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Principle of TAIL-PCR
Principle of TAIL-PCR

Changes in DNA can produce variation
Changes in DNA can produce variation

... • Differences in DNA are what make one organism different from another • The number of differences between two species is large Human 3 billion Base pairs Yeast cell 12 Million Base Pairs ...
Group 4 members
Group 4 members

... – Haploid cell line  powerful global gene disruption; – High throughput deep sequencing  analyze pools of cells, get genome-wide overviews of genes and enable rapid assessment of the spectrum of genes, assigning genes to phenotypes with high saturation and accuracy; ...
BI475 Ch15 SQ
BI475 Ch15 SQ

... transition from the former to latter is thought to have occurred. 3. Which periods during the last 1.5 billion years are linked to sudden increases in gene number? 5. What indications are there that genome duplication has been important during the evolutionary histories of present-day genomes? 6. Us ...
Key for Practice Exam 4
Key for Practice Exam 4

Greatest Discoveries with Bill Nye: Genetics
Greatest Discoveries with Bill Nye: Genetics

... 2. What happened when Mendel crossed a round seed with a wrinkled? 3. What happened when Mendel crossed the round offspring seeds? 4. About how many of the second generation seeds were wrinkled? 5. How many of Mendel’s genetic factors are contributed by each parent? 6. What is a Mendelian trait? Gen ...
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Slide 1

... [email protected] ...
F 1
F 1

... restriction enzymes. Such a map can be used to help locate genes responsible for diseases. 1973 Discovery: First animal gene cloned 1990 Discovery: Launch of the Human Researchers fuse a segment of DNA Genome Project 9 The Department of containing a gene from the African clawed Energy and the Nation ...
Biotechnology and Recombinant DNA
Biotechnology and Recombinant DNA

... • Procedure where cells can take up plasmids (DNA) from the surrounding environment • The cell receiving the new DNA must be ...
HEREDITY: INHERITANCE and TRENDS Unit Cover Page Topic
HEREDITY: INHERITANCE and TRENDS Unit Cover Page Topic

... Each chromosome consists of a single very long DNA molecule, and each gene on the chromosome is a particular segment of the DNA. The instructions for forming species characteristics are carried in DNA. All cells in an organism have the same genetic content, but the genes expressed by the cell may be ...
What are the potential benefits to knowing more - B
What are the potential benefits to knowing more - B

Recombinant DNA and Gene Cloning
Recombinant DNA and Gene Cloning

... Some plasmids are copied at about the same rate as the chromosome, so a single cell is apt to have only a single copy of the plasmid. Other plasmids are copied at a high rate and a single cell may have 50 or more of them. Genes on plasmids with high numbers of copies are usually expressed at high le ...
TRANSCRIPTOMICS
TRANSCRIPTOMICS

... and his colleagues need three months of lectures to convey and his colleagues need three months of lectures to convey  the concept of the gene, and that’s not because the students  are less bright.”   From:  Pearson (2006) Nature, 441: 399 – 401. ...
Human Genome
Human Genome

... much higher in the distal regions (around 20 Mb) of chromosomes and on shorter chromosome arms. In general, in a pattern that promotes the occurrence of at least one crossover per chromosome per arm in each meiosis 11. >1.4 million SNPs have been identified. ...
DNA: The Secret of Life
DNA: The Secret of Life

... Inheritance, but we did not always know this fact. • Inheritance is easy to observe ...
Nic`s genome contains 16124 variants, relative the human reference
Nic`s genome contains 16124 variants, relative the human reference

DNA, Chromosomes & Genes
DNA, Chromosomes & Genes

... • Chromosomes contain a single, long piece of DNA about 4 cm long • A chromosome is about 0.004 mm long • The DNA is about 10 000 times longer than the chromosome – So it has to twist and coil to fit inside ...
What is Bioinformatics I?
What is Bioinformatics I?

... Prerequisites: Graduate standing or consent of instructors. Familiarity with molecular biology or computer methods is not assumed (though a willingness to learn a little about them is required). Topics: (estimated time for discussion) ...
PCR - Polymerase Chain Reaction
PCR - Polymerase Chain Reaction

... – Radiation damage – high energy particles disrupt electronic structure and bonding ...
Glossary 29Sept2012_Genetics
Glossary 29Sept2012_Genetics

... milestones. Once markers have been identified they can be traced back in time to their origin—the most recent common ancestor of everyone who carries the marker. Genetics - The study of the patterns of inheritance of specific traits. genome - The total DNA sequence that serves as an instruction manu ...
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... Subject: The structure and replication of DNA. Reading in ‘An introduction to genetic analysis’ (Griffiths et al., 7th edition) Chapter 8: The structure and replication of DNA. ________________________________________________________________________ Key concepts and keywords: DNA: the genetic materi ...
Repetitive DNA and next-generation sequencing
Repetitive DNA and next-generation sequencing

Unit 8: Inheritance & Human Genetic Patterns
Unit 8: Inheritance & Human Genetic Patterns

... Used to identify genes for specific traits Transfer genes for a specific trait from one organism to another organism. ...
Genetic Engineering / Recombinant DNA technology Genetic
Genetic Engineering / Recombinant DNA technology Genetic

Genetics of prokaryotic organisms
Genetics of prokaryotic organisms

... synthesizes a new strand at the same time. The second strand is also synthesized in the acceptor cell. Then there is recombination between donor and acceptor parts of the chromosome and excision and elimination of incomplete replication. ...
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Genomic library



A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.
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