Sequencing and analysis of the nucleocapsid (N) and polymerase (L)
... Full-length N clones were isolated from the first library by screening with D-74, a previously identified N-gene specific eDNA (Diallo et al., 1989). L gene-specific clones were isolated from the genomic library by screening with the 4962 bp XmnI-XmnI fragment of the measles L gene isolated from pla ...
... Full-length N clones were isolated from the first library by screening with D-74, a previously identified N-gene specific eDNA (Diallo et al., 1989). L gene-specific clones were isolated from the genomic library by screening with the 4962 bp XmnI-XmnI fragment of the measles L gene isolated from pla ...
Document
... These two Aspergillus genes encode identical proteins! This appears to be more prevalent in some taxa than others; intron gain and loss in the mammals appears to be rare. Nevertheless, in other taxa it is more common, and must be addressed by gene prediction programs. ...
... These two Aspergillus genes encode identical proteins! This appears to be more prevalent in some taxa than others; intron gain and loss in the mammals appears to be rare. Nevertheless, in other taxa it is more common, and must be addressed by gene prediction programs. ...
File
... appeared to be “linked” together in ways that, at first glance, seemed to violate the principle of independent assortment. For example, a fly with reddish-orange eyes and miniature wings, like the one shown in Figure 11–18, was used in a series of crosses. The results showed that the genes for those ...
... appeared to be “linked” together in ways that, at first glance, seemed to violate the principle of independent assortment. For example, a fly with reddish-orange eyes and miniature wings, like the one shown in Figure 11–18, was used in a series of crosses. The results showed that the genes for those ...
Globin gene family
... introns and gene-related regulatory sequences • Intergenic DNA is noncoding DNA found between genes – Pseudogenes are former genes that have accumulated mutations and are nonfunctional – Repetitive DNA is present in multiple copies in the genome ...
... introns and gene-related regulatory sequences • Intergenic DNA is noncoding DNA found between genes – Pseudogenes are former genes that have accumulated mutations and are nonfunctional – Repetitive DNA is present in multiple copies in the genome ...
An Introduction to Genetic Analysis Chapter 14 Genomics Chapter
... design principles of living organisms and for the discovery of new genes such as those responsible for human genetic disease. ...
... design principles of living organisms and for the discovery of new genes such as those responsible for human genetic disease. ...
Read the first chapter
... Delhi in 1975, when I was five years old. His mind was also crumbling. Tall and rail thin, with a slightly feral look in his eyes and a shock of matted, overgrown hair, he resembled a Bengali Jim Morrison. Unlike Rajesh, whose illness had surfaced in his twenties, Jagu had been troubled from childho ...
... Delhi in 1975, when I was five years old. His mind was also crumbling. Tall and rail thin, with a slightly feral look in his eyes and a shock of matted, overgrown hair, he resembled a Bengali Jim Morrison. Unlike Rajesh, whose illness had surfaced in his twenties, Jagu had been troubled from childho ...
Supplementary Material Legends
... border genomic DNA-T-DNA fusion site was known (Suppl. Info. 1). In these cases, it was assumed that the T-DNA insertion had happened without DNA sequence deletion and sequence feature analysis for the “unknown” side was started at the nucleotide directly adjacent to the known genomic DNA-T-DNA fusi ...
... border genomic DNA-T-DNA fusion site was known (Suppl. Info. 1). In these cases, it was assumed that the T-DNA insertion had happened without DNA sequence deletion and sequence feature analysis for the “unknown” side was started at the nucleotide directly adjacent to the known genomic DNA-T-DNA fusi ...
Analysis of a piwi-related Gene Implicates Small RNAs in
... 1.The next slide describes the enzymatic machineries that are likely to be involved in IES elimination by the scnRNA mechanism. 2. Evidence demonstrating the nature of the RNA polymerase and the RNAse III (dicer) were presented at the meeting. However, these data are unpublished and making this pre ...
... 1.The next slide describes the enzymatic machineries that are likely to be involved in IES elimination by the scnRNA mechanism. 2. Evidence demonstrating the nature of the RNA polymerase and the RNAse III (dicer) were presented at the meeting. However, these data are unpublished and making this pre ...
Significance analysis of microarrays (SAM)
... For each permutation of the data, compute the number of positive and negative significant genes for a given delta as explained in the previous slide. The median number of significant genes from these permutations is the median False Discovery Rate. The rationale behind this is, any genes designated ...
... For each permutation of the data, compute the number of positive and negative significant genes for a given delta as explained in the previous slide. The median number of significant genes from these permutations is the median False Discovery Rate. The rationale behind this is, any genes designated ...
Understanding the Adaptation of Halobacterium Species NRC
... biosynthesis, were also localized to a second minor peak (Fig. 4, peak 2). Interestingly, gene order for the nuo genes is conserved with respect to E. coli, and for the men genes is conserved compared with both E. coli and Deinococcus radiodurans. GC analysis of these two groups of genes showed them ...
... biosynthesis, were also localized to a second minor peak (Fig. 4, peak 2). Interestingly, gene order for the nuo genes is conserved with respect to E. coli, and for the men genes is conserved compared with both E. coli and Deinococcus radiodurans. GC analysis of these two groups of genes showed them ...
Foundations of Biology
... In addition to promoters, enhancers also influence the expression of genes Control of gene expression in eukaryotes involves many more factors than control in prokaryotes This allows much finer control of gene expression ©2000 Timothy G. Standish ...
... In addition to promoters, enhancers also influence the expression of genes Control of gene expression in eukaryotes involves many more factors than control in prokaryotes This allows much finer control of gene expression ©2000 Timothy G. Standish ...
- Environmental Biosafety Research
... Introduction of the oligonucleotides in the cells can be performed without using any delivery vector system via different techniques such as electroporation, lipofection, transfection or particle bombardment (biolistic) (see e.g. Radecke et al., 2006). OMM does not involve the introduction or integra ...
... Introduction of the oligonucleotides in the cells can be performed without using any delivery vector system via different techniques such as electroporation, lipofection, transfection or particle bombardment (biolistic) (see e.g. Radecke et al., 2006). OMM does not involve the introduction or integra ...
Next generation sequencing
... oligonucleotides) correspond to each fluorophore. If first or second nucleotide (in dinucleotide) is known, colour is unambiguously related with the other nucleotide. Three next positions — degenerate nucleotides: 64 different versions for each particular dinucleotide. When ligated to the sequencing ...
... oligonucleotides) correspond to each fluorophore. If first or second nucleotide (in dinucleotide) is known, colour is unambiguously related with the other nucleotide. Three next positions — degenerate nucleotides: 64 different versions for each particular dinucleotide. When ligated to the sequencing ...
Features on Nucleic Acid Sequences, Gene Features and Coding
... to sequences via locations requires the use of more than one table. Simple examples include a promoter, or a repeat region, or a UTR on an NA Sequence. In each case, there is a sequence, there is a feature located on that sequence, and there is a span on that sequence where the feature is located. E ...
... to sequences via locations requires the use of more than one table. Simple examples include a promoter, or a repeat region, or a UTR on an NA Sequence. In each case, there is a sequence, there is a feature located on that sequence, and there is a span on that sequence where the feature is located. E ...
ucsc genome research primer - Center for Biomolecular Science
... Bioinformatics Group works to make the human genome sequence even more useful for science and medicine by identifying and annotating its key functional elements in such a way that they are easily accessible to researchers. This process of discovery and categorization is a critical step toward fully ...
... Bioinformatics Group works to make the human genome sequence even more useful for science and medicine by identifying and annotating its key functional elements in such a way that they are easily accessible to researchers. This process of discovery and categorization is a critical step toward fully ...
Regulation of Transcription
... In addition to promoters, enhancers also influence the expression of genes Control of gene expression in eukaryotes involves many more factors than control in prokaryotes This allows much finer control of gene expression ©2000 Timothy G. Standish ...
... In addition to promoters, enhancers also influence the expression of genes Control of gene expression in eukaryotes involves many more factors than control in prokaryotes This allows much finer control of gene expression ©2000 Timothy G. Standish ...
Scenario: Phage Wars Identification of a Bacteriophage 80α
... with that of the bacterial chromosome. This so-called prophage state is maintained by a phageencoded immunity repressor that prevents expression of the phage lytic genes. Bacterial cells carrying prophages are called lysogens. The immunity repressor binds to specific DNA sequences, called operators, ...
... with that of the bacterial chromosome. This so-called prophage state is maintained by a phageencoded immunity repressor that prevents expression of the phage lytic genes. Bacterial cells carrying prophages are called lysogens. The immunity repressor binds to specific DNA sequences, called operators, ...
Greedy Algorithms - University of Illinois at Urbana
... • ImprovedBreakPointReversalSort is an approximation algorithm with a performance guarantee of at most 4 – It eliminates at least one breakpoint in every two steps; at most 2b(p) steps – Approximation ratio: 2b(p) / d(p) – Optimal algorithm eliminates at most 2 breakpoints in every step: d(p) b(p) ...
... • ImprovedBreakPointReversalSort is an approximation algorithm with a performance guarantee of at most 4 – It eliminates at least one breakpoint in every two steps; at most 2b(p) steps – Approximation ratio: 2b(p) / d(p) – Optimal algorithm eliminates at most 2 breakpoints in every step: d(p) b(p) ...
EXTENSION OF MENDELIAN INHERITANCE: BEYOND
... genes that occur outside the nuclear chromosomes i.e. from mitochondrial DNA or chloroplast DNA. It is found in most eukaryotes and is commonly known to occur in cytoplasmic organelles such as mitochondria and chloroplasts. Extranuclear Inheritance of Organelles Mitochondria are organelles which fun ...
... genes that occur outside the nuclear chromosomes i.e. from mitochondrial DNA or chloroplast DNA. It is found in most eukaryotes and is commonly known to occur in cytoplasmic organelles such as mitochondria and chloroplasts. Extranuclear Inheritance of Organelles Mitochondria are organelles which fun ...
Dimerization of GT element interferes negatively with gene activation
... region. The spacing between two GT elements seems to be critical for their activity. An increase in spacing between two GT elements even by 2 bp can dramatically reduce the transcript level (Gilmartin and Chua 1990), as in the case of rbcS-3A, suggesting critical sequence requirements for the activa ...
... region. The spacing between two GT elements seems to be critical for their activity. An increase in spacing between two GT elements even by 2 bp can dramatically reduce the transcript level (Gilmartin and Chua 1990), as in the case of rbcS-3A, suggesting critical sequence requirements for the activa ...
Genome Analysis Excerpt from Chapter 11
... There are several classes of sequences (transposable elements) that can move from one genome location to another, thus affecting gene content. Highly repetitive sequences in the genome are derived from such classes of sequences that move (transpose) from one genome location to another. These sequenc ...
... There are several classes of sequences (transposable elements) that can move from one genome location to another, thus affecting gene content. Highly repetitive sequences in the genome are derived from such classes of sequences that move (transpose) from one genome location to another. These sequenc ...
pEGFP-C1 - Newcastle University Staff Publishing Service
... Fusions to the C terminus of EGFP retain the fluorescent properties of the native protein allowing the localization of the fusion protein in vivo. The target gene should be cloned into pEGFP-C1 so that it is in frame with the EGFP coding sequences, with no intervening in-frame stop codons. The recom ...
... Fusions to the C terminus of EGFP retain the fluorescent properties of the native protein allowing the localization of the fusion protein in vivo. The target gene should be cloned into pEGFP-C1 so that it is in frame with the EGFP coding sequences, with no intervening in-frame stop codons. The recom ...
The Title of the Article
... which results from a recently published Type 2 Diabetes association study are used to identify potential hypotheses. Introduction Identifying, confirming, and validating candidate genes in complex diseases is a daunting task. A genome-wide association study, in particular, can generate an extremely ...
... which results from a recently published Type 2 Diabetes association study are used to identify potential hypotheses. Introduction Identifying, confirming, and validating candidate genes in complex diseases is a daunting task. A genome-wide association study, in particular, can generate an extremely ...
Transcription - Faculty Web Pages
... • Must all genes encode a protein? • What are the different classes of RNA and their functions? • If an mRNA is translated in the 5’ 3’ direction, which DNA strand is therefore the coding or template strand for the gene? If RNA were made off of the other DNA strand, would it code for protein? • In ...
... • Must all genes encode a protein? • What are the different classes of RNA and their functions? • If an mRNA is translated in the 5’ 3’ direction, which DNA strand is therefore the coding or template strand for the gene? If RNA were made off of the other DNA strand, would it code for protein? • In ...
file
... •3,000 most variable ORFs were chosen (based on the normalized dispersion in expression level of each gene across the time points (s.d./mean). •The 15 time points were used to construct a 3,000 by 15 data matrix. •The variance of each gene was normalized across the 15 conditions: ...
... •3,000 most variable ORFs were chosen (based on the normalized dispersion in expression level of each gene across the time points (s.d./mean). •The 15 time points were used to construct a 3,000 by 15 data matrix. •The variance of each gene was normalized across the 15 conditions: ...
Transposable element
A transposable element (TE or transposon) is a DNA sequence that can change its position within the genome, sometimes creating or reversing mutations and altering the cell's genome size. Transposition often results in duplication of the TE. Barbara McClintock's discovery of these jumping genes earned her a Nobel prize in 1983.TEs make up a large fraction of the C-value of eukaryotic cells. There are at least two classes of TEs: class I TEs generally function via reverse transcription, while class II TEs encode the protein transposase, which they require for insertion and excision, and some of these TEs also encode other proteins. It has been shown that TEs are important in genome function and evolution. In Oxytricha, which has a unique genetic system, they play a critical role in development. They are also very useful to researchers as a means to alter DNA inside a living organism.