ppt
... paml can be used to find the maximum likelihood tree, however, the program is rather slow. Phyml is a better choice to find the tree, which then can be used as a user tree. An example for a codeml.ctl file is codeml.hv1.sites.ctl This file directs codeml to run three different models: one with an om ...
... paml can be used to find the maximum likelihood tree, however, the program is rather slow. Phyml is a better choice to find the tree, which then can be used as a user tree. An example for a codeml.ctl file is codeml.hv1.sites.ctl This file directs codeml to run three different models: one with an om ...
GDP-HiFi DNA Polymerase
... GDP-HiFi is a new recombinant enzyme with genetic modification for its amino acid sequence, which results 70 times better fidelity than Taq DNA polymerase and an extremely fast elongation rate (as fast as 15 seconds per kb). GDP-HiFi has higher stability at high temperature. Users may program the init ...
... GDP-HiFi is a new recombinant enzyme with genetic modification for its amino acid sequence, which results 70 times better fidelity than Taq DNA polymerase and an extremely fast elongation rate (as fast as 15 seconds per kb). GDP-HiFi has higher stability at high temperature. Users may program the init ...
GEDI Input Data Format - Boston Children`s Hospital
... Column 1: Start with symbol “}”, “Dynamic” for time series analysis, “Static” for static analysis. Column 2: “DESC”, for gene description, this column is optional. Column 3: Time Unit – it can be a unit of the time point (hour or minute or day) for dynamic analysis. For static analysis, it is not ap ...
... Column 1: Start with symbol “}”, “Dynamic” for time series analysis, “Static” for static analysis. Column 2: “DESC”, for gene description, this column is optional. Column 3: Time Unit – it can be a unit of the time point (hour or minute or day) for dynamic analysis. For static analysis, it is not ap ...
GENE TECHNOLOGY - mf011
... Gene cloning involves using bacteria to make multiple copies of a gene Foreign DNA is inserted into a plasmid, and the recombinant plasmid is inserted into a bacterial cell Reproduction in the bacterial cell results in cloning of the plasmid including the foreign DNA This results in the production o ...
... Gene cloning involves using bacteria to make multiple copies of a gene Foreign DNA is inserted into a plasmid, and the recombinant plasmid is inserted into a bacterial cell Reproduction in the bacterial cell results in cloning of the plasmid including the foreign DNA This results in the production o ...
Current and Future Developments in Genomics
... 2.1 Sequencing and annotation By the end of 2006, the genome sequence of many important and interesting organisms had been published, with still further genome sequences held in private and other organisms such as the gorilla due to have their genomic sequence released in the near future. For many o ...
... 2.1 Sequencing and annotation By the end of 2006, the genome sequence of many important and interesting organisms had been published, with still further genome sequences held in private and other organisms such as the gorilla due to have their genomic sequence released in the near future. For many o ...
E.coli mikrosirujen analyysi
... • each spot contains specific nucleotide sequences, probes • in hybridization process, labeled (Cy5, Cy3) samples attach to probes • comparative genome hybridization (CGH): DNA samples • gene expression: RNA samples • relative intensity of hybridization can be measured Panu Somervuo, March 19, 2007 ...
... • each spot contains specific nucleotide sequences, probes • in hybridization process, labeled (Cy5, Cy3) samples attach to probes • comparative genome hybridization (CGH): DNA samples • gene expression: RNA samples • relative intensity of hybridization can be measured Panu Somervuo, March 19, 2007 ...
1471-2164-12-165
... However, the gene model predicted by RGP track (P0030G11.20.spp) is different from others. (h) In indica chr10:13537529-13541502 area, the genes predicted by RAP (Os11t0237700-01), RGP (OSJNBb0023E20.8), BGF (BGIOSIBCE032114.1) and FgeneSH (BGIOSIFCE032530.1) are similar, and they are supported by E ...
... However, the gene model predicted by RGP track (P0030G11.20.spp) is different from others. (h) In indica chr10:13537529-13541502 area, the genes predicted by RAP (Os11t0237700-01), RGP (OSJNBb0023E20.8), BGF (BGIOSIBCE032114.1) and FgeneSH (BGIOSIFCE032530.1) are similar, and they are supported by E ...
Table of Contents - Milan Area Schools
... isolated and incubated with reverse transcriptase (RT) to make complementary DNA (cDNA). The cDNA is amplified by PCR prior to hybridization. The amplified cDNA is coupled to a fluorescent dye and then hybridized to the chip. A scanner detects glowing spots on the array. The combinations of thes ...
... isolated and incubated with reverse transcriptase (RT) to make complementary DNA (cDNA). The cDNA is amplified by PCR prior to hybridization. The amplified cDNA is coupled to a fluorescent dye and then hybridized to the chip. A scanner detects glowing spots on the array. The combinations of thes ...
Bacteria / Monerans
... • Can engage in primitive sexual reproduction called conjugation. – A bridge is formed between 2 bacteria – Plasmids are shared across bridge, thus exchanging genetic information. – In times of harsh conditions, bacteria can form endospores, to encapsulate themselves in a dormant state, until condit ...
... • Can engage in primitive sexual reproduction called conjugation. – A bridge is formed between 2 bacteria – Plasmids are shared across bridge, thus exchanging genetic information. – In times of harsh conditions, bacteria can form endospores, to encapsulate themselves in a dormant state, until condit ...
CosMicGBrowseSept2010
... http://www.sanger.ac.uk/genetics/CGP/cosmic Sources of mutation data 1. Literature (curators) 2. Other database(s) eg TP53 (IARC) International Agency for Research on Cancer 3. Sequencing/mutation detection • 2010: COSMIC GBrowse (22nd September??) http://www.sanger.ac.uk/fgb2/gbrowse/cosmic x ...
... http://www.sanger.ac.uk/genetics/CGP/cosmic Sources of mutation data 1. Literature (curators) 2. Other database(s) eg TP53 (IARC) International Agency for Research on Cancer 3. Sequencing/mutation detection • 2010: COSMIC GBrowse (22nd September??) http://www.sanger.ac.uk/fgb2/gbrowse/cosmic x ...
Challenging the dogma: the hidden layer of non-protein
... spliced. In addition, there are over 2,400 pairs of overlapping sense-antisense transcripts, of which almost 1,600 had not previously been identified,(18) an apparently common phenomenon.(48) Some of these transcripts, such as novel antisense transcripts from the imprinted Gnas locus, have since bee ...
... spliced. In addition, there are over 2,400 pairs of overlapping sense-antisense transcripts, of which almost 1,600 had not previously been identified,(18) an apparently common phenomenon.(48) Some of these transcripts, such as novel antisense transcripts from the imprinted Gnas locus, have since bee ...
Microbial Quality of Unregulated Herbal Medicinal Products in Kenya
... Background: The use of herbal medicines is global, with the majority of the world’s population depending on traditional medicines, particularly herbal remedies for their primary healthcare needs. In Kenya, it is estimated that conventional healthcare system provides for approximately 30 % of the pop ...
... Background: The use of herbal medicines is global, with the majority of the world’s population depending on traditional medicines, particularly herbal remedies for their primary healthcare needs. In Kenya, it is estimated that conventional healthcare system provides for approximately 30 % of the pop ...
Genotyping of Ryanodine receptor 1 (RYR1) gene associated with
... with temperature shift assay and allele- specific PCR (Tm-shift AS-PCR HRM) for amplifying the differences in DNA melting profiles between different genotypes of the gene. Firstly, classic PCR-restriction fragment length polymorphism (PCR-RFLP) was examined to detect RYR1 polymorphisms by determinin ...
... with temperature shift assay and allele- specific PCR (Tm-shift AS-PCR HRM) for amplifying the differences in DNA melting profiles between different genotypes of the gene. Firstly, classic PCR-restriction fragment length polymorphism (PCR-RFLP) was examined to detect RYR1 polymorphisms by determinin ...
Proteorhodopsin Phototrophy Promotes Survival of Marine
... elements since transposase genes are found flanking the PR, crtEIBY, and blh genes in both BAA-1116 and AND4 (Figure 2). The transposase gene closest to the PR gene in AND4 was truncated and showed best matches to transposases in V. anguillarum 775, V. parahaemolyticus AQ3776 and V. cholerae 91, wit ...
... elements since transposase genes are found flanking the PR, crtEIBY, and blh genes in both BAA-1116 and AND4 (Figure 2). The transposase gene closest to the PR gene in AND4 was truncated and showed best matches to transposases in V. anguillarum 775, V. parahaemolyticus AQ3776 and V. cholerae 91, wit ...
Microbial Evolution and Diversity
... higher priority. For example, in traditional taxonomy, the Gram stain has been given more weight in determining the classification of an organism than whether the organism uses glucose as a carbon source. Therefore, all gram-positive strains would be ascribed to one family or genus, and, within that ...
... higher priority. For example, in traditional taxonomy, the Gram stain has been given more weight in determining the classification of an organism than whether the organism uses glucose as a carbon source. Therefore, all gram-positive strains would be ascribed to one family or genus, and, within that ...
File
... • Plasmid pBR322 has been a widely used cloning vehicle. • In addition, it has been widely used as a model system for the study of prokaryotic transcription and translation, as well as investigation of the effects of topological changes on DNA conformation. • The popularity of pBR322 is a direct res ...
... • Plasmid pBR322 has been a widely used cloning vehicle. • In addition, it has been widely used as a model system for the study of prokaryotic transcription and translation, as well as investigation of the effects of topological changes on DNA conformation. • The popularity of pBR322 is a direct res ...
Document
... BLASTs to be performed and to speed the process, we downloaded the text or “flat file” of the TIGR rice protein sequences (available at: http://www.tigr.org/tdb/e2k1/osa1/data_download.shtml) and performed local blasts using blastall from NCBI (available at: http://www.ncbi.nlm.nih.gov/BLAST/downloa ...
... BLASTs to be performed and to speed the process, we downloaded the text or “flat file” of the TIGR rice protein sequences (available at: http://www.tigr.org/tdb/e2k1/osa1/data_download.shtml) and performed local blasts using blastall from NCBI (available at: http://www.ncbi.nlm.nih.gov/BLAST/downloa ...
Downloads - BioMed Central
... The Obesity Gene Map web site was developed to address the need to include increasingly detailed information on the location and properties of an increasing number of obesity-related genes. Data from the authors’ published reviews was used as the starting point for constructing the Obesity Gene Map ...
... The Obesity Gene Map web site was developed to address the need to include increasingly detailed information on the location and properties of an increasing number of obesity-related genes. Data from the authors’ published reviews was used as the starting point for constructing the Obesity Gene Map ...
A systematic genome-wide analysis of zebrafish protein
... Since the publication of the human reference genome, the identities of specific genes associated with human diseases are being discovered at an enormous rate. A central problem is that the biological activity of these genes is often unclear. Detailed investigations in vertebrate model organisms, typ ...
... Since the publication of the human reference genome, the identities of specific genes associated with human diseases are being discovered at an enormous rate. A central problem is that the biological activity of these genes is often unclear. Detailed investigations in vertebrate model organisms, typ ...
Ancient human microbiomes
... lengths and a shift of focus to individual 16S rRNA gene hypervariable regions rather than the entire gene, the increased sequencing depth has revealed a great diversity of low abundance taxa within the human microbiome, expanding the known taxonomic complexity of the oral microbiome alone by more t ...
... lengths and a shift of focus to individual 16S rRNA gene hypervariable regions rather than the entire gene, the increased sequencing depth has revealed a great diversity of low abundance taxa within the human microbiome, expanding the known taxonomic complexity of the oral microbiome alone by more t ...
Phylogenetic Portrait of the Saccharomyces cerevisiae Functional
... DNA/RNA metabolism, protein folding, trafficking, and degradation; Chervitz et al. 1998). The major limitation of this study was that yeast and worm were the only complete eukaryotic genome sequences available at the time. Today, hundreds of diverse genomes have been sequenced. However, not all these ...
... DNA/RNA metabolism, protein folding, trafficking, and degradation; Chervitz et al. 1998). The major limitation of this study was that yeast and worm were the only complete eukaryotic genome sequences available at the time. Today, hundreds of diverse genomes have been sequenced. However, not all these ...
Lecture 35: Basics of DNA Cloning-I
... bacteriophage as a vector, a DNA fragment of size up to 20 kb can be transformed. Bacterial artificial chromosomes (BACs): Bacterial artificial chromosomes (BACs) are simple plasmid which is designed to clone very large DNA fragments ranging in size from 75 to 300 kb. BACs basically have marker like ...
... bacteriophage as a vector, a DNA fragment of size up to 20 kb can be transformed. Bacterial artificial chromosomes (BACs): Bacterial artificial chromosomes (BACs) are simple plasmid which is designed to clone very large DNA fragments ranging in size from 75 to 300 kb. BACs basically have marker like ...
IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) e-ISSN: 2278-3008, p-ISSN:2319-7676.
... ligated to a cloning vector pGEM-T and was cloned into an E.coli strain DH5α. The positive clones were screened for the cry gene content. The partial sequencing of the cloned gene from this strain was accomplished and sequence homology was analyzed. The alignment of the sequenced gene with gene sequ ...
... ligated to a cloning vector pGEM-T and was cloned into an E.coli strain DH5α. The positive clones were screened for the cry gene content. The partial sequencing of the cloned gene from this strain was accomplished and sequence homology was analyzed. The alignment of the sequenced gene with gene sequ ...
The MetaCyc database of metabolic pathways
... (PGDBs), each containing the full genome and predicted metabolic network of one organism, including metabolites, enzymes, reactions, metabolic pathways, predicted operons, transport systems, and pathway-hole fillers. The BioCyc website offers a variety of tools for querying and analyzing PGDBs, incl ...
... (PGDBs), each containing the full genome and predicted metabolic network of one organism, including metabolites, enzymes, reactions, metabolic pathways, predicted operons, transport systems, and pathway-hole fillers. The BioCyc website offers a variety of tools for querying and analyzing PGDBs, incl ...
Metagenomics
Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.