Proteorhodopsin Phototrophy Promotes Survival of Marine
... Notably, epifluorescence microscopy images of AND4 cultures showed that most of the observed increase in cell numbers was due to reductive division rather than growth, i.e., cell numbers increased, but total biomass did not because each cell decreased in size (Figure 3B). This decrease in cell size ...
... Notably, epifluorescence microscopy images of AND4 cultures showed that most of the observed increase in cell numbers was due to reductive division rather than growth, i.e., cell numbers increased, but total biomass did not because each cell decreased in size (Figure 3B). This decrease in cell size ...
digital PCR - Bio-Rad
... phenotypic traits and disease susceptibility (Beckmann et al. 2008). The advent of holistic discovery methods afforded by DNA microarrays (Redon et al. 2006) and more recently by next-generation DNA sequencing (Sudmant et al. 2010) have allowed us to better understand the extent of genetic variation ...
... phenotypic traits and disease susceptibility (Beckmann et al. 2008). The advent of holistic discovery methods afforded by DNA microarrays (Redon et al. 2006) and more recently by next-generation DNA sequencing (Sudmant et al. 2010) have allowed us to better understand the extent of genetic variation ...
Synthesis, identification and thermal decomposition of double
... Double sul®tes with empirical formula Cu2SO3MSO32H2O (where M is Cu, Fe, Mn, or Cd) were obtained by saturation with sulfur dioxide gas of an aqueous mixture of MII sulfate and copper sulfate at room temperature. The salts obtained were identi®ed by infrared spectra, X-ray powder diffraction and e ...
... Double sul®tes with empirical formula Cu2SO3MSO32H2O (where M is Cu, Fe, Mn, or Cd) were obtained by saturation with sulfur dioxide gas of an aqueous mixture of MII sulfate and copper sulfate at room temperature. The salts obtained were identi®ed by infrared spectra, X-ray powder diffraction and e ...
Michigan State University Plant Genomics Program
... RNA would be under no stress conditions for DDF1 and DDF2. • Since even moving the plants between the filter paper and the plate to the 50mL reaction tube could cause some form of wounding to the small seedlings, to eliminate the chances of RNA levels changing because of those minor stresses, we are ...
... RNA would be under no stress conditions for DDF1 and DDF2. • Since even moving the plants between the filter paper and the plate to the 50mL reaction tube could cause some form of wounding to the small seedlings, to eliminate the chances of RNA levels changing because of those minor stresses, we are ...
Supplementary Information
... PCR using primers LacZ-A_EcoRI and 35S-Z_XbaI. The other CaMV 35S promoter was prepared from pCAMBIA1301 by PCR using primers 35S-D_EcoRI and 35S-Z_KpnI. These fragments were inserted between KpnI and XbaI sites of the pCAM-attR (pDual35SGW1301). The fragment including CaMV 35S promoter regions and ...
... PCR using primers LacZ-A_EcoRI and 35S-Z_XbaI. The other CaMV 35S promoter was prepared from pCAMBIA1301 by PCR using primers 35S-D_EcoRI and 35S-Z_KpnI. These fragments were inserted between KpnI and XbaI sites of the pCAM-attR (pDual35SGW1301). The fragment including CaMV 35S promoter regions and ...
Whole Exome Sequencing
... – Includes sequence of the genes – exons & introns – Includes sequence of regions between genes ...
... – Includes sequence of the genes – exons & introns – Includes sequence of regions between genes ...
The Complete Chloroplast and Mitochondrial DNA Sequence of
... variation in gene content, due to massive gene loss, genome erosion, and gene transfer to the nucleus (Grzebyk and Schofield 2003). All cp genomes described so far are circular. Previous studies have shown that, although in green algae (e.g., C. reinhardtii) more genes have been transferred to the n ...
... variation in gene content, due to massive gene loss, genome erosion, and gene transfer to the nucleus (Grzebyk and Schofield 2003). All cp genomes described so far are circular. Previous studies have shown that, although in green algae (e.g., C. reinhardtii) more genes have been transferred to the n ...
Intra-isolate genome variation in arbuscular mycorrhizal
... If PCR is performed on a mixed template, in vitro recombination can bias polymorphism estimates. This is especially relevant when using Pfu polymerases and a higher number of PCR cycles (between 20 and 40) (Zylstra et al., 1998). The heterokaryotic state of the AMF under study would provide a mixed ...
... If PCR is performed on a mixed template, in vitro recombination can bias polymorphism estimates. This is especially relevant when using Pfu polymerases and a higher number of PCR cycles (between 20 and 40) (Zylstra et al., 1998). The heterokaryotic state of the AMF under study would provide a mixed ...
Case Study: Visualization of annotated DNA sequences
... C++ and runs on both Windows and Linux platforms. Figure 1 shows a screen shot of DNAVis. The largest part of the screen is taken by a canvas. The canvas contains one or more views. In each view one or more data sets are visualized. Figure 1 shows four views: three bar views and one matrix view. The ...
... C++ and runs on both Windows and Linux platforms. Figure 1 shows a screen shot of DNAVis. The largest part of the screen is taken by a canvas. The canvas contains one or more views. In each view one or more data sets are visualized. Figure 1 shows four views: three bar views and one matrix view. The ...
Simple Sequence Repeats as Advantageous Mutators
... regulatory region of this gene, much of which is absent in the two asocial species. (Interestingly, bonobos (Pan paniscus) and humans, two primate species characterized by high empathic and sexual bonding, also share a highly homologous SSR-rich tract upstream of the avpr1a gene, while the correspon ...
... regulatory region of this gene, much of which is absent in the two asocial species. (Interestingly, bonobos (Pan paniscus) and humans, two primate species characterized by high empathic and sexual bonding, also share a highly homologous SSR-rich tract upstream of the avpr1a gene, while the correspon ...
Developing codominant PCR markers in pines
... revealing few RFLP bands, but we also selected a few clones with more complex banding patterns (e.g. Gerttula and Kinlaw, 1993). After selecting the clones, we determined the nucleotide sequence for 150-250 bases into each end of the cloned inserts (i.e. one sequencing reaction and gel, using stand ...
... revealing few RFLP bands, but we also selected a few clones with more complex banding patterns (e.g. Gerttula and Kinlaw, 1993). After selecting the clones, we determined the nucleotide sequence for 150-250 bases into each end of the cloned inserts (i.e. one sequencing reaction and gel, using stand ...
Dow, Graham: The limitation of genome wide association studies
... variants that are commonplace are the result of mutations that took place many generations ago, and spread throughout human genealogy either through genetic drift or selection. Rare SNPs, on the other hand, have arisen from recent mutations, even some within ...
... variants that are commonplace are the result of mutations that took place many generations ago, and spread throughout human genealogy either through genetic drift or selection. Rare SNPs, on the other hand, have arisen from recent mutations, even some within ...
Nongenic transcription, gene regulation and action at a distance
... but not other genes with powerful upstream activation sequences (e.g. TEF1 and TEF2) (Bi and Broach, 1999), CHA1 – which flanks the HML mating-type locus – becomes a robust barrier when induced by serine (Donze and Kamakaka, 2001), and inverting the β-globin LCR destroys much of its activity (Tanimo ...
... but not other genes with powerful upstream activation sequences (e.g. TEF1 and TEF2) (Bi and Broach, 1999), CHA1 – which flanks the HML mating-type locus – becomes a robust barrier when induced by serine (Donze and Kamakaka, 2001), and inverting the β-globin LCR destroys much of its activity (Tanimo ...
Saccharomyces Genome Database (SGD) provides secondary gene annotation using the Gene Ontology (GO).
... genes to GO will allow users to find all genes, including those across species, which share the same (or related) annotation(s) for function, process and component. GO consists of three ontologies, representing the fundamental aspects of gene products: molecular function, biological process and cell ...
... genes to GO will allow users to find all genes, including those across species, which share the same (or related) annotation(s) for function, process and component. GO consists of three ontologies, representing the fundamental aspects of gene products: molecular function, biological process and cell ...
Insertion of liver enriched transcription
... regulatory system. Tissue-specific promoters or enhancers are in use in transgenic animals and could be utilized in medicine for gene therapy. At present the usual method for selection of a tissue-specific promoter is to identify a gene, which is expressed at unusually high level in the target tissu ...
... regulatory system. Tissue-specific promoters or enhancers are in use in transgenic animals and could be utilized in medicine for gene therapy. At present the usual method for selection of a tissue-specific promoter is to identify a gene, which is expressed at unusually high level in the target tissu ...
Full Text - The International Journal of Developmental Biology
... interact much in the same way as sog and dpp do to establish the dorso-ventral axis of the embryo (Sasai et al., 1994,1995; Holley et al., 1995; Schmidt et al., 1995). Interestingly, however, the D/V axis as defined by the domains of activity of chordin and BMP4 in vertebrate is inverted relative to ...
... interact much in the same way as sog and dpp do to establish the dorso-ventral axis of the embryo (Sasai et al., 1994,1995; Holley et al., 1995; Schmidt et al., 1995). Interestingly, however, the D/V axis as defined by the domains of activity of chordin and BMP4 in vertebrate is inverted relative to ...
Title: Genes in the Postgenomic Era Authors: Paul E. Griffiths and
... over during meiosis - is a process that recombines whole genes. However, if recombination can occur within a gene, so that part of the gene on one chromosome comes to be united with part of the same gene from the other homologous chromosome, then it is possible for the cis-trans test to fail. Intrag ...
... over during meiosis - is a process that recombines whole genes. However, if recombination can occur within a gene, so that part of the gene on one chromosome comes to be united with part of the same gene from the other homologous chromosome, then it is possible for the cis-trans test to fail. Intrag ...
The Evolution of tRNA-Leu Genes in Animal
... the PHASE MCMC program. The L and L2 genes are split into two well-resolved clusters with 100% posterior probability, with the exception that the L gene of P. longicarpus appears in the middle of the L2 gene cluster, very close to the L2 gene of the same species. This is precisely what we would expe ...
... the PHASE MCMC program. The L and L2 genes are split into two well-resolved clusters with 100% posterior probability, with the exception that the L gene of P. longicarpus appears in the middle of the L2 gene cluster, very close to the L2 gene of the same species. This is precisely what we would expe ...
Recurrence time statistics: Versatile tools for genomic DNA
... underlying those variations. Equally or even more important, repeat-related features often have to be masked before protein coding regions along a DNA sequence are to be identified or redundant expressed sequence tags (ESTs) are to be sequenced. More important than finding repeat-related structures ...
... underlying those variations. Equally or even more important, repeat-related features often have to be masked before protein coding regions along a DNA sequence are to be identified or redundant expressed sequence tags (ESTs) are to be sequenced. More important than finding repeat-related structures ...
Slide 1
... physical genetic material be What methods would you use? disadvantageous for fern evolution? Could it be related to slow speciation 2. Why is the fate of most duplicate genes to rates, compared to angiosperms? Or, on eventually become silenced? Could the other hand, could the silenced genes mutation ...
... physical genetic material be What methods would you use? disadvantageous for fern evolution? Could it be related to slow speciation 2. Why is the fate of most duplicate genes to rates, compared to angiosperms? Or, on eventually become silenced? Could the other hand, could the silenced genes mutation ...
Chapter 12
... non-LTR retrotransposons no long terminal repeats long interspersed elements short interspersed elements Alu I family 300 bp 106 copies 11% Human DNA ...
... non-LTR retrotransposons no long terminal repeats long interspersed elements short interspersed elements Alu I family 300 bp 106 copies 11% Human DNA ...
BLAST- bioinformatics
... domains like catalytic sites, DNA binding regions etc.) are likely to display different SUbstitution probabilities. ...
... domains like catalytic sites, DNA binding regions etc.) are likely to display different SUbstitution probabilities. ...
View Poster - Technology Networks
... Potential targets for four out of 18 tested candidate miRNAs could be verified by 5’ RACE analysis, showing cleavage in the centre of the predicted miRNA target site. This is similar to what is found in most miRNA targets of higher plants Two verified targets are associated with cell motility, one i ...
... Potential targets for four out of 18 tested candidate miRNAs could be verified by 5’ RACE analysis, showing cleavage in the centre of the predicted miRNA target site. This is similar to what is found in most miRNA targets of higher plants Two verified targets are associated with cell motility, one i ...
Transposable element
A transposable element (TE or transposon) is a DNA sequence that can change its position within the genome, sometimes creating or reversing mutations and altering the cell's genome size. Transposition often results in duplication of the TE. Barbara McClintock's discovery of these jumping genes earned her a Nobel prize in 1983.TEs make up a large fraction of the C-value of eukaryotic cells. There are at least two classes of TEs: class I TEs generally function via reverse transcription, while class II TEs encode the protein transposase, which they require for insertion and excision, and some of these TEs also encode other proteins. It has been shown that TEs are important in genome function and evolution. In Oxytricha, which has a unique genetic system, they play a critical role in development. They are also very useful to researchers as a means to alter DNA inside a living organism.