FocalScan: Scanning for altered genes in cancer based on
... oncogenesis (oncogenes) or hinder cancer growth (tumor suppressors). Tools have therefore been developed to find these frequently altered regions (2,3). However, the focal regions identified by existing tools often span a large number of genes, and many times it is not clear which gene is the target ...
... oncogenesis (oncogenes) or hinder cancer growth (tumor suppressors). Tools have therefore been developed to find these frequently altered regions (2,3). However, the focal regions identified by existing tools often span a large number of genes, and many times it is not clear which gene is the target ...
DNA Prokaryote Transcription Steps (updated February 2013)
... promoter. The promoter has several sequences that are similar to the Pribnow and TTGACA boxes in prokaryote promoters. The TATA box (TATAAA) is almost identical to the Pribnow sequence. Only about 32% of the known eukaryote core promoters have the TATA box at -26 to -31. If the iniator (Inr) core pr ...
... promoter. The promoter has several sequences that are similar to the Pribnow and TTGACA boxes in prokaryote promoters. The TATA box (TATAAA) is almost identical to the Pribnow sequence. Only about 32% of the known eukaryote core promoters have the TATA box at -26 to -31. If the iniator (Inr) core pr ...
Full text in pdf - International Microbiology
... Summary. The non-phosphorylating glyceraldehyde 3-phosphate dehydrogenase (GAPDHN, NADP+-specific, EC 1.2.1.9) is present in green eukaryotes and some Streptococcus strains. The present report describes the results of activity and immunoblot analyses, which were used to generate the first survey of ...
... Summary. The non-phosphorylating glyceraldehyde 3-phosphate dehydrogenase (GAPDHN, NADP+-specific, EC 1.2.1.9) is present in green eukaryotes and some Streptococcus strains. The present report describes the results of activity and immunoblot analyses, which were used to generate the first survey of ...
pdf
... section of Cluster I (Fig. 3). These were most closely related to sequences derived from other marine environments, such as the South China Sea and the English Channel. Of particular interest is the near perfect identity of several clones from 150 and 175 m at Station 1 (S1D150M21 and S1D175M21) wit ...
... section of Cluster I (Fig. 3). These were most closely related to sequences derived from other marine environments, such as the South China Sea and the English Channel. Of particular interest is the near perfect identity of several clones from 150 and 175 m at Station 1 (S1D150M21 and S1D175M21) wit ...
Examination of the molecular control of uterine function
... A DNAseq approach incorporating DNA pooling, target enrichment and high throughout sequencing was applied to identify polymorphisms and estimate allele frequencies in 10 candidate genes found to be differentially expressed in microarray studies. DNA samples from 150 Holstein-Friesian bulls divided i ...
... A DNAseq approach incorporating DNA pooling, target enrichment and high throughout sequencing was applied to identify polymorphisms and estimate allele frequencies in 10 candidate genes found to be differentially expressed in microarray studies. DNA samples from 150 Holstein-Friesian bulls divided i ...
microbial methanol s.. - Chemical and Biomolecular Engineering
... • Oxidize methane to methanol via the nonspecific action of the enzyme ammonia monooxygenase • Contaminants such as moisture and CO2 do not post a limitation for biological conversion • Can utilize the CO2 contained in gas mixtures for cell synthesis ...
... • Oxidize methane to methanol via the nonspecific action of the enzyme ammonia monooxygenase • Contaminants such as moisture and CO2 do not post a limitation for biological conversion • Can utilize the CO2 contained in gas mixtures for cell synthesis ...
Document
... Odds of linked = the chance that you saw the pedigree data because the trait locus and the SSR were linked Odds of NOT linked = the chance that you saw the pedigree data because the trait locus and the SSR were NOT linked ...
... Odds of linked = the chance that you saw the pedigree data because the trait locus and the SSR were linked Odds of NOT linked = the chance that you saw the pedigree data because the trait locus and the SSR were NOT linked ...
11357_2014_9648_MOESM1_ESM
... liver RNA was used to synthesize single-stranded complementary DNA (cDNA). Therefore, the “First Strand cDNA synthesis Kit” (Fermentas, Thermo Scientific) was used following the supplier's protocol for random hexamer primers. Resulting cDNA was stored at -20°C until further use. Quantitative real-ti ...
... liver RNA was used to synthesize single-stranded complementary DNA (cDNA). Therefore, the “First Strand cDNA synthesis Kit” (Fermentas, Thermo Scientific) was used following the supplier's protocol for random hexamer primers. Resulting cDNA was stored at -20°C until further use. Quantitative real-ti ...
Aucun titre de diapositive - Universidad Nacional De Colombia
... The algorithm looks for the co-occurrence of n-length words (n = 6) in a window of size 150 bases having at least 96% identity. Sequences shorter than 50 bases are excluded from the clustering process. Clusters highly related sequences. Clusters also sequences related by rearrangements or alternativ ...
... The algorithm looks for the co-occurrence of n-length words (n = 6) in a window of size 150 bases having at least 96% identity. Sequences shorter than 50 bases are excluded from the clustering process. Clusters highly related sequences. Clusters also sequences related by rearrangements or alternativ ...
Nucleotide substitutions and evolution of duplicate genes.
... In a large-scale analysis involving several pairs of genes the sequences in one pair have a different length than sequences in another pair. Thus the goal is to compute the number of substitutions per site or number of substitutions per codon. In our search for duplicate genes we used two different ...
... In a large-scale analysis involving several pairs of genes the sequences in one pair have a different length than sequences in another pair. Thus the goal is to compute the number of substitutions per site or number of substitutions per codon. In our search for duplicate genes we used two different ...
Document
... -!- DISEASE: Defects in FOXP2 are the cause of speech-language disorder 1 (SPCH1) [MIM:602081]; also known as autosomal dominant speech and language disorder with orofacial dyspraxia. Affected individuals have a severe impairment in the selection and sequencing of fine orofacial movements, which are ...
... -!- DISEASE: Defects in FOXP2 are the cause of speech-language disorder 1 (SPCH1) [MIM:602081]; also known as autosomal dominant speech and language disorder with orofacial dyspraxia. Affected individuals have a severe impairment in the selection and sequencing of fine orofacial movements, which are ...
Chapter 17: Transcription, RNA Processing, and Translation
... 3.) What are the components that make up the bacterial RNA Polymerase Holoenzyme? What is the function of each component? 4.) What is the significance of the -35 box, -10 box, and +1 box? In bacteria, what component of the RNA Polymerase holoenzyme interacts with the DNA initially during transcripti ...
... 3.) What are the components that make up the bacterial RNA Polymerase Holoenzyme? What is the function of each component? 4.) What is the significance of the -35 box, -10 box, and +1 box? In bacteria, what component of the RNA Polymerase holoenzyme interacts with the DNA initially during transcripti ...
Development of a molecular genetic diagnostic service for X
... Probes for each of 10 exons Other probes include KAL1 and NLGN4X In female heterozygotes, 35-50% reduced relative peak area of amplified product expected Deletion of one exon – needs to be confirmed by sequencing to rule out mutation/ polymorphism close to probe ligation site ...
... Probes for each of 10 exons Other probes include KAL1 and NLGN4X In female heterozygotes, 35-50% reduced relative peak area of amplified product expected Deletion of one exon – needs to be confirmed by sequencing to rule out mutation/ polymorphism close to probe ligation site ...
13.2 abbreviated Interactive Text
... vitamins in certain crops. That will help provide better nutrition. Some plants have already been developed that produce toxins to make them resistant to insects. That will limit the use of dangerous pesticides. ...
... vitamins in certain crops. That will help provide better nutrition. Some plants have already been developed that produce toxins to make them resistant to insects. That will limit the use of dangerous pesticides. ...
Table 1
... The evolution of genomic technologies is occurring rapidly and often requires large amounts of source DNA. There is also an expanded desire to analyze smaller numbers of cells for higher resolution studies as well as to take advantage of large numbers of archived samples (eg. FFPE, serum, etc.). To ...
... The evolution of genomic technologies is occurring rapidly and often requires large amounts of source DNA. There is also an expanded desire to analyze smaller numbers of cells for higher resolution studies as well as to take advantage of large numbers of archived samples (eg. FFPE, serum, etc.). To ...
Origin and evolution of the slime molds (Mycetozoa)
... PCR-generated sequences. The Toxoplasma gondii tef, encoding '75% of the EF-1a protein, was compiled from the EST (expressed sequence tag) database, using only those regions for which at least two ESTs were available. Preliminary distance trees (see below) of all available sequences were used to tri ...
... PCR-generated sequences. The Toxoplasma gondii tef, encoding '75% of the EF-1a protein, was compiled from the EST (expressed sequence tag) database, using only those regions for which at least two ESTs were available. Preliminary distance trees (see below) of all available sequences were used to tri ...
Supplemental Figure 1. Floral commitment in Arabidopsis WT and
... reads, i.e., reads that start with the exact same sequence and could be artifacts from multiple sequencing of the same molecule. The remaining reads were mapped to the TAIR9 genome with bowtie (Langmead et al., 2009), allowing for three mismatches and only uniquely mapping reads were counted. The un ...
... reads, i.e., reads that start with the exact same sequence and could be artifacts from multiple sequencing of the same molecule. The remaining reads were mapped to the TAIR9 genome with bowtie (Langmead et al., 2009), allowing for three mismatches and only uniquely mapping reads were counted. The un ...
Full Text - Genes | Genomes | Genetics
... DNA/RNA metabolism, protein folding, trafficking, and degradation; Chervitz et al. 1998). The major limitation of this study was that yeast and worm were the only complete eukaryotic genome sequences available at the time. Today, hundreds of diverse genomes have been sequenced. However, not all these ...
... DNA/RNA metabolism, protein folding, trafficking, and degradation; Chervitz et al. 1998). The major limitation of this study was that yeast and worm were the only complete eukaryotic genome sequences available at the time. Today, hundreds of diverse genomes have been sequenced. However, not all these ...
Bioinformatics Dr. Víctor Treviño Pabellón Tec
... a tree is referred to as the tree length. The tree is also a bifurcating or binary tree, in that only two branches emanate from each node. Trees can have more than one branch emanating from a node if the events separating taxa are so close that they cannot be resolved, or to simplify the tree. The u ...
... a tree is referred to as the tree length. The tree is also a bifurcating or binary tree, in that only two branches emanate from each node. Trees can have more than one branch emanating from a node if the events separating taxa are so close that they cannot be resolved, or to simplify the tree. The u ...
MacVector 14.0 Getting Started Guide
... can be selected from the keyword list when editing the features of protein or nucleic acid sequences. ...
... can be selected from the keyword list when editing the features of protein or nucleic acid sequences. ...
Teaching deep time through macroevolution and
... 3. Students align sequences, calculate the number of differences among taxa and use a computer program to generate a phylogenetic tree [more complex iteration of initial exercise; introduces bioinformatics] 4. Students download cytb sequence and generate another tree for the same taxa [repeat tree m ...
... 3. Students align sequences, calculate the number of differences among taxa and use a computer program to generate a phylogenetic tree [more complex iteration of initial exercise; introduces bioinformatics] 4. Students download cytb sequence and generate another tree for the same taxa [repeat tree m ...
gene-environment interaction and twin studies
... significance level. 6 A forward-looking (and rich!) gene-hunting company would be obtaining good phenotypes on large numbers of MZ twins against the day (soon promised) when it will be a cheap option to scan the genome for tens of thousands ofclosely spaced SNP's, any ofwhich may be (in linkage dise ...
... significance level. 6 A forward-looking (and rich!) gene-hunting company would be obtaining good phenotypes on large numbers of MZ twins against the day (soon promised) when it will be a cheap option to scan the genome for tens of thousands ofclosely spaced SNP's, any ofwhich may be (in linkage dise ...
Analyzing stochastic transcription to elucidate the nucleoid`s
... chromosome (a gene distance of around 650). Similarly, Figures 1b and 1c show the results for the chromosome and the megaplasmid pSymB, respectively, when both are actively transcribed (and translated; data set A). As can be seen, the autocorrelation functions for the three replicons are similar. No ...
... chromosome (a gene distance of around 650). Similarly, Figures 1b and 1c show the results for the chromosome and the megaplasmid pSymB, respectively, when both are actively transcribed (and translated; data set A). As can be seen, the autocorrelation functions for the three replicons are similar. No ...
AOAC 2009.03 and Assurance GDSTD Salmonella Tq method
... highly conserved DNA sequence. Assurance GDSTD Salmonella Tq is a modified version of AOAC 2009.03 whereby the polymerase enzyme, instead of being added separately by the analyst, is lyophilized with the other PCR reagents inside the amplification tube at the time of manufacturing. Detection of Salm ...
... highly conserved DNA sequence. Assurance GDSTD Salmonella Tq is a modified version of AOAC 2009.03 whereby the polymerase enzyme, instead of being added separately by the analyst, is lyophilized with the other PCR reagents inside the amplification tube at the time of manufacturing. Detection of Salm ...
DNA Technology Notes
... This works because all organisms use the same genetic code read genes the same ...
... This works because all organisms use the same genetic code read genes the same ...
Metagenomics
Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.