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The DNA sequence of the gene and genetic control sites for the
The DNA sequence of the gene and genetic control sites for the

... There are several different forms of RNA polymerase in 13. subtilis which differ in a factor and recognise different classes of promoter sequences (1,2,6). The main vegetative RNA polymerase has a a factor of M r = 55,000 and a promoters are the best characterised in B.subtilis. The consensus sequen ...
Gene Set Testing
Gene Set Testing

... molecular functions (MF) ...
Supplementary Text Comparisons of X and autosomal expression
Supplementary Text Comparisons of X and autosomal expression

... upregulation, given that these genes may have no function and hence low expression levels in somatic tissues [1]. This issue is extensively treated in our analyses that are presented in the main text. ...
Phylogenetics Topic 2: Phylogenetic and genealogical homology
Phylogenetics Topic 2: Phylogenetic and genealogical homology

... two DNA sequences. In such cases they are referring to the percentage of nucleotide sites that are the same between the two sequences. There is considerable potential for misunderstanding because they are usually willing to assume that the entire set of DNA sequences have a single evolutionary origi ...
reviews - Docentes
reviews - Docentes

... with different levels of relative sequence divergence. The divergence from the most similar euryarchaeal homologue was calculated for all annotated open reading frames of a Gram-positive bacterium with little interdomain transfer (Streptococcus thermophilus LMG 18311, red) and for the extremely ther ...
You and your genes - Delivery guide
You and your genes - Delivery guide

... There are many activities that can be used as starters or plenaries (such as Activity 12) and these mini-activities can support engagement and assessment for learning. This is particularly important in a topic that can be seen as challenging, both in terms of practical and mathematical skills requir ...
A land plant-specific multigene family in the unicellular
A land plant-specific multigene family in the unicellular

... Cook, and Busse 2000 for discussion). If BIP2-like sequences are relevant to the evolution of the histogenetic meristem in the earliest land plants (and thus are of potential significance to land plant evolution), it is remarkable that such sequences are also found in both the unicellular charophyce ...
Investigation 1: Identify the Transcriptional Unit
Investigation 1: Identify the Transcriptional Unit

... lie beyond the 3' end of the coding region (i.e. thick black boxes in the FlyBase Genes track). The pre-mRNA molecule will thus carry the signal AAUAAA. This AAUAAA signal is recognized by a special endonuclease that cuts at a site 11 to 30 nucleotides to its 3' side. As you will learn in the mRNA p ...
Text S1, DOCX file, 0.03 MB
Text S1, DOCX file, 0.03 MB

... Evaluating scaffold 158 for misassembly. We considered the likelihood of scaffold misassembly to evaluate if genes from a different organism could have been merged with scaffold 158. The average coverage of the scaffold was ~41x, inclusive of all samples. An evaluation of the per-base coverage indic ...
Information Encoding in Biological Molecules: DNA and
Information Encoding in Biological Molecules: DNA and

... sequence data and examine the expression of the human orthologs. • Enter any gene to start. • In configure menu: (a) Expand tissue selection of GNF Atlas 2 to “median of replicas”, (b) click on human homologs • In filter menu: (a) set adrenal gland minimum box to 2.5, (b) look at results and set max ...
Name that Gene
Name that Gene

... This will take you to a page with lots of information about your gene and what it does. Some of the information may be difficult to follow. You can always search on the internet to find out more! ***So that is your basic tour of the NCBI. There is lots of other information about genes and DNA posted ...
Mitochondriontoplastid DNA transfer: it happens
Mitochondriontoplastid DNA transfer: it happens

... In light of these new data from carrots and milkweeds, it is safe to say that mitochondrion-to-plastid DNA transfer does occur (but the reverse process occurs much more frequently). It is still not New Phytologist (2014) 202: 736–738 www.newphytologist.com ...
module 2: transcription part i
module 2: transcription part i

... lie beyond the 3' end of the coding region (i.e. thick black boxes in the FlyBase Genes track). The pre-mRNA molecule will thus carry the signal AAUAAA. This AAUAAA signal is recognized by a special endonuclease that cuts at a site 11 to 30 nucleotides to its 3' side. As you will learn in the mRNA p ...
Gill: Transcription Regulation I
Gill: Transcription Regulation I

... Terminology • Promoter: The region of DNA 100-1,000bp immediately “upstream” of the TSS, which encodes binding sites for the general purpose RNA polymerase associated TFs, and at times some context specific sites. – There are as many promoters as there are TSS’s in the human genome. Many genes have ...
doc - Sol Genomics Network
doc - Sol Genomics Network

... other chromosome provide some services for other groups in exchange for the money they saved? DZ - Perhaps that’s there fault for sequencing the wrong chromosome! Don’t bother finishing a BAC from another chromosome if it’s already been finished by the other project. WGS discussion. So far no idea h ...
Acidaminococcus fermentans type strain (VR4T)
Acidaminococcus fermentans type strain (VR4T)

... of Sanger and 454 sequencing platforms. All general aspects of library construction and sequencing can be found at http://www.jgi.doe.gov/. 454 Pyrosequencing reads were assembled using the Newbler assembler version 2.0.0-PostRelease11/04/2008 (Roche). Large Newbler contigs were broken into 2,561 ov ...
Improving Intergenic miRNA Target Genes Prediction
Improving Intergenic miRNA Target Genes Prediction

... genes, but it doesn’t necessarily include all real target genes (because of supporting data limitation) ...
gene_prediction_20040930
gene_prediction_20040930

... 2. Perform database similarity search of expressed sequence tag Sites (EST) database of same organism, or cDNA sequences if available Use gene prediction program to locate genes ...
Why Gene Duplication? ,
Why Gene Duplication? ,

... individual Xenopus, which is homozygous for a deletion of the nucleolar organizer is totally incapable of synthesizing 185 and 28S ribosomal RNA. Yet such a homozygous deficient zygote derived from the mating of heterozygotes grows to the swimming tadpole stage (ELSDALE et al., 1958). The amount of ...
Facts about the mini-Tn7 transposon system as a tool for
Facts about the mini-Tn7 transposon system as a tool for

... sequences located on the delivery plasmid between the transposon ends, Tn7L and Tn7R (see Fig. 2). The delivery plasmid is some times also named the carrier plasmid. Delivery plasmids The delivery plasmids presented here are all pUC19 derivatives, which can replicate in E. coli and other Enterics, b ...
Gene targeting in filamentous fungi: the benefits of impaired repair
Gene targeting in filamentous fungi: the benefits of impaired repair

... integration in NHEJ-deficient genetic backgrounds were published, such as for the human pathogens Aspergillus fumigatus or Cryptococcus neoformans, the model organism Aspergillus nidulans, the koji molds Aspergillus oryzae and Aspergillus sojae, and the filamentous ascomycete Sordaria macrospora (Ta ...
Molecular phylogeny, part B
Molecular phylogeny, part B

... Molecular phylogenetics: A set of techniques that enable the evolutionary relationships between DNA sequences to be inferred by making comparisons between those sequences. Multigene family: A group of genes, clustered or dispersed, with related nucleotide sequences. Multiple alignment: An alignment ...
REVIEWS - Ken Wolfe`s
REVIEWS - Ken Wolfe`s

... The renewed interest in Ohno’s ideas stems from two lines of research that began to bear fruit in the late 1980s. The first was what is now called comparative genomics. Genetic map comparisons among mammals confirmed that they contain large segments4 of conserved SYNTENY with conserved gene order. A ...
Characterization of an IS-like element from
Characterization of an IS-like element from

... sequence is present, which is nearly identical to that of the 65 kDa heat shock protein of M . tuberculosis (data not shown), but no obvious transcriptional signals can be found. The further analysis of the nucleotide sequence revealed some interesting features such as long inverted repeats in front ...
Reductive evolution of resident genomes
Reductive evolution of resident genomes

... 1-Mbp range19. The free-living bacteria from which these parasites have evolved probably had genomes that were 4–5 times larger13,19,32. Such genomic shrinkage is not a random process, in the sense that any and all genes may be deleted from the genome: it is potentially less damaging to a parasite t ...
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Transposable element



A transposable element (TE or transposon) is a DNA sequence that can change its position within the genome, sometimes creating or reversing mutations and altering the cell's genome size. Transposition often results in duplication of the TE. Barbara McClintock's discovery of these jumping genes earned her a Nobel prize in 1983.TEs make up a large fraction of the C-value of eukaryotic cells. There are at least two classes of TEs: class I TEs generally function via reverse transcription, while class II TEs encode the protein transposase, which they require for insertion and excision, and some of these TEs also encode other proteins. It has been shown that TEs are important in genome function and evolution. In Oxytricha, which has a unique genetic system, they play a critical role in development. They are also very useful to researchers as a means to alter DNA inside a living organism.
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