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... genetic crosses. b. determine the actual outcomes of genetic crosses. c. determine which species should be used in genetic crosses. d. decide which organisms are best to use in genetic crosses. ...
AP Biology Review Sheet for Chapters 18,19, and 20 Test (Test on
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... Understand the different levels of chromatin packing Understand the basic differences between prokaryotic operons and eukaryotic operons Understand the stages of gene expression that can be regulated Understand how cancer causing and suppressing genes operate Understand how gene regulation ties into ...
Comparative genomics
Comparative genomics

... We can set a number of parameters such as: Cost of a gap: how much negative score does a gap in the alignment cause % identity between the query and database Output format: for example a table The most important parameter is possibly the ...
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...  Plasmids are easily manufactured in large amounts  DNA is very stable  DNA resists temperature extremes and so storage and transport are straight forward  A DNA sequence can be changed easily in the laboratory  can respond to changes in the infectious agent  By using the plasmid in the vaccin ...
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... A construct that consists of chloroplast sequences (C and D) that flank two selectable marker genes is inserted into the chloroplast genome through homologous recombination, thereby transforming the native plastome into a TRANSPLASTOME (a). One of the selectable genes (aadA) is designed for exclusiv ...
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... chromosomes containing the same type of genes from each parent. iii. Each of the two daughter cells from meiosis I contain (one, two, or three) chromosome from each parental pair. 14. ______________________________ is the study of patterns of inheritance and variations in organisms. a. (T/F) Genes c ...
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Recombinant DNA technology engineering) involves combining genes from genes.
Recombinant DNA technology engineering) involves combining genes from genes.

... copying a single DNA sequence many times. •A mixture of DNA, DNA polymerase, and nucleotide monomers will continue to replicate, forming a geometrically increasing number of copies. •This technique has revolutionized DNA work because sequences can now be obtained from extremely small ...
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Viruses - CSUN.edu

... Phage injects its DNA into host cell Phage’s nucleic acid merges with the host’s DNA Cell becomes so full that it burst thus releasing Cell divides & phage DNA divides with it a new phage generation that infect more thereby producing more cells with viral DNA bacteria ...
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Genomic library



A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.
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