LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
... 08. The opine synthesized by Nop. Ti plasmid is acetosyringone. 09. pBR 322 is constructed from pUC. 10. Sodium alginate is used as fusogent. III. Complete the following 11. Hot air oven is used for …………. of glassware. 12. PEG refers to ………… 13. Agrobacterium tumefasciens causes a disease called ……… ...
... 08. The opine synthesized by Nop. Ti plasmid is acetosyringone. 09. pBR 322 is constructed from pUC. 10. Sodium alginate is used as fusogent. III. Complete the following 11. Hot air oven is used for …………. of glassware. 12. PEG refers to ………… 13. Agrobacterium tumefasciens causes a disease called ……… ...
Particle bombardment
... Electroporated pollen can supposedly germinate at 30% efficiency. However, no transgenic plant has so far been reported using this concept, even though it has been shown that pollen grains can be permeated with macromolecules such as DNA. Electroporation method is very efficient in permeating DNA in ...
... Electroporated pollen can supposedly germinate at 30% efficiency. However, no transgenic plant has so far been reported using this concept, even though it has been shown that pollen grains can be permeated with macromolecules such as DNA. Electroporation method is very efficient in permeating DNA in ...
MGG330 L1-2007
... Probe sets are designed to 3’ end of gene as labelling of probe starts at “end” of gene ...
... Probe sets are designed to 3’ end of gene as labelling of probe starts at “end” of gene ...
Simple and straightforward construction of a mouse gene targeting
... are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permission ...
... are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permission ...
DNA SEQUENCING DNA sequencing
... More importantly, these methods do not require PCR, which creates mutations in clonally amplified templates that masquerade as sequence variants. AT-rich and GC-rich target sequences may also show amplification bias in product yield, which results in their under representation in genome alignments a ...
... More importantly, these methods do not require PCR, which creates mutations in clonally amplified templates that masquerade as sequence variants. AT-rich and GC-rich target sequences may also show amplification bias in product yield, which results in their under representation in genome alignments a ...
Reading, pages 46-55 HEADING: “From Mendel to the Human
... Draw a Punnett Square for the offspring of parent pea plants that each have the genotype “Tt” for the feature of height. Draw it here What fraction of these offspring would be short? ________________ ...
... Draw a Punnett Square for the offspring of parent pea plants that each have the genotype “Tt” for the feature of height. Draw it here What fraction of these offspring would be short? ________________ ...
Note 8.1 - Cloning DNA
... Host Cell – is a cell that has taken up a foreign plasmid or virus and has used its cellular machinery to express the foreign DNA. Cloned Gene – is an identical copy of an original target gene that can be made by introducing the target gene into a host cell and having it copied. Plasmids are require ...
... Host Cell – is a cell that has taken up a foreign plasmid or virus and has used its cellular machinery to express the foreign DNA. Cloned Gene – is an identical copy of an original target gene that can be made by introducing the target gene into a host cell and having it copied. Plasmids are require ...
Practice Questions 1: Genetics
... cell membranes, proteins, and enzymes during environmental stress. The resulting plants survive drought, low temperatures, salty soils, and other stresses better than standard rice varieties. Which substance from bacteria was most likely inserted into rice plants in the development of the ...
... cell membranes, proteins, and enzymes during environmental stress. The resulting plants survive drought, low temperatures, salty soils, and other stresses better than standard rice varieties. Which substance from bacteria was most likely inserted into rice plants in the development of the ...
What do Genes Look Like - Effingham County Schools
... Ex: German Shepard x German Shepard = German Shepard VII. _______________________________ – Desired genes are removed from one organism and added or recombined into another organism. This forms a transgenic organism with recombinant DNA A. This is used to make proteins not normally made by the cel ...
... Ex: German Shepard x German Shepard = German Shepard VII. _______________________________ – Desired genes are removed from one organism and added or recombined into another organism. This forms a transgenic organism with recombinant DNA A. This is used to make proteins not normally made by the cel ...
Chapter 20 DNA Technology
... differences in DNA code result in different restriction sites in DNA produces fragments of different lengths = restriction fragment length polymorphisms (RFLP's) treat DNA with restriction enzymes use gel electrophoresis to separate the restriction fragments AGAROSE GEL ELECTROPHORESIS = “sw ...
... differences in DNA code result in different restriction sites in DNA produces fragments of different lengths = restriction fragment length polymorphisms (RFLP's) treat DNA with restriction enzymes use gel electrophoresis to separate the restriction fragments AGAROSE GEL ELECTROPHORESIS = “sw ...
File
... • If a male gamete and a female gamete both had regular amounts of DNA, then when they combined there would be twice as much as was needed. • To solve this problem gamete cells undergo a process called meiosis. • Meiosis is a type of cell division where each cell ends up with only half the DNA of a ...
... • If a male gamete and a female gamete both had regular amounts of DNA, then when they combined there would be twice as much as was needed. • To solve this problem gamete cells undergo a process called meiosis. • Meiosis is a type of cell division where each cell ends up with only half the DNA of a ...
DNA Structure and Replication
... DNA chains can be very long ! E. coli chromosome: 4.6x106 base pairs: 4.6x 106 x .34 nm = 1.5x106 nm = 1.5 mm ! Human DNA: 6x10-12 g/cell x 1/660 mol bp/g x 6.023x1023 bp/mol bp x 0.34x10-9 m/bp = 1.9 m ! Bacterial, viral DNA “chromosomes” are circles ! DNA in human chromosomes (and DNA of all euka ...
... DNA chains can be very long ! E. coli chromosome: 4.6x106 base pairs: 4.6x 106 x .34 nm = 1.5x106 nm = 1.5 mm ! Human DNA: 6x10-12 g/cell x 1/660 mol bp/g x 6.023x1023 bp/mol bp x 0.34x10-9 m/bp = 1.9 m ! Bacterial, viral DNA “chromosomes” are circles ! DNA in human chromosomes (and DNA of all euka ...
chapt09_lecture
... carrier of DNA from a donor cell to a recipient cell • Two types: – Generalized transduction – random fragments of disintegrating host DNA are picked up by the phage during assembly; any gene can be transmitted this way – Specialized transduction – a highly specific part of the host genome is regula ...
... carrier of DNA from a donor cell to a recipient cell • Two types: – Generalized transduction – random fragments of disintegrating host DNA are picked up by the phage during assembly; any gene can be transmitted this way – Specialized transduction – a highly specific part of the host genome is regula ...
Recombinant Adenovirus In Molecular Biology
... infect more flasks of HEKs • Then more adenovirus collected, more HEKs infected. • Repeat until titer is high, than purify. ...
... infect more flasks of HEKs • Then more adenovirus collected, more HEKs infected. • Repeat until titer is high, than purify. ...
No Slide Title
... 21. An ultracentrifuge consists of a rotor that spins tubes containing materials and is: (A) a component on a new type of microscope to allow cell components to be easily visualized (B) the laboratory tool developed by Robert Hooke in the 1660s that he used to discover cells (C) a tool used by cell ...
... 21. An ultracentrifuge consists of a rotor that spins tubes containing materials and is: (A) a component on a new type of microscope to allow cell components to be easily visualized (B) the laboratory tool developed by Robert Hooke in the 1660s that he used to discover cells (C) a tool used by cell ...
39 Karyotyping and Chromosomes Discovering
... treatments for humans (most human insulin is made by bacteria today). In the past, we succeeded in breeding certain traits into animals (larger cattle, cows that give more milk) by selective breeding. Selective breeding can be when you specifically mate a particular animal that has certain desirable ...
... treatments for humans (most human insulin is made by bacteria today). In the past, we succeeded in breeding certain traits into animals (larger cattle, cows that give more milk) by selective breeding. Selective breeding can be when you specifically mate a particular animal that has certain desirable ...
Document
... Recombinant DNA technology 1. identify gene you want to transfer to target organism. 2. use restriction enzymes to remove the gene from the host organism. 3. use the same restriction enzymes to cut a plasmid. Now both the plasmid and the genes have the same overhangs--they can fit together like puzz ...
... Recombinant DNA technology 1. identify gene you want to transfer to target organism. 2. use restriction enzymes to remove the gene from the host organism. 3. use the same restriction enzymes to cut a plasmid. Now both the plasmid and the genes have the same overhangs--they can fit together like puzz ...
ppt - Barley World
... “The Helios Gene Gun is a new way for in vivo transformation of cells or organisms apy and genetic immunization (DNA vaccination)). This gun uses Biolistic ® particle bombardment where DNA- or RNA-coated gold particles are loaded into the gun and you pull the trigger. A low pressure helium pulse del ...
... “The Helios Gene Gun is a new way for in vivo transformation of cells or organisms apy and genetic immunization (DNA vaccination)). This gun uses Biolistic ® particle bombardment where DNA- or RNA-coated gold particles are loaded into the gun and you pull the trigger. A low pressure helium pulse del ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.