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Genetic Engineering and Biotechnology
Genetic Engineering and Biotechnology

... The use of living organisms to carry out defined chemical processes for industrial or commercial application. The office of Technology Assessment of the U.S. Congress defines biotechnology as "any technique that uses living organisms or their products to make or modify a product, to improve plants o ...
PowerPoint
PowerPoint

... DNA that can be used for identification  Based on noncoding regions of DNA  Noncoding regions have repeating DNA sequences  Number of repeats differs between people  Banding pattern on a gel is a DNA fingerprint ...
Unit 1 – Biochemisty
Unit 1 – Biochemisty

... I can describe the pH scale and give examples of substances that are acidic and basic What is pH? ________________________________________________ Label the pH Scale with acids, bases, and neutral. Draw arrow to indicate the increasing strength. ...
35. Modeling Recominant DNA
35. Modeling Recominant DNA

... enzymes are used, which can be thought of as DNA scissors. Enzymes occur naturally in organisms, particularly valuable to scientists are restriction enzymes found in bacteria. Each particular enzyme recognizes a specific, short, nucleotide sequence in DNA molecules. The restriction enzyme will cut t ...
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry

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supp-MBS 103-B

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Enzymes/Macromolecules/Bonding

Genetic Engineering
Genetic Engineering

... • Biotechnology: process of manipulating organisms or their components for the purpose of making useful products. ...
Enzymes
Enzymes

... ENZYME SHAPE  Enzymes have specific shapes, which enable them to attach ...
Packet #3
Packet #3

... 5. Recombinant human insulin, produced by bacteria carrying a cloned insulin gene, is now the major form of insulin used to treat diabetes. The human insulin gene encodes an mRNA only 333 nucleotides long, but the entire gene spans more than 4000 nucleotides. There are three exons and two introns. a ...
Document
Document

... Relate the shape of an enzyme to its function Describe how high temperatures affect enzymes Describe how enzymes work at different pH values Describe examples of enzymes that work outside of body cells, such as digestive enzymes, including details of where they are produced, where they go, and what ...
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Syllabus (Principles of Biotechnology) File

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Quiz: DNA, RNA and Protein
Quiz: DNA, RNA and Protein

... 11. What kind of bond holds the DNA bases together? 12. A three nucleotide sequence of DNA is called a _______________. 13. How many different amino acids are there? 14. State three differences between DNA and RNA. 15. The base uracil pairs with what DNA nucleotide 16. If the DNA coding strand is GT ...
An Application of immobilized enzymes Biosensors
An Application of immobilized enzymes Biosensors

... glucose in the gel • Electrodes respond to changes in the oxygen tension • Size of electrical signal proportional to oxygen uptake and thus glucose concentration ...
Recombinant DNA Technology - BLI-Research-Synbio
Recombinant DNA Technology - BLI-Research-Synbio

... • Restriction enzymes cut DNA by cleaving the sugar-phosphate backbone. • Restriction enzymes do not randomly cut, nor do they all cut DNA in the same location. • Like other enzymes they show specificity for certain sites. • Restriction enzymes recognize, bind to, and cut DNA within specific base se ...
Recombinant DNA Technology
Recombinant DNA Technology

... cells copy the vector DNA along with their own DNA, creating multiple copies of the inserted DNA. ...
Genetic Engineering and Recombinant DNA Technology
Genetic Engineering and Recombinant DNA Technology

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video slide - BiologyAlive.com

Project: Create Your Own Enzyme!
Project: Create Your Own Enzyme!

... lipase breaks down lipids, sucrose breaks down sucrose, and lactase breaks down lactose. But enzymes do more than just breaking down molecules. Some enzymes are also required to build molecules. There are many enzymes in ribosomes (such as peptydil synthetase) that are responsible for building new p ...
Enzymes are specific? - The BioUpdate Foundation
Enzymes are specific? - The BioUpdate Foundation

Topic 20 revision notes - Mr Cartlidge`s Saigon Science Blog
Topic 20 revision notes - Mr Cartlidge`s Saigon Science Blog

... the insertion of genes into crop plants to confer resistance to insect pests ...
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I - Decatur ISD

Cloning Restriction Fragments of Cellular DNA
Cloning Restriction Fragments of Cellular DNA

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Restriction enzyme

A restriction enzyme or restriction endonuclease is an enzyme that cuts DNA at or near specific recognition nucleotide sequences known as restriction sites. Restriction enzymes are commonly classified into three types, which differ in their structure and whether they cut their DNA substrate at their recognition site, or if the recognition and cleavage sites are separate from one another. To cut DNA, all restriction enzymes make two incisions, once through each sugar-phosphate backbone (i.e. each strand) of the DNA double helix.These enzymes are found in bacteria and archaea and provide a defense mechanism against invading viruses. Inside a prokaryote, the restriction enzymes selectively cut up foreign DNA in a process called restriction; while host DNA is protected by a modification enzyme (a methyltransferase) that modifies the prokaryotic DNA and blocks cleavage. Together, these two processes form the restriction modification system.Over 3000 restriction enzymes have been studied in detail, and more than 600 of these are available commercially. These enzymes are routinely used for DNA modification in laboratories, and are a vital tool in molecular cloning.
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