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Biotechnology
Biotechnology

... • What are they? Blank copies of cells that are not specialized • What can we use them for? To grow new organs/tissues, ...
Genetic Engineering (and other cool molecular biology techniques)
Genetic Engineering (and other cool molecular biology techniques)

... Genetic Engineering (and other cool molecular biology techniques) ...
view PDF - Children`s Hospital of Wisconsin
view PDF - Children`s Hospital of Wisconsin

... to make proteins; it is the proteins that carry out the functions of the cell. Change in genes cause genetic disorders. For example, changes in one gene give rise to cystic fibrosis (CFTR) while changes in other genes can result in breast and ovarian cancer (BRCA1) or affect drug metabolism (CYP2C9) ...
Handout 2: Glossary
Handout 2: Glossary

... keto form A form of guanine or thymine in which a hydrogen atom bonds to a nitrogen atom within the nitrogen ring of the base. nitrogenous base One of four nitrogen containing bases - adenine, thymine, guanine, and cytosine - that make up nucleotides. nucleic acid An acid compound, such as DNA or RN ...
Science and Society: Unit 2 Review Packet Directions: Use your
Science and Society: Unit 2 Review Packet Directions: Use your

Human Genome Project
Human Genome Project

... Human Genome Project (HGP) Who did the work? The work begun formally in 1990, carried out in 16 centers across the world. The project originally was planned to last 15 years, but rapid technological advances have accelerated the expected completion date to 2003. The international Human Genome Mappi ...
Chapter 19 – Molecular Genetic Analysis and Biotechnology
Chapter 19 – Molecular Genetic Analysis and Biotechnology

... • Make double-stranded cuts in DNA • Bacterial source – guards against viral invasion – Bacterial DNA is methylated; viral unmethylated ...
221_exam_2_2002
221_exam_2_2002

... Escherichia coli that has been responsible for several outbreaks in south central Missouri. He has identified a cluster of genes that seems to be responsible for the virulent nature of this new isolate. One significant discovery he has made is that the G+C ratio of this virulence gene cluster is muc ...
Recombinant DNA Registration Form
Recombinant DNA Registration Form

Know your molecules #2
Know your molecules #2

... Enzyme used to join “sticky ends” of ...
7.1 - DNA Structure
7.1 - DNA Structure

... 7.1.3 - State that nucleosomes help to supercoil chromosomes and help to regulate transcription During supercoiling, the DNA is condensed by a factor of x15000. The histones are responsible for the packaging of DNA at the different levels. The metaphase chromosome is an adaption for mitosis and mei ...
Players in the protein game
Players in the protein game

... • Ribosomes translate the mRNA, written in the language of genes, into amino acids, the language of proteins. They also match nucleic acids with their partner ...
File
File

... a) some may carry oncogenes- genes to send cell cycle out of control b) some viruses may turn on protooncogenes at inappropriate times ...
Ghost in Your Genes Viewing Guide
Ghost in Your Genes Viewing Guide

... NOVA: Ghost in Your Genes (Epigenetics) – Viewing Guide BACKGROUND: "Ghost in Your Genes" focuses on epigenetic "switches" that turn genes "on" or "off." But not all switches are epigenetic; some are genetic. That is, other genes within the chromosome turn genes on or off. In an animal's embryonic s ...
Project Title: Characterization of new genes mediating exchange of
Project Title: Characterization of new genes mediating exchange of

Cloze passage 3
Cloze passage 3

... o) The twisted shape of a DNA molecule p) A biologist who worked with fruit fly to identify sex-linkage q) The features or traits of an organism are controlled by both genes and the ……………. r) The base complementary to thymine s) A model we used to represent chromosomes t) A biological name for a fam ...
Comparative Genome Organization in plants: From Sequence and Markers to... and Chromosomes  Summary
Comparative Genome Organization in plants: From Sequence and Markers to... and Chromosomes Summary

... fails to tell us about chromatin packaging and three-dimensional organization of the chromosomes. Repetitive DNA Sequence and the Large-Scale Organization of the chromosome: Before genomes of different organisms can be compared, the length of the sequence gaps must be determined, the homogeneity of ...
High throughput gene sequencing to identify new genes that cause
High throughput gene sequencing to identify new genes that cause

... myopathies. The life-threatening congenital myopathies are present in all populations, affecting children as well as adults. Considerable progress in human genetics within the past 25 years led to the identification of the molecular basis for 50% of these pathologies. However, the causative mutation ...
Survey: Ethics and Genes
Survey: Ethics and Genes

... Would you want to know about your genetic risk for hundreds of conditions all in one go, ranging from whether you have a higher than average risk from Alzheimer’s disease or diabetes or whether you are sensitive to certain antibiotics or statins? How do you feel about researchers generating this inf ...
Mutations and DNA Technology Notes
Mutations and DNA Technology Notes

... - Making changes to the DNA code of an organism. How can I take a gene from one organism and insert it into another completely different organism? A. Recombinant DNA - DNA made by connecting fragments of DNA from different sources. A + B =C ...
Comparative Genomics Course
Comparative Genomics Course

GBE 214 TECNIQUES IN MOLECULAR BIOLOGY
GBE 214 TECNIQUES IN MOLECULAR BIOLOGY

Supplementary Materials: Immobilization of Genetically
Supplementary Materials: Immobilization of Genetically

... the reaction products were separated by agarose gel electrophoresis, and the insert was purified using  a DNA extraction kit (Omega Bio‐tek).  The monomers were then ligated to the linearized vector (0.4 μL T4 DNA ligase, 2 μL 10× ligation  buffer; 2 μL PUC57‐(VPGXG)20, 15.6 μL insert, incubated at  ...
Drosophila melanogaster
Drosophila melanogaster

file - Athens Academy
file - Athens Academy

... Survey of CentC arrays and CenPC3 in Zea Maize centromeres are mainly composed of two different repetitive sequences: a retrotransposon, CRM, and a 156bp tandem repeat, CentC. In domesticated maize CRM levels are fairly consistent while the amount of CentC varies greatly between inbred lines, land r ...
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Genomic library



A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.
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