• Study Resource
  • Explore
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
Determination of the entire sequence of turtle CR1: the first open
Determination of the entire sequence of turtle CR1: the first open

... The putative zinc finger has cysteine residues with identical spacing and a similar amino acid composition to those found in the species-specific transcription initiation factors SLl and TIF-IB. The 5’ untranslated region (5’ UTR) of PsCRl contains a sequence similar to part of the human Ll promoter ...
ERF/AP2 Subfamily A3 and ER/AP2 Subfamily A6 Genes
ERF/AP2 Subfamily A3 and ER/AP2 Subfamily A6 Genes

... Enough plants were screened T-DNA was not inserted into the gene Fatality of knockout to seed development is inconclusive No further analysis can be done on these plants ...
Greedy Algorithms And Genome Rearrangements
Greedy Algorithms And Genome Rearrangements

... Turnip vs Cabbage: Almost Identical mtDNA gene sequences • In 1980s Jeffrey Palmer studied evolution of plants by comparing genomes of the cabbage and turnip • 99% similarity between genes • These surprisingly identical gene sequences differed in gene order • This study helped pave the way to analyz ...
Replication Protein A (RPA1a) Is Required for Meiotic and Somatic
Replication Protein A (RPA1a) Is Required for Meiotic and Somatic

... DNA double-strand break (DSB) repair is particularly important in maintaining the integrity of genome among individuals and shuffling genetic information among population, because DSBs are generated not only in meiotic cells but also from the action of certain endogenous or exogenous DNA-damaging ag ...
S4 Text.
S4 Text.

... Now that we have isolated good quality genomic DNA from our GAL4 enhancer trap lines, we can perform inverse PCR to identify the locations of the pGawB inserts. We will begin by digesting a portion of our genomic DNA with a restriction enzyme that cuts frequently, HpaII. Recombinant DNA technology w ...
Supplementary Notes S1 (doc 64K)
Supplementary Notes S1 (doc 64K)

... polymorphisms listed in the DGV (version- variation.hg18.v10.nov.2010) as described above. Primers were designed using Primer Express (Applied Biosystems) and purchased from Integrated DNA Technologies (www.idtdna.com) in lab ready format. The patient's DNA was diluted in PCR-grade water, and the qu ...
it is not in our genes
it is not in our genes

... its null hypothesis is an interesting issue. The main empirical evidence upon which the HGP hypothesis was based were familial studies of twins and to a lesser extent, adoptees. For example, twin studies find heritabilities of 50% or more for many major mental illnesses, like schizophrenia and bipol ...
Deletion of a Disease Resistance Nucleotide-Binding
Deletion of a Disease Resistance Nucleotide-Binding

... nucleotide-binding sites (NBS) and leucine-rich repeat regions (LRR). This class includes genes from dicotyledons as well as monocotyledons. Some members of this class contain an N-terminal coiled coil domain and others contain a toll-interleukin receptor-like domain (Dangl and Jones 2001; Mondragon ...
Ironing Out Jesse`s Problem
Ironing Out Jesse`s Problem

... Student correctly determines the answer and expresses it in a fraction, decimal, or percent. ...
statgen9
statgen9

... Different levels of expression of different tRNAs for a given amino acid lead to pressure on coding regions to “conform” to the preferred ...
Comparative Genomics of Microbes
Comparative Genomics of Microbes

... • Tandem repeats: regions of repeated DNA in immediate succession but with different copy number in different genomes. – A repeat can occur 2.5 times October 2K5 ...
Overview of milestones in genetics and genetic variation Author
Overview of milestones in genetics and genetic variation Author

... None of these or other theories however, could unravel the mystery of inheritance till 19th century. Geneticist believed that some kind of hereditary material exists in all living organisms and this material should fulfil three requirements:  Replication ability-It should be able to replicate so th ...
Tutorial - Maize Inflorescence
Tutorial - Maize Inflorescence

Full Text
Full Text

... refresh acquaintance with several common terms and basic genetic concepts (a brief glossary is provided in Table 1). Phenotype refers to the observable characteristics (or symptoms of illness) under consideration. Genotype refers to the set of genes an individual possesses that are relevant to the p ...
5. Harmful mutations
5. Harmful mutations

... In a cell, DNA replication begins at specific locations in the genome, called "origins". Unwinding of DNA at the origin, and synthesis of new strands, forms a replication fork. 5. DNA self-correction Imino-cytosine pairs (incorrectly, as far as the cell is concerned) with adenine. Nanoseconds later, ...
Restriction Enzyme digestion of DNA
Restriction Enzyme digestion of DNA

... • In addition to conformation affecting migration rate, laboratory production of plasmid DNA can be produce very large molecules that migrate very slowly. Two possible molecules that can be produced are dimers and concatemers. A dimer consists of two plasmids covalently linked in a series end to en ...
Cells: A Multiple Time Point Analysis Chronic Lymphocytic
Cells: A Multiple Time Point Analysis Chronic Lymphocytic

... To analyze the modalities of clonal expansion of chronic lymphocytic leukemia (CLL) cells, we sequenced at multiple time points the V(D)J genes expressed by CD51IgM1CLL B cells in three patients. All three V(D)J gene sequences were found to be point mutated. The mutation frequency in the Ig VH (3.96 ...
Document
Document

... from a single gene multiple transcripts) AS is tissue-specific (Graveley, 2001) AS is related to human diseases ...
Near Neutrality, Rate Heterogeneity, and Linkage Govern
Near Neutrality, Rate Heterogeneity, and Linkage Govern

... No other molecule features more prominently in vertebrate molecular systematics and evolution than mitochondrial DNA (mtDNA). From the restriction maps of the 1970s to the present-day currency of whole mitochondrial genome sequences, this genome has dominated the stage of the entire field of study n ...
Supplementary Information (doc 1117K)
Supplementary Information (doc 1117K)

... Kwok, C., Zeisig, B. B., Qiu, J., Dong, S., and So, C. W. (2009). Transforming activity of AML1-ETO is independent of CBFbeta and ETO interaction but requires formation of homooligomeric complexes. Proc Natl Acad Sci U S A 106, 2853-2858. Lukasik, S. M., Zhang, L., Corpora, T., Tomanicek, S., Li, Y ...
Conservation scores
Conservation scores

... • Original score is “rejected substitutions”: the number of substitutions expected under ‘neutrality’ minus the number of substitutions observed at each aligned position • New scores based on ML fit of substitution rate at base • Positive scores (fewer than expected) indicate that a site is ...
Enhanced Detection of Longer Insertions and Deletions in Clinical
Enhanced Detection of Longer Insertions and Deletions in Clinical

... of truncated proteins. However, accurate calling of indels larger than 20 base pairs (bp) using NGS based approaches for whole genome and whole exome analysis remains a challenge due to alignment errors, repeat sequences, incomplete reference genome, accuracy issues and unreliability of sequencing a ...
Transcriptional Deregulation of Mutated BCL6 Alleles by Loss of
Transcriptional Deregulation of Mutated BCL6 Alleles by Loss of

... The BCL6 proto-oncogene encodes a POZ/zinc finger transcriptional repressor expressed in germinal center (GC) B cells and required for GC formation. In ~35% of diffuse large B cell lymphomas (DLBCL) and 5–14% of follicular lymphomas (FL), the BCL6 locus is altered by chromosomal translocations that ...
The DNA sequence of the fragment Hind.30, 378 bases lcng, fran
The DNA sequence of the fragment Hind.30, 378 bases lcng, fran

... ill vitro transcriptianal terminator sites and a sequence of 171 bases which probably codes for the N terminus of the T7 RNA polymerase. The sequence also codes for the RNase III cleavage site before gene 1. This overlaps with the transcriptianal terminators. The RNA transcript of the sequence about ...
Target selected insertional mutagenesis on chromosome IV of
Target selected insertional mutagenesis on chromosome IV of

... Jones et al., 1994; Okuley et al., 1994; AzpirozLeehan and Feldmann, 1997). Insertional mutagenesis can also be used to identify insertions in specific target genes with known sequence but unknown function. This reverse genetics procedure termed target selected insertional mutagenesis uses T-DNA or ...
< 1 ... 79 80 81 82 83 84 85 86 87 ... 445 >

Genome editing

Genome editing, or genome editing with engineered nucleases (GEEN) is a type of genetic engineering in which DNA is inserted, replaced, or removed from a genome using artificially engineered nucleases, or ""molecular scissors."" The nucleases create specific double-stranded break (DSBs) at desired locations in the genome, and harness the cell’s endogenous mechanisms to repair the induced break by natural processes of homologous recombination (HR) and nonhomologous end-joining (NHEJ). There are currently four families of engineered nucleases being used: Zinc finger nucleases (ZFNs), Transcription Activator-Like Effector Nucleases (TALENs), the CRISPR/Cas system, and engineered meganuclease re-engineered homing endonucleases.It is commonly practiced in genetic analysis that in order to understand the function of a gene or a protein function one interferes with it in a sequence-specific way and monitors its effects on the organism. However, in some organisms it is difficult or impossible to perform site-specific mutagenesis, and therefore more indirect methods have to be used, such as silencing the gene of interest by short RNA interference (siRNA) . Yet gene disruption by siRNA can be variable and incomplete. Genome editing with nucleases such as ZFN is different from siRNA in that the engineered nuclease is able to modify DNA-binding specificity and therefore can in principle cut any targeted position in the genome, and introduce modification of the endogenous sequences for genes that are impossible to specifically target by conventional RNAi. Furthermore, the specificity of ZFNs and TALENs are enhanced as two ZFNs are required in the recognition of their portion of the target and subsequently direct to the neighboring sequences.It was chosen by Nature Methods as the 2011 Method of the Year.
  • studyres.com © 2025
  • DMCA
  • Privacy
  • Terms
  • Report