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Transcript
(DNA Ch 16)
Review Guide
1. Review and create notes for chapters 16, as well as associated replication, transcription and
translation
2. Understand the relationship between genes, proteins, amino acids, and chromosomes. Define each.
Also known as “The Central Dogma”
3. Know the structure of DNA. Be able to label a DNA or draw a DNA model on the test.
4. Describe the contributions of each of the following with regards to the discovery of DNA: Rosalind
Franklin, Watson & Crick, Chargaff, Wilson, Griffith (Avery) , Hershey-Chase
5. Describe the process of DNA replication. Know the players involved: DNA helicase, DNA polymerase,
replication fork, leading strand, lagging strand, Okazaki fragments, DNA ligase, complimentary strand,
base-pairing
6. Describe the process of transcription, including where it occurs and what components are necessary
for it to occur.
7. Compare and contrast DNA to RNA
8. Know the three regions associated with a gene: Promoter, coding region, terminator. Discuss the
roles of each within the lac operon.
9. Describe the process of translation, including where it occurs and what components are involved in it.
Pay particular attention to the role of tRNA and the anticodons. Also be able to use a codon chart to
determine the sequence of amino acids found in a gene.
10. Complete coloring sheet for more information and understanding of protein synthesis (see below)
11. Review website for animations of all of the above processes. Highly recommended in order to
solidify your understanding of these difficult biological concepts
12. Review topics in Biotechnology
Cloning
Recombinant DNA (Genetically Modified Organisms)
DNA fingerprinting (Gel electrophoresis)
13. Understand types of genetic mutations and their possible effects on phenotype (i.e point mutation,
frameshift, etc)
14. Review your old quizzes and the online practice quizzes.
DNA Coloring - Transcription & Translation
Transcription
RNA, Ribonucleic Acid is very similar to DNA. RNA normally exists as a single strand (and not
the double stranded double helix of DNA). It contains the same bases, adenine, guanine and
cytosine. However, there is no thymine found in RNA, instead there is a similar compound called
uracil.
Transcription is the process by which RNA is made from DNA. It occurs in the nucleus. Label
the box with the x in it near the nucleus with the word TRANSCRIPTION and proceed to color
the bases according to the key below
Thymine = orange
Cytosine = yellow
Adenine = dark green
Uracil = brown
Guanine = purple
Color the strand of DNA dark blue (D) and the strand of RNA light blue (R). Color the nuclear
membrane (E) gray.
Translation
Translation occurs in the cytoplasm, specifically on the ribosomes. The mRNA made in the
nucleus travels out to the ribosome to carry the message of the DNA. Here at the ribosome, that
message will be translated into an amino acid sequence. Color the ribosome light green (Y) and
note how the RNA strand threads through the ribsosome like a tape measure and the amino acids
are assembled. The RNA strand in the translation area should also be colored light blue, as it was
colored in the nucleus.
Label the box with the X in the translation area with the word TRANSLATION.
Important to the process of translation is another type of RNA called Transfer RNA (F) which
function to carry the amino acids to the site of protein synthesis on the ribosome. Color the tRNA
red.
A tRNA has two important areas. The anticodon, which matches the codon on the RNA strand.
Remember that codons are sets of three bases that code for a single amino acid. Make sure you
color the bases of the anticodon the same color as the bases on your DNA and RNA strand - they
are the same molecules!
At the top of the tRNA is the amino acids. There are twenty amino acids that can combine
together to form proteins of all kinds, these are the proteins that are used in life processes. When
you digest your food for instance, you are using enzymes that were originally proteins that were
assembled from amino acids. Each tRNA has a different amino acid which link together like box
cars on a train. Color all the amino acids (M) pink.
Questions:
1. How many different kinds of bases can be found on DNA _______
2. What base is found on RNA but not on DNA? _____________
3. How many bases are in a codon? ______ In an anticodon? ____________
4. How many amino acids are attached to a single transfer RNA? ________
5. Transcription occurs in the ____________________; translation occurs in the
________________________.
6. The process of making RNA from DNA is called ___________________ and it occurs in the
_________________________
7. The process of assembling a protein from RNA is called ____________________________
and it occurs in the _________________________
8. Describe two ways in which RNA differs from DNA:
_______________________________________________