5.2.3 Genomes and Gene Technology MS
... cut DNA with restriction enzyme; ref to sticky ends; complementary; base pairs / CCC and GGG / C pairing with G / alternative; (DNA) ligase / ligation; ref to bonding / AW; e.g. hydrogen or phosphodiester / sugar-phosphate AVP; e.g. add sticky ends to blunt ends cut both at the same place ...
... cut DNA with restriction enzyme; ref to sticky ends; complementary; base pairs / CCC and GGG / C pairing with G / alternative; (DNA) ligase / ligation; ref to bonding / AW; e.g. hydrogen or phosphodiester / sugar-phosphate AVP; e.g. add sticky ends to blunt ends cut both at the same place ...
1. Most organisms are active in a limited temperature range
... chains fold in a specific way forming active sites • Many enzymes require the presence of other factors as well as the protein part before they act. These non-protein parts are called cofactors and include metallic ions like iron, calcium, copper and zinc. If the cofactor is an organic molecule like ...
... chains fold in a specific way forming active sites • Many enzymes require the presence of other factors as well as the protein part before they act. These non-protein parts are called cofactors and include metallic ions like iron, calcium, copper and zinc. If the cofactor is an organic molecule like ...
Tutorial - Faster Better Media
... Note that SB™ (lanes 1 and 2) and LB™ (lane 9) are excellent for small DNA but encounter crowding of the bands of larger DNA when run in standard agarose (lanes 1 and 5), possibly due to intramolecular DNA crosslinking, which reduces the discriminating shape differences among the larger molecules. T ...
... Note that SB™ (lanes 1 and 2) and LB™ (lane 9) are excellent for small DNA but encounter crowding of the bands of larger DNA when run in standard agarose (lanes 1 and 5), possibly due to intramolecular DNA crosslinking, which reduces the discriminating shape differences among the larger molecules. T ...
molecular biology
... Sub classes of type II Restriction endonucleases Based on the recognition sequence and cleavage site, the type II REs can further be divided into following sub classes. P - Palindromic recognition sequence A - Asymmetrical recognition sequence B - Cleavage takes place on both sides of the recognitio ...
... Sub classes of type II Restriction endonucleases Based on the recognition sequence and cleavage site, the type II REs can further be divided into following sub classes. P - Palindromic recognition sequence A - Asymmetrical recognition sequence B - Cleavage takes place on both sides of the recognitio ...
4-BCH201_Enzymes
... Some enzymes are synthesized in active form and require no chemical groups for activity other than their amino acid residues. Other enzymes are produced in an inactive form due to either: - Presence of excess polypeptide in their structure and is converted to active form after deletion of this part. ...
... Some enzymes are synthesized in active form and require no chemical groups for activity other than their amino acid residues. Other enzymes are produced in an inactive form due to either: - Presence of excess polypeptide in their structure and is converted to active form after deletion of this part. ...
Rate Law in Enzyme Catalyzed Reactions
... synthesized in an inactive conformation, then activated by proteolytic cleavage What is an isozyme? (1) Isozymes are physically distinct forms of the same enzyme. (2) Isozymes may differ from each other by differences in their amino acid sequences or by the presence of different posttranslational mo ...
... synthesized in an inactive conformation, then activated by proteolytic cleavage What is an isozyme? (1) Isozymes are physically distinct forms of the same enzyme. (2) Isozymes may differ from each other by differences in their amino acid sequences or by the presence of different posttranslational mo ...
Questions for Enzyme - I
... b. Never altered by enzyme inhibitors c. It is the conc. Of enzyme at half maximal velocity d. One enzyme can have different Km values with same substrate ...
... b. Never altered by enzyme inhibitors c. It is the conc. Of enzyme at half maximal velocity d. One enzyme can have different Km values with same substrate ...
Design of gRNA and construction of gRNA expression vectors
... binding would not inhibit transcription or disrupt nucleosome positioning. In contrast, for identification of binding molecules of genomic regions with distinct boundaries such as enhancer or silencer, the binding site of gRNA can directly be juxtaposed to the regions because of less probability of ...
... binding would not inhibit transcription or disrupt nucleosome positioning. In contrast, for identification of binding molecules of genomic regions with distinct boundaries such as enhancer or silencer, the binding site of gRNA can directly be juxtaposed to the regions because of less probability of ...
CS5238: Combinatorial Methods in Computation
... miRNA is encoded as a non-coding RNA. It first transcribed as a primary transcript called primary miRNA (pri-miRNA). It then cleaved into a precursor miRNA (premiRNA) with the help of the nuclease Drosha. Precursor miRNA is of length ~6080 nt and can potentially fold into a stemloop structure. The p ...
... miRNA is encoded as a non-coding RNA. It first transcribed as a primary transcript called primary miRNA (pri-miRNA). It then cleaved into a precursor miRNA (premiRNA) with the help of the nuclease Drosha. Precursor miRNA is of length ~6080 nt and can potentially fold into a stemloop structure. The p ...
pdf - NUS Computing
... miRNA is encoded as a non-coding RNA. It first transcribed as a primary transcript called primary miRNA (pri-miRNA). It then cleaved into a precursor miRNA (premiRNA) with the help of the nuclease Drosha. Precursor miRNA is of length ~6080 nt and can potentially fold into a stemloop structure. The p ...
... miRNA is encoded as a non-coding RNA. It first transcribed as a primary transcript called primary miRNA (pri-miRNA). It then cleaved into a precursor miRNA (premiRNA) with the help of the nuclease Drosha. Precursor miRNA is of length ~6080 nt and can potentially fold into a stemloop structure. The p ...
ENZYMES: CLASSIFICATION, STRUCTURE
... Koshland theory (induced-fit model) The process of substrate binding induces specific conformational changes in the the active site region ...
... Koshland theory (induced-fit model) The process of substrate binding induces specific conformational changes in the the active site region ...
File
... Some were left to replicate just once (50 minutes) and others left long enough for just two, three and four times of replication ...
... Some were left to replicate just once (50 minutes) and others left long enough for just two, three and four times of replication ...
Taq Polymerase - cloudfront.net
... copied from the DNA. As the anticodons and codons match up, the amino acids break away in a polypeptide chain. http://www.biologycorner.com/bio2/gene tics/notes_transcription_translation.html ...
... copied from the DNA. As the anticodons and codons match up, the amino acids break away in a polypeptide chain. http://www.biologycorner.com/bio2/gene tics/notes_transcription_translation.html ...
Chapter 3
... – Nylon or nitrocellulose filter is placed over the plate and some of the bacterial colonies stick to the filter at the exact location they were on the plate – Treat filter with alkaline solution to lyse the cells and denature the DNA – Denatured DNA binds to filter as single-stranded DNA – Filter i ...
... – Nylon or nitrocellulose filter is placed over the plate and some of the bacterial colonies stick to the filter at the exact location they were on the plate – Treat filter with alkaline solution to lyse the cells and denature the DNA – Denatured DNA binds to filter as single-stranded DNA – Filter i ...
Lecture Chpt. 18 I Virus
... 1.Insert their DNA into host/ the host produces mRNA compliment 2.Production of new viral proteins, or join with the DNA of the host cell 3.Directs production of new viruses ...
... 1.Insert their DNA into host/ the host produces mRNA compliment 2.Production of new viral proteins, or join with the DNA of the host cell 3.Directs production of new viruses ...
7.6 Enzymes – summary of mark schemes
... end-product can inhibit enzyme needed for early / first step in metabolic pathway; negative feedback since increased level of product decreases rate of its own production; metabolic pathway regulated according to the requirement for its end-product; idea that inhibition is reversible; ...
... end-product can inhibit enzyme needed for early / first step in metabolic pathway; negative feedback since increased level of product decreases rate of its own production; metabolic pathway regulated according to the requirement for its end-product; idea that inhibition is reversible; ...
Enzymes - SBI4UAssumption
... Some enzymes require co-factors or co-enzymes to help them function properly. Co-factors are inorganic, non protein molecules that include zinc, iron, and copper. These metal ions can either bind to the active site or to the substrate to facilitate the formation of the enzyme-substrate complex. ...
... Some enzymes require co-factors or co-enzymes to help them function properly. Co-factors are inorganic, non protein molecules that include zinc, iron, and copper. These metal ions can either bind to the active site or to the substrate to facilitate the formation of the enzyme-substrate complex. ...