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DNA & Protein Synthesis - Pottsgrove School District
DNA & Protein Synthesis - Pottsgrove School District

... • During DNA replication, the DNA molecule separates into 2 strands, and then produces 2 new complementary strands following the rules of base pairing. • Each strand of the double helix of DNA serves as a template, or model, for the new strand. ...
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DNA Nucleotides - Moore Public Schools

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additional file s4 - Springer Static Content Server

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... Breaks in a single strand of the DNA molecule are repaired using the same enzyme which are used in BER. • Double-Strand Breaks(DBSs) Two mechanism by which the cell can repair a complete breaks in a DNA molecule: ◦ One mechanism of protein that direct joining of the broken ends. This requires protei ...
DNA: The Genetic Material
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... The three-Dimensional Structure of DNA • The work of Rosalind Franklin involved X-ray crystallographic analysis of DNA • This involved bombarding the DNA molecules with beams of X-rays • Rosalind used DNA in the form of fibers in the laboratory of Maurice Wilkins • The work of Rosalind led to the d ...
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File - Gillam Biology

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Homologous recombination



Homologous recombination is a type of genetic recombination in which nucleotide sequences are exchanged between two similar or identical molecules of DNA. It is most widely used by cells to accurately repair harmful breaks that occur on both strands of DNA, known as double-strand breaks. Homologous recombination also produces new combinations of DNA sequences during meiosis, the process by which eukaryotes make gamete cells, like sperm and egg cells in animals. These new combinations of DNA represent genetic variation in offspring, which in turn enables populations to adapt during the course of evolution. Homologous recombination is also used in horizontal gene transfer to exchange genetic material between different strains and species of bacteria and viruses.Although homologous recombination varies widely among different organisms and cell types, most forms involve the same basic steps. After a double-strand break occurs, sections of DNA around the 5' ends of the break are cut away in a process called resection. In the strand invasion step that follows, an overhanging 3' end of the broken DNA molecule then ""invades"" a similar or identical DNA molecule that is not broken. After strand invasion, the further sequence of events may follow either of two main pathways discussed below (see Models); the DSBR (double-strand break repair) pathway or the SDSA (synthesis-dependent strand annealing) pathway. Homologous recombination that occurs during DNA repair tends to result in non-crossover products, in effect restoring the damaged DNA molecule as it existed before the double-strand break.Homologous recombination is conserved across all three domains of life as well as viruses, suggesting that it is a nearly universal biological mechanism. The discovery of genes for homologous recombination in protists—a diverse group of eukaryotic microorganisms—has been interpreted as evidence that meiosis emerged early in the evolution of eukaryotes. Since their dysfunction has been strongly associated with increased susceptibility to several types of cancer, the proteins that facilitate homologous recombination are topics of active research. Homologous recombination is also used in gene targeting, a technique for introducing genetic changes into target organisms. For their development of this technique, Mario Capecchi, Martin Evans and Oliver Smithies were awarded the 2007 Nobel Prize for Physiology or Medicine.
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