Influence of the environment and probes on rapid DNA sequencing
... While the sampling frequency can be determined exactly, it is hard to give an exact value of the time needed in between two instantaneous measurements for them to be considered independent. However, we can take the timescale for atomic movements in the simulation, which is about 1 ps, as a rough est ...
... While the sampling frequency can be determined exactly, it is hard to give an exact value of the time needed in between two instantaneous measurements for them to be considered independent. However, we can take the timescale for atomic movements in the simulation, which is about 1 ps, as a rough est ...
PDF version - EpiGeneSys
... Nucleosome array assembly Basic principles of the reconstitution protocol: 1. DNA arrays are constructed using the Widom 601 nucleosome positioning DNA sequence. DNA arrays containing between 12 and 80 tandem 601 DNA repeats with different nucleosome repeat lengths (NRLs) (167 to 237bp) have been co ...
... Nucleosome array assembly Basic principles of the reconstitution protocol: 1. DNA arrays are constructed using the Widom 601 nucleosome positioning DNA sequence. DNA arrays containing between 12 and 80 tandem 601 DNA repeats with different nucleosome repeat lengths (NRLs) (167 to 237bp) have been co ...
Analysis of Similarities/Dissimilarities of DNA Sequences Based on a
... -496proposed [3–15]. Nandy [5] constructed a graphical model by assigning A, G, T and C to the four directions, (−x), (+x), (−y) and (+y), respectively, along the positive and the negative Cartesian coordinate axes. But such a representation of DNA is accompanied by some loss of information associa ...
... -496proposed [3–15]. Nandy [5] constructed a graphical model by assigning A, G, T and C to the four directions, (−x), (+x), (−y) and (+y), respectively, along the positive and the negative Cartesian coordinate axes. But such a representation of DNA is accompanied by some loss of information associa ...
Novel cryptic chromosomal rearrangements in childhood acute
... IGH spanning probe (green), and fusion of one signal with the LSI® D20S108 (red) located at 20q12 (arrowhead). (A4) Partial G-banding (B1) and M-FISH analysis in patient #2 revealed a recurrent t(1;14)(p32;q11), and 2 cryptic translocations (B2). Dual color FISH with chromosome 11 library (red) and ...
... IGH spanning probe (green), and fusion of one signal with the LSI® D20S108 (red) located at 20q12 (arrowhead). (A4) Partial G-banding (B1) and M-FISH analysis in patient #2 revealed a recurrent t(1;14)(p32;q11), and 2 cryptic translocations (B2). Dual color FISH with chromosome 11 library (red) and ...
Recognition of an organism from fragments of its complete genome
... distinguish bacteria because the computed fractal dimensions of bacteria 关22兴 are the same. The classification and evolution relationship of bacteria is one of the most important problems in DNA research. Yu and Anh 关23兴 proposed a time series model based on the global structure of the complete geno ...
... distinguish bacteria because the computed fractal dimensions of bacteria 关22兴 are the same. The classification and evolution relationship of bacteria is one of the most important problems in DNA research. Yu and Anh 关23兴 proposed a time series model based on the global structure of the complete geno ...
Balanced reciprocal translocation at amniocentesis - FUNPEC-RP
... found that 14 children were born with a normal phenotype. Pregnancy was terminated in one case, and follow-up was refused in another (Table 1). ...
... found that 14 children were born with a normal phenotype. Pregnancy was terminated in one case, and follow-up was refused in another (Table 1). ...
Ch12_lecture - Dr. Brahmbhatt`s Class Handouts
... Forensic Science? Gel electrophoresis separates DNA segments. • A mixture of DNA pieces is separated by a technique called gel electrophoresis. • The mixture of DNA is loaded onto a slab of agarose. • The gel is put in a chamber with electrodes connected to each end; one is positive, the other neg ...
... Forensic Science? Gel electrophoresis separates DNA segments. • A mixture of DNA pieces is separated by a technique called gel electrophoresis. • The mixture of DNA is loaded onto a slab of agarose. • The gel is put in a chamber with electrodes connected to each end; one is positive, the other neg ...
Huntingtin grabs a hammer: DNA repair in HD
... Mutant huntingtin comes to work and botches the job In HD mice, lowering ATM levels was beneficial, perhaps because it dulled ATM’s misdirected DNA repairs and zealous demolitions. But if huntingtin itself helps fix DNA lesions, perhaps there’s a difference in how normal versus mutant huntingtin mov ...
... Mutant huntingtin comes to work and botches the job In HD mice, lowering ATM levels was beneficial, perhaps because it dulled ATM’s misdirected DNA repairs and zealous demolitions. But if huntingtin itself helps fix DNA lesions, perhaps there’s a difference in how normal versus mutant huntingtin mov ...
Chapter 4. Studying DNA Learning outcomes 4.1. Enzymes for DNA
... agarose gel electrophoresis (see Technical Note 2.1) to determine their sizes. Depending on the concentration of agarose in the gel, fragments between 100 bp and 50 kb can be separated into sharp bands after electrophoresis ( Figure 4.11 ). Fragments less than 150 bp can be separated in a 4% or 5% a ...
... agarose gel electrophoresis (see Technical Note 2.1) to determine their sizes. Depending on the concentration of agarose in the gel, fragments between 100 bp and 50 kb can be separated into sharp bands after electrophoresis ( Figure 4.11 ). Fragments less than 150 bp can be separated in a 4% or 5% a ...
Processivity of DNA polymerases: two mechanisms, one goal
... regulators (e.g. p21/Cip1 and Gadd45) have been shown to interact with the PCNA clamp and they affect its function during the cell cycle and DNA repair [8]. PCNA also interacts with the enzymes needed for Okazaki fragment maturation (e.g. Fen-1 and DNA ligase I) [19] and postreplication events (e.g. ...
... regulators (e.g. p21/Cip1 and Gadd45) have been shown to interact with the PCNA clamp and they affect its function during the cell cycle and DNA repair [8]. PCNA also interacts with the enzymes needed for Okazaki fragment maturation (e.g. Fen-1 and DNA ligase I) [19] and postreplication events (e.g. ...
FX 8 - ACMG
... insufficiency (FXPOI). Fragile X DNA studies are recommended for other at-risk family members. Prenatal diagnosis is available for all future pregnancies, since expansion to a full mutation can occur. Genetic counseling is recommended. Due to the nature of the assay, we estimate that the number of C ...
... insufficiency (FXPOI). Fragile X DNA studies are recommended for other at-risk family members. Prenatal diagnosis is available for all future pregnancies, since expansion to a full mutation can occur. Genetic counseling is recommended. Due to the nature of the assay, we estimate that the number of C ...
Chapter 13 Chromosomes
... retardation, and increased risk of a heart defect, suppressed immunity, and leukemia. ...
... retardation, and increased risk of a heart defect, suppressed immunity, and leukemia. ...
The rapidly evolving field of plant centromeres
... arenosa and Olimarabidopsis pumila did not reveal conserved regions within these closely related genera [19]. By contrast, CentO and CentC maintain similar 50 and 30 ends despite a divergence of 50–70 million years [8,20]. In maize, analysis of satellite polymorphisms made it possible to define at ...
... arenosa and Olimarabidopsis pumila did not reveal conserved regions within these closely related genera [19]. By contrast, CentO and CentC maintain similar 50 and 30 ends despite a divergence of 50–70 million years [8,20]. In maize, analysis of satellite polymorphisms made it possible to define at ...
REAL-TIME PCR
... Can SYBR green assays be used for tests that require high specificity? No, because SYBR green molecules bind non-specifically to any dsDNA, they will also bind to nonspecific products if present in a reaction. ...
... Can SYBR green assays be used for tests that require high specificity? No, because SYBR green molecules bind non-specifically to any dsDNA, they will also bind to nonspecific products if present in a reaction. ...
Li 2001 Cancer Res
... Houston metropolitan area (Kelsey Seybold Clinic) during the same time period (8). These control subjects were first surveyed with a short questionnaire for willingness to participate in research studies and to provide data on smoking behavior and demographics. A computer database of more than 50,00 ...
... Houston metropolitan area (Kelsey Seybold Clinic) during the same time period (8). These control subjects were first surveyed with a short questionnaire for willingness to participate in research studies and to provide data on smoking behavior and demographics. A computer database of more than 50,00 ...
- Peanut Science
... Several methods are used to distinguish hybrid versus selfed progenies, including observing morphological differences among progenies, segregation for disease resistance or differences in the oleic:linoleic ratio (López and Burow, unpublished results). Identifying hybrids in the F1 generation can b ...
... Several methods are used to distinguish hybrid versus selfed progenies, including observing morphological differences among progenies, segregation for disease resistance or differences in the oleic:linoleic ratio (López and Burow, unpublished results). Identifying hybrids in the F1 generation can b ...
Structural analysis of both products of a reciprocal translocation
... Comparative r e s t r i c t i o n mapping and b l o t hybridization experiments showed that this fragment contains Immunoglobulin heavy chain sequences extending from J5 to the 5' ...
... Comparative r e s t r i c t i o n mapping and b l o t hybridization experiments showed that this fragment contains Immunoglobulin heavy chain sequences extending from J5 to the 5' ...
Comparative genomic hybridization
Comparative genomic hybridization is a molecular cytogenetic method for analysing copy number variations (CNVs) relative to ploidy level in the DNA of a test sample compared to a reference sample, without the need for culturing cells. The aim of this technique is to quickly and efficiently compare two genomic DNA samples arising from two sources, which are most often closely related, because it is suspected that they contain differences in terms of either gains or losses of either whole chromosomes or subchromosomal regions (a portion of a whole chromosome). This technique was originally developed for the evaluation of the differences between the chromosomal complements of solid tumor and normal tissue, and has an improved resoIution of 5-10 megabases compared to the more traditional cytogenetic analysis techniques of giemsa banding and fluorescence in situ hybridization (FISH) which are limited by the resolution of the microscope utilized.This is achieved through the use of competitive fluorescence in situ hybridization. In short, this involves the isolation of DNA from the two sources to be compared, most commonly a test and reference source, independent labelling of each DNA sample with a different fluorophores (fluorescent molecules) of different colours (usually red and green), denaturation of the DNA so that it is single stranded, and the hybridization of the two resultant samples in a 1:1 ratio to a normal metaphase spread of chromosomes, to which the labelled DNA samples will bind at their locus of origin. Using a fluorescence microscope and computer software, the differentially coloured fluorescent signals are then compared along the length of each chromosome for identification of chromosomal differences between the two sources. A higher intensity of the test sample colour in a specific region of a chromosome indicates the gain of material of that region in the corresponding source sample, while a higher intensity of the reference sample colour indicates the loss of material in the test sample in that specific region. A neutral colour (yellow when the fluorophore labels are red and green) indicates no difference between the two samples in that location.CGH is only able to detect unbalanced chromosomal abnormalities. This is because balanced chromosomal abnormalities such as reciprocal translocations, inversions or ring chromosomes do not affect copy number, which is what is detected by CGH technologies. CGH does, however, allow for the exploration of all 46 human chromosomes in single test and the discovery of deletions and duplications, even on the microscopic scale which may lead to the identification of candidate genes to be further explored by other cytological techniques.Through the use of DNA microarrays in conjunction with CGH techniques, the more specific form of array CGH (aCGH) has been developed, allowing for a locus-by-locus measure of CNV with increased resolution as low as 100 kilobases. This improved technique allows for the aetiology of known and unknown conditions to be discovered.