lab6
... • In a species-independent manner., the GO project has developed three structured controlled vocabularies (ontologies) that describe gene products in terms of their associated ...
... • In a species-independent manner., the GO project has developed three structured controlled vocabularies (ontologies) that describe gene products in terms of their associated ...
Comparative study of overlapping genes in bacteria, with special
... (Table 1). The frequent occurrence of the unidirectional overlapping structure probably reflects the commonest orientation of adjacent genes in the chromosomes, as prokaryotic genes are often organized into operons or clusters of genes that are transcribed together. Since all genes in an operon must ...
... (Table 1). The frequent occurrence of the unidirectional overlapping structure probably reflects the commonest orientation of adjacent genes in the chromosomes, as prokaryotic genes are often organized into operons or clusters of genes that are transcribed together. Since all genes in an operon must ...
Supplementary material
... Close to 1,000 of the clones find multiple potential genes with our method. This often is a result of ESTs for the same clone belonging to different Unigene clusters, and can also be the result of closely related genes being found with the Blast method. In some cases, this automated method may not c ...
... Close to 1,000 of the clones find multiple potential genes with our method. This often is a result of ESTs for the same clone belonging to different Unigene clusters, and can also be the result of closely related genes being found with the Blast method. In some cases, this automated method may not c ...
Genome Sequence of an Extremely Halophilic Archaeon
... deoxyribonucleic acid (DNA) corresponded mainly to large heterogeneous extrachromosomal replicons containing many transposable insertion sequence (IS) elements (6). For Halobacterium NRC-1, extensive mapping revealed the presence of three replicons: pNRC100, about 200 kbp; pNRC200, nearly twice the ...
... deoxyribonucleic acid (DNA) corresponded mainly to large heterogeneous extrachromosomal replicons containing many transposable insertion sequence (IS) elements (6). For Halobacterium NRC-1, extensive mapping revealed the presence of three replicons: pNRC100, about 200 kbp; pNRC200, nearly twice the ...
Cell-Specific Expression of Genes of the Lipid Transfer Protein
... synthase; PAL, phenylalanine ammonia lyase. ...
... synthase; PAL, phenylalanine ammonia lyase. ...
Murine herpesvirus 68 is genetically related to the
... properties of a virus are ultimately determined by its genetic content, it follows that the members of any one subgroup will be genetically related. However, the limitations of using subjective biological criteria for the classification of herpesviruses are best illustrated by considering Marek's di ...
... properties of a virus are ultimately determined by its genetic content, it follows that the members of any one subgroup will be genetically related. However, the limitations of using subjective biological criteria for the classification of herpesviruses are best illustrated by considering Marek's di ...
Bioinformatics - Sequences and Computers
... contain information that is realized by readers, the sequence of nucleotides in DNA contains information that is realized by the gene expression machinery of cells. Just as documents may provide information that stimulates readers to act on something (like adopting a new life style), genes contain t ...
... contain information that is realized by readers, the sequence of nucleotides in DNA contains information that is realized by the gene expression machinery of cells. Just as documents may provide information that stimulates readers to act on something (like adopting a new life style), genes contain t ...
Analysis of a genomic segment of white spot syndrome virus of
... was identical to the sequence in the 11n3 kb BamHI fragment, confirming the overlap between these two fragments. To identify the 3h end of the rr2 ORF, a 3h RACE analysis was performed on RNA isolated from infected shrimp tissue (data not shown). The ORF is 1242 nucleotides long and potentially enco ...
... was identical to the sequence in the 11n3 kb BamHI fragment, confirming the overlap between these two fragments. To identify the 3h end of the rr2 ORF, a 3h RACE analysis was performed on RNA isolated from infected shrimp tissue (data not shown). The ORF is 1242 nucleotides long and potentially enco ...
Developing a CRISPR/Cas9 System for Volvox Carteri
... than previously developed genome editing systems. The high precision is due to the CRISPR associated (Cas) endonuclease’s ability to bind DNA via associated guide RNAs. Cas endonucleases can delete or add bases to the genome, which permits not only knockouts to determine mutant phenotypes, but also ...
... than previously developed genome editing systems. The high precision is due to the CRISPR associated (Cas) endonuclease’s ability to bind DNA via associated guide RNAs. Cas endonucleases can delete or add bases to the genome, which permits not only knockouts to determine mutant phenotypes, but also ...
File formats for NGS data - Bioinformatics Training Materials
... ● Allows for genes and genomic features to be evaluated in their genomic context. ○ Gene A is close to gene B ○ Gene A and gene B are within feature C ● Can be used to align shallow targeted high-throughput sequencing to a pre-built map of an organism ...
... ● Allows for genes and genomic features to be evaluated in their genomic context. ○ Gene A is close to gene B ○ Gene A and gene B are within feature C ● Can be used to align shallow targeted high-throughput sequencing to a pre-built map of an organism ...
Chromosomal rearrangements in Salmonella spp. s2-2
... mologous recombination, resulting in deletions29; site-specific recombination at the dif-site in the TER region, due to activity of two related recombinases XerC and XerD of the lambda integrase family30. 11 is likely that these types of recombination are responsible for inversion in wild type strai ...
... mologous recombination, resulting in deletions29; site-specific recombination at the dif-site in the TER region, due to activity of two related recombinases XerC and XerD of the lambda integrase family30. 11 is likely that these types of recombination are responsible for inversion in wild type strai ...
Part 2 - Laboratory of Aquaculture & Artemia Reference Center
... level approaches are yet to be iniated such as functional genomics, population genomics etc. Artemia shows well developed gene regulatory system for its survival even under extreme conditions. Artemia seems to be a promising system for stress response studies as observed in our laboratory since it s ...
... level approaches are yet to be iniated such as functional genomics, population genomics etc. Artemia shows well developed gene regulatory system for its survival even under extreme conditions. Artemia seems to be a promising system for stress response studies as observed in our laboratory since it s ...
Sequence and transcription analysis of the Petunia mitochondrial
... indicated in Fig. 2 ) . Shorter bands are likely due to RNA breakdown as their location and quantity varies with different RNA preparations and time in storage. ...
... indicated in Fig. 2 ) . Shorter bands are likely due to RNA breakdown as their location and quantity varies with different RNA preparations and time in storage. ...
Genomic Structure of the Human IgX1 Gene Suggests That It May
... fragment diagnostic of 14 .1 and was not characterized further. The remaining two clones contained a 3 .0-kb BamHI fragment that hybridized to the 14.1 exon 1 probe and restriction map analysis indicated that the two clones shared common sequence . One of these clones, GA1, was analyzed in more det ...
... fragment diagnostic of 14 .1 and was not characterized further. The remaining two clones contained a 3 .0-kb BamHI fragment that hybridized to the 14.1 exon 1 probe and restriction map analysis indicated that the two clones shared common sequence . One of these clones, GA1, was analyzed in more det ...
Genetic Linkage Mapping of Zebrafish Genes and
... panel and in previous work. The HS panel map assigned 591 of 593 SSLPs to the same linkage groups as Shimoda et al. (1999). Of these 591 markers, there were eight cases where the order of closely spaced markers differed between the two maps. Because closely spaced markers are separated by few recomb ...
... panel and in previous work. The HS panel map assigned 591 of 593 SSLPs to the same linkage groups as Shimoda et al. (1999). Of these 591 markers, there were eight cases where the order of closely spaced markers differed between the two maps. Because closely spaced markers are separated by few recomb ...
A Tn 10-lacZ-kanR-URA3 Gene Fusion Transposon for Insertion Mutagenesis and Fusion Analysis of Yeast and Bacterial Genes.
... E. coli strain harboring the desired pBR322-derived target plasmid and a compatible pACYC 184 plasmid that produces T n 10 transposase protein. When infected cells are plated on kanamycin selective medium, KanR colonies arise at the frequency of about 1 per lo4 infecting phage genomes. Each such Kan ...
... E. coli strain harboring the desired pBR322-derived target plasmid and a compatible pACYC 184 plasmid that produces T n 10 transposase protein. When infected cells are plated on kanamycin selective medium, KanR colonies arise at the frequency of about 1 per lo4 infecting phage genomes. Each such Kan ...
FAN: fingerprint analysis of nucleotide sequences
... E-value of one for a database the size of PRINTS—not surprisingly, the fingerprint is unrelated. The sequence shown in the figure has a frameshift-causing sequence error; however, because it does not occur within a motif-matching region, it has had no effect on the significance of the match. The rel ...
... E-value of one for a database the size of PRINTS—not surprisingly, the fingerprint is unrelated. The sequence shown in the figure has a frameshift-causing sequence error; however, because it does not occur within a motif-matching region, it has had no effect on the significance of the match. The rel ...
Full Text - Genes | Genomes | Genetics
... DNA/RNA metabolism, protein folding, trafficking, and degradation; Chervitz et al. 1998). The major limitation of this study was that yeast and worm were the only complete eukaryotic genome sequences available at the time. Today, hundreds of diverse genomes have been sequenced. However, not all these ...
... DNA/RNA metabolism, protein folding, trafficking, and degradation; Chervitz et al. 1998). The major limitation of this study was that yeast and worm were the only complete eukaryotic genome sequences available at the time. Today, hundreds of diverse genomes have been sequenced. However, not all these ...
Pseudogene function: regulation of gene expression
... A second functional (pseudo)gene, occurring in tandem with the first one (see Figure 2) is now known to exist: ‘Note that in antiNOS-1 the antisense region is located at the 5’ end of the molecule, whereas in antiNOS-2 it is located at the 3’ end. Another important difference is that although antiNOS ...
... A second functional (pseudo)gene, occurring in tandem with the first one (see Figure 2) is now known to exist: ‘Note that in antiNOS-1 the antisense region is located at the 5’ end of the molecule, whereas in antiNOS-2 it is located at the 3’ end. Another important difference is that although antiNOS ...
Evolution of bacterial resistance to antibiotics during the last three
... of the aadA and aacC1 genes stands out, which implies crossresistance on the one hand to streptomycin and spectinomycin and on the other to gentamicin, sisomicin and fortimicin. The stable position of the resistance genes in an integron, present in an R plasmid, assures their dissemination and the c ...
... of the aadA and aacC1 genes stands out, which implies crossresistance on the one hand to streptomycin and spectinomycin and on the other to gentamicin, sisomicin and fortimicin. The stable position of the resistance genes in an integron, present in an R plasmid, assures their dissemination and the c ...
msb4100030-sup
... Orthologous human and mouse proliferation cluster genes preserve the composition of the regulatory motifs in their promoters. Distribution of motif compositional similarity of orthologous human – mouse promoters from the proliferation cluster, and random human – mouse gene pairs. Given the five moti ...
... Orthologous human and mouse proliferation cluster genes preserve the composition of the regulatory motifs in their promoters. Distribution of motif compositional similarity of orthologous human – mouse promoters from the proliferation cluster, and random human – mouse gene pairs. Given the five moti ...
The genomes of four tapeworm species reveal adaptations to
... 3.1, 3.2 and 3.3), consistent with previous observations of karyotype plasticity in flatworms11. Aided by deep transcriptome sequencing from multiple life-cycle stages, we identified 10,231 to 12,490 putative genes per genome (Supplementary Table 5.5). Similar to the genome of S. mansoni12, distinct ...
... 3.1, 3.2 and 3.3), consistent with previous observations of karyotype plasticity in flatworms11. Aided by deep transcriptome sequencing from multiple life-cycle stages, we identified 10,231 to 12,490 putative genes per genome (Supplementary Table 5.5). Similar to the genome of S. mansoni12, distinct ...
12-Transcription-The Relationship Between Genes and Proteins
... • RNA polymerase binds to different promoters with different strengths; binding strength relates to the level of gene expression • There are some common consensus sequences for promoters: ...
... • RNA polymerase binds to different promoters with different strengths; binding strength relates to the level of gene expression • There are some common consensus sequences for promoters: ...
Felis domesticus papillomavirus, isolated from a skin lesion, is
... 1994), among others. The FdPV NCR-1 contains a variety of putative regulatory elements, although the FdPV NCR-1 does not include a TATA box within the E6\E7 promoter region. However, multiple SP-1 binding sites were identified that could serve as the transcription initiation site (Peterson et al., 1 ...
... 1994), among others. The FdPV NCR-1 contains a variety of putative regulatory elements, although the FdPV NCR-1 does not include a TATA box within the E6\E7 promoter region. However, multiple SP-1 binding sites were identified that could serve as the transcription initiation site (Peterson et al., 1 ...
Multiple Choice - Test Bank Team
... molecule could be laid end to end, how long would it be? The distance between neighboring base pairs in DNA is typically around 0.34 nm. A. About 7 mm B. About 7 cm C. About 70 cm D. About 7 m E. None of the above ...
... molecule could be laid end to end, how long would it be? The distance between neighboring base pairs in DNA is typically around 0.34 nm. A. About 7 mm B. About 7 cm C. About 70 cm D. About 7 m E. None of the above ...
Transposable element
A transposable element (TE or transposon) is a DNA sequence that can change its position within the genome, sometimes creating or reversing mutations and altering the cell's genome size. Transposition often results in duplication of the TE. Barbara McClintock's discovery of these jumping genes earned her a Nobel prize in 1983.TEs make up a large fraction of the C-value of eukaryotic cells. There are at least two classes of TEs: class I TEs generally function via reverse transcription, while class II TEs encode the protein transposase, which they require for insertion and excision, and some of these TEs also encode other proteins. It has been shown that TEs are important in genome function and evolution. In Oxytricha, which has a unique genetic system, they play a critical role in development. They are also very useful to researchers as a means to alter DNA inside a living organism.