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DNA – Structure and Replication
DNA – Structure and Replication

... shallow tray and allowed to set The mixture of DNA is loaded into ‘wells’ at the top end (negative end) of the gel and an electric current is passed through the gel DNA is a negatively charged molecule and will be attracted towards the positive end The large restriction fragments will move more slow ...
File
File

Enzyme - Northwest ISD Moodle
Enzyme - Northwest ISD Moodle

Chapter 4
Chapter 4

2. Enzymes
2. Enzymes

Recombinant DNA
Recombinant DNA

What is DNA Computing?
What is DNA Computing?

... chemically interact according to defined rules to produce new molecules Laboratory techniques that allow the isolation/identification of product molecules with specific properties  PCR, Ligation, Gel Electrophoresis, etc. ...
Snímek 1
Snímek 1

DNA Isolation for Low-Melting Point Agarose (using elu
DNA Isolation for Low-Melting Point Agarose (using elu

GI Digest - Douglas Labs
GI Digest - Douglas Labs

... by pepsin and hydrochloric acid, which denature and break large proteins down to smaller polypeptides. In the small intestine, proteases break down these polypeptides into free amino acids, and di- and tripeptides, which are directly absorbed by the intestinal mucosa. Some individuals require enzyme ...
Biology STAAR EOC Review Sheets Alief
Biology STAAR EOC Review Sheets Alief

... food) and chemically by water, acid, and enzymes in the digestive system; nutrients are then absorbed by blood in the circulatory system Certain hormones produced in the endocrine system control ovulation in a female’s ...
recombinant DNA
recombinant DNA

Genetic engineering
Genetic engineering

enzymes - BEHS Science
enzymes - BEHS Science

... Some enzymes need an “add on” to function  Cofactors (inorganic): Zn, Fe, Cu  Coenzymes (organic) – vitamins B1 B12 B6, C, D ...
do not
do not

... • Enzymes must be made of something that can take many different shapes • Proteins and their 4 levels of structure work well ...
do not - wwphs
do not - wwphs

Ascona B-DNA Consortium
Ascona B-DNA Consortium

Replication and Protein Synthesis Test
Replication and Protein Synthesis Test

... at a deoxyribose sugar and ends at a phosphate group. This strand a. is the coding strand. b. is the template strand. c. runs in the 3’ to 5’ direction. d. runs in the 5’ to 3’ direction. e. is unlikely to be transcribed into RNA. The two strands of a DNA molecule are held together by a. covalent bo ...
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File

Document
Document

Characterisation of DNA by Agarose Gel Electrophoresis and
Characterisation of DNA by Agarose Gel Electrophoresis and

... both, the passing on of information to a new cell during mitosis as well as code for anabolic and catabolic processes within the cell. From the fact alone that information about very complex patterns of life are preserved in the DNA one may deduct that deoxyribonucleic acids are structures of high m ...
B. Enzymes have four features
B. Enzymes have four features

Old First Exam with answer key
Old First Exam with answer key

... have the linear DNA ready in 1 hour You have the option to use any ONE of the following enzymes: EcoRI, SalI, XhoI or SapI. A. Which enzyme would be the best to use? SapI B. Why would you select this enzyme? You need an enzyme that is efficient at cutting supercoiled DNA. NEB page 310 chart shows th ...
HIV-1 Reverse Transcriptase
HIV-1 Reverse Transcriptase

... HIV is a member of the retrovirus family. An essential element in the life cycle of this family of viruses is the requirement for integrating a copy of its genetic material (genome) into the human host cell genome before virus replication can occur. HIV Reverse Transcriptase enzyme (RT) is responsib ...
Market America Intranet
Market America Intranet

< 1 ... 61 62 63 64 65 66 67 68 69 ... 101 >

Restriction enzyme

A restriction enzyme or restriction endonuclease is an enzyme that cuts DNA at or near specific recognition nucleotide sequences known as restriction sites. Restriction enzymes are commonly classified into three types, which differ in their structure and whether they cut their DNA substrate at their recognition site, or if the recognition and cleavage sites are separate from one another. To cut DNA, all restriction enzymes make two incisions, once through each sugar-phosphate backbone (i.e. each strand) of the DNA double helix.These enzymes are found in bacteria and archaea and provide a defense mechanism against invading viruses. Inside a prokaryote, the restriction enzymes selectively cut up foreign DNA in a process called restriction; while host DNA is protected by a modification enzyme (a methyltransferase) that modifies the prokaryotic DNA and blocks cleavage. Together, these two processes form the restriction modification system.Over 3000 restriction enzymes have been studied in detail, and more than 600 of these are available commercially. These enzymes are routinely used for DNA modification in laboratories, and are a vital tool in molecular cloning.
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