Technical Targets
... These gene expression data could not rule out the possibility that hyp2 genes or any additional genes in the CBS genome are capable of assisting the maturation of the CBS hydrogenase. To address this question we took a genetic approach and created ΔhypE1, ΔhypE2, and ΔhypE1:ΔhypE2 mutant strains. Th ...
... These gene expression data could not rule out the possibility that hyp2 genes or any additional genes in the CBS genome are capable of assisting the maturation of the CBS hydrogenase. To address this question we took a genetic approach and created ΔhypE1, ΔhypE2, and ΔhypE1:ΔhypE2 mutant strains. Th ...
Network properties of human disease genes with pleiotropic effects
... Full list of author information is available at the end of the article ...
... Full list of author information is available at the end of the article ...
Polymorphisms in Multiple Genes Contribute to the
... The single-allelic replacement strains were all of the same mating type. After several crosses and sporulations, we constructed a diploid strain that is homozygous for MKT130G and heterozygous for SAL1/sal1-1, CAT5-91M/CAT5-91I, and MIP1-661T/MIP1-661A. All the strains in Figure 7 are haploid spores ...
... The single-allelic replacement strains were all of the same mating type. After several crosses and sporulations, we constructed a diploid strain that is homozygous for MKT130G and heterozygous for SAL1/sal1-1, CAT5-91M/CAT5-91I, and MIP1-661T/MIP1-661A. All the strains in Figure 7 are haploid spores ...
Reliable classification of two-class cancer data using evolutionary
... K. Deb, A. Raji Reddy / BioSystems 72 (2003) 111–129 ...
... K. Deb, A. Raji Reddy / BioSystems 72 (2003) 111–129 ...
Genomics-based approaches to improve drought tolerance of crops
... Reverse genetics: a method of investigating the genetic control of traits that begins with the nucleotide sequence of a gene and attempts to establish its function by generating and testing mutants. SNP (single nucleotide polymorphism): a point mutation that can be targeted by molecular techniques a ...
... Reverse genetics: a method of investigating the genetic control of traits that begins with the nucleotide sequence of a gene and attempts to establish its function by generating and testing mutants. SNP (single nucleotide polymorphism): a point mutation that can be targeted by molecular techniques a ...
Mutation Analysis of the Ornithine Transcarbamylase (OTC) Gene in
... sequences. Therefore, OTC mRNA in the liver was analyzed by RT-PCR, and remarkably, a 135-nt insertion was detected between exons 5 and 6. Genomic DNA analysis of intron sequences revealed a single nucleotide change at 265 bp downstream from the 3’ end of exon 5, which created the novel splice accep ...
... sequences. Therefore, OTC mRNA in the liver was analyzed by RT-PCR, and remarkably, a 135-nt insertion was detected between exons 5 and 6. Genomic DNA analysis of intron sequences revealed a single nucleotide change at 265 bp downstream from the 3’ end of exon 5, which created the novel splice accep ...
wsp Gene Sequences from the Wolbachia of Filarial Nematodes
... The PCR products obtained with primers WSPintF and WSPintR from nematode Wolbachia were sequenced directly, and the sequences were aligned to the wsp gene available for arthropod Wolbachia. We also tried to align wsp to the gene sequences available for the major outer membrane proteins of Anaplasma, ...
... The PCR products obtained with primers WSPintF and WSPintR from nematode Wolbachia were sequenced directly, and the sequences were aligned to the wsp gene available for arthropod Wolbachia. We also tried to align wsp to the gene sequences available for the major outer membrane proteins of Anaplasma, ...
Supplementary Infomation (doc 1650K)
... downstream of rs12649507, earlier associated with SDav1) for associations with SDav in the discovery meta-analysis (−log10 P values on the y axis and the SNP genomic position on the x axis). We could confirm the earlier association of rs12649507 or rs11932595 with SDav (only had imputed genotypes) i ...
... downstream of rs12649507, earlier associated with SDav1) for associations with SDav in the discovery meta-analysis (−log10 P values on the y axis and the SNP genomic position on the x axis). We could confirm the earlier association of rs12649507 or rs11932595 with SDav (only had imputed genotypes) i ...
The amphioxus hairy family: differential fate after duplication.
... Several representatives of the hairy family have been so far isolated from a range of protostome and vertebrate species. In protostomes such as Drosophila they have been implicated in segmentation, peripheral nervous system development, and in somatic sex determination. In Tribolium, only a role dur ...
... Several representatives of the hairy family have been so far isolated from a range of protostome and vertebrate species. In protostomes such as Drosophila they have been implicated in segmentation, peripheral nervous system development, and in somatic sex determination. In Tribolium, only a role dur ...
The molecular genetics of head development in Drosophila
... embryonic head structures. The adult head of Drosophila is formed from three pairs of imaginal discs which develop during larval stages. Most head structures, including the head capsule, eyes, antennae and maxillary palp are derived from the eye-antennal discs (Bryant, 1978). The proboscis and the c ...
... embryonic head structures. The adult head of Drosophila is formed from three pairs of imaginal discs which develop during larval stages. Most head structures, including the head capsule, eyes, antennae and maxillary palp are derived from the eye-antennal discs (Bryant, 1978). The proboscis and the c ...
CRISPR-Cas9 Mouse Toolbox
... of adenocarcinoma pathology. These plasmids as well as a backbone plasmid for cloning new targets are described here. 1. AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-CreshortPA-KrasG12D_HDRdonor-ITR (AAV-KPL) This plasmid contains two expression cassettes, Renilla luciferase-2A-C ...
... of adenocarcinoma pathology. These plasmids as well as a backbone plasmid for cloning new targets are described here. 1. AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-CreshortPA-KrasG12D_HDRdonor-ITR (AAV-KPL) This plasmid contains two expression cassettes, Renilla luciferase-2A-C ...
Document
... • Where is the SNP mapped? Exon, promoter, UTR, etc picture of gene with mapped to the gene structure. • How was it discovered? Method • What assurances do you have that it is real? Validated how? • What population – African, European, etc? • What is the allele frequency of each SNP? Common (>10%) ...
... • Where is the SNP mapped? Exon, promoter, UTR, etc picture of gene with mapped to the gene structure. • How was it discovered? Method • What assurances do you have that it is real? Validated how? • What population – African, European, etc? • What is the allele frequency of each SNP? Common (>10%) ...
Dominant and Recessive Genes
... equal ratios. One kind of gamete carries the dominant gene and the other kind carries the recessive gene. The two kinds of gametes produced by one parent each have an equal chance of uniting with each of the two types of gametes produced by the other parent. Thus, four equal chance unions of gametes ...
... equal ratios. One kind of gamete carries the dominant gene and the other kind carries the recessive gene. The two kinds of gametes produced by one parent each have an equal chance of uniting with each of the two types of gametes produced by the other parent. Thus, four equal chance unions of gametes ...
The Optimal Discovery Procedure II: Applications to Comparative
... A microarray measures the abundance of mRNA transcripts of thousands of genes from a single biological sample (1, 2). Of much recent interest has been the problem of identifying genes that are differentially expressed across varying biological conditions based on microarray data (3). It is now possi ...
... A microarray measures the abundance of mRNA transcripts of thousands of genes from a single biological sample (1, 2). Of much recent interest has been the problem of identifying genes that are differentially expressed across varying biological conditions based on microarray data (3). It is now possi ...
Advances in genetics show the need for extending screening
... (threshold for complex traits), since this means that a large number of individuals is needed for the analysis.15,16 Another means of identifying novel ADH genes is through genome-wide association studies. This approach received substantial interest in the last few years. For ADH, several new candid ...
... (threshold for complex traits), since this means that a large number of individuals is needed for the analysis.15,16 Another means of identifying novel ADH genes is through genome-wide association studies. This approach received substantial interest in the last few years. For ADH, several new candid ...
The genomic landscape of meiotic crossovers and gene
... exchange of complete chromosome arms, or non-crossovers (NCOs). In both cases, heteroduplexes may arise from sequence divergence at the site of the strand invasion. When these are resolved, they can give rise to gene conversions (GCs), the non-reciprocal exchange of alleles between homologous non-si ...
... exchange of complete chromosome arms, or non-crossovers (NCOs). In both cases, heteroduplexes may arise from sequence divergence at the site of the strand invasion. When these are resolved, they can give rise to gene conversions (GCs), the non-reciprocal exchange of alleles between homologous non-si ...
Identification of New Genes Involved in Meiosis by a Genetic Screen
... The yeast spore wall is a thick four-layered structure. Dityrosine is a major component of the spore wall that provides resistance against digestive enzymes such as glusalase, as well as UV and are present at the outermost and last synthesized layer of the spore wall (Briza et al., 1986; Pammer et a ...
... The yeast spore wall is a thick four-layered structure. Dityrosine is a major component of the spore wall that provides resistance against digestive enzymes such as glusalase, as well as UV and are present at the outermost and last synthesized layer of the spore wall (Briza et al., 1986; Pammer et a ...
Comparative analysis of two-component signal transduction systems
... analysis, a footprint of these proteins was made. Around 40 HK-RR gene pairs were detected in each member of the B. cereus group. In addition, each member contained many HK and RR genes not encoded in pairs (‘orphans’). Classification of HKs and RRs based on their enzymic domains together with the a ...
... analysis, a footprint of these proteins was made. Around 40 HK-RR gene pairs were detected in each member of the B. cereus group. In addition, each member contained many HK and RR genes not encoded in pairs (‘orphans’). Classification of HKs and RRs based on their enzymic domains together with the a ...
Simplified Insertion of Transgenes Onto Balancer Chromosomes via
... ABSTRACT Balancer chromosomes are critical tools for Drosophila genetics. Many useful transgenes are inserted onto balancers using a random and inefficient process. Here we describe balancer chromosomes that can be directly targeted with transgenes of interest via recombinase-mediated cassette exchan ...
... ABSTRACT Balancer chromosomes are critical tools for Drosophila genetics. Many useful transgenes are inserted onto balancers using a random and inefficient process. Here we describe balancer chromosomes that can be directly targeted with transgenes of interest via recombinase-mediated cassette exchan ...
Human Genetic Variation Databases Workshop - Cordis
... An workshop of leading European bioinformaticians, biologists, medical researchers and clinicians was held in Brussels on 2-3 March 2006 to examine if strategies could be identified. The conclusion was that an integrated database and analysis structure for much of human and model organism variation ...
... An workshop of leading European bioinformaticians, biologists, medical researchers and clinicians was held in Brussels on 2-3 March 2006 to examine if strategies could be identified. The conclusion was that an integrated database and analysis structure for much of human and model organism variation ...