Prokaryotic proteins of antioxidant defense in Trichomonas vaginalis
... Three classes of SODs based on amino acid sequence homology are recognized to this date (Fig. 1) (22,73,104,117): ...
... Three classes of SODs based on amino acid sequence homology are recognized to this date (Fig. 1) (22,73,104,117): ...
Лекция 2. Структура и функция белка
... The principle that sequences of monomeric subunits are information-rich emerges fully in the discussion of nucleic acids in Chapter 12. However, proteins and some polysaccharides are also information-rich molecules. The amino acid sequence is a form of information that directs the folding of the pro ...
... The principle that sequences of monomeric subunits are information-rich emerges fully in the discussion of nucleic acids in Chapter 12. However, proteins and some polysaccharides are also information-rich molecules. The amino acid sequence is a form of information that directs the folding of the pro ...
Nitrate reductase activity in chicory roots
... mechanism as shown by increased sensitivity to magnesium of in vitro NRA. Okadaic acid, a serinethreonine protein phosphatases inhibitor, enhanced the decrease of NRA. Conversely, staurosporine, a serine-threonine protein kinases inhibitor, antagonized the inhibition of NRA. This suggests that excis ...
... mechanism as shown by increased sensitivity to magnesium of in vitro NRA. Okadaic acid, a serinethreonine protein phosphatases inhibitor, enhanced the decrease of NRA. Conversely, staurosporine, a serine-threonine protein kinases inhibitor, antagonized the inhibition of NRA. This suggests that excis ...
Accumulation of xylem transported protein at pit membranes and
... and olive (Olea europaea L.) were shown by confocal laser scanning microscopy to accumulate specifically at wallbased pit membranes that connect neighbouring xylem conduits. In addition, pressure-induced perfusion of microfiltered ovalbumin solutions, at concentrations similar to those of endogenous ...
... and olive (Olea europaea L.) were shown by confocal laser scanning microscopy to accumulate specifically at wallbased pit membranes that connect neighbouring xylem conduits. In addition, pressure-induced perfusion of microfiltered ovalbumin solutions, at concentrations similar to those of endogenous ...
Post-Translational Modifications of the TAK1-TAB Complex
... As shown in Figure 1, receptor-mediated TAK1 activation mostly depends on the E3 ubiquitin ligase activity of TNF-receptor-associated factor (TRAF) 2 or 6 [3,21,22]. Once TNF-α binds to TNF receptor 1 (TNFR1), the adaptor molecule TNFR1-associated DEATH domain protein (TRADD) is recruited to TNFR1, ...
... As shown in Figure 1, receptor-mediated TAK1 activation mostly depends on the E3 ubiquitin ligase activity of TNF-receptor-associated factor (TRAF) 2 or 6 [3,21,22]. Once TNF-α binds to TNF receptor 1 (TNFR1), the adaptor molecule TNFR1-associated DEATH domain protein (TRADD) is recruited to TNFR1, ...
PKC modulates NF-B and AP-1 via mitogen
... of NF-B has been shown to be a necessary event during ischemic PC (25, 48), and activation of AP-1 has been observed after brief ischemia (5). However, the molecular link between the ⑀-isozyme of PKC and ischemia-activated transcription factors has never been established in cardiac cells. We hypoth ...
... of NF-B has been shown to be a necessary event during ischemic PC (25, 48), and activation of AP-1 has been observed after brief ischemia (5). However, the molecular link between the ⑀-isozyme of PKC and ischemia-activated transcription factors has never been established in cardiac cells. We hypoth ...
Supporting document 1 Safety assessment
... DNA sequences from the backbone of the transformation vector, including antibiotic resistance marker genes, were transferred during the transformation event. The csr1-2 gene expression cassette in CV127 is identical in sequence to the transforming plasmid DNA except for three point mutations, one o ...
... DNA sequences from the backbone of the transformation vector, including antibiotic resistance marker genes, were transferred during the transformation event. The csr1-2 gene expression cassette in CV127 is identical in sequence to the transforming plasmid DNA except for three point mutations, one o ...
Cellular and molecular mechanisms of sex differences in renal
... treatment of ARF, it is associated with significant morbidity and mortality. I/R injury also occurs during renal transplantation and leads to reduced allograft survival. Sex differences have been found in I/R injury in many different organs including the kidney. Women have half the mortality of men ...
... treatment of ARF, it is associated with significant morbidity and mortality. I/R injury also occurs during renal transplantation and leads to reduced allograft survival. Sex differences have been found in I/R injury in many different organs including the kidney. Women have half the mortality of men ...
Protein stabilization: a common consequence of mutations
... sequence speci®c nuclear transcription factor, working in association with its obligate partner, Max. Both dimerization with Max through the C-terminal helix ± loop ± helix/leucine zipper (HLH-LZ) domain and sequence-speci®c DNA binding mediated by the adjacent basic region (B) are prerequisites for ...
... sequence speci®c nuclear transcription factor, working in association with its obligate partner, Max. Both dimerization with Max through the C-terminal helix ± loop ± helix/leucine zipper (HLH-LZ) domain and sequence-speci®c DNA binding mediated by the adjacent basic region (B) are prerequisites for ...
Yeast
... We have isolated the cytosolic form of Cu–Zn superoxide dismutase (SOD) from the marine yeast Debaryomyces hansenii. This enzyme has a subunit mass of 18 kDa. The preparation was found to be heterogeneous by IF electrophoresis with two pI ranges: 5·14–4·0 and 1·6–1·8. The enzyme preparation had a re ...
... We have isolated the cytosolic form of Cu–Zn superoxide dismutase (SOD) from the marine yeast Debaryomyces hansenii. This enzyme has a subunit mass of 18 kDa. The preparation was found to be heterogeneous by IF electrophoresis with two pI ranges: 5·14–4·0 and 1·6–1·8. The enzyme preparation had a re ...
Functional analysis of polar amino
... To determine the intracellular localization of wild-type and mutant NHE1 protein in vivo, transfected cells were grown on glass coverslips to 60±70% confluence. The coverslips were washed three times with NaCl/Pi, pH 7.4 and cells were fixed with 4% formaldehyde in NaCl/Pi. Fixation was terminated w ...
... To determine the intracellular localization of wild-type and mutant NHE1 protein in vivo, transfected cells were grown on glass coverslips to 60±70% confluence. The coverslips were washed three times with NaCl/Pi, pH 7.4 and cells were fixed with 4% formaldehyde in NaCl/Pi. Fixation was terminated w ...
Biomarker discovery for psychiatric disorders
... Within the spectrum of anxiety disorders, the facts that they often co-occur (Hettema et al., 2005), and most of them respond to a similar spectrum of pharmacological treatments (Gross and Hen, 2004; Kent et al., 2002) indicates that they share common neurobiological and physiological characteristic ...
... Within the spectrum of anxiety disorders, the facts that they often co-occur (Hettema et al., 2005), and most of them respond to a similar spectrum of pharmacological treatments (Gross and Hen, 2004; Kent et al., 2002) indicates that they share common neurobiological and physiological characteristic ...
Cell lysate and SR-enriched membrane fraction (SR - HAL
... showed that current-voltage relationships had similar conductance of 639, 632 and 640 pS (for Fig.4A, B and C, respectively). These results suggested that all recorded channel activity corresponded to RyRs, however, although the burst and long-lasting modes were observed in channels with and without ...
... showed that current-voltage relationships had similar conductance of 639, 632 and 640 pS (for Fig.4A, B and C, respectively). These results suggested that all recorded channel activity corresponded to RyRs, however, although the burst and long-lasting modes were observed in channels with and without ...
Acquisition, Conservation, and Loss of Dual
... To date, 72 proteins in Arabidopsis have been shown to be dual targeted (Carrie et al., 2009a; Carrie and Small, 2013); however, as many as 500 are predicted to be dual targeted because they contain ambiguous signals (Mitschke et al., 2009). The necessity for dual targeting remains largely unknown, ...
... To date, 72 proteins in Arabidopsis have been shown to be dual targeted (Carrie et al., 2009a; Carrie and Small, 2013); however, as many as 500 are predicted to be dual targeted because they contain ambiguous signals (Mitschke et al., 2009). The necessity for dual targeting remains largely unknown, ...
A chitin-binding domain in a marine bacterial
... was examined by incubating cell extracts with various concentrations of chitin at 37 "C for 4 h, after which 3 M HC1 was added. The mixture was then centrifuged to remove precipitated protein and undegraded chitin. The supernatant containing soluble by-products from the degraded chitin was hydrolyse ...
... was examined by incubating cell extracts with various concentrations of chitin at 37 "C for 4 h, after which 3 M HC1 was added. The mixture was then centrifuged to remove precipitated protein and undegraded chitin. The supernatant containing soluble by-products from the degraded chitin was hydrolyse ...
Structure, mechanism and function of prenyltransferases
... The products of these prenyltransferases have specific chain lengths essential for their biological functions. An intriguing question is how do they achieve product chainlength specificity. In theory, restriction of the size of the enzyme active site should play a major role in determining the chain l ...
... The products of these prenyltransferases have specific chain lengths essential for their biological functions. An intriguing question is how do they achieve product chainlength specificity. In theory, restriction of the size of the enzyme active site should play a major role in determining the chain l ...
Determination of the Correlation between Conductance and Architecture of Disordered ß-amyloid Channels
... neuronal cell membranes disrupts homeostasis and function leading to Alzheimer’s disease. These channels have been shown to be composed of variable numbers of subunits. The nonhomogenous configuration of these subunits in the channels has introduced difficulties in defining set values of ion conduct ...
... neuronal cell membranes disrupts homeostasis and function leading to Alzheimer’s disease. These channels have been shown to be composed of variable numbers of subunits. The nonhomogenous configuration of these subunits in the channels has introduced difficulties in defining set values of ion conduct ...
Endosomal transport of septin mRNA and protein indicates local
... newly synthesized protein at this site. This is due to the fact that translation and maturation of Gfp takes more than 15 minutes (Figure 7F-G). Thus, instead of using translational inhibitors that globally affect translation and interfere with numerous biological processes within the cell we, by th ...
... newly synthesized protein at this site. This is due to the fact that translation and maturation of Gfp takes more than 15 minutes (Figure 7F-G). Thus, instead of using translational inhibitors that globally affect translation and interfere with numerous biological processes within the cell we, by th ...
Activation of DREAM (Downstream Regulatory
... transmission in LID has been associated with altered redistribution and trafficking of D1Rs (16) along with a massive accumulation of postsynaptic density protein 95 (PSD-95) in the synaptic membrane (17). It has been shown that PSD-95 modulates LID in rats and monkeys by its direct interaction with ...
... transmission in LID has been associated with altered redistribution and trafficking of D1Rs (16) along with a massive accumulation of postsynaptic density protein 95 (PSD-95) in the synaptic membrane (17). It has been shown that PSD-95 modulates LID in rats and monkeys by its direct interaction with ...
SBT202 - SibTech, Inc.
... Functional activity in tissue culture: The ability of EGF/Si to bind to and activate EGF receptor was tested in two tissue culture assays: stimulation of EGFR tyrosine autophosphorylation and competition with EGF-toxin fusion protein (SibTech product # SBT076.012) for binding to EGFR expressed on MD ...
... Functional activity in tissue culture: The ability of EGF/Si to bind to and activate EGF receptor was tested in two tissue culture assays: stimulation of EGFR tyrosine autophosphorylation and competition with EGF-toxin fusion protein (SibTech product # SBT076.012) for binding to EGFR expressed on MD ...
Cardosin A Molecular Determinants and Biosynthetic Pathways
... and it allowed to conclude that the protein’s expression did not retrieved any phenotype to the cells or individuals. However, experiments conducted in BY-2 cells revealed to be inconclusive since cardosin A expression in this system is not predictable. The data obtained along this work using severa ...
... and it allowed to conclude that the protein’s expression did not retrieved any phenotype to the cells or individuals. However, experiments conducted in BY-2 cells revealed to be inconclusive since cardosin A expression in this system is not predictable. The data obtained along this work using severa ...
Tetrahymena Contain Two Distinct and Unusual High Mobility Group
... each other or any other macronuclear polypeptide in immunoblotting analyses. As well, LG-1 and LG-2 antibodies do not react with calf thymus, chicken, or yeast HMG proteins. Consistent with these results, a 47 amino-terminal sequence of LG-1 has been determined that shows limited homology to both ca ...
... each other or any other macronuclear polypeptide in immunoblotting analyses. As well, LG-1 and LG-2 antibodies do not react with calf thymus, chicken, or yeast HMG proteins. Consistent with these results, a 47 amino-terminal sequence of LG-1 has been determined that shows limited homology to both ca ...
Intraplastidic Localization of the Enzymesthat Convert Cucumber
... Laboratory of Plant Pigment Biochemistry and Photobiology, 202 ABL, University of Illinois, Urbana, Illinois 61801 these enzymes is more controversial. On the basis of osmotic lysis of crude etiochloroplast preparations accompanied by differential centrifugation, Smith and Rebeiz (27) proposed that ...
... Laboratory of Plant Pigment Biochemistry and Photobiology, 202 ABL, University of Illinois, Urbana, Illinois 61801 these enzymes is more controversial. On the basis of osmotic lysis of crude etiochloroplast preparations accompanied by differential centrifugation, Smith and Rebeiz (27) proposed that ...
Effect of Ca on H -ATPase activity of plasma membrane in
... pump that directly couples ATP hydrolysis with the vectorial transport of H into the appolast. The electrochemical proton gradient acts as a driving force for the secondary transport of ions and nutrients into and out of cell. It also controls some major physiological-biochemical process, e.g. gro ...
... pump that directly couples ATP hydrolysis with the vectorial transport of H into the appolast. The electrochemical proton gradient acts as a driving force for the secondary transport of ions and nutrients into and out of cell. It also controls some major physiological-biochemical process, e.g. gro ...
Selective and differential interactions of BNN27
... Received 18 February 2016 Received in revised form 11 August 2016 Accepted 7 September 2016 Available online 9 September 2016 ...
... Received 18 February 2016 Received in revised form 11 August 2016 Accepted 7 September 2016 Available online 9 September 2016 ...
G protein–coupled receptor
G protein–coupled receptors (GPCRs), also known as seven-transmembrane domain receptors, 7TM receptors, heptahelical receptors, serpentine receptor, and G protein–linked receptors (GPLR), constitute a large protein family of receptors that sense molecules outside the cell and activate inside signal transduction pathways and, ultimately, cellular responses. Coupling with G proteins, they are called seven-transmembrane receptors because they pass through the cell membrane seven times.G protein–coupled receptors are found only in eukaryotes, including yeast, choanoflagellates, and animals. The ligands that bind and activate these receptors include light-sensitive compounds, odors, pheromones, hormones, and neurotransmitters, and vary in size from small molecules to peptides to large proteins. G protein–coupled receptors are involved in many diseases, and are also the target of approximately 40% of all modern medicinal drugs. Two of the United States's top five selling drugs (Hydrocodone and Lisinopril) act by targeting a G protein–coupled receptor. The 2012 Nobel Prize in Chemistry was awarded to Brian Kobilka and Robert Lefkowitz for their work that was ""crucial for understanding how G protein–coupled receptors function."". There have been at least seven other Nobel Prizes awarded for some aspect of G protein–mediated signaling.There are two principal signal transduction pathways involving the G protein–coupled receptors: the cAMP signal pathway and the phosphatidylinositol signal pathway. When a ligand binds to the GPCR it causes a conformational change in the GPCR, which allows it to act as a guanine nucleotide exchange factor (GEF). The GPCR can then activate an associated G protein by exchanging its bound GDP for a GTP. The G protein's α subunit, together with the bound GTP, can then dissociate from the β and γ subunits to further affect intracellular signaling proteins or target functional proteins directly depending on the α subunit type (Gαs, Gαi/o, Gαq/11, Gα12/13).