Charge Transport in DNA - Insights from
... of nucleobases on time scales larger than nanoseconds, dramatic enhancements in computational efficiency have to be made. In the final part of this work, one possible approach to this is developed - a parametrized model to simulate the CT in DNA. Not only will this model be able to describe the CT in ...
... of nucleobases on time scales larger than nanoseconds, dramatic enhancements in computational efficiency have to be made. In the final part of this work, one possible approach to this is developed - a parametrized model to simulate the CT in DNA. Not only will this model be able to describe the CT in ...
Distortion of quantitative genomic and expression
... increases of 1.08- and 1.14-fold in hybridization intensity in the presence of Cot-1 DNA (Table 1, reactions 3–6). These experiments suggested that the effects due to Cot-1 are related to the composition of repetitive sequences surrounding these sc intervals. An sc probe from HOXB1 (chr 17: 43 964 2 ...
... increases of 1.08- and 1.14-fold in hybridization intensity in the presence of Cot-1 DNA (Table 1, reactions 3–6). These experiments suggested that the effects due to Cot-1 are related to the composition of repetitive sequences surrounding these sc intervals. An sc probe from HOXB1 (chr 17: 43 964 2 ...
Unraveling DNA helicases
... catalyze the unwinding of energetically stable duplex DNA molecules using NTP hydrolysis as the source of energy [1,2]. They are important enzymatic tools for the cellular DNA machinery. They are known to play essential roles in nearly all aspects of nucleic acid metabolism, such as DNA replication, ...
... catalyze the unwinding of energetically stable duplex DNA molecules using NTP hydrolysis as the source of energy [1,2]. They are important enzymatic tools for the cellular DNA machinery. They are known to play essential roles in nearly all aspects of nucleic acid metabolism, such as DNA replication, ...
FLUORESCENT PROTEIN IN Escherichia coli
... paradigm to how infections were treated. Since then many other antiobiotics were developed, e.g. streptomycin against tuberculosis. This meant that the leading cause of death changed from being infections to being noninfectious diseases, i.e. cancer, stroke, etc. Meanwhile, extended use of antibioti ...
... paradigm to how infections were treated. Since then many other antiobiotics were developed, e.g. streptomycin against tuberculosis. This meant that the leading cause of death changed from being infections to being noninfectious diseases, i.e. cancer, stroke, etc. Meanwhile, extended use of antibioti ...
A Novel PCR Detection Method for Major Fish Pathogenic Bacteria
... API 20E system (Grisezet al., 1991), fluorescent antibody technique (Miyamoto and Eguchi, 1997), pulsed-field gel electrophoresis analysis (Skov et al., 1995), and DNA hybridization (Martinez-Picadoet al., 1996). However, these methods require considerable time and effort to identify V. anguillarum. ...
... API 20E system (Grisezet al., 1991), fluorescent antibody technique (Miyamoto and Eguchi, 1997), pulsed-field gel electrophoresis analysis (Skov et al., 1995), and DNA hybridization (Martinez-Picadoet al., 1996). However, these methods require considerable time and effort to identify V. anguillarum. ...
Bacteriophage A cloning system for the construction of
... endonucleases at the cloning site, subsequent treatment with phosphatase to ensure high insertion rates would be unnecessary. (iv) DNA inserts can be cloned in a known orientation relative to vector-encoded expression signals. The cloning vector XORF8 possesses a multiple cloning site (MCS) containi ...
... endonucleases at the cloning site, subsequent treatment with phosphatase to ensure high insertion rates would be unnecessary. (iv) DNA inserts can be cloned in a known orientation relative to vector-encoded expression signals. The cloning vector XORF8 possesses a multiple cloning site (MCS) containi ...
Complete
... physical model for explaining the complicated separation characteristics of DNA prisms is developed. The model assumes that DNA molecules in such devices act as damped springs, which change their lengths in response to the applied electric pulses, instead of a rigid chain, as assumed in prior works. ...
... physical model for explaining the complicated separation characteristics of DNA prisms is developed. The model assumes that DNA molecules in such devices act as damped springs, which change their lengths in response to the applied electric pulses, instead of a rigid chain, as assumed in prior works. ...
Drosophila - The Solomon H Snyder Department of Neuroscience
... followed by a sequencing reaction with another nested primer. The spPCR reaction remains highly efficient and specific due to the splinkerette design. Since the splinkerette oligonucleotide is not phosphorylated at its 59 sticky end, only the bottom 39 recessed strand of the splinkerette sticky end ...
... followed by a sequencing reaction with another nested primer. The spPCR reaction remains highly efficient and specific due to the splinkerette design. Since the splinkerette oligonucleotide is not phosphorylated at its 59 sticky end, only the bottom 39 recessed strand of the splinkerette sticky end ...
Illustrating Python via Bioinformatics Examples
... Department of Informatics, University of Oslo ...
... Department of Informatics, University of Oslo ...
Myriad--Ambry -- Final Version of Ambry Preliminary Injunction
... one side of the double helix and the second strand is on the other side. For any given gene, only one molecule strand (the “template strand”) is actually transcribed into mRNA and ultimately used to produce a protein, while the other strand is, for purposes of that gene, simply a space-filling molec ...
... one side of the double helix and the second strand is on the other side. For any given gene, only one molecule strand (the “template strand”) is actually transcribed into mRNA and ultimately used to produce a protein, while the other strand is, for purposes of that gene, simply a space-filling molec ...
GENtle, a free multi-purpose molecular biology tool
... seamlessly integrating diverse applications for every-day lab use in a single package. It was designed for easy and intuitive use, while providing many powerful functions. This C++ application runs on multiple platforms, is optimized for performance, and includes database interfaces for easy sequenc ...
... seamlessly integrating diverse applications for every-day lab use in a single package. It was designed for easy and intuitive use, while providing many powerful functions. This C++ application runs on multiple platforms, is optimized for performance, and includes database interfaces for easy sequenc ...
Potato Genome Sequencing Consortium: Final
... development of ‘massively parallel’ next generation sequencing (NGS) methods which have revolutionised genome analysis. Basically potato DNA is isolated by a simple procedure used for all plants, and then it is physically ‘sheared’ into small pieces using a sonication process. The small DNA fragment ...
... development of ‘massively parallel’ next generation sequencing (NGS) methods which have revolutionised genome analysis. Basically potato DNA is isolated by a simple procedure used for all plants, and then it is physically ‘sheared’ into small pieces using a sonication process. The small DNA fragment ...
Mechanistic Comparison of High-Fidelity and Error
... processive conditions. Herein our focus is on pre-steadystate kinetic constants, although the analogous steady-state constants are also used, as applied to the following terms: Catalytic efficiency is the pseudo-second-order rate constant for product formation with a given substrate, defined as kpol ...
... processive conditions. Herein our focus is on pre-steadystate kinetic constants, although the analogous steady-state constants are also used, as applied to the following terms: Catalytic efficiency is the pseudo-second-order rate constant for product formation with a given substrate, defined as kpol ...
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... other molecules, including the nucleic acid RNA. Transformation still occurred. Clearly, since those molecules had been destroyed, none of them could have been responsible for transformation. Avery’s team repeated the experiment one more time. This time, they used enzymes that would break down a dif ...
... other molecules, including the nucleic acid RNA. Transformation still occurred. Clearly, since those molecules had been destroyed, none of them could have been responsible for transformation. Avery’s team repeated the experiment one more time. This time, they used enzymes that would break down a dif ...
structure and mechanism of dna polymerases
... correct nucleotide from a pool of structurally similar nucleotides. The initial idea, proposed by Watson and Crick (1953a,b) based on the structure of DNA, was that selection could be determined by the A‐T and G‐C hydrogen bond–mediated base pairing. However, replicative polymerases have error rates ...
... correct nucleotide from a pool of structurally similar nucleotides. The initial idea, proposed by Watson and Crick (1953a,b) based on the structure of DNA, was that selection could be determined by the A‐T and G‐C hydrogen bond–mediated base pairing. However, replicative polymerases have error rates ...
Molecular events during translocation and proofreading extracted
... Figure 1. A cartoon representation of the main findings. DNA double helix is drawn as intertwined arcs as viewed along the major and minor grooves on one side of the DNA duplex. ␣ helices in the fingers domain are shown as cylinders. (A and C) viewed from a similar perspective show that the overall ...
... Figure 1. A cartoon representation of the main findings. DNA double helix is drawn as intertwined arcs as viewed along the major and minor grooves on one side of the DNA duplex. ␣ helices in the fingers domain are shown as cylinders. (A and C) viewed from a similar perspective show that the overall ...
Immobilization_Mecha..
... the gate region of the field effect transistor (FET) and the target strand free in the sample solution. The negative charge of the DNA backbone creates an electric field in the transistor which can be measured electronically.1−4 Over the past 15 years that FET based DNA sensors have been developed, nea ...
... the gate region of the field effect transistor (FET) and the target strand free in the sample solution. The negative charge of the DNA backbone creates an electric field in the transistor which can be measured electronically.1−4 Over the past 15 years that FET based DNA sensors have been developed, nea ...
Studies on Polynucleotides
... dCTP were used. Experiment 3 in Table I shows the results obtained on nearest neighbor analysis of the product obtained when [cG2P]dCTP alone was used. As expected, the radi0activit.y Ivas in dAp and dCp, the ratio found being 1.3. The result may again indicate that the incorporation of the terminal ...
... dCTP were used. Experiment 3 in Table I shows the results obtained on nearest neighbor analysis of the product obtained when [cG2P]dCTP alone was used. As expected, the radi0activit.y Ivas in dAp and dCp, the ratio found being 1.3. The result may again indicate that the incorporation of the terminal ...
Short Tandem Repeat Polymorphism and Cancer Risk: Influence of
... Genotype Versus Phenotype. The correlation between IGF-I genotype and phenotype was examined among all control subjects whose samples were analyzed by both methods. The results of the analysis are shown in Table 4. IGF-I phenotype in the circulation was correlated with IGF-I genotype in the long or ...
... Genotype Versus Phenotype. The correlation between IGF-I genotype and phenotype was examined among all control subjects whose samples were analyzed by both methods. The results of the analysis are shown in Table 4. IGF-I phenotype in the circulation was correlated with IGF-I genotype in the long or ...
Sequence Heterogeneities Among 16s
... different but closely related genera (enterobacteria) and for different species or strains within a single genus (Escherichia or Salmonella), and finally how similar are the ribosomal operons within a single organism (Escherichia coli). These sequences have been analyzed by neighbor-joining, maximum ...
... different but closely related genera (enterobacteria) and for different species or strains within a single genus (Escherichia or Salmonella), and finally how similar are the ribosomal operons within a single organism (Escherichia coli). These sequences have been analyzed by neighbor-joining, maximum ...
A simple centrifugation protocol leads to a 55-fold
... 1. DNA-based methods are increasingly used to study and monitor biodiversity and DNA (meta)barcoding is readily available for standardised assessments. However, (meta)barcoding suffers from bias introduced by PCR and the use of primers, which can lead to the loss of taxonomic groups. PCR-free techni ...
... 1. DNA-based methods are increasingly used to study and monitor biodiversity and DNA (meta)barcoding is readily available for standardised assessments. However, (meta)barcoding suffers from bias introduced by PCR and the use of primers, which can lead to the loss of taxonomic groups. PCR-free techni ...
Recombinant DNA technology and molecular cloning
... can be isolated and amplified. One method of isolation and amplification of a gene of interest is to clone the gene by inserting it into another DNA molecule that serves as a vehicle or vector that can be replicated in living cells. When these two DNAs of different origin are combined, the result is ...
... can be isolated and amplified. One method of isolation and amplification of a gene of interest is to clone the gene by inserting it into another DNA molecule that serves as a vehicle or vector that can be replicated in living cells. When these two DNAs of different origin are combined, the result is ...
Synthesis and Evaluation of Guanidino Phthalocyanines for G
... strongly suggesting a regulatory role in vivo. In vitro studies have already shown that these repeats can fold into triple helix-containing structures called H-DNA. Upon H-DNA formation, the DNA double helix within one-half of the H-palindrome denatures into two single strands, and one of these comp ...
... strongly suggesting a regulatory role in vivo. In vitro studies have already shown that these repeats can fold into triple helix-containing structures called H-DNA. Upon H-DNA formation, the DNA double helix within one-half of the H-palindrome denatures into two single strands, and one of these comp ...
DNA sequencing
DNA sequencing is the process of determining the precise order of nucleotides within a DNA molecule. It includes any method or technology that is used to determine the order of the four bases—adenine, guanine, cytosine, and thymine—in a strand of DNA. The advent of rapid DNA sequencing methods has greatly accelerated biological and medical research and discovery.Knowledge of DNA sequences has become indispensable for basic biological research, and in numerous applied fields such as medical diagnosis, biotechnology, forensic biology, virology and biological systematics. The rapid speed of sequencing attained with modern DNA sequencing technology has been instrumental in the sequencing of complete DNA sequences, or genomes of numerous types and species of life, including the human genome and other complete DNA sequences of many animal, plant, and microbial species.The first DNA sequences were obtained in the early 1970s by academic researchers using laborious methods based on two-dimensional chromatography. Following the development of fluorescence-based sequencing methods with a DNA sequencer, DNA sequencing has become easier and orders of magnitude faster.