The genomic substrate for adaptive radiation in African cichlid fish
... ALLPATHS-LG1 (Extended Data 1a). Data from the sequencing instruments was imported directly into the program, without any filtering or other preprocessing. In brief, the ALLPATHSLG algorithm then proceeded by correction of sequencing errors within reads, closure of shortfragment read pairs, formatio ...
... ALLPATHS-LG1 (Extended Data 1a). Data from the sequencing instruments was imported directly into the program, without any filtering or other preprocessing. In brief, the ALLPATHSLG algorithm then proceeded by correction of sequencing errors within reads, closure of shortfragment read pairs, formatio ...
Next-Generation Sequencing applied to aDNA
... The makefile is specified using YAML, a human-readable markup language that is visually similar to Python code. In other words, the structure is defined using indentation. Note that tabs cannot be used when editing this file, always use spaces! A copy of the final makefile (“final_makefile.yaml”) wa ...
... The makefile is specified using YAML, a human-readable markup language that is visually similar to Python code. In other words, the structure is defined using indentation. Note that tabs cannot be used when editing this file, always use spaces! A copy of the final makefile (“final_makefile.yaml”) wa ...
Genotypic Detection of rpoB and katG Gene Mutations Associated
... Streptomycin (STR). Drug resistance and MDRTB strains of Mycobacterium tuberculosis have been recognized in many parts of the world (2,3,4), which hinders the success of TB control programs. MDR-TB is defined as the resistance to at least two main first line TB drugs: RIF and INH (1). Drug resistanc ...
... Streptomycin (STR). Drug resistance and MDRTB strains of Mycobacterium tuberculosis have been recognized in many parts of the world (2,3,4), which hinders the success of TB control programs. MDR-TB is defined as the resistance to at least two main first line TB drugs: RIF and INH (1). Drug resistanc ...
PDF - WashU Epigenome Browser
... Learn more about the supported numerical track formats bedGraph (http: //wiki.wubrowse.org/bedgraph) and bigWig (http://wiki.wubrowse.org/bigwig). ...
... Learn more about the supported numerical track formats bedGraph (http: //wiki.wubrowse.org/bedgraph) and bigWig (http://wiki.wubrowse.org/bigwig). ...
Rhom-2 Expression Does Not Always Correlate With
... ern blot was prepared using BamHI-digested DNA. Hybridization of the Southern blot with a TCR-p probez4is shown in Fig 1A. As controls, DNAs from the KB and HPB cell lines were included. A germline band of 23 kb and two smaller rearranged bands for the TCR-p gene were observed for the Kl3 and HPB ce ...
... ern blot was prepared using BamHI-digested DNA. Hybridization of the Southern blot with a TCR-p probez4is shown in Fig 1A. As controls, DNAs from the KB and HPB cell lines were included. A germline band of 23 kb and two smaller rearranged bands for the TCR-p gene were observed for the Kl3 and HPB ce ...
Alu Human Polymorphism
... inherited from each parent – Most occurred millions of years ago and are often on both pairs of chromosomes – There are Alu elements that have occurred since humans branched from other primates – This gives rise to dimorphic Alus from the last hundreds of thousands of years – Estimated to jump 1:200 ...
... inherited from each parent – Most occurred millions of years ago and are often on both pairs of chromosomes – There are Alu elements that have occurred since humans branched from other primates – This gives rise to dimorphic Alus from the last hundreds of thousands of years – Estimated to jump 1:200 ...
What Do You Mean, “Epigenetic”?
... a pronounced dichotomy within the field of epigenetics. Waddington’s epigenetics describes the interplay of genetic and cytoplasmic elements that produce emergent phenotypes (Van Speybroeck 2002; Jamniczky et al. 2010), and those in the biological sciences interested in gene-by-environment interactio ...
... a pronounced dichotomy within the field of epigenetics. Waddington’s epigenetics describes the interplay of genetic and cytoplasmic elements that produce emergent phenotypes (Van Speybroeck 2002; Jamniczky et al. 2010), and those in the biological sciences interested in gene-by-environment interactio ...
Impact of Tandem Repeats on the Scaling of Nucleotide Sequences
... ABSTRACT Techniques such as detrended fluctuation analysis (DFA) and its extensions have been widely used to determine the nature of scaling in nucleotide sequences. In this brief communication we show that tandem repeats which are ubiquitous in nucleotide sequences can prevent reliable estimation o ...
... ABSTRACT Techniques such as detrended fluctuation analysis (DFA) and its extensions have been widely used to determine the nature of scaling in nucleotide sequences. In this brief communication we show that tandem repeats which are ubiquitous in nucleotide sequences can prevent reliable estimation o ...
7. molecular genetics.
... Each time a somatic cell divides, two daughter cells are produced. Each of these cells receives an identical copy of the parent cell´s genetic information. ...
... Each time a somatic cell divides, two daughter cells are produced. Each of these cells receives an identical copy of the parent cell´s genetic information. ...
Yeast vectors for the integration/expression of any sequence at the
... sequences. This procedure (Langston and Symington, 2004; Hastings et al., 1993) generates the integration of the marker within the chromosomal target sequence but does not create sequence duplications, and thus increases the chromosomal stability of integrants. Yeast integrative vectors for general ...
... sequences. This procedure (Langston and Symington, 2004; Hastings et al., 1993) generates the integration of the marker within the chromosomal target sequence but does not create sequence duplications, and thus increases the chromosomal stability of integrants. Yeast integrative vectors for general ...
Risks from GMOs due to Horizontal Gene Transfer
... to all forms of gene transfer that do not involve parentto-offspring transfer (sexual or asexual). It can occur either naturally or by human intervention (e.g. gene technology, embryo rescue, in vitro fertilization, protoplast fusion, self-cloning). In some cases, HGT may be transient, and not perpet ...
... to all forms of gene transfer that do not involve parentto-offspring transfer (sexual or asexual). It can occur either naturally or by human intervention (e.g. gene technology, embryo rescue, in vitro fertilization, protoplast fusion, self-cloning). In some cases, HGT may be transient, and not perpet ...
Appendix 5 Laboratory Projects List
... A list of my lab’s projects is compiled and distributed once per semester and discussed at a “Research Updates” lab meeting (see Chapter 3). Prefixes like “*NIH*” identify funded projects and the project’s source of support. Initials in parentheses indicate who in the lab was working on the project. ...
... A list of my lab’s projects is compiled and distributed once per semester and discussed at a “Research Updates” lab meeting (see Chapter 3). Prefixes like “*NIH*” identify funded projects and the project’s source of support. Initials in parentheses indicate who in the lab was working on the project. ...
Télécharger - Options Méditerranéennes
... applied MAS, some technologies may not be appropriate for all applications (e.g. genome-wide genotyping chips would be an expensive way to follow a few key loci in a segregating population). In addition, new techniques such as TILLING (Target Induced Local Lesions In Genomes; Till et al., 2003) will ...
... applied MAS, some technologies may not be appropriate for all applications (e.g. genome-wide genotyping chips would be an expensive way to follow a few key loci in a segregating population). In addition, new techniques such as TILLING (Target Induced Local Lesions In Genomes; Till et al., 2003) will ...
Chapter 7: Genetics Lesson 4: Mutations
... Mistakes may occur during meiosis that result in nondisjunction. This is the failure of replicated chromosomes to separate during meiosis (the animation at the link below shows how this happens). Some of the resulting gametes will be missing a chromosome, while others will have an extra copy of the ...
... Mistakes may occur during meiosis that result in nondisjunction. This is the failure of replicated chromosomes to separate during meiosis (the animation at the link below shows how this happens). Some of the resulting gametes will be missing a chromosome, while others will have an extra copy of the ...
REPORT C-Terminal Deletions in the ALAS2 Gene Lead to Gain of
... shown) and ALAS2. Protoporphyrin accumulation segregated with an X chromosome haplotype defined by microsatellite markers around ALAS2 in three families (data not shown). Sequencing of genomic DNA identified two different deletions (c.1706–1709 delAGTG in six families; c.1699–1700 delAT in two familie ...
... shown) and ALAS2. Protoporphyrin accumulation segregated with an X chromosome haplotype defined by microsatellite markers around ALAS2 in three families (data not shown). Sequencing of genomic DNA identified two different deletions (c.1706–1709 delAGTG in six families; c.1699–1700 delAT in two familie ...
Chapter 7: Genetics Lesson 7.4: Mutations
... likely to result in a car that does not run well or perhaps does not run at all. By the same token, any random change in a gene’s DNA is likely to result in a protein that does not function normally or may not function at all. Such mutations are likely to be harmful. Harmful mutations may cause gene ...
... likely to result in a car that does not run well or perhaps does not run at all. By the same token, any random change in a gene’s DNA is likely to result in a protein that does not function normally or may not function at all. Such mutations are likely to be harmful. Harmful mutations may cause gene ...
Biology Lesson Plan - Penn Arts and Sciences
... The Human Genome Project, completed in 2003 funded by the U.S. Department of Energy and the National Institutes of Health, is a way for the science world to examine human DNA and identify the estimated 30,000 genes that are present. This research will be used for a variety of reasons that include: u ...
... The Human Genome Project, completed in 2003 funded by the U.S. Department of Energy and the National Institutes of Health, is a way for the science world to examine human DNA and identify the estimated 30,000 genes that are present. This research will be used for a variety of reasons that include: u ...
Database homology searching
... • Masking by hand: – If you know about the DNA binding domain already • Which is really common and will occupy the top 100 hits against any database ...
... • Masking by hand: – If you know about the DNA binding domain already • Which is really common and will occupy the top 100 hits against any database ...
Challenges of integrating conventional breeding and biotechnology
... ‘art’ because, essentially most of the selection for improved genetic type has been based on empirical selection of observed phenotypes and statistical analysis of observational plot data, rather than for the specific, desirable underlying genetic factors, the genes known to improve those traits. T ...
... ‘art’ because, essentially most of the selection for improved genetic type has been based on empirical selection of observed phenotypes and statistical analysis of observational plot data, rather than for the specific, desirable underlying genetic factors, the genes known to improve those traits. T ...
Recent progress on the Ada response for inducible repair of DNA
... levels of environmental alkylating agents, many bacteria mount an inducible response that enhances cellular resistance to these same agents. This adaptive response has been most extensively studied in E. coli, in which induced alkylation resistance results from increased expression of four genes, ad ...
... levels of environmental alkylating agents, many bacteria mount an inducible response that enhances cellular resistance to these same agents. This adaptive response has been most extensively studied in E. coli, in which induced alkylation resistance results from increased expression of four genes, ad ...
Hydrogen autotrophy of Nocardia opaca strains is
... method of Marmur (1961) these linear plasmids were not detectable; this may be due to their sensitivity to shearing forces. On conventional agarose gel electrophoresis the linear plasmids formed a broad band located slightly above the largest A HindIII fragment (Fig. 2). In lysates of N . opaca obta ...
... method of Marmur (1961) these linear plasmids were not detectable; this may be due to their sensitivity to shearing forces. On conventional agarose gel electrophoresis the linear plasmids formed a broad band located slightly above the largest A HindIII fragment (Fig. 2). In lysates of N . opaca obta ...
Cleavage of a model DNA replication fork by a Type I restriction
... replication fork crowding, as described below. Therefore, it is possible that some relationship exists between restriction cleavage and replication fork passage. Another relationship between Type I restriction and DNA replication is suggested by the restriction alleviation phenomena: the phenotypic ...
... replication fork crowding, as described below. Therefore, it is possible that some relationship exists between restriction cleavage and replication fork passage. Another relationship between Type I restriction and DNA replication is suggested by the restriction alleviation phenomena: the phenotypic ...