Biochemistry - cloudfront.net
... Lipids tend to be the largest of the organic molecules. They carry more energy then carbohydrates, but they are not utilized to make ATP during respiration like glucose because they are too difficult and too large to ...
... Lipids tend to be the largest of the organic molecules. They carry more energy then carbohydrates, but they are not utilized to make ATP during respiration like glucose because they are too difficult and too large to ...
CHAPTER-II ENZYMES
... mechanism. Cofactors Some enzymes do not need any additional components to show full activity. However, others require non-protein molecules called cofactors to be bound for activity. Cofactors can be either inorganic (e.g., metal ions and iron-sulfur clusters) or organic compounds (e.g., flavin and ...
... mechanism. Cofactors Some enzymes do not need any additional components to show full activity. However, others require non-protein molecules called cofactors to be bound for activity. Cofactors can be either inorganic (e.g., metal ions and iron-sulfur clusters) or organic compounds (e.g., flavin and ...
大碩102研究所全真模擬考試試題
... (B) Telomerase is a unique enzyme in that it is composed of only RNA. (C) Without telomeres, linear eukaryotic chromosomes would get shorter and shorter with each round of DNA replication (D) All organisms must protect their telomeres from nucleases and double strand break repair enzymes. 38. EF-Tu ...
... (B) Telomerase is a unique enzyme in that it is composed of only RNA. (C) Without telomeres, linear eukaryotic chromosomes would get shorter and shorter with each round of DNA replication (D) All organisms must protect their telomeres from nucleases and double strand break repair enzymes. 38. EF-Tu ...
Principles of Enzyme Catalysis\Principles
... contributions. The identities of the contributions depend on the nature of the reaction catalyzed by the enzyme and the cellular environment (e.g., pH, ionic strength, ion composition, and organelle among others) in which the reaction occurs. A. Since all enzyme-catalyzed reactions proceed through d ...
... contributions. The identities of the contributions depend on the nature of the reaction catalyzed by the enzyme and the cellular environment (e.g., pH, ionic strength, ion composition, and organelle among others) in which the reaction occurs. A. Since all enzyme-catalyzed reactions proceed through d ...
Principles of Enzyme Catalysis\Principles of Enzyme Catalysis.wpd
... microenvironment of lower dielectric constant than bulk solution. This effect can be further enhanced by substrate binding induced conformational changes in the protein to engulf the bound substrate. The resulting hydrophobic microenvironment enhances the reactivity of nucleophiles and electrophiles ...
... microenvironment of lower dielectric constant than bulk solution. This effect can be further enhanced by substrate binding induced conformational changes in the protein to engulf the bound substrate. The resulting hydrophobic microenvironment enhances the reactivity of nucleophiles and electrophiles ...
4C-Seq primer database Tanay, deLaat Groups Version 0.7, July
... DpnII-BfaI, DpnII-Csp6I, NlaIII-BfaI, NlaIII-Csp6I, and NlaIII-DpnII. 2. Primer design 4C primer pairs carry additional 5’ overhangs composed of the adapter sequences (obtained from Illumina technical support) necessary for Illumina single read sequencing (GA-II and Hi-seq 2000). The strategy theref ...
... DpnII-BfaI, DpnII-Csp6I, NlaIII-BfaI, NlaIII-Csp6I, and NlaIII-DpnII. 2. Primer design 4C primer pairs carry additional 5’ overhangs composed of the adapter sequences (obtained from Illumina technical support) necessary for Illumina single read sequencing (GA-II and Hi-seq 2000). The strategy theref ...
7.02 Fall 2001 Recombinant DNA methods Agenda
... • On Day 3, we will isolate genomic DNA from each of these two strains using a boiling lysis method. Then we’ll use specific pairs of PCR primers to selectively amplify three regions of the Ara operon, corresponding to the A, B, and C genes. The results of this analysis will tell us which of these t ...
... • On Day 3, we will isolate genomic DNA from each of these two strains using a boiling lysis method. Then we’ll use specific pairs of PCR primers to selectively amplify three regions of the Ara operon, corresponding to the A, B, and C genes. The results of this analysis will tell us which of these t ...
L 17 _PCR
... B. Site-directed mutagenesis. By altering the primer sequences, we can generate PCR products that are mutated in defined ways. Useful in analysis of gene and protein function. C. DNA fingerprinting is used in forensics, determining parentage, etc. VNTRs (variable number tandem repeats) or SSLPs (sim ...
... B. Site-directed mutagenesis. By altering the primer sequences, we can generate PCR products that are mutated in defined ways. Useful in analysis of gene and protein function. C. DNA fingerprinting is used in forensics, determining parentage, etc. VNTRs (variable number tandem repeats) or SSLPs (sim ...
Extraction of Plasmid DNA, Restriction Digest, and DNA Gel
... cells makes large amounts of protein X based on the sequence of the gene. This is called overexpression. The cornerstone of this technology is the plasmid. Plasmids are circular, small, extrachromosomal, autonomously replicating bacterial DNA molecules that have become indispensable tools in recombi ...
... cells makes large amounts of protein X based on the sequence of the gene. This is called overexpression. The cornerstone of this technology is the plasmid. Plasmids are circular, small, extrachromosomal, autonomously replicating bacterial DNA molecules that have become indispensable tools in recombi ...
DNA damage, repair and recombination
... length and comprise a transposase gene flanked by short (~20 bp) inverted terminal repeats (identical sequences but with opposite orientation). The transposase makes a staggered cut in the chromosomal DNA and, in a replicative process, a copy of the transposon inserts at the target site The gaps are ...
... length and comprise a transposase gene flanked by short (~20 bp) inverted terminal repeats (identical sequences but with opposite orientation). The transposase makes a staggered cut in the chromosomal DNA and, in a replicative process, a copy of the transposon inserts at the target site The gaps are ...
Enzymes
... Describe, with the aid of diagrams, the mechanism of action of enzyme molecules, with reference to specificity, active site, lock and key hypothesis, induced-fit hypothesis, enzyme-substrate complex, enzyme product complex and lowering of activation energy: In a reaction catalysed by an enzyme, the ...
... Describe, with the aid of diagrams, the mechanism of action of enzyme molecules, with reference to specificity, active site, lock and key hypothesis, induced-fit hypothesis, enzyme-substrate complex, enzyme product complex and lowering of activation energy: In a reaction catalysed by an enzyme, the ...
Genes and DNA2012
... “miracle mineral” and was used in conjunction with the growth in industry. Using this readily available and inexpensive material in all manners of household construction offered many benefits, with no visible drawbacks, at the time. Asbestos could be used to fill spaces, cover items, mix with liquid ...
... “miracle mineral” and was used in conjunction with the growth in industry. Using this readily available and inexpensive material in all manners of household construction offered many benefits, with no visible drawbacks, at the time. Asbestos could be used to fill spaces, cover items, mix with liquid ...
The Michaelis-Menten equation
... Enzyme inhibitors are substances which alter the catalytic action of the enzyme and consequently slow down, or in some cases, stop catalysis. Inhibitors may act combining directly with the enzyme and so effectively remove it from the substrate (like Drugs: Heat, pH changes, strong acids, alcohol & a ...
... Enzyme inhibitors are substances which alter the catalytic action of the enzyme and consequently slow down, or in some cases, stop catalysis. Inhibitors may act combining directly with the enzyme and so effectively remove it from the substrate (like Drugs: Heat, pH changes, strong acids, alcohol & a ...
Designing Minor Groove Binding Drugs
... Transcription or replication of DNA only occurs after a signal has been received, usually in the form of a protein that binds to a particular region of the DNA. If a small artificial protein can be developed that would mimic the binding strength and specificity of the natural regulatory protein, the ...
... Transcription or replication of DNA only occurs after a signal has been received, usually in the form of a protein that binds to a particular region of the DNA. If a small artificial protein can be developed that would mimic the binding strength and specificity of the natural regulatory protein, the ...
What are enzymes?
... enzymes are added to an industrial process. If you are using chemicals as a catalyst, you have to put up with a bundle of side effects because chemicals are non-specific. The chemicals will do their thing to whatever they come across. When an enzyme does the job, there are no side effects. For insta ...
... enzymes are added to an industrial process. If you are using chemicals as a catalyst, you have to put up with a bundle of side effects because chemicals are non-specific. The chemicals will do their thing to whatever they come across. When an enzyme does the job, there are no side effects. For insta ...
DNA - KSUMSC
... The chromosomes of many bacteria and viruses contain circular DNA which is supercoiled ...
... The chromosomes of many bacteria and viruses contain circular DNA which is supercoiled ...