Functional Analysis of the Genes of Yeast Chromosome V by Genetic Footprinting.
... time-zero population). This category includes genes whose product is absolutely required for vegetative cell growth (“essential” genes). The Q2 and Q3 categories include genes for which mutant cells were at more subtle growth disadvantages, growing at apparent rates of 75 to 85% and 85 to ,100% of ...
... time-zero population). This category includes genes whose product is absolutely required for vegetative cell growth (“essential” genes). The Q2 and Q3 categories include genes for which mutant cells were at more subtle growth disadvantages, growing at apparent rates of 75 to 85% and 85 to ,100% of ...
IOSR Journal of Dental and Medical Sciences (IOSR-JDMS)
... male births [3-5] caused by mutations in the gene, DMD (chromosomal locus Xp21.3-p21.2), that encodes the protein, dystrophin. The dystrophin gene has been identified by positional cloning in 1986 on chromosome X [6, 7]. With 79 exons and 8 promoters and consisting of 2.4 million base pairs, the DMD ...
... male births [3-5] caused by mutations in the gene, DMD (chromosomal locus Xp21.3-p21.2), that encodes the protein, dystrophin. The dystrophin gene has been identified by positional cloning in 1986 on chromosome X [6, 7]. With 79 exons and 8 promoters and consisting of 2.4 million base pairs, the DMD ...
Hox Genes in Development and Disease – Lecture 2
... Hand-Foot-Genital syndrome Hand-Foot-Genital syndrome is a dominant hereditary disease caused by nonsense mutations that truncate Hoxa 13 in the DNA-binding helix. In HandFoot-Genital syndrome males may have hypospadias, and females defects in uterine septation and urethral malformations. The thumb ...
... Hand-Foot-Genital syndrome Hand-Foot-Genital syndrome is a dominant hereditary disease caused by nonsense mutations that truncate Hoxa 13 in the DNA-binding helix. In HandFoot-Genital syndrome males may have hypospadias, and females defects in uterine septation and urethral malformations. The thumb ...
Slide 1
... Evidence for the RNAi pathway activity DICER in T. reesei Small interfering RNAs corresponding to the al-1 dsRNA.The transformants 1, 24 and 42 show a clear accumulation of siRNA. The RNA was extracted from cultures either in quinic induced (i) or noninduced conditions (ni). The 6xw is a Neurospora ...
... Evidence for the RNAi pathway activity DICER in T. reesei Small interfering RNAs corresponding to the al-1 dsRNA.The transformants 1, 24 and 42 show a clear accumulation of siRNA. The RNA was extracted from cultures either in quinic induced (i) or noninduced conditions (ni). The 6xw is a Neurospora ...
Slide
... If read r overlaps to the right reads r1, r2, and r1 overlaps r2, then (r, r2) can be inferred by (r, r1) and (r1, r2) ...
... If read r overlaps to the right reads r1, r2, and r1 overlaps r2, then (r, r2) can be inferred by (r, r1) and (r1, r2) ...
Microarray statistical validation and functional annotation
... a pathological condition – to rule out molecular mechanisms tightly bound to transcription ...
... a pathological condition – to rule out molecular mechanisms tightly bound to transcription ...
Precise insertion and guided editing of higher plant
... hygromycin resistance gene under the control of the maize ubiquitin promoter flanked by 1kb regions of rice genomic DNA known as homology arms (Table S1-2). The Cpf1 genes were codon optimized for monocot plants, including an N-terminal nuclear localization tag, and were expressed by the 2x35S CaMV ...
... hygromycin resistance gene under the control of the maize ubiquitin promoter flanked by 1kb regions of rice genomic DNA known as homology arms (Table S1-2). The Cpf1 genes were codon optimized for monocot plants, including an N-terminal nuclear localization tag, and were expressed by the 2x35S CaMV ...
A P element-homologous sequence in the house fly, Musca domestica
... analysis of a genomic clone (Md-P1) indicates that, although the house fly P element has lost its coding capacity, the basic general structure of drosophilid P elements is present. The house fly P element sequence shares a number of structural features with that from the blow fly, Lucilia cuprina, i ...
... analysis of a genomic clone (Md-P1) indicates that, although the house fly P element has lost its coding capacity, the basic general structure of drosophilid P elements is present. The house fly P element sequence shares a number of structural features with that from the blow fly, Lucilia cuprina, i ...
The Chlamydomonas genome project: a decade on
... As a unicellular haploid in the vegetative stage of its life cycle, Chlamydomonas shares the experimental advantages associated with microbes. These include rapid doubling time (8–12 h), well-defined media and growth requirements, the ability to synchronize cultures with periodic light exposure, th ...
... As a unicellular haploid in the vegetative stage of its life cycle, Chlamydomonas shares the experimental advantages associated with microbes. These include rapid doubling time (8–12 h), well-defined media and growth requirements, the ability to synchronize cultures with periodic light exposure, th ...
Structure and evolution of Apetala3, a sex
... is formed by a 6 bp long direct repeat. Both ends of the repeat are bordered by inverted tandem structures that resemble the organization of a MITE element (Additional file 4, Figure S4). GenBank database searches revealed no similarity of this part of the promoter to any known sequence, except for ...
... is formed by a 6 bp long direct repeat. Both ends of the repeat are bordered by inverted tandem structures that resemble the organization of a MITE element (Additional file 4, Figure S4). GenBank database searches revealed no similarity of this part of the promoter to any known sequence, except for ...
Phylogenetic Portrait of the Saccharomyces cerevisiae Functional
... DNA/RNA metabolism, protein folding, trafficking, and degradation; Chervitz et al. 1998). The major limitation of this study was that yeast and worm were the only complete eukaryotic genome sequences available at the time. Today, hundreds of diverse genomes have been sequenced. However, not all these ...
... DNA/RNA metabolism, protein folding, trafficking, and degradation; Chervitz et al. 1998). The major limitation of this study was that yeast and worm were the only complete eukaryotic genome sequences available at the time. Today, hundreds of diverse genomes have been sequenced. However, not all these ...
Supporting Online Material
... animals having undergone puparium formation in certain time points are shown in Figure 3B. To stage the silenced and non-silenced pupae, wandering larvae were collected from the wall of the tubes. GFP-expression was determined on living animals using a Leica DMLS fluorescence microscope. Silenced an ...
... animals having undergone puparium formation in certain time points are shown in Figure 3B. To stage the silenced and non-silenced pupae, wandering larvae were collected from the wall of the tubes. GFP-expression was determined on living animals using a Leica DMLS fluorescence microscope. Silenced an ...
Model for transcriptional activation
... are recognized by RNA Pol I, II, and III, respectively. • Class I promoters not well conserved in sequence amongst different species but general architecture consists of a core element and an upstream promoter element (UPE). • Some Class III promoters have promoter sequences wholly within the gene. ...
... are recognized by RNA Pol I, II, and III, respectively. • Class I promoters not well conserved in sequence amongst different species but general architecture consists of a core element and an upstream promoter element (UPE). • Some Class III promoters have promoter sequences wholly within the gene. ...
- Murdoch Research Repository
... random 7.5 kb fragments of host DNA between B. hyodysenteriae cells (Matson et al., 2007). Analysis of VSH-1 in B. hyodysenteriae strain B204 has shown that it is located in a 16.3 kb region of the genome, and includes three “modules” or sets of operons of late function genes encoding the prophage c ...
... random 7.5 kb fragments of host DNA between B. hyodysenteriae cells (Matson et al., 2007). Analysis of VSH-1 in B. hyodysenteriae strain B204 has shown that it is located in a 16.3 kb region of the genome, and includes three “modules” or sets of operons of late function genes encoding the prophage c ...
Editing of a tRNA anticodon in marsupial
... RNA editing is a process by which RNA is post-transciptionally changed such that new genetic information not encoded in the DNA is created. In mitochondria of protozoa RNA editing results in the insertion and deletion of undine residues (1) and thus extensive modifications of many mRNAs (2). In plan ...
... RNA editing is a process by which RNA is post-transciptionally changed such that new genetic information not encoded in the DNA is created. In mitochondria of protozoa RNA editing results in the insertion and deletion of undine residues (1) and thus extensive modifications of many mRNAs (2). In plan ...
Identification of incomplete coding sequences for
... inserts from positive plaques have been isolated and subcloned into the vector pUC18 (Ballabio et al. 1987). One of these clones, p422. contains a 560bp insert. However later studies have shown that only 2(X) bp of this clone correspond to the cDN A for STS. the remaining sequence being of unknown o ...
... inserts from positive plaques have been isolated and subcloned into the vector pUC18 (Ballabio et al. 1987). One of these clones, p422. contains a 560bp insert. However later studies have shown that only 2(X) bp of this clone correspond to the cDN A for STS. the remaining sequence being of unknown o ...
Lab 5: IDENTIFICATION OF UNKNOWN MICROORGANISMS
... Large subunit of ribosome Small subunit of ribosome Large subunit of ribosome ...
... Large subunit of ribosome Small subunit of ribosome Large subunit of ribosome ...
Predicting TF affinities to Promoters of tissue specific genes
... TF ranking for SRF target genes In Section 4.2.2 it was shown that for a given intergenic region in yeast TRAP can oftentimes successfully predict the corresponding regulating TF by ranking all PFMs according to their predicted affinities for the region. Here I address the question of whether TRAP ...
... TF ranking for SRF target genes In Section 4.2.2 it was shown that for a given intergenic region in yeast TRAP can oftentimes successfully predict the corresponding regulating TF by ranking all PFMs according to their predicted affinities for the region. Here I address the question of whether TRAP ...
linkage-recomb2
... Know the effect that crossing over has on distantly located genes. Know what cross-over values (COV) are. ...
... Know the effect that crossing over has on distantly located genes. Know what cross-over values (COV) are. ...
Diapositiva 1
... A non-redundant collection of richly annotated DNA, RNA, and protein sequences from diverse taxa The collection includes sequences from plasmids, organelles, viruses, archaea, bacteria, and eukaryotes Each RefSeq represents a single, naturally occurring molecule from one organism. RefSeq biological ...
... A non-redundant collection of richly annotated DNA, RNA, and protein sequences from diverse taxa The collection includes sequences from plasmids, organelles, viruses, archaea, bacteria, and eukaryotes Each RefSeq represents a single, naturally occurring molecule from one organism. RefSeq biological ...
Draft of first homework
... The human DICER1 gene encodes an important ribonuclease, involved in miRNA and siRNA processing. Several mRNAs representing this gene have been mapped to the human genome (March 2006 assembly). We will look closer at one of them: AK002007. What are the first five nucleotides from the first inferred ...
... The human DICER1 gene encodes an important ribonuclease, involved in miRNA and siRNA processing. Several mRNAs representing this gene have been mapped to the human genome (March 2006 assembly). We will look closer at one of them: AK002007. What are the first five nucleotides from the first inferred ...
Ribosome reinitiation at leader peptides increases translation of
... [7]. Taxonomic groups Cyanobacteria, Tenericutes, and Firmicutes, which had no peak or not very pronounced peak, represent early diverged bacterial branches [15, 16]. In addition, there is no reliable data on the presence of attenuation mechanism in cyanobacteria and tenericutes. This also agrees wi ...
... [7]. Taxonomic groups Cyanobacteria, Tenericutes, and Firmicutes, which had no peak or not very pronounced peak, represent early diverged bacterial branches [15, 16]. In addition, there is no reliable data on the presence of attenuation mechanism in cyanobacteria and tenericutes. This also agrees wi ...
Transposable element
A transposable element (TE or transposon) is a DNA sequence that can change its position within the genome, sometimes creating or reversing mutations and altering the cell's genome size. Transposition often results in duplication of the TE. Barbara McClintock's discovery of these jumping genes earned her a Nobel prize in 1983.TEs make up a large fraction of the C-value of eukaryotic cells. There are at least two classes of TEs: class I TEs generally function via reverse transcription, while class II TEs encode the protein transposase, which they require for insertion and excision, and some of these TEs also encode other proteins. It has been shown that TEs are important in genome function and evolution. In Oxytricha, which has a unique genetic system, they play a critical role in development. They are also very useful to researchers as a means to alter DNA inside a living organism.