a DNA molecule. It includes any method or technology that is used to determine the order of the four bases—adenine, guanine, cytosine, and thymine—in a strand of DNA. The advent of rapid DNA sequencing methods has greatly accelerated biological and medical research and discovery.Knowledge of DNA sequences has become indispensable for basic biological research, and in numerous applied fields such as medical diagnosis, biotechnology, forensic biology, virology and biological systematics. The rapid speed of sequencing attained with modern DNA sequencing technology has been instrumental in the sequencing of complete DNA sequences, or genomes of numerous types and species of life, including the human genome and other complete DNA sequences of many animal, plant, and microbial species.The first DNA sequences were obtained in the early 1970s by academic researchers using laborious methods based on two-dimensional chromatography. Following the development of fluorescence-based sequencing methods with a DNA sequencer, DNA sequencing has become easier and orders of magnitude faster.