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UNIFR Rusconi 2002
UNIFR Rusconi 2002

... micro-array-based resequencing (size independent time) single nucleotide polymorphism detection techniques ultra-microscope-based sequencing (SNP) direct polymerase output sequencing ...
Genomic instability — an evolving hallmark of cancer
Genomic instability — an evolving hallmark of cancer

Nomenclature I
Nomenclature I

... families should be used where possible. A stem (or root) symbol as a basis for a symbol series allows easy identification of other family members in both database searches and the literature. Gene family members should be designated by Arabic numerals placed immediately after the gene stem symbol, w ...
Extraction of correlated gene clusters from multiple genomic data by
Extraction of correlated gene clusters from multiple genomic data by

... extract sets of genes which share similarities with respect to multiple biological attributes. The originality of our approach is the extension of the concept of correlation for non-vectorial data, which is made possible by the use of generalized kernel canonical correlation analysis (KCCA), and the ...
Chapter 13 Unintended Horizontal Transfer of Recombinant DNA
Chapter 13 Unintended Horizontal Transfer of Recombinant DNA

... organism involved (e.g. sexual reproduction/inheritance by descent). This process is called vertical gene transfer and an example is pollen flow between the same or related plant species.1 Thus, vertical gene transfer is the normal mode in which DNA is shared among individuals and passed on to the f ...
Exercise 11 - Understanding the Output for a blastn Search
Exercise 11 - Understanding the Output for a blastn Search

... When we design a BLAST search, there are three basic decisions we must make: the BLAST program we wish to use, the query sequence we want to annotate, and the database we want to search. In addition, there are several optional parameters (such as the ‘expect’ threshold and other scoring parameters) ...
PPT - Blumberg Lab
PPT - Blumberg Lab

... Genome wide analysis of gene function • Loss-of-function analysis is the most powerful way to identify gene function – Direct link between genotype and phenotype – Forward vs reverse genetics • Forward genetics-> random mutagenesis followed by phenotypic analysis – Identity of gene involved not kno ...
Evolutionary dynamics of full genome content in Escherichia coli
Evolutionary dynamics of full genome content in Escherichia coli

... sequences are often acquired from organisms of very ...
Modulation of base excision repair of 8
Modulation of base excision repair of 8

... Construction of plasmid containing clustered 8-oxoG Covalently closed circular plasmid DNA containing a Cp[8-oxoG]/Cp[8oxoG] bistranded clustered lesion was constructed by sequential exchange of native fragments of each of the DNA strands for matching synthetic oligonucleotides, each containing sing ...
Word - The Open University
Word - The Open University

... There is no point making haemoglobin in skin cells, or in producing in blood cells the protein that CF patients need in the lining of their lungs. And ideally it would have to go on working, perhaps in the cells’ descendants, otherwise repeat treatments would be needed. The idea of, in effect, treat ...
Lecture25
Lecture25

... The next step: bring in the sequence • Genome-wide “location data” or “binding data” combined with sequence data • For each TF, collect all sequences bound by it – These are promoter length sequences, not exact binding sites ...
Sequence analysis of three mitochondrial DNA molecules reveals
Sequence analysis of three mitochondrial DNA molecules reveals

... genes are transcribed together as one unit (1,41). This organisation is also seen in other yeasts, like C.glabrata, where co-transcription also occurs (42), and in Saccharomyces exiguus (43) and Kluyveromyces lactis (44). In S.castellii the same tRNA genes are separated by 627 bp of which at least 5 ...
AthaMap web tools for database-assisted identification of
AthaMap web tools for database-assisted identification of

CHARACTERlZATION OF THE ~ 0 CHONDRIA . L DNA MOLECULE
CHARACTERlZATION OF THE ~ 0 CHONDRIA . L DNA MOLECULE

Apolipoprotein A5, a Newly Identified Gene That Affects Plasma
Apolipoprotein A5, a Newly Identified Gene That Affects Plasma

... apolipoproteins. To define the true in vivo function of APOA5, 2 different engineered mouse lines were generated.8 First, a human 26-kb XhoI restriction fragment was isolated that was predicted to contain only APOA5 and its flanking sequence. This genomic piece of DNA was subsequently used to genera ...
Rate Asymmetry After Genome Duplication Causes Substantial
Rate Asymmetry After Genome Duplication Causes Substantial

... and A2 are two subsets of the A-type trees of Langkjaer et al. (2003), the distinction being that in A1 trees the S. kluyveri sequence clusters with the slower evolving of the two S. cerevisiae ohnologs, whereas in A2 trees the S. kluyveri gene clusters with the faster S. cerevisiae ohnolog. As befo ...
Apolipoprotein A5, a Newly Identified Gene That Affects Plasma
Apolipoprotein A5, a Newly Identified Gene That Affects Plasma

... apolipoproteins. To define the true in vivo function of APOA5, 2 different engineered mouse lines were generated.8 First, a human 26-kb XhoI restriction fragment was isolated that was predicted to contain only APOA5 and its flanking sequence. This genomic piece of DNA was subsequently used to genera ...
No Slide Title
No Slide Title

... Libraries a collection of clones representing the entire complement of sequences of interest 1) entire genome for genomic libraries 2) all mRNA for cDNA ...
Nucleotide Sequence Preservation of Human
Nucleotide Sequence Preservation of Human

... sequence comparisons of the D-loop region of unrelated normal humans (5, 21). Three additional between-individual differences outside the Dloop region of mtDNA were identified in this study. Substitution of cytidine for thymidine at L-strand positions 9698 and 9725 was found in all clones containing ...
Bioinformatics Course Notes (Ming Li)
Bioinformatics Course Notes (Ming Li)

... transcription ...
Chapter 3. The Beginnings of Genomic Biology
Chapter 3. The Beginnings of Genomic Biology

... formulation of a structure for DNA did not involve biologists. Rather Irwin Chargaff, an analytical, organic chemist, and physicists, Rosalind Franklin and Maurice Wilkins made the laboratory observations that led to the solution of the structure of DNA. Chargaff determined that there were 4 differe ...
Synthesizing double haploid hexaploid wheat populations based on
Synthesizing double haploid hexaploid wheat populations based on

... in genetic recombination with the genomes of interest and will be homogenous in all the DH derivatives of a particular cross. For example, if we are interested in analyzing QTLs in the A and B genomes of T. turgidum, we can use tetraploid wheat to make the first hybrids and Ae. tauschii as the alien ...
- GenoSensor Corporation
- GenoSensor Corporation

... are what characterize the differences between alleles. There are 3 known snips in the gene TAS2R38. The most common one, located at the 785 nucleotide position of the DNA template strand, is associated with a loss of function in the protein product. This particular snip is a transition mutation from ...
ISEcp1 pneumoniae  Research Article
ISEcp1 pneumoniae Research Article

... (CTX-M-1, -3, -10, -11, -12, -15, -22, -23, -27, -28, -29, -30, -32, -33, 34, -36, -37 and -42). The second and third higher genes found prevalent in this study was TEM type β-lactamases with 24.8% isolates positive in PCR and SHV type β-lactamases with 7.8 % positive. A recent study from India on m ...
12864_2008_1659_MOESM3_ESM
12864_2008_1659_MOESM3_ESM

... (each encoding 15 aa) (Figure S3). The protein repeat domain aligns to the titin (TTN) protein (e-value 3x10-23), featuring many repeats and contains a tandem Ig cluster (Radke et al. 2007). The C-terminus encoding part of TXNDC2 does not align to TTN, and thus, the novel gene is composed of two fus ...
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Genome editing

Genome editing, or genome editing with engineered nucleases (GEEN) is a type of genetic engineering in which DNA is inserted, replaced, or removed from a genome using artificially engineered nucleases, or ""molecular scissors."" The nucleases create specific double-stranded break (DSBs) at desired locations in the genome, and harness the cell’s endogenous mechanisms to repair the induced break by natural processes of homologous recombination (HR) and nonhomologous end-joining (NHEJ). There are currently four families of engineered nucleases being used: Zinc finger nucleases (ZFNs), Transcription Activator-Like Effector Nucleases (TALENs), the CRISPR/Cas system, and engineered meganuclease re-engineered homing endonucleases.It is commonly practiced in genetic analysis that in order to understand the function of a gene or a protein function one interferes with it in a sequence-specific way and monitors its effects on the organism. However, in some organisms it is difficult or impossible to perform site-specific mutagenesis, and therefore more indirect methods have to be used, such as silencing the gene of interest by short RNA interference (siRNA) . Yet gene disruption by siRNA can be variable and incomplete. Genome editing with nucleases such as ZFN is different from siRNA in that the engineered nuclease is able to modify DNA-binding specificity and therefore can in principle cut any targeted position in the genome, and introduce modification of the endogenous sequences for genes that are impossible to specifically target by conventional RNAi. Furthermore, the specificity of ZFNs and TALENs are enhanced as two ZFNs are required in the recognition of their portion of the target and subsequently direct to the neighboring sequences.It was chosen by Nature Methods as the 2011 Method of the Year.
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