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A Sex Chromosome Rearrangement in a Human XX
A Sex Chromosome Rearrangement in a Human XX

... from the paternal X chromosome. However, one XX male inherited not only the entire pseudoautosomal region of the paternal Y but also a proximal portion of the pseudoautosomal region of the paternal X. In this XX male, the recombination point on the X is likely to lie within the pseudoautosomal regio ...
RECOMBINEERING: A POWERFUL NEW TOOL FOR MOUSE
RECOMBINEERING: A POWERFUL NEW TOOL FOR MOUSE

Highly precise and developmentally programmed genome
Highly precise and developmentally programmed genome

... lular eukaryotes, two kinds of nuclei coexist in the same cytoplasm [8]: the somatic macronucleus (MAC) is essential for gene expression but is destroyed at each sexual cycle, while the germline micronucleus (MIC) undergoes meiosis and transmits its genome to the zygotic nucleus. New MICs and MACs o ...
A novel environment-sensitive biodegradable polydisulfide with
A novel environment-sensitive biodegradable polydisulfide with

... plasmid DNA and siRNA. A novel polydisulfide with protonatable pendants was synthesized by the oxidative polymerization of a dithiol monomer, which was readily prepared by solid phase chemistry. The polydisulfide exhibited good buffering capacity and low cytotoxicity. It formed stable complexes with ...
E.Publication
E.Publication

... Maybe you say yes. However, the cost of this treatment for people who are albino may increase government’s expenditure on health for everyone. Would that change your answer? Think about the choices Fahim’s mother would have to make. If she loves Fahim the way he is, how does she explain a decision t ...
PDF
PDF

... component of epigenetics. It must be clarified that two types of epigenetic inheritance are usually referred to: (i) epigenetic marks, which can be inherited in the soma line as these marks are conserved during mitosis (Jablonka and Raz, 2009), and (ii) transgenerational epigenetic inheritance via th ...
General - Bioinformatics Research Group at SRI International
General - Bioinformatics Research Group at SRI International

... Click “Summary of Organisms”, then click organism name, then click “Pathway Evidence”, then click “Save Pathway Report” ...
1 Glossary 5` overhang- Restriction enzymes that cleave the DNA
1 Glossary 5` overhang- Restriction enzymes that cleave the DNA

... Circular permutation – The genomes of some bacteriophage always contain the same genes but they are not always present on the infecting phage in the same order. For example, one phage may have the order ABCDEFG, another may have CDEFGAB and another may have DEFGABC. These different phage genomes are ...
Bioremediation and Biomass Utilization
Bioremediation and Biomass Utilization

... transforming cellulosic biomass (plant fibers) into biofuels. Three areas where focused biological research can lead to much lower costs and increased productivity include developing crops dedicated to biofuel production (see step 1), engineering enzymes that deconstruct cellulosic biomass (see step ...
Microsoft Word Document
Microsoft Word Document

... Blue/white screen – The visual screen that is used to tell when a plasmid has a cloned insert. The blue color results from an -fragment of -galactosidase combining with an -fragment of -galactosidase to form an active -galactosidase molecule. When the -fragment is inactivated by a cloned inser ...
Bio 6 – DNA & Gene Expression Lab  Overview
Bio 6 – DNA & Gene Expression Lab Overview

... on to the next generation. In other words, you are about to learn what genetic information actually means, not just for human beings, but for all life on earth. Once it was known that genes are made of DNA which somehow codes for proteins, a number of scientists set out to uncover the underlying gen ...
BlastLecture8
BlastLecture8

... • Masking by hand: – If you know about the DNA binding domain already • Which is really common and will occupy the top 100 hits against any database ...
Site-Directed Mutagenesis Using Oligonucleotide
Site-Directed Mutagenesis Using Oligonucleotide

... Methods enabling mutational analysis of distinct chromosomal locations, like site-directed mutagenesis, insertion of foreign sequences or in-frame deletions, have become of fast growing interest since complete bacterial genome sequences became available. Various approaches have been described to mod ...
genstat - University of Illinois at Urbana
genstat - University of Illinois at Urbana

... A basic approach is to scan for ORFs whose length exceeds certain threshold – This is naïve because some genes (e.g. some neural and immune system genes) are relatively short ...
Molecular Genetics
Molecular Genetics

The Molecular Genetic Basis of Glanzmann`s
The Molecular Genetic Basis of Glanzmann`s

MOLLECULAR BIOLOGY COURSE
MOLLECULAR BIOLOGY COURSE

... functional molecules called proteins. (A few genes produce other molecules that help the cell assemble proteins.) The journey from gene to protein is complex and tightly controlled within each cell. It consists of two major steps: transcription and translation. Together, transcription and ...
Meiosis and Reproduction
Meiosis and Reproduction

... • DNA is “double helix”— two complementary strands wound in a spiral • Strands separate and DNA replicates by filling in other half of each separated strand • Famous Watson-Crick model (Nobel prize) ...
2007-10_GO-resources_jblake
2007-10_GO-resources_jblake

... The Gene Ontology is a controlled vocabulary of terms to describe gene product characteristics in the domains of localization and function. Databases using GO terms to annotate their genes and gene products can submit their annotations to the GO consortium where they are made freely available for ot ...
Plant Genome Resources at the National Center for Biotechnology
Plant Genome Resources at the National Center for Biotechnology

Pharmacogenomics Module Presentation
Pharmacogenomics Module Presentation

... nucleotides that encode for many genes. Gene RNA: A single-stranded copy of one gene. RNA Protein: Proteins are composed of amino acids. Amino acids are made from triplets of nucleotides called codons. ...
The Bacillus subtilis clpC operon encodes DNA
The Bacillus subtilis clpC operon encodes DNA

... T o examine the possibility that orf5 is indeed an sms homologue in B. subtilis, sensitivity to the alkylating agent MMS was tested using a paper disc method as described by Neuwald et al. (1990). Several independent experiments were performed by plating exponentially growing cultures of the BEKS (o ...
serious asthma should focus on the possible confounding role
serious asthma should focus on the possible confounding role

... others acquire asthma later in life. Still other patients develop the disease in early childhood and suffer from asthma their entire life. In addition, ethnicity, sex and age affect asthma susceptibility and severity [8, 9]. How can we explain these different patterns? A challenging hypothesis may b ...
Reverse Genetic Analysis of Terminal Ear
Reverse Genetic Analysis of Terminal Ear

... and nine in Arabidopsis thaliana. In this thesis, a programme of reverse genetic analysis has been designed to investigate if Arabidopsis genes most closely aligned in parsimony trees with TE1, TERMINAL EAR-LIKE 1 (TEL1), TERMINAL EAR-LIKE 2 (TEL2), perform the same function as TE1. The expression p ...
Your Genes, Your Choices
Your Genes, Your Choices

... is not albino. If it is, he and his wife could choose to have an abortion and try again. What do you think of this choice? Adoption is another choice Martin and his wife could make, instead of risking bearing children who are albino. With adoption, the children would not be their own, genetically. B ...
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Genome editing

Genome editing, or genome editing with engineered nucleases (GEEN) is a type of genetic engineering in which DNA is inserted, replaced, or removed from a genome using artificially engineered nucleases, or ""molecular scissors."" The nucleases create specific double-stranded break (DSBs) at desired locations in the genome, and harness the cell’s endogenous mechanisms to repair the induced break by natural processes of homologous recombination (HR) and nonhomologous end-joining (NHEJ). There are currently four families of engineered nucleases being used: Zinc finger nucleases (ZFNs), Transcription Activator-Like Effector Nucleases (TALENs), the CRISPR/Cas system, and engineered meganuclease re-engineered homing endonucleases.It is commonly practiced in genetic analysis that in order to understand the function of a gene or a protein function one interferes with it in a sequence-specific way and monitors its effects on the organism. However, in some organisms it is difficult or impossible to perform site-specific mutagenesis, and therefore more indirect methods have to be used, such as silencing the gene of interest by short RNA interference (siRNA) . Yet gene disruption by siRNA can be variable and incomplete. Genome editing with nucleases such as ZFN is different from siRNA in that the engineered nuclease is able to modify DNA-binding specificity and therefore can in principle cut any targeted position in the genome, and introduce modification of the endogenous sequences for genes that are impossible to specifically target by conventional RNAi. Furthermore, the specificity of ZFNs and TALENs are enhanced as two ZFNs are required in the recognition of their portion of the target and subsequently direct to the neighboring sequences.It was chosen by Nature Methods as the 2011 Method of the Year.
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