file - ORCA - Cardiff University
... Generating molecular diversity lies at the heart of directed evolution (1-4). With an everexpanding directed evolution toolbox, different mutational approaches are required. These will access sequence space beyond that sampled by simple gene point mutations that ultimately restrict sequence, structu ...
... Generating molecular diversity lies at the heart of directed evolution (1-4). With an everexpanding directed evolution toolbox, different mutational approaches are required. These will access sequence space beyond that sampled by simple gene point mutations that ultimately restrict sequence, structu ...
Lecture 8
... * These paralogs are more similar to each other than to orthologs → result of duplication after the species split * The remaining 13% (=253 ORFs) perhaps older paralogs that have been lost in the other species due to specialisation ...
... * These paralogs are more similar to each other than to orthologs → result of duplication after the species split * The remaining 13% (=253 ORFs) perhaps older paralogs that have been lost in the other species due to specialisation ...
Genome Sequence Quality - Rice Genome Annotation Project
... WGS submissions may also have annotation associated with them, but the unannotated sequence files can have a separate project ID from the annotated files (just keep in mind) The rice indica sequence assembly is maintained in the WGS database at NCBI (AAAAxxxxxxxx) ...
... WGS submissions may also have annotation associated with them, but the unannotated sequence files can have a separate project ID from the annotated files (just keep in mind) The rice indica sequence assembly is maintained in the WGS database at NCBI (AAAAxxxxxxxx) ...
Whole-Genome Sequence and Variant Analysis of W303, a Widely
... rearrangement discussed above. A comparison of the gene content between S288C and the annotation of W303 shows expansion of Y' element ATPdependent helicase protein throughout the genome including the acquisition of Y’ regions on chromosomes without these subtelomeric elements in S288C. These differ ...
... rearrangement discussed above. A comparison of the gene content between S288C and the annotation of W303 shows expansion of Y' element ATPdependent helicase protein throughout the genome including the acquisition of Y’ regions on chromosomes without these subtelomeric elements in S288C. These differ ...
From essential to persistent genes: a functional
... a pathogen. These studies were carried out on libraries of microorganisms using transposons or antisense RNA expression [4], but the ‘minimal set’ outcomes differed widely in terms of gene number (and often identity), not only in distant organisms but also in the same organism under different (and s ...
... a pathogen. These studies were carried out on libraries of microorganisms using transposons or antisense RNA expression [4], but the ‘minimal set’ outcomes differed widely in terms of gene number (and often identity), not only in distant organisms but also in the same organism under different (and s ...
Evolution of Gene Expression
... extensively analyzed, allowing predictions to be made about the consequences of some observed changes. These types of analyses provide insight not only into evolutionary processes, but also into enhancer architecture in general. If enhancer sequences can change extensively and still retain their ori ...
... extensively analyzed, allowing predictions to be made about the consequences of some observed changes. These types of analyses provide insight not only into evolutionary processes, but also into enhancer architecture in general. If enhancer sequences can change extensively and still retain their ori ...
Full-Text PDF
... number of hits (22) was found on chromosome 10, with chromosome 5 harboring six LTRs. Four LTRs, on chromosomes 5 (2×), 7 and 20, were followed by a PBS(Lys3) sequence. 3.4. The LTR of the Horse Endogenous Retrovirus EqERV-Beta1 LTR sequences of the provirus started with the sequence TG and ended wi ...
... number of hits (22) was found on chromosome 10, with chromosome 5 harboring six LTRs. Four LTRs, on chromosomes 5 (2×), 7 and 20, were followed by a PBS(Lys3) sequence. 3.4. The LTR of the Horse Endogenous Retrovirus EqERV-Beta1 LTR sequences of the provirus started with the sequence TG and ended wi ...
genetic control of the malaria mosquito using gene
... gene drive can be used to suppress mosquito populations or to render them unable to transmit disease proof of principle implementations for both approaches have been demonstrated not a silver bullet, must work alongside other interventions that are already having an impact (e.g. bednets, drugs) work ...
... gene drive can be used to suppress mosquito populations or to render them unable to transmit disease proof of principle implementations for both approaches have been demonstrated not a silver bullet, must work alongside other interventions that are already having an impact (e.g. bednets, drugs) work ...
GENESIS: genome evolution scenarios
... the most common rearrangements are inversions (also called reversals in bioinformatics), where—from a mathematical point of view—a section of the genome is excised, reversed in orientation and re-inserted. Biologically, inversions can be caused by replication errors. But also large-scale duplication ...
... the most common rearrangements are inversions (also called reversals in bioinformatics), where—from a mathematical point of view—a section of the genome is excised, reversed in orientation and re-inserted. Biologically, inversions can be caused by replication errors. But also large-scale duplication ...
Heartwood extractives – from phenotype to candidate genes
... According to earlier studies, the pinosylvin synthase gene is present in five copies in the Scots pine genome (PST-1 through PST-5; Preisig-Müller et al. 1999). All gene family members have two exons and a single intron in a conserved site. PST-1 was identified as the most active gene, which accordi ...
... According to earlier studies, the pinosylvin synthase gene is present in five copies in the Scots pine genome (PST-1 through PST-5; Preisig-Müller et al. 1999). All gene family members have two exons and a single intron in a conserved site. PST-1 was identified as the most active gene, which accordi ...
Genetic regulation in eukaryotes
... identified in the nucleolus, where they guide nucleotide modification in rRNA at specific positions. They do this by forming short duplexes with a sequence of the rRNA that contains the target nucleotide. At least 340 human snoRNA genes have been found so far, but there may be many more because snoR ...
... identified in the nucleolus, where they guide nucleotide modification in rRNA at specific positions. They do this by forming short duplexes with a sequence of the rRNA that contains the target nucleotide. At least 340 human snoRNA genes have been found so far, but there may be many more because snoR ...
Characterization of the IEll0 Gene of Herpes Simplex Virus Type 1
... determined the complete sequence of RL, together with adjacent regions of UL: the whole sequence will be presented elsewhere (L. J. Perry & D. J. McGeoch, unpublished). Residue numbering in this paper is based on the D N A sequence of RL, starting with the residue in IRL adjacent to the a' sequence ...
... determined the complete sequence of RL, together with adjacent regions of UL: the whole sequence will be presented elsewhere (L. J. Perry & D. J. McGeoch, unpublished). Residue numbering in this paper is based on the D N A sequence of RL, starting with the residue in IRL adjacent to the a' sequence ...
GmDREB5 Tiendai, Vietnam Chu Hoang Lan Nguyen Vu Thanh Thanh
... a Chinese soybean cultivar. Amino acid sequence of the polypeptide encoded by these two genes are 87.8% similar. There are, however, differences appeared in some positions of nucleotide and amino acid sequences between this two genes and two polypeptides. This study is the basis for later studies on ...
... a Chinese soybean cultivar. Amino acid sequence of the polypeptide encoded by these two genes are 87.8% similar. There are, however, differences appeared in some positions of nucleotide and amino acid sequences between this two genes and two polypeptides. This study is the basis for later studies on ...
Genome - people.iup.edu
... Orthologs: genes found in one organism that are similar to those in another organism but differ because of speciation Orthologs are genes in different species that evolved from a common ancestral gene by speciation. Normally, orthologs retain the same function in the course of evolution. Identifica ...
... Orthologs: genes found in one organism that are similar to those in another organism but differ because of speciation Orthologs are genes in different species that evolved from a common ancestral gene by speciation. Normally, orthologs retain the same function in the course of evolution. Identifica ...
Author`s personal copy
... also necessary, at least transiently. Thus the long-term evolutionary success seems to require an intermediary level of variability, which could put an indirect selective pressure on the general features of the genotype–phenotype map (Wagner and Altenberg, 1996). The genotype–phenotype map is not, h ...
... also necessary, at least transiently. Thus the long-term evolutionary success seems to require an intermediary level of variability, which could put an indirect selective pressure on the general features of the genotype–phenotype map (Wagner and Altenberg, 1996). The genotype–phenotype map is not, h ...
![P[acman]: A BAC Transgenic Platform for Targeted Insertion of](http://s1.studyres.com/store/data/015771109_1-230af42a78d308ddb0e06d89019d2ef2-300x300.png)
P[acman]: A BAC Transgenic Platform for Targeted Insertion of
... copy number, facilitating plasmid maintenance and recombineering, but is induced to high copy number for plasmid isolation. Recombineering allows gap repair and mutagenesis in bacteria. Gap repair efficiently retrieves DNA fragments up to 133 kilobases long from P1 or BAC clones. fC31mediated transg ...
... copy number, facilitating plasmid maintenance and recombineering, but is induced to high copy number for plasmid isolation. Recombineering allows gap repair and mutagenesis in bacteria. Gap repair efficiently retrieves DNA fragments up to 133 kilobases long from P1 or BAC clones. fC31mediated transg ...
On the energy and material cost of gene duplication
... ribosomes, rendering them unavailable for expressing other genes at appropriate levels. Experimental approaches like these are powerful, because they can directly demonstrate the effects of gene expression on cell growth. However, they can detect the expression costs of only the most highly expresse ...
... ribosomes, rendering them unavailable for expressing other genes at appropriate levels. Experimental approaches like these are powerful, because they can directly demonstrate the effects of gene expression on cell growth. However, they can detect the expression costs of only the most highly expresse ...
Applied and Environmental Microbiology
... expressed throughout root nodule development and during nitrogen-fixation in the Rhizobium-Medicago symbiosis. Plant J. 9:31–43. 2. Collins, C. H., and P. M. Lyne. 1985. Microbiological methods, 5th ed. ...
... expressed throughout root nodule development and during nitrogen-fixation in the Rhizobium-Medicago symbiosis. Plant J. 9:31–43. 2. Collins, C. H., and P. M. Lyne. 1985. Microbiological methods, 5th ed. ...
iCLIP HeLa cells were UV crosslinked before lysing in lysis buffer
... (Epicentre Biotechnologies, CL9025K) at 60C for 1 hour. A primer that is complimentary to the BamHI sites was then annealed to the circular cDNA (5’GTTCAGGATCCACGACGCTCTTCAAAA-3’) and the cDNA was re-linearised by digesting with BamHI. The resulting cDNA had 3’ adaptor and 5’ adaptor at the either ...
... (Epicentre Biotechnologies, CL9025K) at 60C for 1 hour. A primer that is complimentary to the BamHI sites was then annealed to the circular cDNA (5’GTTCAGGATCCACGACGCTCTTCAAAA-3’) and the cDNA was re-linearised by digesting with BamHI. The resulting cDNA had 3’ adaptor and 5’ adaptor at the either ...
Quiz 9 BIol203 Fall 2013ppt
... Circle the portion of the above gene that you would use to make a transgene that expresses Arl in the leg only. 4pts. Mark with a bracket the portion of the above gene that you would use to make a transgene that expresses Arl in the antenna. 4pts. You want to insert a minimal promoter-Gal4pA transge ...
... Circle the portion of the above gene that you would use to make a transgene that expresses Arl in the leg only. 4pts. Mark with a bracket the portion of the above gene that you would use to make a transgene that expresses Arl in the antenna. 4pts. You want to insert a minimal promoter-Gal4pA transge ...
rNAi Biotechnology: Pros and Cons for Crop Improvement
... potential limitation arises from the fact that siRNAs comprise a population of molecules representing the entire sequence of the dsRNA trigger. Although this sequence heterogeneity could make it easy to silence a family of related genes with only one construct, it also opens the door to off-target e ...
... potential limitation arises from the fact that siRNAs comprise a population of molecules representing the entire sequence of the dsRNA trigger. Although this sequence heterogeneity could make it easy to silence a family of related genes with only one construct, it also opens the door to off-target e ...
Repeat-induced point mutation and the population
... rather suggest an effect of gene conversion, which is also a linkage-dependent process but results in a homogenization of repeated sequences. Particular sequences were found more widely distributed within the genome than expected by chance and may reflect the recently active variants. Therefore, seq ...
... rather suggest an effect of gene conversion, which is also a linkage-dependent process but results in a homogenization of repeated sequences. Particular sequences were found more widely distributed within the genome than expected by chance and may reflect the recently active variants. Therefore, seq ...
Identification of an Insertion Sequence Located
... Whether these sequences are part of an IS also in OF-positive strains is under investigation. Conclusions. In the AP1 strain of the M1 serotype, the sequence between the genes encoding protein SIC and the C5a peptidase was determined. It was found to contain an ORF with homology to six proteins enco ...
... Whether these sequences are part of an IS also in OF-positive strains is under investigation. Conclusions. In the AP1 strain of the M1 serotype, the sequence between the genes encoding protein SIC and the C5a peptidase was determined. It was found to contain an ORF with homology to six proteins enco ...
DNA replication
... During nuclear division the two strands of the DNA double helix separate through the action of enzyme DNA helicase, each DNA strand directing the synthesis of a complementary DNA strand through specific base pairing, resulting in two daughter DNA duplexes that are identical to the original parent mo ...
... During nuclear division the two strands of the DNA double helix separate through the action of enzyme DNA helicase, each DNA strand directing the synthesis of a complementary DNA strand through specific base pairing, resulting in two daughter DNA duplexes that are identical to the original parent mo ...
Sequence Analysis of the y-Globin Gene Locus from
... expression in the deletion form of HPFH described in American blacks (HPFH-1) is that the sequences juxtaposed to the y-globin genes as a result of the deletion contain an enhancer-like element that serves to maintain a transcriptionally active domain. A small fragment of D N A from the region immed ...
... expression in the deletion form of HPFH described in American blacks (HPFH-1) is that the sequences juxtaposed to the y-globin genes as a result of the deletion contain an enhancer-like element that serves to maintain a transcriptionally active domain. A small fragment of D N A from the region immed ...
Transposable element
A transposable element (TE or transposon) is a DNA sequence that can change its position within the genome, sometimes creating or reversing mutations and altering the cell's genome size. Transposition often results in duplication of the TE. Barbara McClintock's discovery of these jumping genes earned her a Nobel prize in 1983.TEs make up a large fraction of the C-value of eukaryotic cells. There are at least two classes of TEs: class I TEs generally function via reverse transcription, while class II TEs encode the protein transposase, which they require for insertion and excision, and some of these TEs also encode other proteins. It has been shown that TEs are important in genome function and evolution. In Oxytricha, which has a unique genetic system, they play a critical role in development. They are also very useful to researchers as a means to alter DNA inside a living organism.