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MBMB451A Section1 Fall 2008 KEY These questions may have
... 23. Describe how proteins can recognize and bind to specific DNA sequences. Make sure to highlight the features of the DNA as well as the protein that are important in this intermolecular interaction. (7points) Keywords – DNA sequence recognition ( donor and acceptor sites in neucleotide base and pr ...
... 23. Describe how proteins can recognize and bind to specific DNA sequences. Make sure to highlight the features of the DNA as well as the protein that are important in this intermolecular interaction. (7points) Keywords – DNA sequence recognition ( donor and acceptor sites in neucleotide base and pr ...
DNA, Proteins, and Biotechnology
... State that, in gel electrophoresis, fragments of DNA move in an electric field and are separated according to their size. State that gel electrophoresis of DNA is used in DNA profiling. Describe the application of DNA profiling to determine paternity and also in forensic investigations. Analyse DNA ...
... State that, in gel electrophoresis, fragments of DNA move in an electric field and are separated according to their size. State that gel electrophoresis of DNA is used in DNA profiling. Describe the application of DNA profiling to determine paternity and also in forensic investigations. Analyse DNA ...
Name: Chapter 8 DNA Study Guide There are two main nucleic
... 6. The ____________ is composed of one atom of phosphorus surrounded by four oxygen atoms 7. A _______________ is a carbon ring structure that contains one or more atoms of nitrogen. 8. In DNA, there are four possible nitrogenous bases: 1. A 2. T 3. G 4. C 9. Nucleotides join together to form long c ...
... 6. The ____________ is composed of one atom of phosphorus surrounded by four oxygen atoms 7. A _______________ is a carbon ring structure that contains one or more atoms of nitrogen. 8. In DNA, there are four possible nitrogenous bases: 1. A 2. T 3. G 4. C 9. Nucleotides join together to form long c ...
Study Guide MBMB 451A Fall 2002
... Nucleic Acid Structures and Manipulation 1. Know nucleotide and nucleoside structure and nomenclature. 2. What are the forces that help stabilize DNA double helical structure? 3. What is Tm? 4. What are the differences between A, B, and Z DNA? What conditions and sequence favor the formation of Z DN ...
... Nucleic Acid Structures and Manipulation 1. Know nucleotide and nucleoside structure and nomenclature. 2. What are the forces that help stabilize DNA double helical structure? 3. What is Tm? 4. What are the differences between A, B, and Z DNA? What conditions and sequence favor the formation of Z DN ...
Enzymes - WordPress.com
... DO NOT allow ‘same’ shape! DO NOT allow active site is on the substrate ...
... DO NOT allow ‘same’ shape! DO NOT allow active site is on the substrate ...
Recombinant DNA technology DNA Isolation and Purification
... The ability to isolate, separate, and visualize DNA fragments would be useless unless some method was available to cut the DNA into fragments of different sizes. In fact, naturally occurring restriction enzymes or restriction endonucleases are the key to making DNA fragments. These bacterial enzymes ...
... The ability to isolate, separate, and visualize DNA fragments would be useless unless some method was available to cut the DNA into fragments of different sizes. In fact, naturally occurring restriction enzymes or restriction endonucleases are the key to making DNA fragments. These bacterial enzymes ...
Biotechnology
... Restriction enzymes cut DNA. • Different restriction enzymes cut DNA in different ways. – each enzyme has a different restriction site – some cut straight across and leave “blunt ends” – some make staggered cuts and leave “sticky ends” ...
... Restriction enzymes cut DNA. • Different restriction enzymes cut DNA in different ways. – each enzyme has a different restriction site – some cut straight across and leave “blunt ends” – some make staggered cuts and leave “sticky ends” ...
Southern Blotting DNA Fingerprinting
... Southern Blot • A Southern Blot identifies specific sequences of DNA • A Southern Blot may be used to determine a DNA fingerprint • A Southern Blot may be used in forsenic medicine ...
... Southern Blot • A Southern Blot identifies specific sequences of DNA • A Southern Blot may be used to determine a DNA fingerprint • A Southern Blot may be used in forsenic medicine ...
Sequencing a genome - Information Services and Technology
... independently of the chromosomes; artificial plasmids can be inserted into bacteria to amplify DNA for sequencing ...
... independently of the chromosomes; artificial plasmids can be inserted into bacteria to amplify DNA for sequencing ...
Replication of the DNA
... 1) Type I restriction enzyme – Cut the DNA a thousand or more bases pair away from its recognition sequence – The base sequence at the cut site is not fixed. – These enzymes are suicidal. – So, not of much use to molecular biologists ...
... 1) Type I restriction enzyme – Cut the DNA a thousand or more bases pair away from its recognition sequence – The base sequence at the cut site is not fixed. – These enzymes are suicidal. – So, not of much use to molecular biologists ...
Enzymes - Westgate Mennonite Collegiate
... Our body needs to digest them – turn them into a form that can be absorbed into the blood and used by cells. Enzymes make this possible. Enzyme video/animation: https://www.youtube.com/watch?v=r1ryDVgx0zw ...
... Our body needs to digest them – turn them into a form that can be absorbed into the blood and used by cells. Enzymes make this possible. Enzyme video/animation: https://www.youtube.com/watch?v=r1ryDVgx0zw ...
MutaGEL® r-Vitamin D3
... The kit MutaGEL r-vitamin D3 contains a set of primer for amplification of the specific DNA sequence within the human vitamin D3 receptor gene VD3R. Amplificates of variing genotypes (start codon polymorphism) are characterized by subsequent specific restriction enzyme digestion. The rare variant (f ...
... The kit MutaGEL r-vitamin D3 contains a set of primer for amplification of the specific DNA sequence within the human vitamin D3 receptor gene VD3R. Amplificates of variing genotypes (start codon polymorphism) are characterized by subsequent specific restriction enzyme digestion. The rare variant (f ...
Enzymes
... ENZYMES ARE SPECIFIC • Every enzyme can only be used for one reaction. Each one can only bond with one substrate • So every time you have a new substrate, you need a new enzyme • This is called being SUBSTRATE SPECIFIC ...
... ENZYMES ARE SPECIFIC • Every enzyme can only be used for one reaction. Each one can only bond with one substrate • So every time you have a new substrate, you need a new enzyme • This is called being SUBSTRATE SPECIFIC ...
12711_2011_2534_MOESM1_ESM
... Blank extractions and several negative PCR controls should be performed alongside extractions and amplifications from ancient material. In fact, the quantity of DNA 2 contamination present in laboratory reagents may be so small that it is detected only sporadically in negative controls. Repeated amp ...
... Blank extractions and several negative PCR controls should be performed alongside extractions and amplifications from ancient material. In fact, the quantity of DNA 2 contamination present in laboratory reagents may be so small that it is detected only sporadically in negative controls. Repeated amp ...