Amplification and partial sequencing of Ixodes Scapularis Shaker
... undoubtedly expected to be present in the tick, the project easily provided a partial sequence of the voltage gated potassium channel. Although the elongase kit had been used, it had only been used twice within the entire experiment so the conditions had not been optimized for amplifying the tick an ...
... undoubtedly expected to be present in the tick, the project easily provided a partial sequence of the voltage gated potassium channel. Although the elongase kit had been used, it had only been used twice within the entire experiment so the conditions had not been optimized for amplifying the tick an ...
Protocol for RiboShredder™ RNase Blend
... Unit Definition: One unit completely degrades the total RNA component in a reaction consisting of 1 μl of RiboShredder RNase Blend and 10 μl of substrate (13 μg/μl of nucleic acid in T10E1 buffer) in 10 minutes at 37°C. The assay substrate is the total nucleic acid recovered from a standard alkaline ...
... Unit Definition: One unit completely degrades the total RNA component in a reaction consisting of 1 μl of RiboShredder RNase Blend and 10 μl of substrate (13 μg/μl of nucleic acid in T10E1 buffer) in 10 minutes at 37°C. The assay substrate is the total nucleic acid recovered from a standard alkaline ...
Restriction Digest of pAMP and pKAN
... the agarose matrix, they will migrate faster than the larger fragments. Picture a group of crosscountry runners that are racing through a dense tropical rain forest. All other factors being equal, the shorter runners will be able to navigate through the tangle of overhanging vines and dense foliage ...
... the agarose matrix, they will migrate faster than the larger fragments. Picture a group of crosscountry runners that are racing through a dense tropical rain forest. All other factors being equal, the shorter runners will be able to navigate through the tangle of overhanging vines and dense foliage ...
American College of Medical Genetics and Genomics
... to alert laboratories to the need to establish a reporting policy in conjunction with their ethics review committee and legal counsel. ...
... to alert laboratories to the need to establish a reporting policy in conjunction with their ethics review committee and legal counsel. ...
Novel DNA Polymerase Increases Efficiency of Multiple PCR
... long-range PCR, LAMP, and PCDR, a recently developed method that was presented in another Biotechniques paper published in February 2013. First, the researchers compared the properties of SD polymerase to Bst polymerase in LAMP, carrying out reactions at 63° C with or without an initial two-minute, ...
... long-range PCR, LAMP, and PCDR, a recently developed method that was presented in another Biotechniques paper published in February 2013. First, the researchers compared the properties of SD polymerase to Bst polymerase in LAMP, carrying out reactions at 63° C with or without an initial two-minute, ...
Table of Contents
... 2. What are the advantages to using Phusion® High-Fidelity DNA Polymerase? Phusion® High-Fidelity DNA Polymerase's processivity-enhancing domain results in shorter extension times, more robust and high yield amplification, and the ability to extend long templates in a fraction of the time, making Ph ...
... 2. What are the advantages to using Phusion® High-Fidelity DNA Polymerase? Phusion® High-Fidelity DNA Polymerase's processivity-enhancing domain results in shorter extension times, more robust and high yield amplification, and the ability to extend long templates in a fraction of the time, making Ph ...
ISOLATE II PCR and Gel Kit
... Molecular biology grade ethanol is recommended. Do not use denatured alcohol which contains unwanted additives such as methanol and acetone. ...
... Molecular biology grade ethanol is recommended. Do not use denatured alcohol which contains unwanted additives such as methanol and acetone. ...
Distortion of quantitative genomic and expression
... regarding reproducibility of these techniques have been raised by cross-validation studies in different laboratories (1–5). Strategies to mitigate variability in the results obtained from replicate studies have focused on standardizing technical factors, such as array production, RNA synthesis, labe ...
... regarding reproducibility of these techniques have been raised by cross-validation studies in different laboratories (1–5). Strategies to mitigate variability in the results obtained from replicate studies have focused on standardizing technical factors, such as array production, RNA synthesis, labe ...
video slide
... It also contains the lacZ gene, which encodes -galactosidase. This enzyme hydrolyzes a molecular mimic of lactose (X-gal) to form a blue product. Only three plasmids and three human DNA fragments are shown, but millions of copies of the plasmid and a mixture of millions of different human DNA fragm ...
... It also contains the lacZ gene, which encodes -galactosidase. This enzyme hydrolyzes a molecular mimic of lactose (X-gal) to form a blue product. Only three plasmids and three human DNA fragments are shown, but millions of copies of the plasmid and a mixture of millions of different human DNA fragm ...
Crystal structure of the nucleosome core particle at 2.8 Å
... concentration, and on histone-modification state (4). For example, the variability of nucleosome spacing within in vitro-assembled arrays demonstrates the underlying electrostatic component of the interaction of DNA with the basic histone proteins (5). The characteristics of an individual nucleosome ...
... concentration, and on histone-modification state (4). For example, the variability of nucleosome spacing within in vitro-assembled arrays demonstrates the underlying electrostatic component of the interaction of DNA with the basic histone proteins (5). The characteristics of an individual nucleosome ...
PowerPoint-presentatie - the biopsychology research group
... exon resequencing data, and Perlegen data were included. For Caucasian samples, the average r2 between a SNP in the HapMap and a working assay is 0.94. The percentage of SNPs in the HapMap with r2 > 0.8 with a Perlegen SNP is 93%. ...
... exon resequencing data, and Perlegen data were included. For Caucasian samples, the average r2 between a SNP in the HapMap and a working assay is 0.94. The percentage of SNPs in the HapMap with r2 > 0.8 with a Perlegen SNP is 93%. ...
Cytogenetics with special reference to domestic animals
... Sheep: (Differences between species often involve Roberstonian fusions) ...
... Sheep: (Differences between species often involve Roberstonian fusions) ...
Analysis and Characterization of Nucleic Acids and Proteins
... procedure allows analysis of any specific gene or region without having to separate it from a complex background. ...
... procedure allows analysis of any specific gene or region without having to separate it from a complex background. ...
Comparative genomic hybridization
Comparative genomic hybridization is a molecular cytogenetic method for analysing copy number variations (CNVs) relative to ploidy level in the DNA of a test sample compared to a reference sample, without the need for culturing cells. The aim of this technique is to quickly and efficiently compare two genomic DNA samples arising from two sources, which are most often closely related, because it is suspected that they contain differences in terms of either gains or losses of either whole chromosomes or subchromosomal regions (a portion of a whole chromosome). This technique was originally developed for the evaluation of the differences between the chromosomal complements of solid tumor and normal tissue, and has an improved resoIution of 5-10 megabases compared to the more traditional cytogenetic analysis techniques of giemsa banding and fluorescence in situ hybridization (FISH) which are limited by the resolution of the microscope utilized.This is achieved through the use of competitive fluorescence in situ hybridization. In short, this involves the isolation of DNA from the two sources to be compared, most commonly a test and reference source, independent labelling of each DNA sample with a different fluorophores (fluorescent molecules) of different colours (usually red and green), denaturation of the DNA so that it is single stranded, and the hybridization of the two resultant samples in a 1:1 ratio to a normal metaphase spread of chromosomes, to which the labelled DNA samples will bind at their locus of origin. Using a fluorescence microscope and computer software, the differentially coloured fluorescent signals are then compared along the length of each chromosome for identification of chromosomal differences between the two sources. A higher intensity of the test sample colour in a specific region of a chromosome indicates the gain of material of that region in the corresponding source sample, while a higher intensity of the reference sample colour indicates the loss of material in the test sample in that specific region. A neutral colour (yellow when the fluorophore labels are red and green) indicates no difference between the two samples in that location.CGH is only able to detect unbalanced chromosomal abnormalities. This is because balanced chromosomal abnormalities such as reciprocal translocations, inversions or ring chromosomes do not affect copy number, which is what is detected by CGH technologies. CGH does, however, allow for the exploration of all 46 human chromosomes in single test and the discovery of deletions and duplications, even on the microscopic scale which may lead to the identification of candidate genes to be further explored by other cytological techniques.Through the use of DNA microarrays in conjunction with CGH techniques, the more specific form of array CGH (aCGH) has been developed, allowing for a locus-by-locus measure of CNV with increased resolution as low as 100 kilobases. This improved technique allows for the aetiology of known and unknown conditions to be discovered.