• Study Resource
  • Explore Categories
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
Document
Document

... virus (ZYMV-NAT) occurs in transgenic plants expressing the plum pox potyvirus (PPV) coat protein (CP) gene. Heteroencapsidation has been shown to be responsible for this modification in the epidemiological characteristics of the infecting virus. In order to prevent this biological risk, several mod ...
PowerPoint file
PowerPoint file

... Genomic Signal Processing • Genomic Signal Processing is a relatively new ...
Karyotypes and Mutations
Karyotypes and Mutations

... • When does crossing over occur? When does independent assortment occur? • Describe the cells that result at the end of meiosis ...
Genetic terms, punnett squares
Genetic terms, punnett squares

... » Plasmids - circular DNA molecules found in bacteria, separate from other bacterial DNA » Sticky ends - matching or complimentary segments of DNA that are produced by restriction enzymes » Human genes can be inserted into bacterial plasmids so the bacteria can produce human enzymes or proteins = re ...
11th Grade Science PPT
11th Grade Science PPT

... A. DNA is composed of only 4 types of nucleotides. B. DNA is composed of smaller subunits than are proteins. C. DNA is abundant in both the nucleus and the cytoplasm. D. The concentration of DNA is generally consistent from cell to cell. ...
Recombinant Paper Plasmids:
Recombinant Paper Plasmids:

... DNA molecules are pieces of DNA that have been reassembled from pieces taken from more than one source of DNA. Often, one of these DNA sources is a plasmid. Plasmids are small, circular DNA molecules that can reside in cells. Plasmids are copied by the cell’s DNA replication enzymes because they con ...
Glencoe Biology
Glencoe Biology

ppt - Department of Computer Science
ppt - Department of Computer Science

... organisms to reproduce ...
GP3 Study Guide - Peoria Public Schools
GP3 Study Guide - Peoria Public Schools

... Base substitution is the simplest type of mutation in which one base in a gene is replaced by another. An example of a disease caused by a base substitution mutation in humans is sickle cell anemia. Natural selection has maintained the sickle cell allele because when it occurs singly, it results in ...
Ch. 14 - The Human Genome
Ch. 14 - The Human Genome

... each other.  DNA fingerprinting makes use of this difference to identify individuals. ...
DNA PPT
DNA PPT

GP3 Study Guide (Topic 3) 2017 Topic 3.1
GP3 Study Guide (Topic 3) 2017 Topic 3.1

... Base substitution is the simplest type of mutation in which one base in a gene is replaced by another. An example of a disease caused by a base substitution mutation in humans is sickle cell anemia. Natural selection has maintained the sickle cell allele because when it occurs singly, it results in ...
Apr. 5 Presentation Mutagenesis Methods
Apr. 5 Presentation Mutagenesis Methods

... Transposon: a piece of short DNA that replicates by inserting into other pieces of DNA (plasmids, chromosomes, etc…) Useful for studying gene function because when the transposon moves into different location in the DNA it may cause a disruption in a gene or a set of genes. Transposons also have man ...
A parasite`s parasite saves host`s neighbours
A parasite`s parasite saves host`s neighbours

... indirectly, by host integration of the virophage DNA sequences, which are expressed only during subsequent encounters with the giant virus. As with CRISPR–Cas-mediated immunity, it is not clear how a host cell survives to retain immunological memory, given that infection by CroV is usually fatal, as ...
ChIP-seq - STAT 115
ChIP-seq - STAT 115

... • Binary decision? • Rank product of • Regulatory potential ...
MCDB 1030 – Spring 2003
MCDB 1030 – Spring 2003

... rigorous way of proving that a particular pathogen caused a particular disease. They are not always valid, though. Viruses and prion cannot be grown in pure culture. Furthermore, many bacteria cannot be grown in pure culture, probably because we don’t understand their growth requirements. Thus, it m ...
File
File

... Process of Using Microarrays to Compare DNA from 2 Different Species • DNA collected from each species • DNA cut into fragments • DNA is denatured to yield single-strand fragments of different lengths • DNA labeled with fluorescent tags ...
rII
rII

... – The gene was viewed as the fundamental unit of change (mutation). – The gene was viewed as the fundamental unit of function (parts of genes were not thought to contain function). ...
pdf
pdf

... The DNA is then cut randomly into about equal pieces (we assume a normal distribution with known mean and variance for each experiment, average sizes are 200bps to 5000bps). These pieces of DNA are then put into a sequencer that can “read” the ends of these pieces (about 100bps). Thus leaving us wit ...
Document
Document

... • Meiosis involves halving the number of chromosomes by division. • Fertilization involves fusing two gametes and restoration of original number of ...
mnw2yr_lec17_2004
mnw2yr_lec17_2004

... Daly et al (2001) were able to infer offspring haplotypes largely from parents. They say that “it became evident that the region could be largely decomposed into discrete haplotype blocks, each with a striking lack of diversity“ The haplotype blocks: – Up to 100kb – 5 or more SNPs For example, this ...
NEW revision booklt - Eduspace
NEW revision booklt - Eduspace

... proteins and held together by another histone protein. ...
11-GeneTech
11-GeneTech

... cut DNA only at specific sequences are called __________________. Often, DNA fragments are cloned by placing them in a ______________ in which the DNA can be replicated within bacteria. Alternatively, the DNA sequence can be replicated entirely in vitro using the __________________ technique. A popu ...
Genetic Engineering
Genetic Engineering

... – It is now possible to insert genes from one organism into another. – Organisms that contain such foreign genes are said to be Transgenic. – Trans- across or moved genes ...
BICH/GENE 431 KNOWLEDGE OBJECTIVES Chapter 9 – Mutations
BICH/GENE 431 KNOWLEDGE OBJECTIVES Chapter 9 – Mutations

... Intercalating agents – know examples; insert between bases in DNA to cause insertions or deletions during replication Direct reversal of damage - DNA photolyase to remove thymine dimers (plants, bacteria, not humans) - Methyltransferase enzyme to repair O6-methylguanine (single turnover) Base excisi ...
< 1 ... 394 395 396 397 398 399 400 401 402 ... 561 >

Genomic library



A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.
  • studyres.com © 2025
  • DMCA
  • Privacy
  • Terms
  • Report