DNA struct. and isol
... chemical and physical differences e.g. differential solubilities, precipitation, binding to columns and centrifugation ...
... chemical and physical differences e.g. differential solubilities, precipitation, binding to columns and centrifugation ...
Intro to DNA * Refresher Tasks
... Describe Watson and Crick’s model of the DNA molecule. Did Watson and Crick’s model account for the equal amounts of thymine and adenine in DNA? Explain. ...
... Describe Watson and Crick’s model of the DNA molecule. Did Watson and Crick’s model account for the equal amounts of thymine and adenine in DNA? Explain. ...
Lecture 19-Chap15
... • An endogenous gene can be replaced by a transfected gene using homologous recombination. • The occurrence of successful homologous recombination can be detected by using two selectable markers, one of which is incorporated with the integrated gene, the other of which is lost when recombination ...
... • An endogenous gene can be replaced by a transfected gene using homologous recombination. • The occurrence of successful homologous recombination can be detected by using two selectable markers, one of which is incorporated with the integrated gene, the other of which is lost when recombination ...
DNA
... •Genetic information is stored in the nucleus •Genetic information is essential so that each cell is identical (mitosis) ...
... •Genetic information is stored in the nucleus •Genetic information is essential so that each cell is identical (mitosis) ...
Prescott`s Microbiology, 9th Edition Chapter 16 –Mechanisms of
... If non homologous DNA was taken into the cell, it would not be able to align itself with a homologous region on the bacterial chromosome, and thus would not be integrated. The DNA could be degraded, but if not, could still only be passed to one daughter cell during cell division, and would thus be d ...
... If non homologous DNA was taken into the cell, it would not be able to align itself with a homologous region on the bacterial chromosome, and thus would not be integrated. The DNA could be degraded, but if not, could still only be passed to one daughter cell during cell division, and would thus be d ...
DNA: The Molecule of Heredity
... bases. But there are some major differences-• The sugar in RNA, is Ribose. • RNA is single stranded • the nitrogen bases consist of Uracil (U), Adenine (A), Guanine (G) and Cytosine (C). • Uracil and Adenine = Base Pair • Guanine and Cytosine = Base Pair ...
... bases. But there are some major differences-• The sugar in RNA, is Ribose. • RNA is single stranded • the nitrogen bases consist of Uracil (U), Adenine (A), Guanine (G) and Cytosine (C). • Uracil and Adenine = Base Pair • Guanine and Cytosine = Base Pair ...
ENVI 30 Environmental Issues
... Defective allele proteins with long glutamine strands Affects nervous system severe mental and physical deterioration death Typically appears later in life – Almost always before age 50 but almost never before age 20 ...
... Defective allele proteins with long glutamine strands Affects nervous system severe mental and physical deterioration death Typically appears later in life – Almost always before age 50 but almost never before age 20 ...
DNA Structure and Replication
... 3. A trait is any characteristic of a living thing. For example (name three traits): Answers will vary 4. DNA is found in the nucleus of a cell. 5. DNA makes up the chromosomes. 6. Humans have 23 pairs of chromosomes in the nucleus of each cell. 7. A gene is a small segment of DNA. 8. Genes have the ...
... 3. A trait is any characteristic of a living thing. For example (name three traits): Answers will vary 4. DNA is found in the nucleus of a cell. 5. DNA makes up the chromosomes. 6. Humans have 23 pairs of chromosomes in the nucleus of each cell. 7. A gene is a small segment of DNA. 8. Genes have the ...
DNA - Moore Public Schools
... • Point mutation – nitrogen base(A,T,C or G) in DNA sequence replaced with another. • This may not affect the protein being made. Because of the WOBBLE EFFECT. (3rd base) • Frame shift mutations – nitrogen base(A,T,C or G) is mistakenly inserted or deleted from the DNA sequence. • This almost always ...
... • Point mutation – nitrogen base(A,T,C or G) in DNA sequence replaced with another. • This may not affect the protein being made. Because of the WOBBLE EFFECT. (3rd base) • Frame shift mutations – nitrogen base(A,T,C or G) is mistakenly inserted or deleted from the DNA sequence. • This almost always ...
Lab Restriction Enzyme Analysis
... • 2. Become familiar with -Restriction enzymes -Electrophoresis -Analysis of DNA bands ...
... • 2. Become familiar with -Restriction enzymes -Electrophoresis -Analysis of DNA bands ...
DNA_NOTES
... Polarity of DNA • The polarity of a DNA molecule is opposite; • the ____ end of one strand matches up to the _____ end of the other strand. Thus, the strands are said to be "_____________________" ...
... Polarity of DNA • The polarity of a DNA molecule is opposite; • the ____ end of one strand matches up to the _____ end of the other strand. Thus, the strands are said to be "_____________________" ...
Ch12 DNA
... Don’t Studied bacteriophages (viruses) “bacteria eaters” copy made of DNA/RNA and protein coat Don’t Bacteriophages inject DNA into bacteria, the viral copy genes act to produce many new bacteriophages and burst of out cell Conclusion: using radioactive markers, genetic material of bacteriophage was ...
... Don’t Studied bacteriophages (viruses) “bacteria eaters” copy made of DNA/RNA and protein coat Don’t Bacteriophages inject DNA into bacteria, the viral copy genes act to produce many new bacteriophages and burst of out cell Conclusion: using radioactive markers, genetic material of bacteriophage was ...
Honors DNA Protein Synthesis Study Guide
... 2. When does DNA Replication occur? ______________________________________________________________________________ ___________ 3. Fill in the table below with the enzymes we discussed that are involved in DNA Replication (in order) and their functions: Step Enzyme Function(s) ...
... 2. When does DNA Replication occur? ______________________________________________________________________________ ___________ 3. Fill in the table below with the enzymes we discussed that are involved in DNA Replication (in order) and their functions: Step Enzyme Function(s) ...
Bio 313 Exam 1 Review - Iowa State University
... Please note that this exam review does not cover everything that will appear on the test and not everything on this review will end up on the test. I have not seen the test, so I am just using my knowledge to best prepare you for what will likely be on the test. ...
... Please note that this exam review does not cover everything that will appear on the test and not everything on this review will end up on the test. I have not seen the test, so I am just using my knowledge to best prepare you for what will likely be on the test. ...
Ch. 10 DNA, RNA, and Protein Synthesis Study Guide
... 6.) Two Nitrogenous bases are the _______________ , which have a double carbon ring and the ______________________, which have a single carbon ring. 7.) What are the two Purines and the two Pyrimidines and which ones are involved in base pairing? ...
... 6.) Two Nitrogenous bases are the _______________ , which have a double carbon ring and the ______________________, which have a single carbon ring. 7.) What are the two Purines and the two Pyrimidines and which ones are involved in base pairing? ...
DNA Fingerprinting of Bacterial Communities
... microorganisms can be easily cultured – Culture-based studies are skewed ...
... microorganisms can be easily cultured – Culture-based studies are skewed ...
Bacterial Genetics
... • Reproduce asexually • However, bacterial reproduction from one cell to another by: Transformation Conjugation Transduction ...
... • Reproduce asexually • However, bacterial reproduction from one cell to another by: Transformation Conjugation Transduction ...
Chromosomes and DNA Replication
... Histones have changed very little during evolution During most of cell cycle fibers are dispersed - can’t see chromosomes During mitosis, fibers are drawn together, forming tightly packed chromosomes you can see with a microscope ...
... Histones have changed very little during evolution During most of cell cycle fibers are dispersed - can’t see chromosomes During mitosis, fibers are drawn together, forming tightly packed chromosomes you can see with a microscope ...
DNA Modeling
... don’t fit, figure out a way to make them fit. 4. Show DNA Replication: a. separate the left and right sides leaving a space of about 6-8 inches between. b. use the remaining nucleotides to complete the molecule using the left side as the base to match with. c. build a second DNA model by adding new ...
... don’t fit, figure out a way to make them fit. 4. Show DNA Replication: a. separate the left and right sides leaving a space of about 6-8 inches between. b. use the remaining nucleotides to complete the molecule using the left side as the base to match with. c. build a second DNA model by adding new ...
lecture 7
... recognize and remove specific damaged or inappropriate bases, forming AP sites. These are then cleaved by an AP endonuclease. The resulting single-strand break can then be processed by either short-patch (where a single nucleotide is replaced) or long-patch BER (where 2-10 new nucleotides are synthe ...
... recognize and remove specific damaged or inappropriate bases, forming AP sites. These are then cleaved by an AP endonuclease. The resulting single-strand break can then be processed by either short-patch (where a single nucleotide is replaced) or long-patch BER (where 2-10 new nucleotides are synthe ...
The Structure of DNA Webquest
... 1) Read “The Problem" Q.1. Why was Oswald Avery’s experiment so important? _________________________________________________________________ _________________________________________________________________ _________________________________________________________________ 2) Read "Players" to get a ...
... 1) Read “The Problem" Q.1. Why was Oswald Avery’s experiment so important? _________________________________________________________________ _________________________________________________________________ _________________________________________________________________ 2) Read "Players" to get a ...
DNA Who`s Who
... 27. The organelle responsible for constructing proteins is the ____________________________. 28. Coding portions of mRNA that are kept and expressed are __________________________. 29. The three letter sequence in mRNA that codes for an amino acid is called a _____________________. 30. The process o ...
... 27. The organelle responsible for constructing proteins is the ____________________________. 28. Coding portions of mRNA that are kept and expressed are __________________________. 29. The three letter sequence in mRNA that codes for an amino acid is called a _____________________. 30. The process o ...
DNA and RNA study guide Answer Key
... 7. Before a cell divides, it must duplicate its own DNA in a process known as… DNA Replication 8. The genetic code in DNA depends upon the order or sequence of… Nitrogenous Bases 9. If one strand of DNA has the nitrogenous base sequence ATCGT, the sequence of bases on the COMPLEMENTARY STRAND of DNA ...
... 7. Before a cell divides, it must duplicate its own DNA in a process known as… DNA Replication 8. The genetic code in DNA depends upon the order or sequence of… Nitrogenous Bases 9. If one strand of DNA has the nitrogenous base sequence ATCGT, the sequence of bases on the COMPLEMENTARY STRAND of DNA ...
Name - Humble ISD
... Matching- There may be more than 1 correct answer for each question; however each choice is only used 1 time! ...
... Matching- There may be more than 1 correct answer for each question; however each choice is only used 1 time! ...
Heredity Unit 2 - Milton
... 5. What atom is found in structure A that is NOT found in structure B? PROTEIN COAT Sulfur ...
... 5. What atom is found in structure A that is NOT found in structure B? PROTEIN COAT Sulfur ...
Homologous recombination
Homologous recombination is a type of genetic recombination in which nucleotide sequences are exchanged between two similar or identical molecules of DNA. It is most widely used by cells to accurately repair harmful breaks that occur on both strands of DNA, known as double-strand breaks. Homologous recombination also produces new combinations of DNA sequences during meiosis, the process by which eukaryotes make gamete cells, like sperm and egg cells in animals. These new combinations of DNA represent genetic variation in offspring, which in turn enables populations to adapt during the course of evolution. Homologous recombination is also used in horizontal gene transfer to exchange genetic material between different strains and species of bacteria and viruses.Although homologous recombination varies widely among different organisms and cell types, most forms involve the same basic steps. After a double-strand break occurs, sections of DNA around the 5' ends of the break are cut away in a process called resection. In the strand invasion step that follows, an overhanging 3' end of the broken DNA molecule then ""invades"" a similar or identical DNA molecule that is not broken. After strand invasion, the further sequence of events may follow either of two main pathways discussed below (see Models); the DSBR (double-strand break repair) pathway or the SDSA (synthesis-dependent strand annealing) pathway. Homologous recombination that occurs during DNA repair tends to result in non-crossover products, in effect restoring the damaged DNA molecule as it existed before the double-strand break.Homologous recombination is conserved across all three domains of life as well as viruses, suggesting that it is a nearly universal biological mechanism. The discovery of genes for homologous recombination in protists—a diverse group of eukaryotic microorganisms—has been interpreted as evidence that meiosis emerged early in the evolution of eukaryotes. Since their dysfunction has been strongly associated with increased susceptibility to several types of cancer, the proteins that facilitate homologous recombination are topics of active research. Homologous recombination is also used in gene targeting, a technique for introducing genetic changes into target organisms. For their development of this technique, Mario Capecchi, Martin Evans and Oliver Smithies were awarded the 2007 Nobel Prize for Physiology or Medicine.