DNA Base Sequence Homology in Rhizoctonia solani Kuihn: Inter
... (6), indicating genetic homogeneity among isolates within these groups. Hybridization between isolates of different AG was 30% or less (6,15). Ranges of DNA hybridization values varied for different AG, and lower levels of hybridization have confirmed lack of homogeneity among isolates within AG-1, ...
... (6), indicating genetic homogeneity among isolates within these groups. Hybridization between isolates of different AG was 30% or less (6,15). Ranges of DNA hybridization values varied for different AG, and lower levels of hybridization have confirmed lack of homogeneity among isolates within AG-1, ...
Short Tandem Repeat Polymorphism and Cancer Risk: Influence of
... Genotype Versus Phenotype. The correlation between IGF-I genotype and phenotype was examined among all control subjects whose samples were analyzed by both methods. The results of the analysis are shown in Table 4. IGF-I phenotype in the circulation was correlated with IGF-I genotype in the long or ...
... Genotype Versus Phenotype. The correlation between IGF-I genotype and phenotype was examined among all control subjects whose samples were analyzed by both methods. The results of the analysis are shown in Table 4. IGF-I phenotype in the circulation was correlated with IGF-I genotype in the long or ...
Nucleosides, Nucleotides,Nucleic Acids
... Nucleic acids first isolated in 1869 (Johann Miescher) Oswald Avery discovered (1945) that a substance which caused a change in the genetically transmitted characteristics of a bacterium was DNA. Scientists revised their opinion of the function of DNA and began to suspect it was the major functional ...
... Nucleic acids first isolated in 1869 (Johann Miescher) Oswald Avery discovered (1945) that a substance which caused a change in the genetically transmitted characteristics of a bacterium was DNA. Scientists revised their opinion of the function of DNA and began to suspect it was the major functional ...
The Art of DNA Strings: Sixteen Years of DNA Coding Theory
... To perform the DNA computation, DNA strands react with each other by Watson Crick base pairing and form perfect match. But in some situation, DNA strands may not form perfect base pairing and react in undesirable manner. One situation is formation of secondary structure in which first half strand of ...
... To perform the DNA computation, DNA strands react with each other by Watson Crick base pairing and form perfect match. But in some situation, DNA strands may not form perfect base pairing and react in undesirable manner. One situation is formation of secondary structure in which first half strand of ...
Gel immobilization of acrylamide-modified single
... automation [17], this method is comparatively expensive, although the streptavidin-coated magnetic beads can be reused [18]. For parallel pyrosequencing, Biotage AB (Uppsala, Sweden) developed the PyroMark MD that features an integrated vacuum prep workstation for the fully automated preparation of ...
... automation [17], this method is comparatively expensive, although the streptavidin-coated magnetic beads can be reused [18]. For parallel pyrosequencing, Biotage AB (Uppsala, Sweden) developed the PyroMark MD that features an integrated vacuum prep workstation for the fully automated preparation of ...
5 DNA Replication
... the entire chromosome would require almost 3 days. Yet, these bacteria are capable of dividing every 20 minutes. E. coli actually replicates its DNA at a rate of 1,000 nucleotides per second, with fewer than one in a billion errors. How is this extraordinarily accurate and rapid process accomplished ...
... the entire chromosome would require almost 3 days. Yet, these bacteria are capable of dividing every 20 minutes. E. coli actually replicates its DNA at a rate of 1,000 nucleotides per second, with fewer than one in a billion errors. How is this extraordinarily accurate and rapid process accomplished ...
Mutual Interactions of the Phosphate Groups in Locally Deformed
... (Borah et al. 1985; Nishimura et al. 1986; Sarma et al. 1986). It is also worth of being noticed that at 0.7 mol/l NaCl the backbones of both B- and A-DNA tend to overwind, presumably to counteract base stacking tendencies to unwind the double helices (Šponer and Kypr 1989). At high-salt, even the i ...
... (Borah et al. 1985; Nishimura et al. 1986; Sarma et al. 1986). It is also worth of being noticed that at 0.7 mol/l NaCl the backbones of both B- and A-DNA tend to overwind, presumably to counteract base stacking tendencies to unwind the double helices (Šponer and Kypr 1989). At high-salt, even the i ...
Week 2. DNA isolation and PCR
... of PCR and DNA replication with a student from another group. Then, I lead a class discussion that begins with having the students volunteer similarities and differences between PCR and DNA replication. I like to show the PCR video (https://www.youtube.com/watch?v=2KoLnIwoZKU) in the laboratory and ...
... of PCR and DNA replication with a student from another group. Then, I lead a class discussion that begins with having the students volunteer similarities and differences between PCR and DNA replication. I like to show the PCR video (https://www.youtube.com/watch?v=2KoLnIwoZKU) in the laboratory and ...
SNP Analysis of the PTC Gene Using PCR
... nucleotide is replaced by another nucleotide. For example when an A is replaced by a C, T or G (Figure 1). When such a mutation is present in at least 1% of the population it is know as a Single Nucleotide Polymorphism or SNP (pronounced “snip”.) A SNP can also occur when a single base pair has been ...
... nucleotide is replaced by another nucleotide. For example when an A is replaced by a C, T or G (Figure 1). When such a mutation is present in at least 1% of the population it is know as a Single Nucleotide Polymorphism or SNP (pronounced “snip”.) A SNP can also occur when a single base pair has been ...
e Study of RNA Polymerase Pausing by Optical Traps
... single molecule level elucidates properties of individual polymerases, such as pause probability and pause duration. From this data, we can investigate whether particular RNAP molecules tend to pause more frequently or longer than others. Recently, new techniques have been developed to study single ...
... single molecule level elucidates properties of individual polymerases, such as pause probability and pause duration. From this data, we can investigate whether particular RNAP molecules tend to pause more frequently or longer than others. Recently, new techniques have been developed to study single ...
CORPORATE RESPONSIBILITY AT DNA CORPORATE
... I have worked as a service advisor for consumer clients in Lahti for four years now. I had not given that much thought to responsibility from the company point of view, or how wide-spread its scope is, until I participated in the training last spring. While I may not be the most ecoconscious person ...
... I have worked as a service advisor for consumer clients in Lahti for four years now. I had not given that much thought to responsibility from the company point of view, or how wide-spread its scope is, until I participated in the training last spring. While I may not be the most ecoconscious person ...
Construction of plant BAC libraries This document
... etc. Seal wells with leftover melted 1% TBE agarose (50°C). Run the gel using the following parameters : volts/cm = 6.0, included angle = 120°, initial switch time = 1.0 sec, final switch time = 40.0 sec, ramping = linear, running time = 18 hours. ...
... etc. Seal wells with leftover melted 1% TBE agarose (50°C). Run the gel using the following parameters : volts/cm = 6.0, included angle = 120°, initial switch time = 1.0 sec, final switch time = 40.0 sec, ramping = linear, running time = 18 hours. ...
Nested PCR Assays for Detection of Monilinia fructicola in Stone
... DNA fragment amplified by microsatellite primer M13. The external and internal primer pairs EMfF + EMfR and IMfF + IMfR amplified a 571- and a 468bp fragment, respectively, from M. fructicola, but not from any other fungal species present in stone fruit orchards. The nested PCR primer pairs specific to ...
... DNA fragment amplified by microsatellite primer M13. The external and internal primer pairs EMfF + EMfR and IMfF + IMfR amplified a 571- and a 468bp fragment, respectively, from M. fructicola, but not from any other fungal species present in stone fruit orchards. The nested PCR primer pairs specific to ...
EPICENTRE Enzyme Catalog
... Polymerase is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,079,352, 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to US Patent No. 4,889,818. The purchase of this product includes a limited, non-transferable immunity f ...
... Polymerase is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,079,352, 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to US Patent No. 4,889,818. The purchase of this product includes a limited, non-transferable immunity f ...
3D DNA Crystals and Nanotechnology
... crystallography began to reveal the structural and functional details of proteins, but nucleic acid crystal structures were largely limited to relatively simple dinucleoside phosphate structures [3], primarily owing to the expense of longer oligonucleotides of discrete sequences needed for crystalli ...
... crystallography began to reveal the structural and functional details of proteins, but nucleic acid crystal structures were largely limited to relatively simple dinucleoside phosphate structures [3], primarily owing to the expense of longer oligonucleotides of discrete sequences needed for crystalli ...
Analysis of Guanine Oxidation Products in Double
... 2.2.1. Isolation and Identification of Oligomers Containing Ghox In Figure 5, four major peaks were detected at 19.6 and 20.0 min, with the peaks at 20.5 and 21.2 min having shoulder peaks. Two products (19.6 and 20.0 min) from Figure 5 were isolated and analyzed using electrospray ionization-mass s ...
... 2.2.1. Isolation and Identification of Oligomers Containing Ghox In Figure 5, four major peaks were detected at 19.6 and 20.0 min, with the peaks at 20.5 and 21.2 min having shoulder peaks. Two products (19.6 and 20.0 min) from Figure 5 were isolated and analyzed using electrospray ionization-mass s ...
IS1245 restriction fragment length polymorphism typing - HAL
... Cell lysis and DNA extraction should be performed as described previously (24). The choice of the restriction enzyme is strongly dependent on the range of sizes of DNA fragments obtained after cleavage of genomic DNA from M. avium strains. Several restriction enzymes provide a wide range of DNA frag ...
... Cell lysis and DNA extraction should be performed as described previously (24). The choice of the restriction enzyme is strongly dependent on the range of sizes of DNA fragments obtained after cleavage of genomic DNA from M. avium strains. Several restriction enzymes provide a wide range of DNA frag ...
PDF
... Attachment of DNA to both the magnetic bead and the surface of the flow cell is essential for single molecule studies. This is achieved by combining together an unmodified DNA with derivatized DNA handles. Such handles can be prepared in a variety of ways. Perhaps the easiest way involves PCR using ...
... Attachment of DNA to both the magnetic bead and the surface of the flow cell is essential for single molecule studies. This is achieved by combining together an unmodified DNA with derivatized DNA handles. Such handles can be prepared in a variety of ways. Perhaps the easiest way involves PCR using ...
Structural basis of PAM-dependent target DNA recognition by the
... C-terminal domain of Cas9 (Fig. 2c). The target-strand nucleotides complementary to the PAM are not recognized by major-groove interactions (Fig. 2b, c), rationalizing previous observations that Cas9-mediated DNA cleavage requires the 59-NGG-39 trinucleotide in the non-target strand, but not its tar ...
... C-terminal domain of Cas9 (Fig. 2c). The target-strand nucleotides complementary to the PAM are not recognized by major-groove interactions (Fig. 2b, c), rationalizing previous observations that Cas9-mediated DNA cleavage requires the 59-NGG-39 trinucleotide in the non-target strand, but not its tar ...
Development and Optimization of a DNA extraction
... repeated as needed in order to have the same sample volume to be introduced in the system as in the other cases, in order to be able to compare results. - Initial Protocol: The initial protocol was based on the extraction procedure performed in the commercial kits and it was tested in both microflui ...
... repeated as needed in order to have the same sample volume to be introduced in the system as in the other cases, in order to be able to compare results. - Initial Protocol: The initial protocol was based on the extraction procedure performed in the commercial kits and it was tested in both microflui ...
Amplification of 16S rRNA Genes from Frankia Strains in Root
... ranged from 0 to 5 CFU on LB agar and R2A agar per 104 hyphal clusters. Each hyphal cluster contained between 102 and 103 Frankia genomes, so the measurable proportion of contaminating bacterial genomes was quite low. Phenolic compounds present in actinorhizal nodule homogenates normally turn bright ...
... ranged from 0 to 5 CFU on LB agar and R2A agar per 104 hyphal clusters. Each hyphal cluster contained between 102 and 103 Frankia genomes, so the measurable proportion of contaminating bacterial genomes was quite low. Phenolic compounds present in actinorhizal nodule homogenates normally turn bright ...
Assessment of the mosaic structure in the
... sequencing and CGE analysis also revealed a bias of amplicons observed before (Fig. 2, lane 18) and after cloning (Fig. 2, lane 18a and 18h) which might be due to the fact that only highly abundant amplicons, present in the initial amplification derived from H. pylori HJM18MDA-DNA, were detectable b ...
... sequencing and CGE analysis also revealed a bias of amplicons observed before (Fig. 2, lane 18) and after cloning (Fig. 2, lane 18a and 18h) which might be due to the fact that only highly abundant amplicons, present in the initial amplification derived from H. pylori HJM18MDA-DNA, were detectable b ...
A Highly Efficient Method for the Construction of a Plasmid
... library. The new method was based on the addition of complementary single stranded oligomers to cDNA and vector as 5' overhangs and annealing of the 5' overhangs before ligation to increase ligation efficiency. To generate the long, complementary 5' overhangs to cDNA and vector DNA a common un phosp ...
... library. The new method was based on the addition of complementary single stranded oligomers to cDNA and vector as 5' overhangs and annealing of the 5' overhangs before ligation to increase ligation efficiency. To generate the long, complementary 5' overhangs to cDNA and vector DNA a common un phosp ...
The National DNA Database
... Act 1994, it has been overseen by a Management Board operated under a Memorandum of Understanding between the Forensic Science Service (FSS) and the Association of Chief Police Officers (ACPO). Recent developments, including the Home Office DNA Expansion Programme, the Review of the Forensic Science ...
... Act 1994, it has been overseen by a Management Board operated under a Memorandum of Understanding between the Forensic Science Service (FSS) and the Association of Chief Police Officers (ACPO). Recent developments, including the Home Office DNA Expansion Programme, the Review of the Forensic Science ...
DNA sequencing
DNA sequencing is the process of determining the precise order of nucleotides within a DNA molecule. It includes any method or technology that is used to determine the order of the four bases—adenine, guanine, cytosine, and thymine—in a strand of DNA. The advent of rapid DNA sequencing methods has greatly accelerated biological and medical research and discovery.Knowledge of DNA sequences has become indispensable for basic biological research, and in numerous applied fields such as medical diagnosis, biotechnology, forensic biology, virology and biological systematics. The rapid speed of sequencing attained with modern DNA sequencing technology has been instrumental in the sequencing of complete DNA sequences, or genomes of numerous types and species of life, including the human genome and other complete DNA sequences of many animal, plant, and microbial species.The first DNA sequences were obtained in the early 1970s by academic researchers using laborious methods based on two-dimensional chromatography. Following the development of fluorescence-based sequencing methods with a DNA sequencer, DNA sequencing has become easier and orders of magnitude faster.