Isolation of a Transforming Sequence from a Human Bladder
... the Cot = 1 probe were pooled together (data not shown). This enriched-fragment mixture was kinked to the genomic ends of the Charon 4A bacteriophage vector and packaged in vitro (Blattner et al., 1977). The resulting chimeric phage stock contained an estimated 5 x 1 O5 plaque-forming units. These w ...
... the Cot = 1 probe were pooled together (data not shown). This enriched-fragment mixture was kinked to the genomic ends of the Charon 4A bacteriophage vector and packaged in vitro (Blattner et al., 1977). The resulting chimeric phage stock contained an estimated 5 x 1 O5 plaque-forming units. These w ...
Gel Electrophoresis
... • Lithium borate (LB) is relatively new but it has low conductivity, so much higher voltage could be used, which means a shorter analysis time for routine electrophoresis. ...
... • Lithium borate (LB) is relatively new but it has low conductivity, so much higher voltage could be used, which means a shorter analysis time for routine electrophoresis. ...
Document
... benefitted if animals are developed that can withstand hypoxic stress. It would also be important to know if this gene has any relationship with growth, to determine whether normal growth is affected during prolonged periods of hypoxia. HIF-1α has been widely studied in various mammalian and fish sp ...
... benefitted if animals are developed that can withstand hypoxic stress. It would also be important to know if this gene has any relationship with growth, to determine whether normal growth is affected during prolonged periods of hypoxia. HIF-1α has been widely studied in various mammalian and fish sp ...
Document
... machines and the dideoxy chain termination method A major thrust of the project was development of technology for faster sequencing ...
... machines and the dideoxy chain termination method A major thrust of the project was development of technology for faster sequencing ...
File
... machines and the dideoxy chain termination method A major thrust of the project was development of technology for faster sequencing ...
... machines and the dideoxy chain termination method A major thrust of the project was development of technology for faster sequencing ...
Ribosome Display: In Vitro Selection of Protein
... allowing the selection from very large combinatorial libraries. In add ition, the ropid selection cycles require an integra l polymerase cboin re«ction (PCR) step, which can be used for ra ndomization, making this method ideal for directed evolution experiments. The fact that the ribosomal complex u ...
... allowing the selection from very large combinatorial libraries. In add ition, the ropid selection cycles require an integra l polymerase cboin re«ction (PCR) step, which can be used for ra ndomization, making this method ideal for directed evolution experiments. The fact that the ribosomal complex u ...
ch_07_clicker_questions
... c) Chromosomes float freely in the cytosol. d) Eukaryotic cells contain no genetic material outside of their chromosomes. ...
... c) Chromosomes float freely in the cytosol. d) Eukaryotic cells contain no genetic material outside of their chromosomes. ...
DNA Lesson 2 Guide
... Cue students about building: “Listen to these specific building instructions: To make the gene correctly, you should place each nucleotide on top of its picture. Double check your work, too! Remember to be careful with the arrows. Any questions? You may begin.” ☞☞ Hand out gene strips. Make sure to ...
... Cue students about building: “Listen to these specific building instructions: To make the gene correctly, you should place each nucleotide on top of its picture. Double check your work, too! Remember to be careful with the arrows. Any questions? You may begin.” ☞☞ Hand out gene strips. Make sure to ...
Name
... Together, each pair of chromosomes is referred to as a homologous pair. In this context, homologous means “having the same structure.” Homologous chromosomes are two chromosomes—one inherited from the mother, one from the father—that have the same length and general appearance. More importantly, the ...
... Together, each pair of chromosomes is referred to as a homologous pair. In this context, homologous means “having the same structure.” Homologous chromosomes are two chromosomes—one inherited from the mother, one from the father—that have the same length and general appearance. More importantly, the ...
Biocatalytic degradation of pollutants
... proteins. These reports are of interest, because they indicate that these bacteria have not only evolved new pathways for the degradation of man-made chemicals, but that they have also evolved appropriate chemosensory systems for their detection. In another study, three Pseudomonas strains were foun ...
... proteins. These reports are of interest, because they indicate that these bacteria have not only evolved new pathways for the degradation of man-made chemicals, but that they have also evolved appropriate chemosensory systems for their detection. In another study, three Pseudomonas strains were foun ...
Recent progress on the Ada response for inducible repair of DNA
... direct acting alkylating agents may be formed by nitrosations, in slightly acidic conditions, of amides, amines, amino acids and peptides (Harrison et al., 1999; Sedgwick, 1997; Sedgwick and Vaughan, 1991). These reactions could possibly occur in decaying matter, in acidic soils or in putrid water. ...
... direct acting alkylating agents may be formed by nitrosations, in slightly acidic conditions, of amides, amines, amino acids and peptides (Harrison et al., 1999; Sedgwick, 1997; Sedgwick and Vaughan, 1991). These reactions could possibly occur in decaying matter, in acidic soils or in putrid water. ...
A novel gene encoding a 54 kDa polypeptide is
... rapidly and unambiguously from environmental samples. Since methane could also be of recent geological origin, methane-utilizing bacteria were not considered in our investigation. The conventional method for detection of specific microorganisms is selective plating. Although easy to use, this method ...
... rapidly and unambiguously from environmental samples. Since methane could also be of recent geological origin, methane-utilizing bacteria were not considered in our investigation. The conventional method for detection of specific microorganisms is selective plating. Although easy to use, this method ...
(S) tet Resistance Determinant Element Containing the Tetracycline
... the latter explanation, as the 4.8-kb hybridizing HindIII fragment with the int/xis probe is the same size as one of the fragments when tet(S) is used as the probe, indicating that the tet(S) gene and one of the int/xis regions are linked. When pAM120 is used to probe the blots, HincII digestion sho ...
... the latter explanation, as the 4.8-kb hybridizing HindIII fragment with the int/xis probe is the same size as one of the fragments when tet(S) is used as the probe, indicating that the tet(S) gene and one of the int/xis regions are linked. When pAM120 is used to probe the blots, HincII digestion sho ...
Molecular Cytogenetics
... Lymphoblastoid cells were immortalized by standard techniques. Genomic DNA was purified as described above and submitted to Nimblegen Systems Inc. (Madison, WI) for whole genome array CGH using an array containing ~385,000 probes (Cat. No. B4366-00-01), with pooled normal human male reference DNA (P ...
... Lymphoblastoid cells were immortalized by standard techniques. Genomic DNA was purified as described above and submitted to Nimblegen Systems Inc. (Madison, WI) for whole genome array CGH using an array containing ~385,000 probes (Cat. No. B4366-00-01), with pooled normal human male reference DNA (P ...
Pandora - W.W. Norton
... arguments are fast becoming relics of the past. As an example of the anachronistic nature of debates over canon reformation, notions of the canon prevalent in the twentieth century fail to account for changes in technology and literature. The rise of the Internet, for instance, has changed how peopl ...
... arguments are fast becoming relics of the past. As an example of the anachronistic nature of debates over canon reformation, notions of the canon prevalent in the twentieth century fail to account for changes in technology and literature. The rise of the Internet, for instance, has changed how peopl ...
White biotechnology report RIVM
... available technique at the moment. It is mainly used for eukaryotic cells (fungi, yeasts), but prokaryotic organisms (bacteria, archaea) will probably follow. Still, the interviewed experts from industry were hesitant about the full‐fledged use of the technique in industrial microbi‐ ology, also b ...
... available technique at the moment. It is mainly used for eukaryotic cells (fungi, yeasts), but prokaryotic organisms (bacteria, archaea) will probably follow. Still, the interviewed experts from industry were hesitant about the full‐fledged use of the technique in industrial microbi‐ ology, also b ...
Development of novel computational tools based on
... The transfer of operational genes is a continual process and is far more important in prokaryotic diversity of different sources (Jain et al., 1999; Ochman, 2001). For horizontal gene transfer to become a success, the acquisition of foreign DNA segments must be counterbalanced by DNA loss (Lawrence, ...
... The transfer of operational genes is a continual process and is far more important in prokaryotic diversity of different sources (Jain et al., 1999; Ochman, 2001). For horizontal gene transfer to become a success, the acquisition of foreign DNA segments must be counterbalanced by DNA loss (Lawrence, ...
Ribosomal RNA Genes Investigation Part I: Gene Copy Number The
... 20th Century molecular biology was recognizing the essential role of rRNAs, and the Harry Noller Lab at UCSC made sustained and essential contributions to this field of research. Let's see if we can use some basic bioinformatics tools to learn more about the functional organization and evolution of ...
... 20th Century molecular biology was recognizing the essential role of rRNAs, and the Harry Noller Lab at UCSC made sustained and essential contributions to this field of research. Let's see if we can use some basic bioinformatics tools to learn more about the functional organization and evolution of ...
DNA: The Genetic Material
... part of the of the genetic sequence in that cell and in future daughter cells. The cell may die or simply not perform its normal function. These mutations are not passed on to the next generation. When mutations occur in sex cells, they will be present in every cell of the offspring. ...
... part of the of the genetic sequence in that cell and in future daughter cells. The cell may die or simply not perform its normal function. These mutations are not passed on to the next generation. When mutations occur in sex cells, they will be present in every cell of the offspring. ...
Overview of Conjugation
... chromosome. • Allows very high frequency transfer of F’ markers. • Allows one to make partial diploids. • Can use for complementation tests. ...
... chromosome. • Allows very high frequency transfer of F’ markers. • Allows one to make partial diploids. • Can use for complementation tests. ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.