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DNA structure/genome/plasmid
DNA structure/genome/plasmid

... Plasmid- extra chromosomal DNA, found naturally mainly in bacteria ...
BioDynami 1 kb plus DNA ladder, ready-to-load
BioDynami 1 kb plus DNA ladder, ready-to-load

... www.BioDynami.com 601 Genome way, Huntsville, AL 35806 ...
DNA and Protein Synthesis
DNA and Protein Synthesis

... deleted from amino acid. This changes all the amino acids in the protein.  Compare Point and Frameshift mutations on the top of left page. Use fig 11.11 p298 for reference.  Chromosomal mutations: where parts of a chromosome are broken and lost or rejoined incorrectly. video  Compare the 4 Chromo ...
Slide 1
Slide 1

... 8-bp target site ...
DNA Replication - Blue Valley Schools
DNA Replication - Blue Valley Schools

... • Base pairs are formed by the hydrogen bonding of A with T and G with C. • This pattern is constant for all organisms. • The sequence of bases in a nucleotide strand is different from one species to the next. ...
DNA Paper Model Lab 7R 2016
DNA Paper Model Lab 7R 2016

... up into a bundle. This packaged form of the DNA is called a chromosome. DNA is made of chemical building blocks called nucleotides. The nucleotides are made up of three parts: a phosphate group, a deoxyribose/sugar group and one of four types of nitrogen bases (A,T,G and C). The nitrogen bases are t ...
Biology: DNA Review Packet
Biology: DNA Review Packet

... 1. Label EVERY sugar (S), phosphate (P), and nitrogen base (A, T, C, G) in the diagram below. ...
GEL ELECTROPHORESIS
GEL ELECTROPHORESIS

... What is gel electrophoresis? -It is a procedure used to separate and analyze DNA fragments at one ends of a porous gel and by applying an electrical voltage to the gel. (See Bio book p. 404). ...
Document
Document

... • Coupling (cis configuration): Wild type alleles are found on one chromosome; mutant alleles are found on the other chromosome. ...
DNA - Veritas Science
DNA - Veritas Science

... synthesize DNA using radioactive phosphorous. 2. The radioactive phosphorous "labeled" the DNA. 3. They forced another group of phages to synthesize protein using radioactive sulfur. 4. The radioactive sulfur "labeled" the protein. 5. Bacteria infected by phages containing radioactive ...
Wks #10 Answers
Wks #10 Answers

... The proteins were a much more diverse and complex group of molecules which exhibited a lot of molecular heterogeneity and thus were excellent candidates. e. The uptake of exogenous/external DNA material, most often by bacterial cells is? ___transformation_____ f. ...
Complete the definitions (4 pts each)
Complete the definitions (4 pts each)

... 40. What danger is avoided when using genetic engineering techniques to make vaccines as compared to the conventional method used to make the original polo vaccine? When making traditional vaccines scientist weaken or kill a harmful virus or bacteria and inject it into the body so that the person’s ...
DNA Replication
DNA Replication

... Conducted a series of experiments with bacteriophages – virus that infects bacteria- which identified DNA, not protein, to be the genetic material of cells. ...
IB Molecular Biology Review Game
IB Molecular Biology Review Game

... o Phosphate, deoxyribose sugar, and a nitrogenous base  What are the names of the 4 nitrogenous bases found in DNA? o Adenine, Thymine, Cytosine, Guanine  In what way are the 2 halves of a DNA strand oriented toward each other? o Anti-parallel  What kind of bond connects the phosphate of one nucl ...
Document
Document

... There are four nitrogen bases making up four different nucleotides. ...
DNA:PROTEIN SYNTHESIS REVIEW
DNA:PROTEIN SYNTHESIS REVIEW

...  Organisms are dependent on the correct _________ of genetic information from parents to ________  DNA’s presence has been known since 1874, but its function hasn’t always been clear.  Replicate--to make an accurate and exact _______ of the DNA ...
Science - Biology Source:http://www.ducksters.com/science/biology
Science - Biology Source:http://www.ducksters.com/science/biology

... Although DNA looks like very thin long strings under a microscope, it turns out that DNA has a specific shape. This shape is called a double helix. On the outside of the double helix is the backbone which holds the DNA together. There are two sets of backbones that twist together. Between the backbo ...
DNA is the Genetic Material
DNA is the Genetic Material

... Figure 7-2. The presence of heat-killed S cells transforms live R cells into live S cells. Notes: First, they did not need mice as the R and S phenotype was enough. Second, you will do the same in the lab (transformation), but using a plasmid containing an ...
Station 7 - Components of DNA
Station 7 - Components of DNA

... • How does the structure of DNA make it possible for traits to be passed on from one generation to another? The nucleotide bases that are paired on a DNA strand are complementary to each other. When the DNA molecule unzips and unwinds during DNA synthesis, the exposed strand of bases serves as a tem ...
cDNA Libraries
cDNA Libraries

... chromosomal DNA from a particular species. Complete genomic library: Entire genome is represented in at least one clone. cDNA Libraries: cDNAs = DNA copies of RNA molecules. cDNA libraries: Each clone contains DNA copy of an individual mRNA. Very useful for studying just the part of a gene that is p ...
DNA Quiz – Tuesday, November 5
DNA Quiz – Tuesday, November 5

... Compare and contrast DNA and RNA molecules. Outline the steps of DNA replication. Determine the complementary DNA sequence following DNA replication: A T G C G A T C G A A G ...
No Slide Title
No Slide Title

... Repairing double-strand breaks • Double-strand breaks can be repaired either by nonhomologous end-joining (NHEJ) or homologous end-joining. ...
Biotechnology Lab
Biotechnology Lab

... 5. Take glued pieces and put them into another bacterium (plasmid transformation of E. coli) 6. Separate bacteria with plasmid from those without ...
Ch08
Ch08

... b. two products of about the same size! One large band on electrophoresis that is about half the size of the original plasmid..and being two different nucleic acid products making the same band. c. three products: one from PvuII to EcoR1 site, the largest, the second from PvuII to BamH1, the next sm ...
DNA Studyguide - OG
DNA Studyguide - OG

... 26. What sugar is found on RNA, as compared to DNA? 27. What base is missing on RNA, & what other base replaces it? 28. Uracil will pair with what other base on DNA? ...
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Homologous recombination



Homologous recombination is a type of genetic recombination in which nucleotide sequences are exchanged between two similar or identical molecules of DNA. It is most widely used by cells to accurately repair harmful breaks that occur on both strands of DNA, known as double-strand breaks. Homologous recombination also produces new combinations of DNA sequences during meiosis, the process by which eukaryotes make gamete cells, like sperm and egg cells in animals. These new combinations of DNA represent genetic variation in offspring, which in turn enables populations to adapt during the course of evolution. Homologous recombination is also used in horizontal gene transfer to exchange genetic material between different strains and species of bacteria and viruses.Although homologous recombination varies widely among different organisms and cell types, most forms involve the same basic steps. After a double-strand break occurs, sections of DNA around the 5' ends of the break are cut away in a process called resection. In the strand invasion step that follows, an overhanging 3' end of the broken DNA molecule then ""invades"" a similar or identical DNA molecule that is not broken. After strand invasion, the further sequence of events may follow either of two main pathways discussed below (see Models); the DSBR (double-strand break repair) pathway or the SDSA (synthesis-dependent strand annealing) pathway. Homologous recombination that occurs during DNA repair tends to result in non-crossover products, in effect restoring the damaged DNA molecule as it existed before the double-strand break.Homologous recombination is conserved across all three domains of life as well as viruses, suggesting that it is a nearly universal biological mechanism. The discovery of genes for homologous recombination in protists—a diverse group of eukaryotic microorganisms—has been interpreted as evidence that meiosis emerged early in the evolution of eukaryotes. Since their dysfunction has been strongly associated with increased susceptibility to several types of cancer, the proteins that facilitate homologous recombination are topics of active research. Homologous recombination is also used in gene targeting, a technique for introducing genetic changes into target organisms. For their development of this technique, Mario Capecchi, Martin Evans and Oliver Smithies were awarded the 2007 Nobel Prize for Physiology or Medicine.
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