Learning objectives
... Read pages 336-359 of “Biology” Miller & Levine (Chapter 12) and the photocopied supplements from Biology Campbell & Reece (Chapter 16) to make your Cornell notes and understand the following learning objectives. Remember these are NOT questions but guidelines for your note taking. Reading for compr ...
... Read pages 336-359 of “Biology” Miller & Levine (Chapter 12) and the photocopied supplements from Biology Campbell & Reece (Chapter 16) to make your Cornell notes and understand the following learning objectives. Remember these are NOT questions but guidelines for your note taking. Reading for compr ...
Chapter 47
... electrophoresis, DNA polymerase, complementary binding northern: electrophoresis, RNA transfer, probe hybridization, complementary binding, autoradiography, expose RT-PCR: mRNA, reverse transcriptase, cDNA Microarrays: mRNA isolation, cDNA, probe hybridization, complementary binding) 7. Describe how ...
... electrophoresis, DNA polymerase, complementary binding northern: electrophoresis, RNA transfer, probe hybridization, complementary binding, autoradiography, expose RT-PCR: mRNA, reverse transcriptase, cDNA Microarrays: mRNA isolation, cDNA, probe hybridization, complementary binding) 7. Describe how ...
DNA PPT
... We study DNA for many reasons: its central importance to all life on Earth, medical benefits such as cures for diseases, better food crops. ...
... We study DNA for many reasons: its central importance to all life on Earth, medical benefits such as cures for diseases, better food crops. ...
Messenger RNA
... It stores all of our genetic information It’s function is to tell the cell what proteins to make and when to make them. Remember: it is the brain of the cell. ...
... It stores all of our genetic information It’s function is to tell the cell what proteins to make and when to make them. Remember: it is the brain of the cell. ...
Chapter 6
... 1950’s – Erwin Chargaff found the amount in adenine in DNA always matches the amount of thymine Also, the amount of cytosine always matches the amount of guanine Clue 1 to the structure of DNA ...
... 1950’s – Erwin Chargaff found the amount in adenine in DNA always matches the amount of thymine Also, the amount of cytosine always matches the amount of guanine Clue 1 to the structure of DNA ...
building linear and plasmid dna models
... 4. Using the sequence above, attach the first base (C – green tube) to the first sugar in the DNA strand. Attach each successive base to only the sugars. ...
... 4. Using the sequence above, attach the first base (C – green tube) to the first sugar in the DNA strand. Attach each successive base to only the sugars. ...
DNA Replication and DNA Repair Study Guide Focus on the
... i. Beginning point of replication ii. Prokaryotes (bacteria)- 1 origin of replication iii. Eukaryotes- 1 to 2000 origins of replication per chromosome b. Direction- two forks proceed in opposite directions c. Forks i. Replication sites ii. Proceed in one direction (one for each direction) iii. Repli ...
... i. Beginning point of replication ii. Prokaryotes (bacteria)- 1 origin of replication iii. Eukaryotes- 1 to 2000 origins of replication per chromosome b. Direction- two forks proceed in opposite directions c. Forks i. Replication sites ii. Proceed in one direction (one for each direction) iii. Repli ...
12-2 Notes
... Structure of DNA There are four nitrogenous bases that belong in two categories Purines – have two rings in their structure ...
... Structure of DNA There are four nitrogenous bases that belong in two categories Purines – have two rings in their structure ...
The Central Dogma of Biology DNA → RNA→ Protein
... - DNA was actually discovered in the 1860’s - DNA is made of only 4 nucleotides (Adenine, Thymine, Guanine, and Cytosine) also known as bases - initially DNA was considered interesting but too simple to be important! - a molecule of DNA has 2 strands held together by hydrogen bonds (hydrogen bonds a ...
... - DNA was actually discovered in the 1860’s - DNA is made of only 4 nucleotides (Adenine, Thymine, Guanine, and Cytosine) also known as bases - initially DNA was considered interesting but too simple to be important! - a molecule of DNA has 2 strands held together by hydrogen bonds (hydrogen bonds a ...
answers
... What is a purine? NITROGEN BASE WITH 2 RINGS What is a pyrimidine? NITROGEN BASE WITH ONE RING What is the shape of a DNA molecule? DOUBLE HELIX= “TWISTED LADDER” Which molecules for the backbone of the DNA molecule? PHOSPHATES__ & __SUGARS_______ What molecules form the “steps of the ladder”? ___NI ...
... What is a purine? NITROGEN BASE WITH 2 RINGS What is a pyrimidine? NITROGEN BASE WITH ONE RING What is the shape of a DNA molecule? DOUBLE HELIX= “TWISTED LADDER” Which molecules for the backbone of the DNA molecule? PHOSPHATES__ & __SUGARS_______ What molecules form the “steps of the ladder”? ___NI ...
DNAfor NathanLec - Sonoma State University
... a. Form a bond between - 3’ OH group of deoxyribose on last nucleotide - 5’ phosphate of the correct dNTP b. Add nucleotide and release diphosphate c. Move to next nucleotide on template B. Initiation of DNA replication 1. Origins of replication a. Specific sequence of nucleotides b. recognized by p ...
... a. Form a bond between - 3’ OH group of deoxyribose on last nucleotide - 5’ phosphate of the correct dNTP b. Add nucleotide and release diphosphate c. Move to next nucleotide on template B. Initiation of DNA replication 1. Origins of replication a. Specific sequence of nucleotides b. recognized by p ...
Concept 11-1 & 11-2
... - When only phage protein coats were labeled, most of the radioactivity was detected outside the cells. But when phage DNA was labeled, most of the radioactivity was detected inside the cells. - Hershey and Chase offered further evidence that DNA, not proteins, is the genetic material. Only the DNA ...
... - When only phage protein coats were labeled, most of the radioactivity was detected outside the cells. But when phage DNA was labeled, most of the radioactivity was detected inside the cells. - Hershey and Chase offered further evidence that DNA, not proteins, is the genetic material. Only the DNA ...
EXAM 1
... MET STOP or no protein/polypeptide produced 20. The following has TWO parts. The mRNA sequence above is from the 5’ end of the CAP gene, a regulator of the lac operon. In the face of the insertion mutation described above, would the gene be transcribed at relatively high or low levels if the bacteri ...
... MET STOP or no protein/polypeptide produced 20. The following has TWO parts. The mRNA sequence above is from the 5’ end of the CAP gene, a regulator of the lac operon. In the face of the insertion mutation described above, would the gene be transcribed at relatively high or low levels if the bacteri ...
Taq Polymerase - cloudfront.net
... Taq polymerase can withstand temperatures needed to produce the best effects in this machine, so scientists can run many PCR cycles automatically. PCR involves denaturing, annealing and replication steps, usually repeated 20 to 30 times. Denaturing separates the double-stranded DNA into single stran ...
... Taq polymerase can withstand temperatures needed to produce the best effects in this machine, so scientists can run many PCR cycles automatically. PCR involves denaturing, annealing and replication steps, usually repeated 20 to 30 times. Denaturing separates the double-stranded DNA into single stran ...
Table 3.
... Design primers for shorter amplicon length and flank melt domains. Low PCR yield Optimize PCR to enhance product yield. Optimize PCR conditions to obtain clean product or design new primers without secondary structures. ...
... Design primers for shorter amplicon length and flank melt domains. Low PCR yield Optimize PCR to enhance product yield. Optimize PCR conditions to obtain clean product or design new primers without secondary structures. ...
Lab22
... 1. Double stranded DNA template must be separated 2. DNA primers base pair to ends of single stranded target sequence 3. DNA polymerase adds nucleotides to the 3’ end of the primers by complementary base pairing free nucleotides to the template strand 4. Repeat steps 1-3: each new copy generated can ...
... 1. Double stranded DNA template must be separated 2. DNA primers base pair to ends of single stranded target sequence 3. DNA polymerase adds nucleotides to the 3’ end of the primers by complementary base pairing free nucleotides to the template strand 4. Repeat steps 1-3: each new copy generated can ...
Worksheet for Biology 1107 Biological Molecules: Structure and
... 9. List the amino acids that are in the primary structure of the peptide on page 5 of the biomolecules text. ...
... 9. List the amino acids that are in the primary structure of the peptide on page 5 of the biomolecules text. ...
Slide 1
... code for making proteins) goes to a ribosome. • At the ribosome pieces of tRNA (transfer RNA) bring amino acids to the ribosome and match them up with the code on the codon to make a protein. • Proteins are just a string of amino acids. ...
... code for making proteins) goes to a ribosome. • At the ribosome pieces of tRNA (transfer RNA) bring amino acids to the ribosome and match them up with the code on the codon to make a protein. • Proteins are just a string of amino acids. ...
Ch. 11
... the form of a ____________________________ B. Replication of DNA a. ____________________________ ______ – the copying of DNA chromosomes. Occurs in interphase 1. DNA Synthesis (replication) a. _______________(DNA Polymerase) unzip the DNA strand b. Free ____________________________ bond w/ open base ...
... the form of a ____________________________ B. Replication of DNA a. ____________________________ ______ – the copying of DNA chromosomes. Occurs in interphase 1. DNA Synthesis (replication) a. _______________(DNA Polymerase) unzip the DNA strand b. Free ____________________________ bond w/ open base ...
DNA polymerase
The DNA polymerases are enzymes that create DNA molecules by assembling nucleotides, the building blocks of DNA. These enzymes are essential to DNA replication and usually work in pairs to create two identical DNA strands from a single original DNA molecule. During this process, DNA polymerase “reads” the existing DNA strands to create two new strands that match the existing ones.Every time a cell divides, DNA polymerase is required to help duplicate the cell’s DNA, so that a copy of the original DNA molecule can be passed to each of the daughter cells. In this way, genetic information is transmitted from generation to generation.Before replication can take place, an enzyme called helicase unwinds the DNA molecule from its tightly woven form. This opens up or “unzips” the double-stranded DNA to give two single strands of DNA that can be used as templates for replication.