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... activation. The known targets of this cascade in the wing disc are surprisingly limited, including repression of BRK and the novel extracellular protein encoded by pentagone (pent) (Vuilleumier et al., 2010), and activation of the transcription factors Optomotor Blind (OMB) (Grimm and Pflugfelder, 1 ...
... activation. The known targets of this cascade in the wing disc are surprisingly limited, including repression of BRK and the novel extracellular protein encoded by pentagone (pent) (Vuilleumier et al., 2010), and activation of the transcription factors Optomotor Blind (OMB) (Grimm and Pflugfelder, 1 ...
The green fluorescent protein: discovery
... During the 20th century the foundations of biochemistry were laid and used to explore the basal principles of the anabolic and catabolic pathways inside living cells. The 20th century also witnessed a revolution in our understanding of enzyme function and, through crystallography and nuclear magneti ...
... During the 20th century the foundations of biochemistry were laid and used to explore the basal principles of the anabolic and catabolic pathways inside living cells. The 20th century also witnessed a revolution in our understanding of enzyme function and, through crystallography and nuclear magneti ...
thèse - Université Evry Val d`Essonne
... of proto-mitochondrion formation with respect to the formation of the nucleus: either the endosimbiosis occurred at the same time as the formation of the eukaryotic cell, or it arrived only after the ancestral cell was already essentially eukaryotic. 1.1. The archaezoan scenario The archaezoan scen ...
... of proto-mitochondrion formation with respect to the formation of the nucleus: either the endosimbiosis occurred at the same time as the formation of the eukaryotic cell, or it arrived only after the ancestral cell was already essentially eukaryotic. 1.1. The archaezoan scenario The archaezoan scen ...
Purification of Arrestin from Bovine Retinas
... freshly bleached phosphorylated rhodopsin occurs rapidly (<200 msec) and quenches the activation of G protein (transducin) (1-4). We have shown that arrestin also acts in the phototransduction process by blocking rapid dephosphorylation of phosphorylated and photolyzed rhodopsin until activated rhod ...
... freshly bleached phosphorylated rhodopsin occurs rapidly (<200 msec) and quenches the activation of G protein (transducin) (1-4). We have shown that arrestin also acts in the phototransduction process by blocking rapid dephosphorylation of phosphorylated and photolyzed rhodopsin until activated rhod ...
Divergent Functional Properties of the Ribosome
... engineered just upstream of the ATG by PCR. This site was then used to clone a 3.2-kb fragment containing SSB into the BamHI site of pRSETB (Invitrogen, Carlsbad, CA), which contains six histidines and an anti-express antibody (Invitrogen) epitope. The gene encoding His-Ssb1 was moved as a 3.4-kb Xb ...
... engineered just upstream of the ATG by PCR. This site was then used to clone a 3.2-kb fragment containing SSB into the BamHI site of pRSETB (Invitrogen, Carlsbad, CA), which contains six histidines and an anti-express antibody (Invitrogen) epitope. The gene encoding His-Ssb1 was moved as a 3.4-kb Xb ...
Identification of a Cytoplasm to Vacuole Targeting Determinant in
... secretory pathway has been carefully documented for a number of vacuolar hydrolases (reviewed in Klionsky et al., 1990). These proteins translocate across the ER membrane via an NH2-terminal cleavable signal sequence or noncleaved internal hydrophobic domain. The signal sequence serves no further pu ...
... secretory pathway has been carefully documented for a number of vacuolar hydrolases (reviewed in Klionsky et al., 1990). These proteins translocate across the ER membrane via an NH2-terminal cleavable signal sequence or noncleaved internal hydrophobic domain. The signal sequence serves no further pu ...
Post-transcriptional regulation of auxin transport proteins: cellular
... Post-transcriptional regulation of auxin transport proteins | 1095 mutational analysis indicates that PIN3 functions in tropic responses, activation of its transport activity near sites of illumination could be expected to accelerate auxin movement out of tissues on the illuminated (non-bending sid ...
... Post-transcriptional regulation of auxin transport proteins | 1095 mutational analysis indicates that PIN3 functions in tropic responses, activation of its transport activity near sites of illumination could be expected to accelerate auxin movement out of tissues on the illuminated (non-bending sid ...
Scientific Opinion on Dietary Reference Values for protein
... European population on energy and macronutrients, including protein. ...
... European population on energy and macronutrients, including protein. ...
Tetrazine−trans-cyclooctene Mediated Conjugation of Antibodies to
... of the bonds under tension19 as well as at high gliding velocities,20 and reduced velocity of transport.21 The noncovalent biotin−streptavidin interaction is short-ranged and reversible, limiting its practical applications: examples are an almost million fold increase in the dissociation constant (K ...
... of the bonds under tension19 as well as at high gliding velocities,20 and reduced velocity of transport.21 The noncovalent biotin−streptavidin interaction is short-ranged and reversible, limiting its practical applications: examples are an almost million fold increase in the dissociation constant (K ...
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... epithelium. It is also highlighted by the arrest of tooth development at the bud stage in mice carrying either epithelial or mesenchymal inactivation of Catnb (Ctnnb1 – Mouse Genome Informatics), the gene encoding β-catenin, the master transducer of the canonical Wnt signaling (Sarkar and Sharpe, 19 ...
... epithelium. It is also highlighted by the arrest of tooth development at the bud stage in mice carrying either epithelial or mesenchymal inactivation of Catnb (Ctnnb1 – Mouse Genome Informatics), the gene encoding β-catenin, the master transducer of the canonical Wnt signaling (Sarkar and Sharpe, 19 ...
Sequence and Structure Classification of Kinases
... such as SCOP,3 CATH,4 and Pfam5 have been developed for the purpose of cataloging all protein sequences and structures. Here, we present the classification of a single group of proteins that catalyze a phosphoryl transfer reaction, and we subsequently examine the relationships between the fold and b ...
... such as SCOP,3 CATH,4 and Pfam5 have been developed for the purpose of cataloging all protein sequences and structures. Here, we present the classification of a single group of proteins that catalyze a phosphoryl transfer reaction, and we subsequently examine the relationships between the fold and b ...
as a PDF - CiteSeerX
... pepsin. The intermediate form was relatively stable as compared with pepsinogen, but was gradually converted to pepsin during a long period of incubation. When analyzed by the proteolytic activity assay, the activation appeared to be complete within a few min (Fig. 2). The formation of the intermedi ...
... pepsin. The intermediate form was relatively stable as compared with pepsinogen, but was gradually converted to pepsin during a long period of incubation. When analyzed by the proteolytic activity assay, the activation appeared to be complete within a few min (Fig. 2). The formation of the intermedi ...
22nd EMC Full Program - 25th Enzyme Mechanisms Conference
... molecular underpinnings of RTK regulation, function, oncogenic activation, and drug selectivity are still not fully understood. Oncogenic receptor tyrosine kinases and clinical agents were studied to further our understanding of this important enzyme class. Receptor tyrosine kinases such as cMet can ...
... molecular underpinnings of RTK regulation, function, oncogenic activation, and drug selectivity are still not fully understood. Oncogenic receptor tyrosine kinases and clinical agents were studied to further our understanding of this important enzyme class. Receptor tyrosine kinases such as cMet can ...
Co-translational Targeting and Translocation of the Amino Terminus
... in these assays, the requirement for ATP could not be independently evaluated. Other in vitro systems have been used to partially dissociate the coupling between translation and translocation across the mammalian ER membrane. However, the targeting and translocation efficiencies were very low (24 – ...
... in these assays, the requirement for ATP could not be independently evaluated. Other in vitro systems have been used to partially dissociate the coupling between translation and translocation across the mammalian ER membrane. However, the targeting and translocation efficiencies were very low (24 – ...
phosphoinositides: tiny lipids with giant impact on cell regulation
... structural fold similar to that of PTB (phosphotyrosine binding) or PH domains (1062, 1416). EHD domains (1120) and BAR domains also bind anionic lipids including inositol lipids and also sense and/or generate membrane curvatures (466). This list is ever expanding and now also includes PDZ domains ( ...
... structural fold similar to that of PTB (phosphotyrosine binding) or PH domains (1062, 1416). EHD domains (1120) and BAR domains also bind anionic lipids including inositol lipids and also sense and/or generate membrane curvatures (466). This list is ever expanding and now also includes PDZ domains ( ...
The Miraculous Benefits of Non Denatured Whey Protein Powder
... You should also then recognize the colossal importance, over and above anything else you can do for your clients, is helping them get ahead of detoxification. With unheated whey protein powder, you will now do a lot more to help your clients achieve 24 hour a day, optimal cellular detoxification th ...
... You should also then recognize the colossal importance, over and above anything else you can do for your clients, is helping them get ahead of detoxification. With unheated whey protein powder, you will now do a lot more to help your clients achieve 24 hour a day, optimal cellular detoxification th ...
InterPro Presentation - European Bioinformatics Institute
... Link related signatures - relationships 1) Parent - Child (subgroup of more closely related proteins) ...
... Link related signatures - relationships 1) Parent - Child (subgroup of more closely related proteins) ...
Glycolytic Enzymes Associate Dynamically with
... regulation of metabolic flux, either in terms of magnitude or direction of flux (Achnine et al., 2004). Such enzyme complexes (metabolons) have been identified in plants, and examples have been characterized from both primary and secondary metabolism (Winkel, 2004; Jorgensen et al., 2005). Although ...
... regulation of metabolic flux, either in terms of magnitude or direction of flux (Achnine et al., 2004). Such enzyme complexes (metabolons) have been identified in plants, and examples have been characterized from both primary and secondary metabolism (Winkel, 2004; Jorgensen et al., 2005). Although ...
Life in the slow lane: molecular mechanisms of estivation
... produce cocoons made of multiple layers of shed skin (Loveridge and Withers, 1981; Pinder et al., 1992). Water loss is also retarded by colligative means by elevating the osmolality of body fluids via the production of high concentrations of solutes. Urea is used for this purpose by various fish, am ...
... produce cocoons made of multiple layers of shed skin (Loveridge and Withers, 1981; Pinder et al., 1992). Water loss is also retarded by colligative means by elevating the osmolality of body fluids via the production of high concentrations of solutes. Urea is used for this purpose by various fish, am ...
The Membrane Steps of Bacterial Cell Wall Synthesis as Antibiotic
... the different domains of MraY. It became clear that MraY has 10 transmembrane domains, and both its N- and C-termini are located in the periplasm across species (Figure 2). Since the precursors for Lipid I are synthesized in the cytoplasm, it follows that the active site of MraY is exposed to the cy ...
... the different domains of MraY. It became clear that MraY has 10 transmembrane domains, and both its N- and C-termini are located in the periplasm across species (Figure 2). Since the precursors for Lipid I are synthesized in the cytoplasm, it follows that the active site of MraY is exposed to the cy ...
Regulators of Ubiquitin Dependent Protein Degradation in the
... Ubiquitin dependent protein degradation is a central mechanism regulating multiple functions in cells. Ubiquitination of target proteins requires an enzyme cascade including ubiquitin ligases (E3s). One group of E3s contains cullins as the core component. Activity of these cullin containing E3s is m ...
... Ubiquitin dependent protein degradation is a central mechanism regulating multiple functions in cells. Ubiquitination of target proteins requires an enzyme cascade including ubiquitin ligases (E3s). One group of E3s contains cullins as the core component. Activity of these cullin containing E3s is m ...
G protein–coupled receptor
G protein–coupled receptors (GPCRs), also known as seven-transmembrane domain receptors, 7TM receptors, heptahelical receptors, serpentine receptor, and G protein–linked receptors (GPLR), constitute a large protein family of receptors that sense molecules outside the cell and activate inside signal transduction pathways and, ultimately, cellular responses. Coupling with G proteins, they are called seven-transmembrane receptors because they pass through the cell membrane seven times.G protein–coupled receptors are found only in eukaryotes, including yeast, choanoflagellates, and animals. The ligands that bind and activate these receptors include light-sensitive compounds, odors, pheromones, hormones, and neurotransmitters, and vary in size from small molecules to peptides to large proteins. G protein–coupled receptors are involved in many diseases, and are also the target of approximately 40% of all modern medicinal drugs. Two of the United States's top five selling drugs (Hydrocodone and Lisinopril) act by targeting a G protein–coupled receptor. The 2012 Nobel Prize in Chemistry was awarded to Brian Kobilka and Robert Lefkowitz for their work that was ""crucial for understanding how G protein–coupled receptors function."". There have been at least seven other Nobel Prizes awarded for some aspect of G protein–mediated signaling.There are two principal signal transduction pathways involving the G protein–coupled receptors: the cAMP signal pathway and the phosphatidylinositol signal pathway. When a ligand binds to the GPCR it causes a conformational change in the GPCR, which allows it to act as a guanine nucleotide exchange factor (GEF). The GPCR can then activate an associated G protein by exchanging its bound GDP for a GTP. The G protein's α subunit, together with the bound GTP, can then dissociate from the β and γ subunits to further affect intracellular signaling proteins or target functional proteins directly depending on the α subunit type (Gαs, Gαi/o, Gαq/11, Gα12/13).