DNA cloning
... produced by cloning DNA fragments from an entire genome • A genomic library that is made using bacteriophages is stored as a collection of phage clones ...
... produced by cloning DNA fragments from an entire genome • A genomic library that is made using bacteriophages is stored as a collection of phage clones ...
Gel Electrophoresis
... Cells are _______ down to __________ DNA – If only a small amount of DNA available, it can be _________ or duplicated using the _____________ ______ __________ (PCR) – ______ - Makes copies of ______ fragments of DNA to work with. ...
... Cells are _______ down to __________ DNA – If only a small amount of DNA available, it can be _________ or duplicated using the _____________ ______ __________ (PCR) – ______ - Makes copies of ______ fragments of DNA to work with. ...
Lecture 19 Evolution of Senescence
... and lifespan in model organisms. A recent study described 3 SNPs in the FOXO3A gene that were statistically significantly associated with longevity in a discovery sample of long-lived men of Japanese ancestry [Willcox et al. (2008) Proc Natl Acad Sci USA 105:13987–13992]. However, this finding requi ...
... and lifespan in model organisms. A recent study described 3 SNPs in the FOXO3A gene that were statistically significantly associated with longevity in a discovery sample of long-lived men of Japanese ancestry [Willcox et al. (2008) Proc Natl Acad Sci USA 105:13987–13992]. However, this finding requi ...
Leishmania major Friedlin chromosome 1 has an unusual
... Communicated by Seymour J. Klebanoff, University of Washington School of Medicine, Seattle, WA, December 28, 1998 (received for review December 2, 1998) ...
... Communicated by Seymour J. Klebanoff, University of Washington School of Medicine, Seattle, WA, December 28, 1998 (received for review December 2, 1998) ...
Chap3 Recombinant DNA
... Multiple cloning sites: allow the choice of different restriction enzyme (containing many restriction recognition sites) ...
... Multiple cloning sites: allow the choice of different restriction enzyme (containing many restriction recognition sites) ...
REVIEW 5: GENETICS 1. Chromosomes
... _ than the X, so it is missing many genes. This means many genes on the X chromosome do not have a “partner”. ...
... _ than the X, so it is missing many genes. This means many genes on the X chromosome do not have a “partner”. ...
Point mutation - Chavis Biology
... eliminating the cause of the disorder. However, gene therapy has had limited success because the hose often rejects the injected genetic material. ...
... eliminating the cause of the disorder. However, gene therapy has had limited success because the hose often rejects the injected genetic material. ...
Basic Concepts of Human Genetics
... responsible for controlling the human genetic behavior. The coding region is called extron. ⎯ There are totally about 40,000 genes, over 5000 have been identified. There are much more left ⎯ Human Genome Project is to identified the DNA sequence (every bp) of human genome ( only a few individuals) ⎯ ...
... responsible for controlling the human genetic behavior. The coding region is called extron. ⎯ There are totally about 40,000 genes, over 5000 have been identified. There are much more left ⎯ Human Genome Project is to identified the DNA sequence (every bp) of human genome ( only a few individuals) ⎯ ...
Biology 303 EXAM II 3/14/00 NAME
... One bacterial Hfr strain transfers genes in the order A--B--C--D--> while a second Hfr strain transfers genes in the order B--C--D--A-->. The most likely explanation for this is that 1. one strain actually carries an F' element and is a merozygote. 2. the F factor integrated at the same site but in ...
... One bacterial Hfr strain transfers genes in the order A--B--C--D--> while a second Hfr strain transfers genes in the order B--C--D--A-->. The most likely explanation for this is that 1. one strain actually carries an F' element and is a merozygote. 2. the F factor integrated at the same site but in ...
Biology 303 EXAM II 3/14/00 NAME
... Sample Questions to help you prepare for Exam II, which is scheduled for October 20, 2005. Your real exam will consist of 40 multiple choice questions. Answers are boldface and underlined. If you have questions, please let me know. ...
... Sample Questions to help you prepare for Exam II, which is scheduled for October 20, 2005. Your real exam will consist of 40 multiple choice questions. Answers are boldface and underlined. If you have questions, please let me know. ...
RNA Seq: A (soon to be outdated) Tutorial
... K (the actual number of balls) follows a Poisson distribution and hence K varies around the expected value (u) with a standard deviation of 1/ sqroot (u) Microarray data follows a Poisson distribution. However RNA seq does not. In RNA Seq genes with high mean counts (either because they’re long or h ...
... K (the actual number of balls) follows a Poisson distribution and hence K varies around the expected value (u) with a standard deviation of 1/ sqroot (u) Microarray data follows a Poisson distribution. However RNA seq does not. In RNA Seq genes with high mean counts (either because they’re long or h ...
Genetic Manipulation of Bacteria
... random sequencing depends on high copy number vectors carrying small fragments, ...
... random sequencing depends on high copy number vectors carrying small fragments, ...
Whose DNA was sequenced for the Human Genome Project?
... Now that we have a map of the human genome, we have to learn how to read it. That means figuring out which gene does what. Of the estimated 30,000 genes in the human genome, we have very little idea about what each one does. One way of studying genes is to directly compare the entire genome with ot ...
... Now that we have a map of the human genome, we have to learn how to read it. That means figuring out which gene does what. Of the estimated 30,000 genes in the human genome, we have very little idea about what each one does. One way of studying genes is to directly compare the entire genome with ot ...
DNA
... the part that entered the cell was DNA or protein They grew viral cultures in substrate containing radioactive phosphate isotopes (phosphate is found in DNA but not protein) Radioactive DNA They let the virus infect a cell Tests showed that the bacteria became radioactive ...
... the part that entered the cell was DNA or protein They grew viral cultures in substrate containing radioactive phosphate isotopes (phosphate is found in DNA but not protein) Radioactive DNA They let the virus infect a cell Tests showed that the bacteria became radioactive ...
Presented By: Chantille Haynes, Hilary Price, and Richard Dalton
... - Frequency of resistant cultures depends on the amount of transforming (resistant) DNA added. - DNA is inserted via double crosover. Transfomation can also be used to asses the tightness of gene linkages. - DNA extraction for transformation causes breakage of the DNA. - The closer two genes are, th ...
... - Frequency of resistant cultures depends on the amount of transforming (resistant) DNA added. - DNA is inserted via double crosover. Transfomation can also be used to asses the tightness of gene linkages. - DNA extraction for transformation causes breakage of the DNA. - The closer two genes are, th ...
nature v. nurture
... "This is how the environment talks to the genome," said Rudolf Jaenisch, a geneticist at the Whitehead Institute for Biomedical Research in Cambridge, Mass., who was not part of the project. "This paper says lifestyle, or environmental influences or whatever you want to call them, have a real influe ...
... "This is how the environment talks to the genome," said Rudolf Jaenisch, a geneticist at the Whitehead Institute for Biomedical Research in Cambridge, Mass., who was not part of the project. "This paper says lifestyle, or environmental influences or whatever you want to call them, have a real influe ...
DNA
... Crossing over • Two homologous chromosomes exchange genetic information. This increases genetic diversity. ...
... Crossing over • Two homologous chromosomes exchange genetic information. This increases genetic diversity. ...
Slide 1
... DNA, perhaps it would be easier to make RNA than DNA. Could life be based on RNA? What other molecules are possible? ...
... DNA, perhaps it would be easier to make RNA than DNA. Could life be based on RNA? What other molecules are possible? ...
DNA Review
... • More alleles the marker has – more variation is possible between any two people ...
... • More alleles the marker has – more variation is possible between any two people ...
File
... iii. example – glowing mice = the gene responsible for phosphorescence in jellyfish is inserted into mouse embryo’s causing them to glow under an ultraviolet light 1. plasmids - small circles of DNA in bacterium 2. host cell – bacterium or yeast 3. restriction enzymes- (endonucleases) finds and reco ...
... iii. example – glowing mice = the gene responsible for phosphorescence in jellyfish is inserted into mouse embryo’s causing them to glow under an ultraviolet light 1. plasmids - small circles of DNA in bacterium 2. host cell – bacterium or yeast 3. restriction enzymes- (endonucleases) finds and reco ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.