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DNA review
DNA review

... ___________________________ Process of making a complementary RNA message from a DNA code (DNA  RNA) ___________________________ Process of making copy of a DNA molecule (DNA  DNA) ___________________________ Process of making a protein from an RNA message (RNAPROTEIN) ___________________________ ...
Expanding the DNA alphabet: `Extra` DNA base found to
Expanding the DNA alphabet: `Extra` DNA base found to

... brain tissue pointed to the fact that 5fC can be a stable modification: if it was a transient molecule, this uptake of isotopes would be high. The researchers believe that 5fC might alter the way DNA is recognised by proteins. "Unmodified DNA interacts with a specific set of proteins, and the presen ...
From Gene to Protein Part 2
From Gene to Protein Part 2

... FROM GENE TO PROTEIN PART 2 Goal 1- Understand the process of transcription • How is RNA made? •How ...
Document
Document

Genetics Introduction:
Genetics Introduction:

...  Pyrimidines- C and T (single ring) o Pairing between nucleotides of double helix is complementary- adenine pairs with thymine, guanine pairs with cytosine o Each DNA strand has polarity= directionality o Paired strands are oriented in opposite directions= antiparallel ...
Molecular genetics of bacteria
Molecular genetics of bacteria

... DNA replication • Origin of DNA replication: particular site on DNA where copying of the DNA always starts. – Replication is bidirectional – In each direction, there is a replication fork. – Bacterial DNA is circular, so there is one Origin and one ...
MICROBIAL GENETICS
MICROBIAL GENETICS

... Sugar back bones are linked to each other by phosphodiester bonds, i.e., a single phosphate connected by ester linkage to two sugars; DNA consists of alternating units of phosphate and 2 deoxy ribose ...
No Slide Title
No Slide Title

... of growing chain (condensation rx driven by cleavage of PiPi) ...
CH-13 Sect 1
CH-13 Sect 1

... which is a small circular DNA molecule found naturally in some bacteria. The culture is treated with a(an) ______________, a compound that kills bacteria ...
"DNA The Primary Frontier"
"DNA The Primary Frontier"

... of DNA. Here, within these DNA nucleotides, lie answers to the mysteries of life’s genetic diseases. The mission of the Next Generation DNA Sequencing Core Facility (NGS) is to sequence large amounts of DNA quickly and inexpensively, said Director Jim Eudy, Ph.D. “It took $2.7 billion and a factory ...
BioDynami 1 kb plus DNA ladder, ready-to-load
BioDynami 1 kb plus DNA ladder, ready-to-load

... Storage: at 4°C for periods up to 6 months. For longer periods, store at -20°C. Description  For sizing and quantification of double strand DNA fragments.  Composed of 13 bands as shown on right. The 10 kb and 4 kb bands with higher concentration are easily distinguishable from the others.  Premi ...
Epigenetics Presentation_BiologicalAffinity
Epigenetics Presentation_BiologicalAffinity

... analysis; Cons: DNA must be single-stranded, must have quality enzymes or risk false-data, method has inherent bias for specific sequences  MAP – Pros: Outdated and thus cheap, works easily and quickly with large scale, relatively easy (computationally); Cons – outdated, needs a large amount of DNA ...
Module 16 - MDC Faculty Web Pages
Module 16 - MDC Faculty Web Pages

... 3 nitrogen-containing base found in DNA (derived from pyrimidine) 6 nitrogen-containing base found in RNA (derived from pyrimidine) 9 the five-carbon pentose sugar found in DNA 11 _______ RNA is produced in the nucleus by DNA to carry the genetic information to the ribosomes 13 the double _______ is ...
Central Dogma DNA RNA Protein Lecture 10
Central Dogma DNA RNA Protein Lecture 10

... polymerase adds nuclotides at 3’ end of existing strand Synthesis occurs in 5’ - 3’ direction ...
DNA TAKS QUESTIONS SPRING 2003 – 11: (38) In DNA, which of
DNA TAKS QUESTIONS SPRING 2003 – 11: (38) In DNA, which of

... 45 Which molecule is most responsible for determining an organism’s eye color, body structure, and cellular enzyme production? A Complex starch B Fatty acid C Carbohydrate D* Deoxyribonucleic acid OCTOBER 2005 – 11: 35 Proteins are produced according to a special code found in the control center of ...
Molecular Genetics 2- Central Dogma PDQ
Molecular Genetics 2- Central Dogma PDQ

... 1. Diagram the “Central Dogma” of molecular genetics. How does it allow for DNA to serve as both the heritable molecule and code for protein sequence? 2. Explain the experiment conducted by Meselson and Stahl. How did the results of their experiment demonstrate the semi-conservative model of DNA rep ...
Common types of DNA damage Different types of repair fix different
Common types of DNA damage Different types of repair fix different

... at which point these subunits form a highly error-prone polymerase pol V (UmuD’2UmuC), which then finds a stalled DNA pol III, adds dNTPs without really looking at the template, and after adding a few dNTP gets displaced by pol III. ...
RNA removal by double-RNase digestion
RNA removal by double-RNase digestion

... of the amount of DNA. This protocol describes the use of double-RNase digestion to remove the RNA in Oragene/saliva samples. After this RNase treatment, the DNA samples will give similar quantification results by absorbance or fluorescence. ...
DNA and RNA
DNA and RNA

...  When it makes a copy, one half of the old strand is ...
Targeted Fluorescent Reporters: Additional slides
Targeted Fluorescent Reporters: Additional slides

... 10. In the first step of proofreading by DNA polymerase, the moving DNA polymerase has a higher affinity for the correct nucleotide than an incorrect one because only the correct one can base pair with the template. 11. After nucleotide binding, but before the nucleotide is covalently bonded to the ...
DNA and RNA ppt
DNA and RNA ppt

...  When it makes a copy, one half of the old strand is ...
Whole Genome Scale DNA Methylation Differences in
Whole Genome Scale DNA Methylation Differences in

... We have established a protocol for thymocyte and stromal cell isolation and good quality DNA and RNA from these paired samples from the same individual. In addition, fresh thymic tissue was mounted in preservative blocks and frozen for later use in microscopy studies and for nPOD collection. Summary ...
specific location on chromosome, consisting of a segment of DNA
specific location on chromosome, consisting of a segment of DNA

... 3. PROTEIN: large, complex polymer essential to all life. Composed of carbon, hydrogen, oxygen, nitrogen, and usually sulfur; provides structure for tissues and organs and helps carry out cell metabolism 4. CHROMOSOME: structure in the nucleus of a cell consisting of long thread of DNA that is ...
File
File

DNA & DNA Replication
DNA & DNA Replication

... Other daughter strand is also synthesized 5’3’ because that is only way that DNA can be assembled  However the template is also being read ...
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DNA polymerase



The DNA polymerases are enzymes that create DNA molecules by assembling nucleotides, the building blocks of DNA. These enzymes are essential to DNA replication and usually work in pairs to create two identical DNA strands from a single original DNA molecule. During this process, DNA polymerase “reads” the existing DNA strands to create two new strands that match the existing ones.Every time a cell divides, DNA polymerase is required to help duplicate the cell’s DNA, so that a copy of the original DNA molecule can be passed to each of the daughter cells. In this way, genetic information is transmitted from generation to generation.Before replication can take place, an enzyme called helicase unwinds the DNA molecule from its tightly woven form. This opens up or “unzips” the double-stranded DNA to give two single strands of DNA that can be used as templates for replication.
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