Mobile genetic elements in antibiotic resistance
... today. This was, however, a very ancient event. Resistance plasmids are similar to plasmids found in strains of gram-negative rods isolated before the antibiotic era that do not carry resistance mechanisms, indicating that resistance genes must have been acquired since the introduction of antibiotic ...
... today. This was, however, a very ancient event. Resistance plasmids are similar to plasmids found in strains of gram-negative rods isolated before the antibiotic era that do not carry resistance mechanisms, indicating that resistance genes must have been acquired since the introduction of antibiotic ...
BrownCNA Thank you with the QC checking of this genome. It was
... Thank you with the QC checking of this genome. It was pretty straightforward and we had 2 different class sections work on the annotations that were compared for the final file. We had 2 genes that I would like help another opinion on. Larry’s class added one ORF, but it was not added by mine; both ...
... Thank you with the QC checking of this genome. It was pretty straightforward and we had 2 different class sections work on the annotations that were compared for the final file. We had 2 genes that I would like help another opinion on. Larry’s class added one ORF, but it was not added by mine; both ...
Evolution
... Project were able to identify and map the 20,000–25,000 genes that define a human being. The project also successfully mapped the genomes of other species, including the fruit fly, mouse, and Escherichia coli. The location and complete sequence of the genes in each of these species are available for ...
... Project were able to identify and map the 20,000–25,000 genes that define a human being. The project also successfully mapped the genomes of other species, including the fruit fly, mouse, and Escherichia coli. The location and complete sequence of the genes in each of these species are available for ...
Slides
... • Service, R. F. (2001). The human genome: Objection #3: impossible to do. Science, 291(5507), 1186. – Technology of the time permitted 500 bp per day per person. – Move from radioactively labeled sequencing to fuorescent sequencing permitted complete automation up to 1 gigabyte per year. ...
... • Service, R. F. (2001). The human genome: Objection #3: impossible to do. Science, 291(5507), 1186. – Technology of the time permitted 500 bp per day per person. – Move from radioactively labeled sequencing to fuorescent sequencing permitted complete automation up to 1 gigabyte per year. ...
Presentation - Cloudfront.net
... Clusters of Orthologous Groups of proteins (COGs) were delineated by comparing protein sequences encoded in complete genomes, representing major phylogenetic lineages. Each COG consists of individual proteins or groups of paralogs from at least 3 lineages and thus corresponds to an ancient conserve ...
... Clusters of Orthologous Groups of proteins (COGs) were delineated by comparing protein sequences encoded in complete genomes, representing major phylogenetic lineages. Each COG consists of individual proteins or groups of paralogs from at least 3 lineages and thus corresponds to an ancient conserve ...
Genome Research 13, 8 - Tel
... to uniform. Similar findings were obtained for other organisms. Several measures based on the phase property are proposed. The measures are computed by clockwise rotation of the vectors, obtained by DFT for each analysis frame, by an angle equal to the corresponding central value. In protein coding ...
... to uniform. Similar findings were obtained for other organisms. Several measures based on the phase property are proposed. The measures are computed by clockwise rotation of the vectors, obtained by DFT for each analysis frame, by an angle equal to the corresponding central value. In protein coding ...
Positive Control and Catabolite Repression
... • Structural genes: encoding proteins • Regulatory genes: encoding products that interact with other sequences and affect the transcription and translation of these sequences • Regulatory elements: DNA sequences that are not transcribed but play a role in regulating other nucleotide sequences ...
... • Structural genes: encoding proteins • Regulatory genes: encoding products that interact with other sequences and affect the transcription and translation of these sequences • Regulatory elements: DNA sequences that are not transcribed but play a role in regulating other nucleotide sequences ...
Control, Genomes and Environment
... cell. This cell divides and the new cells produced start to differentiate and specialize. ‘Switching on’ the expression of a gene or keeping it switched off determines the development of features. ...
... cell. This cell divides and the new cells produced start to differentiate and specialize. ‘Switching on’ the expression of a gene or keeping it switched off determines the development of features. ...
A1993LB48800002
... than 200, distinct cytochrome P450 (CYP) genes, almost all of which encode a unique enzyme.4 Why should there be so many CYP genes? One likely explanation—at least in animals during the last 400 million years—is the evolving of new genes by molecularly driven duplication events, in response to dieta ...
... than 200, distinct cytochrome P450 (CYP) genes, almost all of which encode a unique enzyme.4 Why should there be so many CYP genes? One likely explanation—at least in animals during the last 400 million years—is the evolving of new genes by molecularly driven duplication events, in response to dieta ...
The Question of Questions: What is a Gene? Comments on Rolston
... For present purposes, I want to pick out from Aristotle two important insights he got right. Aristotle recognized that living beings, organisms, must be understood in all respects as ends-untothemselves. What they do, how they are, how they develop and change can only be understood as being neither ...
... For present purposes, I want to pick out from Aristotle two important insights he got right. Aristotle recognized that living beings, organisms, must be understood in all respects as ends-untothemselves. What they do, how they are, how they develop and change can only be understood as being neither ...
Patterns of gene duplication and sex chromosomes evolution
... – I also like to speculate that they might also have an interplay with sexual antagonism Supported by loss of new retrogenes, loss of functions of the new retrogenes, and lack of infertility effects of null alleles of Dntf-2r (Tracy et al. In preparation) and high turnover of species restricted ...
... – I also like to speculate that they might also have an interplay with sexual antagonism Supported by loss of new retrogenes, loss of functions of the new retrogenes, and lack of infertility effects of null alleles of Dntf-2r (Tracy et al. In preparation) and high turnover of species restricted ...
Exercise - GEP Community Server - Washington University in St. Louis
... chromosome. Clicking on each exon in the exon table, reveals the sequence that corresponds to the selected exon. In order to generate a gene model, we will use both polypeptide and transcript details. In this simple example, there is only one transcript and one protein isoform and all exons are codi ...
... chromosome. Clicking on each exon in the exon table, reveals the sequence that corresponds to the selected exon. In order to generate a gene model, we will use both polypeptide and transcript details. In this simple example, there is only one transcript and one protein isoform and all exons are codi ...
SEGMENTAL VARIATION
... • Depth of coverage in a single patient was compared to average and standard deviation of depth of coverage. • Algorithms were developed for: – Classifying X chromosome as being deleted in males compared with females – Classifying X chromosome as being duplicated in females compared with males S L I ...
... • Depth of coverage in a single patient was compared to average and standard deviation of depth of coverage. • Algorithms were developed for: – Classifying X chromosome as being deleted in males compared with females – Classifying X chromosome as being duplicated in females compared with males S L I ...
Horizontal gene transfer and the origin of species: lessons from
... slower pace than that of plasmids, and would obviously create new bacterial subspecies (i.e. the recombinants have discernibly different phenotypes). Because multiple pathogenicity islands, varying in size and in location on the host chromosome, can be found in any bacterial strain, they must have b ...
... slower pace than that of plasmids, and would obviously create new bacterial subspecies (i.e. the recombinants have discernibly different phenotypes). Because multiple pathogenicity islands, varying in size and in location on the host chromosome, can be found in any bacterial strain, they must have b ...
MAK, a computational tool kit for automated MITE
... identified in higher eukaryotes: type I elements (retrotransposable elements) use a copy–paste approach to transpose, yielding a large copy number; type II elements (DNA elements) use a cut–paste–repair approach to transpose. However, numerous families of highly repetitive (hundreds or thousands), s ...
... identified in higher eukaryotes: type I elements (retrotransposable elements) use a copy–paste approach to transpose, yielding a large copy number; type II elements (DNA elements) use a cut–paste–repair approach to transpose. However, numerous families of highly repetitive (hundreds or thousands), s ...
A novel human cytochrome P4S0 gene (P450IIB): chromosomal
... We have isolated from a single human liver cDNA library two clones which are highly homologous (78% over the coding region) to the major phenobarbital-inducible P450 from rat (P450IIB1) . This is the first direct demonstration of the presence of the P450IIB gene subfamily in humans. This subfamily i ...
... We have isolated from a single human liver cDNA library two clones which are highly homologous (78% over the coding region) to the major phenobarbital-inducible P450 from rat (P450IIB1) . This is the first direct demonstration of the presence of the P450IIB gene subfamily in humans. This subfamily i ...
Presentazione di PowerPoint
... To reconstruct the history of genes families, under the hypothesis that every family member derives from a duplication process of another member, means to put the set of members into a tree, that we call paralogy tree, in which the root represents the most ancient gene of the family, and each direct ...
... To reconstruct the history of genes families, under the hypothesis that every family member derives from a duplication process of another member, means to put the set of members into a tree, that we call paralogy tree, in which the root represents the most ancient gene of the family, and each direct ...
Sleeping beauty: a novel cancer gene discovery tool
... the first cut-and-paste transposon shown to have activity in mice (22). SB is a member of the Tc1/mariner family of transposable elements reconstructed from ancient elements found in the genomes of salmon fish. The system consists of two parts: the transposase enzyme and the DNA transposon vector. I ...
... the first cut-and-paste transposon shown to have activity in mice (22). SB is a member of the Tc1/mariner family of transposable elements reconstructed from ancient elements found in the genomes of salmon fish. The system consists of two parts: the transposase enzyme and the DNA transposon vector. I ...
Location Analysis of Transcription Factor Binding - CS
... 1,239 novel genes (RNA genes?) Clusters of active promoters (chromatin) Four classes of promoters ...
... 1,239 novel genes (RNA genes?) Clusters of active promoters (chromatin) Four classes of promoters ...
Supplementary Information (doc 83K)
... The reporter plasmid pBIO1878 was made by cloning a 2 kb BamHI SpcR cassette fragment from plasmid pHP45 (Prentki and Krisch, 1984) into the BglII site of pMP220, which is a wide hostrange promoter-probe plasmid with a lacZ gene lacking its native promoter (Spaink et al., 1987). The region of the R ...
... The reporter plasmid pBIO1878 was made by cloning a 2 kb BamHI SpcR cassette fragment from plasmid pHP45 (Prentki and Krisch, 1984) into the BglII site of pMP220, which is a wide hostrange promoter-probe plasmid with a lacZ gene lacking its native promoter (Spaink et al., 1987). The region of the R ...
A THREE-GENERATION APPROACH IN BIODEMOGRAPHY IS
... This will likely lead to novel and more powerful data collection designs for the study of innate and acquired genetic mutations in both healthy and diseased organisms. For example, in the Mayak production facility catchment area, data was collected on three generations (30,000 offspring) during 53 y ...
... This will likely lead to novel and more powerful data collection designs for the study of innate and acquired genetic mutations in both healthy and diseased organisms. For example, in the Mayak production facility catchment area, data was collected on three generations (30,000 offspring) during 53 y ...
Identification of porcine Lhx3 and SF1 as candidate genes for QTL
... intron 2 (accession number AF345446). For sequencing of the MARC reference mapping parents, a pair of nested primers (MARC6871/6872) were used to obtain higher quality ampli®cation for direct sequencing of PCR products. The nucleotide sequence of the amplicon produced, with polymorphic positions ind ...
... intron 2 (accession number AF345446). For sequencing of the MARC reference mapping parents, a pair of nested primers (MARC6871/6872) were used to obtain higher quality ampli®cation for direct sequencing of PCR products. The nucleotide sequence of the amplicon produced, with polymorphic positions ind ...
GENE 760 -â Problem Set #3
... but this does not necessarily mean that both reads are high quality and/or mappable. Due to this inconsistency, RPKM will end up counting both reads for some fragments and only one for others, creati ...
... but this does not necessarily mean that both reads are high quality and/or mappable. Due to this inconsistency, RPKM will end up counting both reads for some fragments and only one for others, creati ...
Answers to test 2
... Based upon these maps, which of the following is correct? a) pathogen resistance is most likely caused by genes G6 and/or G7 b) pathogen resistance is most likely caused by genes G5 and/or G6 and/or G7 c) pathogen resistance is caused by any one of the genes shown on the physical map d) none of the ...
... Based upon these maps, which of the following is correct? a) pathogen resistance is most likely caused by genes G6 and/or G7 b) pathogen resistance is most likely caused by genes G5 and/or G6 and/or G7 c) pathogen resistance is caused by any one of the genes shown on the physical map d) none of the ...
Transposable element
A transposable element (TE or transposon) is a DNA sequence that can change its position within the genome, sometimes creating or reversing mutations and altering the cell's genome size. Transposition often results in duplication of the TE. Barbara McClintock's discovery of these jumping genes earned her a Nobel prize in 1983.TEs make up a large fraction of the C-value of eukaryotic cells. There are at least two classes of TEs: class I TEs generally function via reverse transcription, while class II TEs encode the protein transposase, which they require for insertion and excision, and some of these TEs also encode other proteins. It has been shown that TEs are important in genome function and evolution. In Oxytricha, which has a unique genetic system, they play a critical role in development. They are also very useful to researchers as a means to alter DNA inside a living organism.