The DNA repair helicase UvrD is essential for replication
... • RecG and RecA likely act independently of each other • RecG doesn't like free Mg • …because free cations freeze Holliday junction geometry? • RecG can work on fully duplex 3-stranded structures, but RecA cannot • (since RecA requires ssDNA for nucleation) • Why is the RecG reaction unidirectional? ...
... • RecG and RecA likely act independently of each other • RecG doesn't like free Mg • …because free cations freeze Holliday junction geometry? • RecG can work on fully duplex 3-stranded structures, but RecA cannot • (since RecA requires ssDNA for nucleation) • Why is the RecG reaction unidirectional? ...
Name Class Date DNA Replication Make Up #18 Lesson Objectives
... reconstruct the other half by the mechanism of base pairing. Because each strand can be used to make the other strand, the strands are said to be complementary. DNA copies itself through the process of replication: The two strands of the double helix unzip, forming replication forks. New bases are a ...
... reconstruct the other half by the mechanism of base pairing. Because each strand can be used to make the other strand, the strands are said to be complementary. DNA copies itself through the process of replication: The two strands of the double helix unzip, forming replication forks. New bases are a ...
DNAMocktst
... This type of RNA is a structural component of the ribosomes. Two terms for a sequence of 3 nucleotides that forms a code. Where is the anticodon located? In this process the mRNA is read to determine the protein structure. What signals the end of protein synthesis? ...
... This type of RNA is a structural component of the ribosomes. Two terms for a sequence of 3 nucleotides that forms a code. Where is the anticodon located? In this process the mRNA is read to determine the protein structure. What signals the end of protein synthesis? ...
Deoxyribonucleic Acid
... • 1. Avery took the “heat-killed ‘S’ and ‘R’” mixture and injected those bacteria with protein digesting enzymes. • 2. He took the same mixture and injected those bacteria with nucleic acid digesting enzymes. • He assumed that the one that was digested and did not infect would be the genetic materia ...
... • 1. Avery took the “heat-killed ‘S’ and ‘R’” mixture and injected those bacteria with protein digesting enzymes. • 2. He took the same mixture and injected those bacteria with nucleic acid digesting enzymes. • He assumed that the one that was digested and did not infect would be the genetic materia ...
RecA
... RecA protein functions: Repair of stalled replication fork double-strand break repair general recombination induction of the SOS response SOS mutagenesis ...
... RecA protein functions: Repair of stalled replication fork double-strand break repair general recombination induction of the SOS response SOS mutagenesis ...
The Molecular Basis of Heredity
... Base pairs are 0.34 nm apart. One complete turn of the helix requires 3.4 nm (10 bases/turn). ...
... Base pairs are 0.34 nm apart. One complete turn of the helix requires 3.4 nm (10 bases/turn). ...
Discovering DNA: Structure and Replication
... Nitrogen bases • The nitrogen base can either be a purine or a pyrimidine. • How many carbon rings does each have? – Purines have 2 – Pyrimidines have 1 ...
... Nitrogen bases • The nitrogen base can either be a purine or a pyrimidine. • How many carbon rings does each have? – Purines have 2 – Pyrimidines have 1 ...
DNA ‐ The Double Helix
... free to highlight or underline anything here, as they may help you answer the questions on page 5! ...
... free to highlight or underline anything here, as they may help you answer the questions on page 5! ...
DNA_and_Replication
... of DNA coiled around one another in a helix. The pictures also suggest that the nitrogenous bases are near the center of the ...
... of DNA coiled around one another in a helix. The pictures also suggest that the nitrogenous bases are near the center of the ...
DNA Sequencing
... They depend on the production of a mixture of oligonucleotides labeled either radioactively or fluorescein, with one common end and differing in length by a single nucleotide at the other end This mixture of oligonucleotides is separated by high resolution electrophoresis on polyacrilamide gels ...
... They depend on the production of a mixture of oligonucleotides labeled either radioactively or fluorescein, with one common end and differing in length by a single nucleotide at the other end This mixture of oligonucleotides is separated by high resolution electrophoresis on polyacrilamide gels ...
Name: Date: Period:___ Midterm Review: Study Guide # 3 TOPICS
... Topic # 6 – DNA/Chromosomes I am able to describe the structure of a DNA molecule: What part of the DNA molecule is in the shape of a pentagon? sugar ...
... Topic # 6 – DNA/Chromosomes I am able to describe the structure of a DNA molecule: What part of the DNA molecule is in the shape of a pentagon? sugar ...
Chapter 10 Practice Test
... 2. Homologous chromosomes are two chromosomes with identical DNA sequences. 3. During meiosis, chromosome number is reduced through three rounds of cell division. 4. In humans, the ability to roll one's tongue is a dominant trait. Therefore, a tongue roller can only have children who are also tongue ...
... 2. Homologous chromosomes are two chromosomes with identical DNA sequences. 3. During meiosis, chromosome number is reduced through three rounds of cell division. 4. In humans, the ability to roll one's tongue is a dominant trait. Therefore, a tongue roller can only have children who are also tongue ...
DNA Technology: The Future Is Now
... only began in April of 1995. In only 5 years, the English have created a database of nearly 1 million profiles of suspects and convicted offenders. Using this database, over 72,000 suspects have been linked to crimes and another 10,000 cases were linked to other similar crimes, even though the suspe ...
... only began in April of 1995. In only 5 years, the English have created a database of nearly 1 million profiles of suspects and convicted offenders. Using this database, over 72,000 suspects have been linked to crimes and another 10,000 cases were linked to other similar crimes, even though the suspe ...
DNA, RNA and the Genetic Code Worksheet
... The string of letters below represents one strand of DNA. First, transcribe the DNA strand into the mRNA sequence that would be produced from it. Then, identify the mRNA reading frame and draw vertical lines between the mRNA bases to separate the sequence into codons. Finally, use the genetic code t ...
... The string of letters below represents one strand of DNA. First, transcribe the DNA strand into the mRNA sequence that would be produced from it. Then, identify the mRNA reading frame and draw vertical lines between the mRNA bases to separate the sequence into codons. Finally, use the genetic code t ...
DNA - The Double Helix
... blueprints of a house tell the builders how to construct a house, the DNA "blueprint" tells the cell how to build the organism. Yet, how can a heart be so different from a brain if all the cells contain the same instructions? Although much work remains in genetics, it has become apparent that a cell ...
... blueprints of a house tell the builders how to construct a house, the DNA "blueprint" tells the cell how to build the organism. Yet, how can a heart be so different from a brain if all the cells contain the same instructions? Although much work remains in genetics, it has become apparent that a cell ...
Nucleic Acids & Protein Synthesis
... tightly together to form a chromatin. Chromatin: consists of DNA that is tightly packed around a protein called histone ...
... tightly together to form a chromatin. Chromatin: consists of DNA that is tightly packed around a protein called histone ...
Extracting DNA from Cells
... – Oil and water do not mix. If I have something that will dissolve in water and I shake it with oil, it will stay in the water layer. – If I put something in the water layer that is more soluble in oil, if I shake oil and water together it will transfer to the oil layer. I have extracted it from the ...
... – Oil and water do not mix. If I have something that will dissolve in water and I shake it with oil, it will stay in the water layer. – If I put something in the water layer that is more soluble in oil, if I shake oil and water together it will transfer to the oil layer. I have extracted it from the ...
Linkage
... (breaking H-Bonds) of the parental double helix. 2. Single-Strand Binding Proteins: proteins which attach and help keep the separated strands apart. 3. __________________: enzyme which relieves stress on the DNA molecule by allowing free rotation around a single strand. • Priming: 1._____ primers: b ...
... (breaking H-Bonds) of the parental double helix. 2. Single-Strand Binding Proteins: proteins which attach and help keep the separated strands apart. 3. __________________: enzyme which relieves stress on the DNA molecule by allowing free rotation around a single strand. • Priming: 1._____ primers: b ...
TABLE OF CONTENTS - Yale School of Medicine
... Today we are going to discuss the genetic material or DNA, deoxyribonucleic acid (write on the board and then have children say this). DNA is referred to as the blueprint for life. Show model of DNA and ask what kind of structure is this? Helix. The building blocks of DNA are deoxyribonucleotides. D ...
... Today we are going to discuss the genetic material or DNA, deoxyribonucleic acid (write on the board and then have children say this). DNA is referred to as the blueprint for life. Show model of DNA and ask what kind of structure is this? Helix. The building blocks of DNA are deoxyribonucleotides. D ...
UNIT 3 MOLECULAR GENETICS: REVIEW QUESTIONS Which
... 9. Describe the elongation and termination phases of translation. Use the following terms: P-site, A-site, elongation factor, GTP (cell energy), codon, tRNA, anticodon, amino acid, ribosome, stop/start codon, protein release factor, mRNA. ...
... 9. Describe the elongation and termination phases of translation. Use the following terms: P-site, A-site, elongation factor, GTP (cell energy), codon, tRNA, anticodon, amino acid, ribosome, stop/start codon, protein release factor, mRNA. ...
Deoxyribose nucleic acid
... • Structure was discovered in 1953 by James Watson and Francis Crick ...
... • Structure was discovered in 1953 by James Watson and Francis Crick ...
Homologous recombination
Homologous recombination is a type of genetic recombination in which nucleotide sequences are exchanged between two similar or identical molecules of DNA. It is most widely used by cells to accurately repair harmful breaks that occur on both strands of DNA, known as double-strand breaks. Homologous recombination also produces new combinations of DNA sequences during meiosis, the process by which eukaryotes make gamete cells, like sperm and egg cells in animals. These new combinations of DNA represent genetic variation in offspring, which in turn enables populations to adapt during the course of evolution. Homologous recombination is also used in horizontal gene transfer to exchange genetic material between different strains and species of bacteria and viruses.Although homologous recombination varies widely among different organisms and cell types, most forms involve the same basic steps. After a double-strand break occurs, sections of DNA around the 5' ends of the break are cut away in a process called resection. In the strand invasion step that follows, an overhanging 3' end of the broken DNA molecule then ""invades"" a similar or identical DNA molecule that is not broken. After strand invasion, the further sequence of events may follow either of two main pathways discussed below (see Models); the DSBR (double-strand break repair) pathway or the SDSA (synthesis-dependent strand annealing) pathway. Homologous recombination that occurs during DNA repair tends to result in non-crossover products, in effect restoring the damaged DNA molecule as it existed before the double-strand break.Homologous recombination is conserved across all three domains of life as well as viruses, suggesting that it is a nearly universal biological mechanism. The discovery of genes for homologous recombination in protists—a diverse group of eukaryotic microorganisms—has been interpreted as evidence that meiosis emerged early in the evolution of eukaryotes. Since their dysfunction has been strongly associated with increased susceptibility to several types of cancer, the proteins that facilitate homologous recombination are topics of active research. Homologous recombination is also used in gene targeting, a technique for introducing genetic changes into target organisms. For their development of this technique, Mario Capecchi, Martin Evans and Oliver Smithies were awarded the 2007 Nobel Prize for Physiology or Medicine.