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INTRODUCTION TO BACTERIAL GENETICS
... for carrying foreign genes into bacteria, so E. coli was an obvious choice as an experimental organism to Cohen and Boyer. Plasmids are small double-stranded circles of DNA, which are carried by some bacteria in addition to their larger circular genome. In nature, plasmids carry extra information no ...
... for carrying foreign genes into bacteria, so E. coli was an obvious choice as an experimental organism to Cohen and Boyer. Plasmids are small double-stranded circles of DNA, which are carried by some bacteria in addition to their larger circular genome. In nature, plasmids carry extra information no ...
PPT
... for 10~20 min at RT Fragments of 10~50 bp were purified from 2% low meltin point agarose gels ...
... for 10~20 min at RT Fragments of 10~50 bp were purified from 2% low meltin point agarose gels ...
Lecture 26: Overview of deoxyribonucleic acid (DNA) and
... left helices the purine residues flip to the syn conformation alternating with pyrimidines in the anti conformation. Some Unusual structures Bends occur in DNA helix wherever four or more adenosine residues appear sequentially in one strand. This bending may be important in binding of some proteins ...
... left helices the purine residues flip to the syn conformation alternating with pyrimidines in the anti conformation. Some Unusual structures Bends occur in DNA helix wherever four or more adenosine residues appear sequentially in one strand. This bending may be important in binding of some proteins ...
Proteins and Enzymes (p
... where the necessary enzymes are clustered and isolated. The enzymes are determined by the cell’s genetic make-up. ...
... where the necessary enzymes are clustered and isolated. The enzymes are determined by the cell’s genetic make-up. ...
DNA replication and PCR ppt
... axis to form a double helix For each chain – the backbone comes from linking the pentose sugar bases between nucleotides via phosphodiester bonds connecting via 3’ to 5’ The bases face inward and pair in a highly specific fashion with bases in the other chain A only with T, G only with C Bec ...
... axis to form a double helix For each chain – the backbone comes from linking the pentose sugar bases between nucleotides via phosphodiester bonds connecting via 3’ to 5’ The bases face inward and pair in a highly specific fashion with bases in the other chain A only with T, G only with C Bec ...
Document
... The rates of enzyme-catalyzed reactions are altered by activators and inhibitors (a.k.a. effector molecules). (1) Allosteric enzymes: have more than one site, where effector binding at one site induces a conformational change in the enzyme, altering its affinity for a substrate. An allosteric activa ...
... The rates of enzyme-catalyzed reactions are altered by activators and inhibitors (a.k.a. effector molecules). (1) Allosteric enzymes: have more than one site, where effector binding at one site induces a conformational change in the enzyme, altering its affinity for a substrate. An allosteric activa ...
Practical 1
... GGU AUG AUA UAC CGG UAA GGA UGA AAU AUC UCU CAG CCC UGC AUA CUU ACC UUU GAA AAA ACU GUC UUG UUU CUU UAA "G" "M" "I" "Y" "R" "*" "G" "*" "N" "I" "S" "Q" "P" "C" "I" "L" "T" "F" "E" "K" "T" "V" "L" "F" "L" "*" GUA UGA UAU ACC GGU AAG GAU GAA AUA UCU CUC AGC CCU GCA UAC UUA CCU UUG AAA AAA CUG UCU UGU ...
... GGU AUG AUA UAC CGG UAA GGA UGA AAU AUC UCU CAG CCC UGC AUA CUU ACC UUU GAA AAA ACU GUC UUG UUU CUU UAA "G" "M" "I" "Y" "R" "*" "G" "*" "N" "I" "S" "Q" "P" "C" "I" "L" "T" "F" "E" "K" "T" "V" "L" "F" "L" "*" GUA UGA UAU ACC GGU AAG GAU GAA AUA UCU CUC AGC CCU GCA UAC UUA CCU UUG AAA AAA CUG UCU UGU ...
Co-enzyme
... • Enzymes are substrate specific – The substrate fits like a key in a lock – When the substrate and enzyme are joined the catalytic action of the enzyme converts the substrate to product • The specificity of an enzyme for a substrate results from the shape of the enzyme –due to a compatible fit ...
... • Enzymes are substrate specific – The substrate fits like a key in a lock – When the substrate and enzyme are joined the catalytic action of the enzyme converts the substrate to product • The specificity of an enzyme for a substrate results from the shape of the enzyme –due to a compatible fit ...
Micro Lab Unit 1 Flashcards
... 18) What is the result of an enzymatic reaction? 19) What happens to the enzyme after it reacts with the substrate? 20) What is the name of the optimum set of conditions that produce the most efficient enzymatic activity? 21) What might enzymes require in order to function properly 22) What are cofa ...
... 18) What is the result of an enzymatic reaction? 19) What happens to the enzyme after it reacts with the substrate? 20) What is the name of the optimum set of conditions that produce the most efficient enzymatic activity? 21) What might enzymes require in order to function properly 22) What are cofa ...
Lec 19
... lowest. The phages are said to be restricted by the second host strain (E.coli K) and the phenomenon is called restriction. When those phage that do result from the infection of E. coli K are now replated on E. coli K they are no longer restricted; but if they are first cycled through E. coli C they ...
... lowest. The phages are said to be restricted by the second host strain (E.coli K) and the phenomenon is called restriction. When those phage that do result from the infection of E. coli K are now replated on E. coli K they are no longer restricted; but if they are first cycled through E. coli C they ...
Gene Cloning, Expression, and Substrate Specificity of an Imidase
... In general, they are dimer or tetramer with a 50- to 60kD subunit [4, 5], with the exception of two imidases being reported so far as having a lower subunit molecular mass of 30 to 40 kDa. Contrary to known imidase, ...
... In general, they are dimer or tetramer with a 50- to 60kD subunit [4, 5], with the exception of two imidases being reported so far as having a lower subunit molecular mass of 30 to 40 kDa. Contrary to known imidase, ...
Sources of enzyme
... enzymes may be more efficient than sequential catalysis by a separated series of the enzymes. such enzyme preparations are kinetically more simple than the integrated living organisms from which they are produced ...
... enzymes may be more efficient than sequential catalysis by a separated series of the enzymes. such enzyme preparations are kinetically more simple than the integrated living organisms from which they are produced ...
Chem 465 Biochemistry II Hour Exam 2
... D) represents over 90% of the DNA polymerase activity in E. coli cells. E) requires a free 5'-hydroxyl group as a primer. ...
... D) represents over 90% of the DNA polymerase activity in E. coli cells. E) requires a free 5'-hydroxyl group as a primer. ...
Bacterial enzymes that can deglycate glucose
... that this enzyme and the operon that controls the metabolism of these modified amino acids are found in bacteria associated with the gastrointestinal tract or gastrointestinal tract infections, such as the Gram-positive Enterococcus, and the Gram-negative E. coli, Salmonella and Shigella. The precis ...
... that this enzyme and the operon that controls the metabolism of these modified amino acids are found in bacteria associated with the gastrointestinal tract or gastrointestinal tract infections, such as the Gram-positive Enterococcus, and the Gram-negative E. coli, Salmonella and Shigella. The precis ...