Defining characteristics of Tn5 Transposase non
... amino acids of domain III binds supercoiled DNA nonspecifically (3) and that domain II can interact with linear non-specific DNA (4). The Tn3 Tnp binds non-specific linear restriction fragments (5,6). Finally, many Tnps, including Tn5 Tnp, can integrate their transposons into random DNA sequences (7 ...
... amino acids of domain III binds supercoiled DNA nonspecifically (3) and that domain II can interact with linear non-specific DNA (4). The Tn3 Tnp binds non-specific linear restriction fragments (5,6). Finally, many Tnps, including Tn5 Tnp, can integrate their transposons into random DNA sequences (7 ...
Fragaria multicipita - DigitalCommons@University of Nebraska
... F2n/R2. Following RFLP analysis using thirteen restriction enzymes including TaqI, the total of DNA fragment sizes in each RFLP pattern agreed with the 1.2 kbp size expected for 16S rDNA amplified in PCR primed by F2n/R2 and were the same as noted previously (Jomantiene et al. 1998 a) (Fig. 1, mF2/R ...
... F2n/R2. Following RFLP analysis using thirteen restriction enzymes including TaqI, the total of DNA fragment sizes in each RFLP pattern agreed with the 1.2 kbp size expected for 16S rDNA amplified in PCR primed by F2n/R2 and were the same as noted previously (Jomantiene et al. 1998 a) (Fig. 1, mF2/R ...
Assessing the Probative Value of DNA Evidence
... intended to be useful, and to be widely used, in all of the United Kingdom’s legal jurisdictions. It goes without saying that the laws of probability, unlike the laws of the land, are valid irrespective of geography. It would be artificial and sometimes misleading when describing criminal litigation ...
... intended to be useful, and to be widely used, in all of the United Kingdom’s legal jurisdictions. It goes without saying that the laws of probability, unlike the laws of the land, are valid irrespective of geography. It would be artificial and sometimes misleading when describing criminal litigation ...
2. Assessing the probative value of DNA evidence
... intended to be useful, and to be widely used, in all of the United Kingdom’s legal jurisdictions. It goes without saying that the laws of probability, unlike the laws of the land, are valid irrespective of geography. It would be artificial and sometimes misleading when describing criminal litigation ...
... intended to be useful, and to be widely used, in all of the United Kingdom’s legal jurisdictions. It goes without saying that the laws of probability, unlike the laws of the land, are valid irrespective of geography. It would be artificial and sometimes misleading when describing criminal litigation ...
Proof-of-principle rapid noninvasive prenatal diagnosis
... carriers in addition to the mother in family 2 (Figure 1). For family 1, the N370S carrier father was completely homozygous for the entire consensus N370S sequence. This precluded the use of the N370S haplotype for NIPD of his allele. Nonetheless, the familial mutation-linked haplotype of the father ...
... carriers in addition to the mother in family 2 (Figure 1). For family 1, the N370S carrier father was completely homozygous for the entire consensus N370S sequence. This precluded the use of the N370S haplotype for NIPD of his allele. Nonetheless, the familial mutation-linked haplotype of the father ...
Press release
... extent on the sample material itself (leaf and seed material, animal tissue, hair, etc.). sbeadex - high-quality DNA preparations The oKtopure utilises the sbeadexTM extraction chemistry developed by LGC, which is based on the use of magnetic particles. These particles can specifically bind nucleic ...
... extent on the sample material itself (leaf and seed material, animal tissue, hair, etc.). sbeadex - high-quality DNA preparations The oKtopure utilises the sbeadexTM extraction chemistry developed by LGC, which is based on the use of magnetic particles. These particles can specifically bind nucleic ...
Chapter 22: SV40 and Polyomavirus DNA Replication
... sequences: an A/T-rich stretch at the late border; a central G/C-rich palindrome, and a region referred to as the early palindrome at the early border (Fig. 2) (Parsons et al. 1990 and references therein). All polyomaviruses, including distantly related members like SV40 and PyV, display significant ...
... sequences: an A/T-rich stretch at the late border; a central G/C-rich palindrome, and a region referred to as the early palindrome at the early border (Fig. 2) (Parsons et al. 1990 and references therein). All polyomaviruses, including distantly related members like SV40 and PyV, display significant ...
Etude du régime alimentaire des carnivores par des techniques
... aim of my study was to develop a DNA-based universal method to study carnivores diet using environmental samples, and to apply this approach to several carnivore species. The main focus was on the diet of endangered and elusive wild felid species of Asia. Studying carnivores diet by characterizing t ...
... aim of my study was to develop a DNA-based universal method to study carnivores diet using environmental samples, and to apply this approach to several carnivore species. The main focus was on the diet of endangered and elusive wild felid species of Asia. Studying carnivores diet by characterizing t ...
Switching between polymerase and exonuclease sites in DNA
... To determine whether Pol ε is able to transfer the 3 terminus of the DNA from the polymerase site to the exonuclease site without dissociating from the DNA, we used heparin as a trap to capture any DNA polymerase molecules not bound to DNA. Titration experiments were done to ensure that the concent ...
... To determine whether Pol ε is able to transfer the 3 terminus of the DNA from the polymerase site to the exonuclease site without dissociating from the DNA, we used heparin as a trap to capture any DNA polymerase molecules not bound to DNA. Titration experiments were done to ensure that the concent ...
quantitation of male and female dna in mixed biological samples
... DNA can be isolated from nucleated cells present in the fluid. Once DNA has been isolated, polymorphic STR markers within the genome can be detected and analyzed following polymerase chain reaction (PCR) amplification (Butler, 2005). Multiplex PCRs are designed to amplify several loci simultaneously ...
... DNA can be isolated from nucleated cells present in the fluid. Once DNA has been isolated, polymorphic STR markers within the genome can be detected and analyzed following polymerase chain reaction (PCR) amplification (Butler, 2005). Multiplex PCRs are designed to amplify several loci simultaneously ...
Identity-by-descent filtering of exome sequence data for disease
... the ‘intersection’ approach. Although the intersection strategy has been useful in identifying Mendelian disease genes in the studies cited above, it is not always applicable. Many of the thousands of Mendelian disorders listed in OMIM, whose genetic basis is unknown, are often only clinically well ...
... the ‘intersection’ approach. Although the intersection strategy has been useful in identifying Mendelian disease genes in the studies cited above, it is not always applicable. Many of the thousands of Mendelian disorders listed in OMIM, whose genetic basis is unknown, are often only clinically well ...
The Role of DNA-PKcs and Artemis in Opening Viral DNA
... incubated with or without Exo III (10 U per g) at 37°C for 2 h in the appropriate reaction buffers, was separated on a 0.8% neutral agarose gel or a 1.0% alkaline agarose gel, and was subjected to Southern blot analysis as described above. The remaining portion of each DNA collection was digested w ...
... incubated with or without Exo III (10 U per g) at 37°C for 2 h in the appropriate reaction buffers, was separated on a 0.8% neutral agarose gel or a 1.0% alkaline agarose gel, and was subjected to Southern blot analysis as described above. The remaining portion of each DNA collection was digested w ...
Graph-based Methods for the Design of DNA - TUBdok
... evaluations of several DNA word sets from the literature is performed. 2. A new approach is developed for solving a minimal free energy (MFE) problem for the hybridization complex built by a pair of partially complementary DNA molecules. It introduces a concept of the hybridization graph to represen ...
... evaluations of several DNA word sets from the literature is performed. 2. A new approach is developed for solving a minimal free energy (MFE) problem for the hybridization complex built by a pair of partially complementary DNA molecules. It introduces a concept of the hybridization graph to represen ...
Analysis of clones carrying repeated DNA sequences in two YAC
... First, a set of overlapping cosmid clones has been assembled into 750 contigs covering 90-95% of the genome (Hauge et a/., 1991). Second, efforts are underway to construct overlapping YAC contigs for the five Arabidopsis chromosomes. The three YAC libraries commonly in use are the EG (Grill and Some ...
... First, a set of overlapping cosmid clones has been assembled into 750 contigs covering 90-95% of the genome (Hauge et a/., 1991). Second, efforts are underway to construct overlapping YAC contigs for the five Arabidopsis chromosomes. The three YAC libraries commonly in use are the EG (Grill and Some ...
Multiplex in-vitro Detection using SERS
... surface can allow the direct detection of a SERS signal from the constituent bases. 18, 61-63 Barhoumi and Halas investigated the SERS of thiolated single stranded and double stranded DNA oligomers bound to Au nanoshells.61 They found that thermal pretreatment of the DNA prior to adsorption onto the ...
... surface can allow the direct detection of a SERS signal from the constituent bases. 18, 61-63 Barhoumi and Halas investigated the SERS of thiolated single stranded and double stranded DNA oligomers bound to Au nanoshells.61 They found that thermal pretreatment of the DNA prior to adsorption onto the ...
2 SINGLE-MOLECULE DNA:PROTEIN INTERACTIONS - VU-dare
... limits of optical tweezers and what are the parameters that define these limits? In most cases, the performance of an optical tweezers instrument is limited by instrument drift and environmental noise. These factors can, to a large extent be suppressed by placing instruments in sound-isolated, tempe ...
... limits of optical tweezers and what are the parameters that define these limits? In most cases, the performance of an optical tweezers instrument is limited by instrument drift and environmental noise. These factors can, to a large extent be suppressed by placing instruments in sound-isolated, tempe ...
Binding of Hoechst with nucleic acids using fluorescence spectroscopy
... Full understanding of the molecular mechanisms of action of heterocyclic antibiotics at low concentrations on DNA and RNA can optimize the use of these compounds and their analogues as medical drugs. Hoechst dyes 33342 and 33258 are closely related bisbenzimides. Hoechst 33258 is 2’-(4-hydroxyphenyl ...
... Full understanding of the molecular mechanisms of action of heterocyclic antibiotics at low concentrations on DNA and RNA can optimize the use of these compounds and their analogues as medical drugs. Hoechst dyes 33342 and 33258 are closely related bisbenzimides. Hoechst 33258 is 2’-(4-hydroxyphenyl ...
Equilibrium Statistics of Channel-confined DNA
... This thesis concerns the statistical description of a very long molecule (a polymer) that is confined to a channel which is much narrower than the size of the molecule. The primary motivation is recent experiments where DNA molecules are inserted into channels with a width of about 100 nm. The resea ...
... This thesis concerns the statistical description of a very long molecule (a polymer) that is confined to a channel which is much narrower than the size of the molecule. The primary motivation is recent experiments where DNA molecules are inserted into channels with a width of about 100 nm. The resea ...
Analysis of Cross Sequence Similarities for Multiple - PolyU
... Without any compression, two bits per base are required for encoding four nucleotides. However, most general-purpose compression tools use more than two bits to encode a base. It is because these tools do not consider the special structures in a DNA sequence. Therefore, before discussing the similar ...
... Without any compression, two bits per base are required for encoding four nucleotides. However, most general-purpose compression tools use more than two bits to encode a base. It is because these tools do not consider the special structures in a DNA sequence. Therefore, before discussing the similar ...
Collaborative coupling between polymerase and helicase for
... separate activities at high forces or low dNTP concentration. We propose a collaborative model in which the helicase releases the fork regression pressure on the holoenzyme allowing it to adopt a processive polymerization conformation and the holoenzyme destabilizes the first few base pairs of the f ...
... separate activities at high forces or low dNTP concentration. We propose a collaborative model in which the helicase releases the fork regression pressure on the holoenzyme allowing it to adopt a processive polymerization conformation and the holoenzyme destabilizes the first few base pairs of the f ...
Extension Activity 1: Plasmid Mapping STUDENT MANU AL
... used in the DNA fingerprinting activity were constructed from the same pTZ18U plasmid parent but had different foreign fragments of DNA inserted into them. In the DNA fingerprinting exercise, only two restriction enzymes were used, but other enzymes could also have been used to cut these plasmids. T ...
... used in the DNA fingerprinting activity were constructed from the same pTZ18U plasmid parent but had different foreign fragments of DNA inserted into them. In the DNA fingerprinting exercise, only two restriction enzymes were used, but other enzymes could also have been used to cut these plasmids. T ...
DNA sequencing
DNA sequencing is the process of determining the precise order of nucleotides within a DNA molecule. It includes any method or technology that is used to determine the order of the four bases—adenine, guanine, cytosine, and thymine—in a strand of DNA. The advent of rapid DNA sequencing methods has greatly accelerated biological and medical research and discovery.Knowledge of DNA sequences has become indispensable for basic biological research, and in numerous applied fields such as medical diagnosis, biotechnology, forensic biology, virology and biological systematics. The rapid speed of sequencing attained with modern DNA sequencing technology has been instrumental in the sequencing of complete DNA sequences, or genomes of numerous types and species of life, including the human genome and other complete DNA sequences of many animal, plant, and microbial species.The first DNA sequences were obtained in the early 1970s by academic researchers using laborious methods based on two-dimensional chromatography. Following the development of fluorescence-based sequencing methods with a DNA sequencer, DNA sequencing has become easier and orders of magnitude faster.