Transposition - Pennsylvania State University
... Transposable elements • Mobile genetic elements - they move from one location in the genome to another • Found in all organisms (so far studied) • Effects: – Insertion near or within a gene can inactivate or activate the target gene. – Cause deletions, inversions, and translocations of DNA – Lead t ...
... Transposable elements • Mobile genetic elements - they move from one location in the genome to another • Found in all organisms (so far studied) • Effects: – Insertion near or within a gene can inactivate or activate the target gene. – Cause deletions, inversions, and translocations of DNA – Lead t ...
guidelines
... - the same day you did not enter any other labs yet; - UV lights are already turned off; - ventillation (for higher pressure) is switched on; - you have everything with you which will be needed for your work to avoid unnecessary traffic from and to the lab. - bring the minimum amount of museum/biolo ...
... - the same day you did not enter any other labs yet; - UV lights are already turned off; - ventillation (for higher pressure) is switched on; - you have everything with you which will be needed for your work to avoid unnecessary traffic from and to the lab. - bring the minimum amount of museum/biolo ...
GeneCensus - Gerstein Lab Publications
... Both of these views are linked to additional modules representing more traditional analysis formats. These include modules that examine open reading frames (ORFs), organisms, and various compositions of genomes. In general, it is relatively difficult to integrate disparate information sources into o ...
... Both of these views are linked to additional modules representing more traditional analysis formats. These include modules that examine open reading frames (ORFs), organisms, and various compositions of genomes. In general, it is relatively difficult to integrate disparate information sources into o ...
Recombinases
... at the binding step of the reaction (53). Several clever approaches have recently been used to select Flp and Cre variants with relaxed and/or altered specificity (49–51). ...
... at the binding step of the reaction (53). Several clever approaches have recently been used to select Flp and Cre variants with relaxed and/or altered specificity (49–51). ...
Probing b-Lactamase Structure and Function Using Random Replacement Mutagenesis.
... those for the wild-type enzyme. We also found a few exceptional regions where only a few random sequences function. Examination of the Xray structures of homologous p-lactamases indicates that the regions most sensitive to substitution are in the vicinity of the active site pocket or buried in the h ...
... those for the wild-type enzyme. We also found a few exceptional regions where only a few random sequences function. Examination of the Xray structures of homologous p-lactamases indicates that the regions most sensitive to substitution are in the vicinity of the active site pocket or buried in the h ...
pSAT vectors: a modular series of plasmids for autofluorescent
... sites of pSAT6-EGFP-C1, replacing the 35S promoter and TL sequences and producing pSAT6NP-DEST-EGFP-C1. Plasmids were maintained in E. coli DB3.1 strain due to toxicity of the ccdB gene contained within the conversion cassette in most other bacterial strains. Complete DNA sequences of pSAT6-DEST-EGF ...
... sites of pSAT6-EGFP-C1, replacing the 35S promoter and TL sequences and producing pSAT6NP-DEST-EGFP-C1. Plasmids were maintained in E. coli DB3.1 strain due to toxicity of the ccdB gene contained within the conversion cassette in most other bacterial strains. Complete DNA sequences of pSAT6-DEST-EGF ...
1. If the inside ends
... 1. Replicative transposase cuts only one strand at the junction. 2. Nonreplicative transposase makes cuts in both strands in the junction. • The similarity between replicative and nonreplicative transposition 1. The cut 5’ ends of the target DNA are joined to the free 3’ ends of the transposon. 2. T ...
... 1. Replicative transposase cuts only one strand at the junction. 2. Nonreplicative transposase makes cuts in both strands in the junction. • The similarity between replicative and nonreplicative transposition 1. The cut 5’ ends of the target DNA are joined to the free 3’ ends of the transposon. 2. T ...
Milestone3
... expected GC content of 38% (as context, chromosome #7 in the yeast genome contains 1,090,946 nucleotides). How many ORFs are there in your randomly generated sequence? How many ORFs in your randomly generated sequence are at least 100nt in length but no more than 2000nt in length? How many ORFs have ...
... expected GC content of 38% (as context, chromosome #7 in the yeast genome contains 1,090,946 nucleotides). How many ORFs are there in your randomly generated sequence? How many ORFs in your randomly generated sequence are at least 100nt in length but no more than 2000nt in length? How many ORFs have ...
overview - El Paso High School
... DNA replication begins with the binding of a large protein complex—the pre-replication complex—to a specific site on the DNA molecule. The complex contains DNA polymerase, which catalyzes addition of nucleotides. The complex binds to a region on the chromosome called the origin of replication (ori). ...
... DNA replication begins with the binding of a large protein complex—the pre-replication complex—to a specific site on the DNA molecule. The complex contains DNA polymerase, which catalyzes addition of nucleotides. The complex binds to a region on the chromosome called the origin of replication (ori). ...
video slide - Course
... Because only cells that can make both arginine and tryptophan (arg+ trp+ cells) can grow into colonies on minimal medium, the lack of colonies on the two control plates showed that no further mutations had occurred restoring this ability to cells of the mutant strains. Thus, each cell from the mixtu ...
... Because only cells that can make both arginine and tryptophan (arg+ trp+ cells) can grow into colonies on minimal medium, the lack of colonies on the two control plates showed that no further mutations had occurred restoring this ability to cells of the mutant strains. Thus, each cell from the mixtu ...
File
... Because only cells that can make both arginine and tryptophan (arg+ trp+ cells) can grow into colonies on minimal medium, the lack of colonies on the two control plates showed that no further mutations had occurred restoring this ability to cells of the mutant strains. Thus, each cell from the mixtu ...
... Because only cells that can make both arginine and tryptophan (arg+ trp+ cells) can grow into colonies on minimal medium, the lack of colonies on the two control plates showed that no further mutations had occurred restoring this ability to cells of the mutant strains. Thus, each cell from the mixtu ...
video slide - Biology Junction
... Because only cells that can make both arginine and tryptophan (arg+ trp+ cells) can grow into colonies on minimal medium, the lack of colonies on the two control plates showed that no further mutations had occurred restoring this ability to cells of the mutant strains. Thus, each cell from the mixtu ...
... Because only cells that can make both arginine and tryptophan (arg+ trp+ cells) can grow into colonies on minimal medium, the lack of colonies on the two control plates showed that no further mutations had occurred restoring this ability to cells of the mutant strains. Thus, each cell from the mixtu ...
Binding of the EcoRII methyltransferase to 5
... Mbol and Main sites. To prove that the original polymer was a repeating polymer containing this sequence it was digested with each of these restriction enzymes. The digests each yielded one fragment having identical mobilities on electrophoresis (figure 1). The basis for the synthesis of this polyme ...
... Mbol and Main sites. To prove that the original polymer was a repeating polymer containing this sequence it was digested with each of these restriction enzymes. The digests each yielded one fragment having identical mobilities on electrophoresis (figure 1). The basis for the synthesis of this polyme ...
Chapter 10b 2012 File
... • There is no DNA replication beforehand • Starts with 2 - 2n cells; after separating centromes (chromatids) results in 4 – 1n cells ...
... • There is no DNA replication beforehand • Starts with 2 - 2n cells; after separating centromes (chromatids) results in 4 – 1n cells ...
DNA and Gene Expression (chaps 12-15)
... 77 1. Describe the biochemical composition, structure, and replication of DNA. Discuss what drives the adding on nucleotides. 2. Describe the steps of protein synthesis, beginning with the attachment of a messenger RNA molecule to the small subunit of a ribosome and ending generalized with the relea ...
... 77 1. Describe the biochemical composition, structure, and replication of DNA. Discuss what drives the adding on nucleotides. 2. Describe the steps of protein synthesis, beginning with the attachment of a messenger RNA molecule to the small subunit of a ribosome and ending generalized with the relea ...
Amino Acids of the Sulfolobus solfataricus Mini-chromosome
... Plasmids—The E. coli expression vector pET19b-SsoMCM was described previously (22). SsoMCM was mutated at lysines 129, 134, and 194 and at histidine 146 to alanine by PCR-based mutagenesis of the corresponding gene (30). The synthetic oligonucleotides used to create the site-directed mutant proteins ...
... Plasmids—The E. coli expression vector pET19b-SsoMCM was described previously (22). SsoMCM was mutated at lysines 129, 134, and 194 and at histidine 146 to alanine by PCR-based mutagenesis of the corresponding gene (30). The synthetic oligonucleotides used to create the site-directed mutant proteins ...
Additional file 4 - Springer Static Content Server
... translocation was identified from circulating fetal DNA in maternal plasma.[17] At the same time, extensive genomewide microarrays and focused analysis would have to be used for this currently reported application. Genomewide high density microarrays can be used immediately to follow up the apparent ...
... translocation was identified from circulating fetal DNA in maternal plasma.[17] At the same time, extensive genomewide microarrays and focused analysis would have to be used for this currently reported application. Genomewide high density microarrays can be used immediately to follow up the apparent ...
Risk assessment of T-DNA borders from Agrobacterium tumefaciens
... However, we performed this at the amino acid level. We translated the whole genome sequence of the plant into amino acid sequences, using three reading frames, and two directions, leading to six amino acid sequences per whole genome. Then we aligned the translated remainder of the left border (Table ...
... However, we performed this at the amino acid level. We translated the whole genome sequence of the plant into amino acid sequences, using three reading frames, and two directions, leading to six amino acid sequences per whole genome. Then we aligned the translated remainder of the left border (Table ...
Origin, genetic diversity, and genome structure of the domestic dog
... dogs have protein alleles in common with wolves,(11,12) share highly polymorphic microsatellites,(13) and have mitochondrial DNA sequences similar or identical to those found in gray wolves.(14,15) Recently, a comprehensive survey of several hundred gray wolves and dogs found that the two species ha ...
... dogs have protein alleles in common with wolves,(11,12) share highly polymorphic microsatellites,(13) and have mitochondrial DNA sequences similar or identical to those found in gray wolves.(14,15) Recently, a comprehensive survey of several hundred gray wolves and dogs found that the two species ha ...
to 3 - NUAMESAPBio
... ▪ To work directly with specific genes, scientists prepare well-defined segments of DNA in identical copies, a process called DNA cloning ▪ Most methods for cloning pieces of DNA in the laboratory share general features ...
... ▪ To work directly with specific genes, scientists prepare well-defined segments of DNA in identical copies, a process called DNA cloning ▪ Most methods for cloning pieces of DNA in the laboratory share general features ...
Evolutionary Genomics of Fast Evolving Tunicates
... Kowalevsky, first recognized that tunicates (also known as urochordates) have vertebrate-like characteristics and postulated that both vertebrates and cephalochordate may have evolved from a tunicate-like ancestor during or prior to the Cambrian explosion. The significance of this discovery was imme ...
... Kowalevsky, first recognized that tunicates (also known as urochordates) have vertebrate-like characteristics and postulated that both vertebrates and cephalochordate may have evolved from a tunicate-like ancestor during or prior to the Cambrian explosion. The significance of this discovery was imme ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.