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publication
publication

... provide further refinement to the human gene map previously described (11). A minimal tiling path determined by hybridization, PCR and in silico methods clearly establishes MUC3 on the telomeric side of ACHE and links the HRBL end of AF053356 to the CYP3A4 locus (see http://www.genet.sickkids.on.ca/ ...
Of dups and dinos:
Of dups and dinos:

... Indeed, during the last 150–200 million years of plant evolution, some lineages have experienced maybe four to five WGDs, but most no more than one or two (Figure 1). On the other hand, tens of thousands of now-living species, both plants and animals, are polyploid, and contain multiple copies of th ...
What traits are carried on mobile
What traits are carried on mobile

... significance of horizontally transferred genes remains to be fully addressed. There are increasing hints of important separations of functions between the vertically transmitted core genome, which encodes fundamental cellular processes, and the horizontally transmissible accessory genome, which enco ...
Sequences of flavivirus-related RNA viruses persist in DNA form
Sequences of flavivirus-related RNA viruses persist in DNA form

Analysis of Toxoplasma gondii Repeat Region 529 bp (NCBI Acc
Analysis of Toxoplasma gondii Repeat Region 529 bp (NCBI Acc

Translocation Breakpoints Are Clustered on Both Chromosome 8
Translocation Breakpoints Are Clustered on Both Chromosome 8

... library spanning most of the intron centromeric to the fifth reported AMLl exon.7 This is the region where chromosome 21 breakpoints were suggested to cluster in the t(8;21). As the intron contains a single BamHI site, rearrangements within it can be investigated using probes for the two fragments g ...
Glossary of Genetic Terms 11Jul15
Glossary of Genetic Terms 11Jul15

... Agrobacterium tumefaciens - A soil bacterium that infects plants where they are damaged. The bacterium parasite constructs a tunnel through which it delivers a parcel of its own genetic material into the plant cell. The parcel consist of a stretch of DNA that is excised from a plasmid and wrapped in ...
Chapter 13 Unintended Horizontal Transfer of Recombinant DNA
Chapter 13 Unintended Horizontal Transfer of Recombinant DNA

... organism involved (e.g. sexual reproduction/inheritance by descent). This process is called vertical gene transfer and an example is pollen flow between the same or related plant species.1 Thus, vertical gene transfer is the normal mode in which DNA is shared among individuals and passed on to the f ...
A.3.2.3BreastCancerElectrophoresis
A.3.2.3BreastCancerElectrophoresis

... STR is a region of DNA composed of a short sequence of nucleotides repeated many times. The number of repeated sequences in a given STR varies from person to person. The alternate forms of a given STR correspond with different alleles. Most STRs occur in gene introns (non-coding regions of DNA), so ...
doc THREE finals
doc THREE finals

... containing one chromosome 18, one cell containing two chromosomes 18 and one cell without any human chromosome 18. 5) Human males affected by the Klinefelter syndrome will have additional “X” chromosome(s), while human females affected by the Turner syndrome will lack one chromosome “X”. Based on th ...
ZFX has a Gene Structure Similar to ZFY, the Putative
ZFX has a Gene Structure Similar to ZFY, the Putative

The Drosophila Expression System.
The Drosophila Expression System.

... • Integrate multiple copies of expression plasmids to allow isolation of high-producing polyclonal stable cell lines In addition, endogenous Drosophila proteins generally do not interact with mammalian proteins, so S2 cells provide a “null background” for functional studies of proteins. ...
Opportunities in Bioinformatics for Computer - People
Opportunities in Bioinformatics for Computer - People

... • Potatoes originated in the Andes, where there are many varieties. • Many varieties survive at high altitude in cold, dry conditions. • Microarray technology can be used to investigate genes that are responsible for stress resistance and that are responsible for the production of nutrients. ...
Colorado Potato Breeding and Selection Program
Colorado Potato Breeding and Selection Program

... Figure 1. Results of PCR amplification of the H1 locus from several commercial potato varieties. Varieties followed by (R) are GN resistant based on phenotypic evidence, those followed by (S) are susceptible. Almost all resistant varieties exhibit the TG689 allele-specific band (141 bp); known excep ...
Tassia 1 Benthic Macrofauna Abundance Along a Transect from
Tassia 1 Benthic Macrofauna Abundance Along a Transect from

... structure (Netto & Lana 1997, Ysaebaert & Herman 2002, Beukema 1976). However, studies of community structure can require accurate identification of species. Thus, results may change depending on the researchers ability to correctly identify one small animal from another of similar form (Leasi & Nor ...
Overexpression of DNA repair genes is associated with metastasis
Overexpression of DNA repair genes is associated with metastasis

... related to metastasis [6–8]. Among these genes, we have particularly focused on those involved in the maintenance of genetic stability in human cells in order to determine whether variations in the expression of repair genes have any association with metastatic risk. On purpose, this review does not ...
1_Genbank
1_Genbank

... In This Chapter (see Ch.3 BfD): ...
Adenine - One of the four bases in DNA tha make up the letters
Adenine - One of the four bases in DNA tha make up the letters

... Agrobacterium tumefaciens - A soil bacterium that infects plants where they are damaged. The bacterium parasite constructs a tunnel through which it delivers a parcel of its own genetic material into the plant cell. The parcel consist of a stretch of DNA that is excised from a plasmid and wrapped in ...
The genome-scale interplay amongst xenogene silencing
The genome-scale interplay amongst xenogene silencing

Gene conversion analysis of the mouse Pilr locus
Gene conversion analysis of the mouse Pilr locus

... Gene conversion has played a role in shaping the mouse Pilr locus and is detected by a variety of phylogenetic and statistical methods. Phylogenetic analysis of a conserved 3 kb region (the promoter through to intron 3) suggests that Pilrb1 and Pilrb2 are more related to each other than they are to ...
Chromosomes and Inheritance - 1 Chromosome Theory of
Chromosomes and Inheritance - 1 Chromosome Theory of

... Chromosome mapping using recombination data was used extensively in the earlier part of the 20th century. Today we have much more sophisticated methods of dealing with chromosome mapping using DNA probes, where a known DNA fragment is used to compare with an unknown chromosome region. If the probe m ...
Modulation of base excision repair of 8
Modulation of base excision repair of 8

Things to know for the Final - Mercer Island School District
Things to know for the Final - Mercer Island School District

... Be able to explain how meiosis relates to genetics. What type of cells are produced? Be able to explain Mendel’s experiments. For example, when he crossed true-breeding tall plant seeds with true-breeding short plant seeds, why was the F1 generation all tall plants? Why did the F2 generation consist ...
Chapter 15 The Chromosomal Basis of Inheritance
Chapter 15 The Chromosomal Basis of Inheritance

... In humans and other mammals, there are two varieties of sex chromosomes: a larger X chromosome and a smaller Y chromosome Only the ends of the Y chromosome have regions that are homologous with corresponding regions of the X chromosome The SRY gene on the Y chromosome codes for a protein that direct ...
STAAR Review 3
STAAR Review 3

... b. Ear size is a trait that shows incomplete dominance. c. Ear size is controlled by the environment. d. Ear size is not genetically controlled. ...
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Genomic library



A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.
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